Name: Cohnella saccharovorans CJ22
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141089

Type strain:

Species: Cohnella saccharovorans

Strain Designation: CJ22

Culture col. no.: KACC 17501, JCM 19227

Strain history: C.-J. Cha CJ22.

NCBI tax ID(s): 1255256 (species)

Section

Cohnella saccharovorans CJ22 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from soil of a ginseng field located in Anseong, Korea.

colony-forming
Gram-positive
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Cohnella*
	Species	**Cohnella saccharovorans**
	Full Scientific Name (PNU)	Cohnella saccharovorans Choi et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43994]	Gram stain	positive
[Ref.: #43994]	Cell length	2 µm
[Ref.: #43994]	Cell width	0.5 µm
[Ref.: #43994]	Cell shape	rod-shaped
[Ref.: #43994]	Motility	yes
[Ref.: #43994]	Flagellum arrangement	peritrichous

[Ref.: #43994]	Colony color	creamy white
[Ref.: #43994]	Colony shape	circular
[Ref.: #43994]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43994]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43994]

Culture medium growth

		Temperature
[Ref.: #43994]	growth	20-37 °C
[Ref.: #43994]	optimum	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #43994]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43994]			optimum	7
[Ref.: #43994]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43994]

Oxygen tolerance

aerobe

[Ref.: #43994]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43994]		NaCl		growth	0-1	%(w/v)
[Ref.: #43994]		NaCl		optimum	0	%(w/v)

[Ref.: #43994]	Observation	quinone MK-7
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43994]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43994]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #43994]	adipate ChEBI	-	builds acid from
[Ref.: #43994]	amygdalin ChEBI	+	builds acid from
[Ref.: #43994]	arabinose ChEBI	-	builds acid from
[Ref.: #43994]	arbutin ChEBI	+	builds acid from
[Ref.: #43994]	arginine ChEBI	-	builds acid from
[Ref.: #43994]	casein	-	hydrolysis
[Ref.: #43994]	cellobiose ChEBI	+	builds acid from
[Ref.: #43994]	D-arabinose ChEBI	+	builds acid from
[Ref.: #43994]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43994]	D-fructose ChEBI	+	builds acid from
[Ref.: #43994]	D-fucose ChEBI	-	builds acid from
[Ref.: #43994]	D-galactose ChEBI	+	builds acid from
[Ref.: #43994]	D-glucose ChEBI	+	builds acid from
[Ref.: #43994]	D-lyxose ChEBI	+	builds acid from
[Ref.: #43994]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43994]	D-mannose ChEBI	+	builds acid from
[Ref.: #43994]	D-ribose ChEBI	+	builds acid from
[Ref.: #43994]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43994]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43994]	D-xylose ChEBI	+	builds acid from
[Ref.: #43994]	decanoate ChEBI	-	builds acid from
[Ref.: #43994]	dna ChEBI	-	hydrolysis
[Ref.: #43994]	erythritol ChEBI	-	builds acid from
[Ref.: #43994]	esculin ChEBI	+	hydrolysis
[Ref.: #43994]	galactitol ChEBI	+	builds acid from
[Ref.: #43994]	gelatin ChEBI	-	hydrolysis
[Ref.: #43994]	gentiobiose ChEBI	+	builds acid from
[Ref.: #43994]	gluconate ChEBI	-	builds acid from
[Ref.: #43994]	glucose ChEBI	-	builds acid from
[Ref.: #43994]	glycerol 3-phosphate ChEBI	-	builds acid from
[Ref.: #43994]	glycogen ChEBI	+	builds acid from
[Ref.: #43994]	inulin ChEBI	+	builds acid from
[Ref.: #43994]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43994]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43994]	L-fucose ChEBI	+	builds acid from
[Ref.: #43994]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #43994]	L-sorbose ChEBI	-	builds acid from
[Ref.: #43994]	L-xylose ChEBI	-	builds acid from
[Ref.: #43994]	lactose ChEBI	+	builds acid from
[Ref.: #43994]	maleic acid ChEBI	-	builds acid from
[Ref.: #43994]	maltose ChEBI	-	builds acid from
[Ref.: #43994]	maltose ChEBI	+	builds acid from
[Ref.: #43994]	mannitol ChEBI	-	builds acid from
[Ref.: #43994]	mannose ChEBI	-	builds acid from
[Ref.: #43994]	melezitose ChEBI	+	builds acid from
[Ref.: #43994]	melibiose ChEBI	+	builds acid from
[Ref.: #43994]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #43994]	methyl alpha-D-mannoside ChEBI	+	builds acid from
[Ref.: #43994]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from
[Ref.: #43994]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43994]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #43994]	nitrate ChEBI	-	reduction
[Ref.: #43994]	phenylacetate ChEBI	-	builds acid from
[Ref.: #43994]	raffinose ChEBI	+	builds acid from
[Ref.: #43994]	ribitol ChEBI	-	builds acid from
[Ref.: #43994]	salicin ChEBI	+	builds acid from
[Ref.: #43994]	starch ChEBI	+	hydrolysis
[Ref.: #43994]	sucrose ChEBI	+	builds acid from
[Ref.: #43994]	trehalose ChEBI	+	builds acid from
[Ref.: #43994]	turanose ChEBI	+	builds acid from
[Ref.: #43994]	urea ChEBI	+	hydrolysis
[Ref.: #43994]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #43994]		indole ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #43994]	2-nitrophenyl beta-D-galactopyranoside	+	3.2.1.52
[Ref.: #43994]	acid phosphatase	-	3.1.3.2
[Ref.: #43994]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43994]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43994]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43994]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43994]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43994]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43994]	beta-galactosidase	+	3.2.1.23
[Ref.: #43994]	beta-glucosidase	+	3.2.1.21
[Ref.: #43994]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43994]	catalase	+	1.11.1.6
[Ref.: #43994]	cystine arylamidase	-	3.4.11.3
[Ref.: #43994]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43994]	esterase (C 4)	+
[Ref.: #43994]	esterase Lipase (C 8)	+
[Ref.: #43994]	leucine arylamidase	+	3.4.11.1
[Ref.: #43994]	lipase (C 14)	-
[Ref.: #43994]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43994]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #43994]	trypsin	-	3.4.21.4
[Ref.: #43994]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43994]

Fatty acid	Percentage
C15:0 anteiso	42
C16:0 iso	30.2
C16:0	11.2
C17:0 anteiso	4.8
C14:0 iso	4.5
C14:0	2.3
C15:0 iso	2.3

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43994]	Sample type/isolated from	soil of a ginseng field located in Anseong, Korea
[Ref.: #43994]	Geographic location (country and/or sea, region)	Anseong
[Ref.: #43994]	Country	Republic of Korea
[Ref.: #43994]	Country ISO 3 Code	KOR
[Ref.: #43994]	Continent	Asia
[Ref.: #43994]	Enrichment culture	R2A agar
[Ref.: #43994]	Enrichment culture duration	3 days
[Ref.: #43994]	Enrichment culture temperature	30 ̊C

[Ref.: #67770]	Sample type/isolated from	Soil of a ginseng field located in Anseong
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43994]	16S rRNA gene sequence	JQ806733		GenBank

[Ref.: #67770]

GC-content

63.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43994]

Culture collection no.

KACC 17501, JCM 19227

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cohnella saccharovorans sp. nov., isolated from ginseng soil.	Choi JH, Seok JH, Jang HJ, Cha JH, Cha CJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000933	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43994

Jung-Hye Choi, Ji-Hye Seok, Ho-Jin Jang, Ju-Hee Cha, Chang-Jun Cha: Cohnella saccharovorans sp. nov., isolated from ginseng soil. IJSEM 66: 1887 - 1893 2016 ( DOI 10.1099/ijsem.0.000933 , PubMed 26813106 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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