Shewanella hanedai (DSM 6066, ATCC 33224, CCUG 16097)

Name: Shewanella hanedai (DSM 6066, ATCC 33224, CCUG 16097)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14060

Type strain

Strain Designation 281

Culture col. no. DSM 6066 ATCC 33224 CCUG 16097 281 NCIMB 2157 7 more

NCBI tax ID(s) 25

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- ATCC <- P. Baumann, 281 (Alteromonas hanedai) <- K.H. Nealson <- J. Baross JCM <-- IAM 12641 <-- ATCC 33224 <-- P. Baumann 281 <-- K. H. Nealson <-- J. Baross. CIP <- 1988, NCMB <- ATCC <- P. Baumann: strain 281, Alteromonas hanedai <- K.H. Nealson <- J. Baross

Links

Shewanella hanedai 281 is an obligate aerobe, Gram-negative, motile bacterium that was isolated from arctic sediment.

Gram-negative motile rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 12891

LMG 2868,ATCC 33224,LMG 19004,CECT 5017,CIP 103207,CCUG 16097,NCMB 2157,NCIMB 2157,CECT 5194,KCTC 2681,IAM 12641,NBRC 102223,JCM 20706,DSM 6066

@ref 20215

Last LPSN update 2026-05-29 08:28:11

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Alteromonadales

Family Shewanellaceae

Genus Shewanella

Species Shewanella hanedai

Full scientific name Shewanella hanedai (Jensen et al. 1981) MacDonell and Colwell 1986

Synonyms (1)

Alteromonas hanedai

BacDive ID	Other strains from **Shewanella hanedai** (5)	Type strain
143007	S. hanedai CCUG 16098, ATCC 33255
143008	S. hanedai CCUG 16099
143009	S. hanedai CCUG 16100
143010	S. hanedai CCUG 16101
143011	S. hanedai CCUG 16102, ATCC 35256

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
125438			90.317
125439	negative		97.753
125438	negative		99.5
119833	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2427	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
41339	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
119833	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
2427	positive	growth	14
41339	positive	growth	22
46442	positive	growth	15
67770	positive	growth	15
119833	positive	growth	5-22
119833	negative	growth	25
119833	negative	growth	30
119833	negative	growth	37
119833	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
119833	obligate aerobe
125439	obligate aerobe	92.167

Spore formation

@ref	Spore formation	Confidence
125438		90.049
125439		99.915

Halophily

@ref	Salt	Growth	Tested relation	Concentration
119833	NaCl	positive	growth	2-4 %
119833	NaCl		growth	0 %
119833	NaCl		growth	6 %
119833	NaCl		growth	8 %
119833	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
119833	16947 ChEBI	citrate	-	carbon source
119833	4853 ChEBI	esculin	-	hydrolysis
119833	17632 ChEBI	nitrate	+	reduction
119833	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119833	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119833	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119833	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
119833	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
119833	caseinase	+	3.4.21.50
119833	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
119833	gelatinase	+
119833	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
119833	lipase	+
68382	lipase (C 14)	-		from API zym
119833	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119833	ornithine decarboxylase	-	4.1.1.17
119833	oxidase	+
119833	protease	+
68382	trypsin	-	3.4.21.4	from API zym
119833	tryptophan deaminase	-
119833	tween esterase	+
119833	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

46442

Fatty acid	Percentage	ECL
C16:1 ω7c	15.4	15.819
C16:0	13.8	16
C13:0 iso	12.4	12.612
C14:0	11.7	14
Unidentified	8.9
C12:0	7.5	12
C15:0	7.1	15
C13:0 ISO 3OH	6.2	14.11
C15:0 ISO	4.6	14.621
C12:0 3OH	3.8	13.455
C13:0	2.7	13
C17:1 ω8c	1.3	16.792
C15:1 ω8c	1.1	14.792
C13:0 ISO 2OH	1	13.814
C16:1 ω9c	1	15.774
C13:0 3OH/C15:1 i I/H	0.8	14.469
C17:0	0.6	17

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119833	-	+	-	+	-	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119833	not determinedn.d.	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Sediment
#Condition	#Psychrophilic (<10°C)	-
#Climate	#Cold	#Polar

Isolation

@ref	Sample type	Geographic location	Country	Continent
2427	arctic sediment
46442	Arctic sediment
67770	Arctic sediment
119833	Environment, Arctic sediment	Arctic ocean	Arctic	Arctic

Taxonmaps

69479

Global distribution of 16S sequence X82132 (>99% sequence identity) for Shewanella from Microbeatlas

Aquatic Samples	10
Soil Samples	0
Animal Samples	25
Plant Samples	0
Total Samples	35

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2427	1	Risk group (German classification) According to TRBA 466
119833	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM719764v1 assembly for Shewanella hanedai JCM 20706	contig	GCA_007197645	25.8	2996183579	25	61.46
66792	ASM1464757v1 assembly for Shewanella hanedai JCM 20706	scaffold	GCA_014647575	25.17	8003806220	25	41.44

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Shewanella hanedai strain ATCC 33224 16S ribosomal RNA gene, partial sequence	DQ011269	1312	25
20218	Shewanella hanedai ATCC 33224 16S ribosomal RNA gene, partial sequence	U91590	1351	25
20218	Shewanella hanedai gene for 16S rRNA, partial sequence, strain: NBRC 102223	AB681737	1472	25
2427	S.hanedai 16S rRNA gene (CIP 103207T)	X82132	1442	25

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM719764v1 assembly for Shewanella hanedai JCM 20706
NCBI: GCA_007197645

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	92.17	no
125439	gram_stain	BacteriaNetⓘ	negative	97.75	no
125439	motility	BacteriaNetⓘ	yes	71.29	no
125439	spore_formation	BacteriaNetⓘ	no	99.92	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Shewanella hanedai JCM 20706
NCBI: GCA_014647575.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.50	no
125438	anaerobic	anaerobicⓘ	no	92.49	no
125438	aerobic	aerobicⓘ	no	51.55	no
125438	spore-forming	spore-formingⓘ	no	90.05	no
125438	thermophilic	thermophileⓘ	no	98.00	yes
125438	flagellated	motile2+ⓘ	yes	90.32	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Nucleotide sequences of 5S ribosomal RNAs from three marine eubacteria: Shewanella hanedai, Alteromonas colwelliana and Vibrio mediterranei.	Ortiz-Conde BA, Muir DG, Pillidge CJ, Gobius KS, Anikis MS, Powell DM, Hori H, Colwell RR.	Nucleic Acids Res	10.1093/nar/17.12.4881	1989
Phylogeny	Isolation and identification of Photobacterium phosphoreum from an unexpected niche: migrating salmon.	Budsberg KJ, Wimpee CF, Braddock JF.	Appl Environ Microbiol	10.1128/aem.69.11.6938-6942.2003	2003
Enzymology	Design and application of rRNA-targeted oligonucleotide probes for the dissimilatory iron- and manganese-reducing bacterium Shewanella putrefaciens.	DiChristina TJ, DeLong EF.	Appl Environ Microbiol	10.1128/aem.59.12.4152-4160.1993	1993
Metabolism	Shewanella spp. genomic evolution for a cold marine lifestyle and in-situ explosive biodegradation.	Zhao JS, Deng Y, Manno D, Hawari J.	PLoS One	10.1371/journal.pone.0009109	2010
	Evolutionary relationships of cultivated psychrophilic and barophilic deep-sea bacteria.	Delong EF, Franks DG, Yayanos AA.	Appl Environ Microbiol	10.1128/aem.63.5.2105-2108.1997	1997
	Compilation of 5S rRNA and 5S rRNA gene sequences.	Specht T, Wolters J, Erdmann VA.	Nucleic Acids Res	10.1093/nar/18.suppl.2215	1990
Phylogeny	Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis.	Vogel BF, Jorgensen K, Christensen H, Olsen JE, Gram L	Appl Environ Microbiol	10.1128/aem.63.6.2189-2199.1997	1997

References

#2427	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6066
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#41339	; Curators of the CIP;
#46442	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 16097
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119833	Collection of Institut Pasteur ; Curators of the CIP; CIP 103207
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Shewanella hanedai (DSM 6066, ATCC 33224, CCUG 16097)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_007197645

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media