Name: Shewanella hanedai 281
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14060

Type strain:

Species: Shewanella hanedai

Strain Designation: 281

Culture col. no.: DSM 6066, ATCC 33224, CCUG 16097, JCM 20706, ACAM 540, CIP 103207, IAM 12641, LMG 19004, LMG 2868, NBRC 102223, NCIMB 2157

Strain history: CIP <- 1988, NCMB <- ATCC <- P. Baumann: strain 281, Alteromonas hanedai <- K.H. Nealson <- J. Baross

NCBI tax ID(s): 25 (species)

SI-ID 12891

LMG 2868, ATCC 33224, LMG 19004, CECT 5017, CIP 103207, CCUG 16097, NCMB 2157, NCIMB 2157, CECT 5194, KCTC 2681, IAM 12641, NBRC 102223, JCM 20706, DSM 6066

Other strains from Shewanella hanedai

S. hanedai CCUG 16098, ATCC 33255
S. hanedai CCUG 16099
S. hanedai CCUG 16100
S. hanedai CCUG 16101
S. hanedai CCUG 16102, ATCC 35256

Section

Shewanella hanedai 281 is an obligate aerobe, psychrophilic, motile bacterium that was isolated from arctic sediment.

motile
obligate aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Alteromonadales*
	Family	*Shewanellaceae*
	Genus	*Shewanella*
	Species	**Shewanella hanedai**
	Full Scientific Name (LPSN)	Shewanella hanedai (Jensen et al. 1981) MacDonell and Colwell 1986

Synonym

Alteromonas hanedai

Information on morphological and physiological properties Morphology

[Ref.: #119833]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.984

[Ref.: #119833]	Cell shape	rod-shaped

[Ref.: #119833]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %98.945

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2427]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #2427]

Culture medium growth

[Ref.: #2427]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2427]

Culture medium composition

expand / minimize

[Ref.: #41339]

Culture medium

Marine agar (MA)

[Ref.: #41339]

Culture medium growth

[Ref.: #41339]

Culture medium composition

expand / minimize

[Ref.: #119833]

Culture medium

CIP Medium 13

[Ref.: #119833]

Culture medium growth

[Ref.: #119833]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2427]		growth	14 °C
[Ref.: #41339]		growth	22 °C
[Ref.: #46442]		growth	15 °C
[Ref.: #67770]		growth	15 °C
[Ref.: #119833]		growth	5-22 °C
[Ref.: #119833]	no	growth	25 °C
[Ref.: #119833]	no	growth	30 °C
[Ref.: #119833]	no	growth	37 °C
[Ref.: #119833]	no	growth	41 °C

[Ref.: #2427]	Temperature range	psychrophilic
[Ref.: #41339]	Temperature range	psychrophilic
[Ref.: #46442]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	psychrophilic
[Ref.: #119833]	Temperature range	psychrophilic
[Ref.: #119833]	Temperature range	mesophilic
[Ref.: #119833]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #119833]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.961

	Salt	Tested relation		Salt conc.
[Ref.: #119833]	NaCl		growth	2-4	%
[Ref.: #119833]	NaCl	no	growth	0	%
[Ref.: #119833]	NaCl	no	growth	6	%
[Ref.: #119833]	NaCl	no	growth	8	%
[Ref.: #119833]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119833]	citrate ChEBI	-	carbon source
[Ref.: #119833]	esculin ChEBI	-	hydrolysis
[Ref.: #119833]	nitrate ChEBI	+	reduction
[Ref.: #119833]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #119833]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119833]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119833]	amylase	-
[Ref.: #119833]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119833]	caseinase	+	3.4.21.50
[Ref.: #119833]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119833]	gelatinase	+
[Ref.: #119833]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119833]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119833]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119833]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119833]	oxidase	+
[Ref.: #119833]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119833]	tryptophan deaminase	-
[Ref.: #119833]	tween esterase	+
[Ref.: #119833]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #46442]

Fatty acid	Percentage	ECL
C16:1 ω7c	15.4	15.819
C16:0	13.8	16
C13:0 iso	12.4	12.612
C14:0	11.7	14
Unidentified	8.9
C12:0	7.5	12
C15:0	7.1	15
C13:0 ISO 3OH	6.2	14.11
C15:0 ISO	4.6	14.621
C12:0 3OH	3.8	13.455
C13:0	2.7	13
C17:1 ω8c	1.3	16.792
C15:1 ω8c	1.1	14.792
C13:0 ISO 2OH	1	13.814
C16:1 ω9c	1	15.774
C13:0 3OH/C15:1 i I/H	0.8	14.469
C17:0	0.6	17

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #119833]	360	not determinedn.d.	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119833]	7430	-	+	-	+	-	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2427]	Sample type/isolated from	arctic sediment

[Ref.: #46442]	Sample type/isolated from	Arctic sediment

[Ref.: #67770]	Sample type/isolated from	Arctic sediment

[Ref.: #119833]	Sample type/isolated from	Environment, Arctic sediment
[Ref.: #119833]	Geographic location (country and/or sea, region)	Arctic ocean
[Ref.: #119833]	Country	Arctic
[Ref.: #119833]	Continent	Arctic
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Sediment
#Condition	#Psychrophilic (<10°C)	-
#Climate	#Cold	#Polar

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X82132 (>99% sequence identity) for Shewanella from Microbeatlas

Aquatic Samples	10
Soil Samples
Animal Samples	25
Plant Samples
Total Samples	35

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2427]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119833]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2427]	S.hanedai 16S rRNA gene (CIP 103207T)	X82132	1442	ENA	25 tax ID
[Ref.: #20218]	Shewanella hanedai strain ATCC 33224 16S ribosomal RNA gene, partial sequence	DQ011269	1312	ENA	25 tax ID	*
[Ref.: #20218]	Shewanella hanedai ATCC 33224 16S ribosomal RNA gene, partial sequence	U91590	1351	ENA	25 tax ID	*
[Ref.: #20218]	Shewanella hanedai gene for 16S rRNA, partial sequence, strain: NBRC 102223	AB681737	1472	ENA	25 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Shewanella hanedai JCM 20706	GCA_007197645	contig	GenBank	25 tax ID
[Ref.: #66792]	Shewanella hanedai JCM 20706	GCA_014647575	scaffold	GenBank	25 tax ID	*
[Ref.: #66792]	Shewanella hanedai strain JCM 20706	25.17	wgs	PATRIC	25 tax ID	*
[Ref.: #66792]	Shewanella hanedai strain JCM 20706	25.8	wgs	PATRIC	25 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Shewanella hanedai strain JCM 20706 PATRIC: 25.8
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Shewanella hanedai JCM 20706 NCBI: GCA_014647575.1
[Ref.: #69480]	flagellated	yes	85.763	no
[Ref.: #69480]	gram-positive	no	98.621	no
[Ref.: #69480]	anaerobic	no	98.183	no
[Ref.: #69480]	aerobic	yes	56.79	no
[Ref.: #69480]	halophile	yes	51.587	no
[Ref.: #69480]	spore-forming	no	96.719	no
[Ref.: #69480]	glucose-util	yes	92.032	no
[Ref.: #69480]	thermophile	no	99.796	yes
[Ref.: #69480]	motile	yes	93.21	no
[Ref.: #69480]	glucose-ferment	no	53.27	no

Availability in culture collections External links

[Ref.: #2427]

Culture collection no.

DSM 6066, ATCC 33224, CCUG 16097, JCM 20706, ACAM 540, CIP 103207, IAM 12641, LMG 19004, LMG 2868, NBRC 102223, NCIMB 2157

[Ref.: #83229]

SI-ID 12891

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis.	Vogel BF, Jorgensen K, Christensen H, Olsen JE, Gram L	Appl Environ Microbiol	10.1128/aem.63.6.2189-2199.1997	1997	*

References

#2427

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6066

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41339 ; Curators of the CIP;
#46442 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 16097

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83229

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12891.1 )

#119833 Collection of Institut Pasteur ; Curators of the CIP; CIP 103207
* These data were automatically processed and therefore are not curated

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Shewanella hanedai strain JCM 20706

Shewanella hanedai JCM 20706

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Change proposal