Name: Flavobacterium olei R-10-9
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140564

Type strain:

Species: Flavobacterium olei

Strain Designation: R-10-9

Culture col. no.: JCM 31673, KACC 18997, KEMB 9005-447

Strain history: J. Kim; Kyonggi Univ., South Korea; R-10-9.

NCBI tax ID(s): 1886782 (species)

Section

Flavobacterium olei R-10-9 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from oil-contaminated soil from Ulaanbaatar, Mongolia.

colony-forming
Gram-negative
rod-shaped
aerobe
psychrophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Flavobacterium*
	Species	**Flavobacterium olei**
	Full Scientific Name (LPSN)	Flavobacterium olei Chaudhary and Kim 2017

Information on morphological and physiological properties Morphology

[Ref.: #43293]	Gram stain	negative

[Ref.: #43293]	Cell length	1.1-1.4 µm

[Ref.: #43293]	Cell width	0.3-0.5 µm

[Ref.: #43293]	Cell shape	rod-shaped

[Ref.: #43293]	Motility	no

[Ref.: #43293]	Colony size	1.0-1.5 mm
[Ref.: #43293]	Colony color	translucent yellow
[Ref.: #43293]	Colony shape	circular
[Ref.: #43293]	Incubation period	5 days
[Ref.: #43293]	Cultivation medium used	R2A Agar (MB Cell)

[Ref.: #43293]	Pigment production	yes
[Ref.: #43293]	Pigment name	Flexirubin-Type

Information on culture and growth conditions Culture and growth conditions

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Culture medium

R2A Agar (MB Cell)

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Culture medium growth

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Culture medium

R2A (MB Cell)

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Culture medium growth

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Culture medium

tryptone-soya agar (Oxoid)

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Culture medium growth

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Culture medium

Nutrient agar (Oxoid)

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

veal infusion agar (Difco)

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Culture medium growth

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Culture medium

MacConkey agar (Oxoid)

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Culture medium growth

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Culture medium

marine agar 2216 (Difco)

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Culture medium growth

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Culture medium

brain-heart infusion agar

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #43293]	no	growth	(-2)-(-1) °C
[Ref.: #43293]	no	growth	33-40 °C
[Ref.: #43293]		optimum	10-25 °C
[Ref.: #43293]		growth	0-32 °C
[Ref.: #67770]		growth	10 °C

[Ref.: #43293]	Temperature range	psychrophilic
[Ref.: #67770]	Temperature range	psychrophilic

	Kind of pH		pH
[Ref.: #43293]		optimum	7-8
[Ref.: #43293]		growth	5-9.5
[Ref.: #43293]	no	growth	4-4.5
[Ref.: #43293]	no	growth	10-12

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43293]		NaCl		growth	0-1	%
[Ref.: #43293]		NaCl		maximum	0	%

[Ref.: #43293]	Observation	production of brown pigments in tyrosine agar
[Ref.: #67770]	Observation	quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43293]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #43293]	3-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #43293]	4-hydroxybutyrate ChEBI	-	assimilation
[Ref.: #43293]	4-nitrophenyl beta-D-galactopyranoside ChEBI	+	hydrolysis
[Ref.: #43293]	adipate ChEBI	-	assimilation
[Ref.: #43293]	carboxymethylcellulose ChEBI	+	hydrolysis
[Ref.: #43293]	casein	+	hydrolysis
[Ref.: #43293]	crab shell chitin	-	hydrolysis
[Ref.: #43293]	D-mannitol ChEBI	-	assimilation
[Ref.: #43293]	D-mannose ChEBI	+	assimilation
[Ref.: #43293]	D-ribose ChEBI	-	assimilation
[Ref.: #43293]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43293]	decanoate ChEBI	-	assimilation
[Ref.: #43293]	dna ChEBI	-	hydrolysis
[Ref.: #43293]	esculin ChEBI	+	hydrolysis
[Ref.: #43293]	esculin ferric citrate	+	hydrolysis
[Ref.: #43293]	gelatin ChEBI	-	fermentation
[Ref.: #43293]	gelatin ChEBI	-	hydrolysis
[Ref.: #43293]	glucose ChEBI	+	assimilation
[Ref.: #43293]	glucose ChEBI	-	fermentation
[Ref.: #43293]	glycogen ChEBI	-	assimilation
[Ref.: #43293]	itaconate ChEBI	-	assimilation
[Ref.: #43293]	L-alanine ChEBI	-	assimilation
[Ref.: #43293]	L-arabinose ChEBI	+	assimilation
[Ref.: #43293]	L-arginine ChEBI	-	fermentation
[Ref.: #43293]	L-fucose ChEBI	-	assimilation
[Ref.: #43293]	L-histidine ChEBI	-	assimilation
[Ref.: #43293]	L-proline ChEBI	-	assimilation
[Ref.: #43293]	L-rhamnose ChEBI	+	assimilation
[Ref.: #43293]	L-serine ChEBI	-	assimilation
[Ref.: #43293]	lactate ChEBI	-	assimilation
[Ref.: #43293]	malate ChEBI	-	assimilation
[Ref.: #43293]	maltose ChEBI	+	assimilation
[Ref.: #43293]	melibiose ChEBI	-	assimilation
[Ref.: #43293]	myo-inositol ChEBI	-	assimilation
[Ref.: #43293]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43293]	nitrate ChEBI	-	reduction
[Ref.: #43293]	phenylacetate ChEBI	-	assimilation
[Ref.: #43293]	potassium 2-dehydro-D-gluconate	-	assimilation
[Ref.: #43293]	potassium 5-dehydro-D-gluconate	+	assimilation
[Ref.: #43293]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43293]	propionate ChEBI	-	assimilation
[Ref.: #43293]	salicin ChEBI	-	assimilation
[Ref.: #43293]	sodium acetate ChEBI	-	assimilation
[Ref.: #43293]	sodium citrate ChEBI	-	assimilation
[Ref.: #43293]	sodium malonate ChEBI	-	assimilation
[Ref.: #43293]	starch ChEBI	-	hydrolysis
[Ref.: #43293]	suberic acid ChEBI	-	assimilation
[Ref.: #43293]	sucrose ChEBI	+	assimilation
[Ref.: #43293]	tween 40 ChEBI	+	hydrolysis
[Ref.: #43293]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43293]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43293]	urea ChEBI	-	fermentation
[Ref.: #43293]	valerate ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43293]	ampicillin ChEBI	yes	30	µg (disc)
[Ref.: #43293]	chloramphenicol ChEBI	yes	100	µg (disc)
[Ref.: #43293]	cycloheximide ChEBI				yes	30	µg (disc)
[Ref.: #43293]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43293]	kanamycin ChEBI				yes	30	µg (disc)
[Ref.: #43293]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #43293]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #43293]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43293]	penicillin ChEBI	yes	10	µg (disc)
[Ref.: #43293]	rifampicin ChEBI				yes	10	µg (disc)
[Ref.: #43293]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #43293]	sulfamethoxazole ChEBI				yes	30	µg (disc)
[Ref.: #43293]	tetracycline ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Group ID of combined antibiotics	Metabolite	Antibiotic resistance	Concentration
[Ref.: #43293]		26	sulfamethoxazole ChEBI	yes	30	µg (disc)
[Ref.: #43293]		26	trimethoprim ChEBI	yes	30	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #43293]		acetoin ChEBI	no
[Ref.: #43293]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #43293]		acetoin ChEBI	-
[Ref.: #43293]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #43293]	acid phosphatase	+	3.1.3.2
[Ref.: #43293]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43293]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43293]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43293]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43293]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43293]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43293]	beta-galactosidase	+	3.2.1.23
[Ref.: #43293]	beta-glucosidase	+	3.2.1.21
[Ref.: #43293]	beta-glucuronidase	+/-	3.2.1.31
[Ref.: #43293]	catalase	+	1.11.1.6
[Ref.: #43293]	cystine arylamidase	-	3.4.11.3
[Ref.: #43293]	esterase (C 4)	+
[Ref.: #43293]	esterase Lipase (C 8)	+
[Ref.: #43293]	leucine arylamidase	-	3.4.11.1
[Ref.: #43293]	lipase (C 14)	-
[Ref.: #43293]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43293]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43293]	trypsin	+/-	3.4.21.4
[Ref.: #43293]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43293]	Sample type/isolated from	oil-contaminated soil from Ulaanbaatar, Mongolia
[Ref.: #43293]	Geographic location (country and/or sea, region)	Mongolia
[Ref.: #43293]	Country	Mongolia
[Ref.: #43293]	Country ISO 3 Code	MNG
[Ref.: #43293]	Continent	Asia
[Ref.: #43293]	Enrichment culture	R2A-Agar (MB Cell)

[Ref.: #67770]	Sample type/isolated from	Oil-contaminated soil from Ulaanbaatar
[Ref.: #67770]	Country	Mongolia
[Ref.: #67770]	Country ISO 3 Code	MNG
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43293]	Flavobacterium sp. R-10-9 16S ribosomal RNA gene, partial sequence	KX672808	1445	GenBank	1886782 tax ID

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GC-content

35.9 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

JCM 31673, KACC 18997, KEMB 9005-447

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flavobacterium olei sp. nov., a novel psychrotolerant bacterium isolated from oil-contaminated soil.	Chaudhary DK, Kim J	Int J Syst Evol Microbiol	10.1099/ijsem.0.001925	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43293

Dhiraj Kumar Chaudhary and Jaisoo Kim: Flavobacterium olei sp. nov., a novel psychrotolerant bacterium isolated from oil-contaminated soil. IJSEM 67: 2211 - 2218 2017 ( DOI 10.1099/ijsem.0.001925 , PubMed 28699576 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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