Strain identifier

BacDive ID: 140564

Type strain: Yes

Species: Flavobacterium olei

Strain Designation: R-10-9

Strain history: J. Kim; Kyonggi Univ., South Korea; R-10-9.

NCBI tax ID(s): 1886782 (species)

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General

@ref: 43293

BacDive-ID: 140564

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped, colony-forming

description: Flavobacterium olei R-10-9 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from oil-contaminated soil from Ulaanbaatar, Mongolia.

NCBI tax id

  • NCBI tax id: 1886782
  • Matching level: species

strain history

  • @ref: 67770
  • history: J. Kim; Kyonggi Univ., South Korea; R-10-9.

doi: 10.13145/bacdive140564.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium olei
  • full scientific name: Flavobacterium olei Chaudhary and Kim 2017

@ref: 43293

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium olei

strain designation: R-10-9

type strain: yes

Morphology

cell morphology

  • @ref: 43293
  • gram stain: negative
  • cell length: 1.1-1.4 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43293
  • colony size: 1.0-1.5 mm
  • colony color: translucent yellow
  • colony shape: circular
  • incubation period: 5 days
  • medium used: R2A Agar (MB Cell)

pigmentation

  • @ref: 43293
  • production: yes
  • name: Flexirubin-Type

Culture and growth conditions

culture medium

@refnamegrowth
43293R2A Agar (MB Cell)yes
43293R2A (MB Cell)yes
43293tryptone-soya agar (Oxoid)yes
43293Nutrient agar (Oxoid)yes
43293LB (Luria-Bertani) MEDIUMyes
43293veal infusion agar (Difco)yes
43293MacConkey agar (Oxoid)no
43293marine agar 2216 (Difco)no
43293brain-heart infusion agarno

culture temp

@refgrowthtypetemperaturerange
43293nogrowth(-2)-(-1)psychrophilic
43293nogrowth33-40
43293positiveoptimum10-25
43293positivegrowth0-32
67770positivegrowth10psychrophilic

culture pH

@refabilitytypepHPH range
43293positivegrowth5-9.5alkaliphile
43293positiveoptimum7-8
43293nogrowth4-4.5
43293nogrowth10-12

Physiology and metabolism

oxygen tolerance

  • @ref: 43293
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43293
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43293NaClpositivegrowth0-1 %
43293NaClpositivemaximum0 %

observation

@refobservation
43293production of brown pigments in tyrosine agar
67770quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4329318186tyrosine-hydrolysis
43293esculin ferric citrate+hydrolysis
432933557154-nitrophenyl beta-D-galactopyranoside+hydrolysis
4329317632nitrate-reduction
4329316467L-arginine-fermentation
4329316199urea-fermentation
432935291gelatin-fermentation
4329317234glucose+assimilation
4329316024D-mannose+assimilation
4329317234glucose-fermentation
43293casein+hydrolysis
4329353423tween 40+hydrolysis
4329353426tween 80+hydrolysis
4329385146carboxymethylcellulose+hydrolysis
432934853esculin+hydrolysis
432935291gelatin-hydrolysis
4329328017starch-hydrolysis
4329316991dna-hydrolysis
43293crab shell chitin-hydrolysis
4329332032potassium gluconate-assimilation
4329317128adipate-assimilation
4329325115malate-assimilation
4329318401phenylacetate-assimilation
4329362345L-rhamnose+assimilation
43293506227N-acetylglucosamine+assimilation
4329317992sucrose+assimilation
4329317306maltose+assimilation
43293potassium 5-dehydro-D-gluconate+assimilation
4329330849L-arabinose+assimilation
4329316988D-ribose-assimilation
4329317268myo-inositol-assimilation
4329317240itaconate-assimilation
432939300suberic acid-assimilation
4329362983sodium malonate-assimilation
4329332954sodium acetate-assimilation
4329324996lactate-assimilation
4329316977L-alanine-assimilation
4329328087glycogen-assimilation
43293161933-hydroxybenzoate-assimilation
4329317115L-serine-assimilation
4329316899D-mannitol-assimilation
4329317814salicin-assimilation
4329328053melibiose-assimilation
4329318287L-fucose-assimilation
4329317924D-sorbitol-assimilation
4329317272propionate-assimilation
4329327689decanoate-assimilation
4329331011valerate-assimilation
4329353258sodium citrate-assimilation
4329315971L-histidine-assimilation
43293potassium 2-dehydro-D-gluconate-assimilation
43293370543-hydroxybutyrate-assimilation
43293167244-hydroxybutyrate-assimilation
4329317203L-proline-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.group ID
4329328971ampicillinyesyes30 µg (disc)
4329317698chloramphenicolyesyes100 µg (disc)
4329327641cycloheximideyesyes30 µg (disc)
4329317833gentamicinyesyes10 µg (disc)
432936104kanamycinyesyes30 µg (disc)
43293100147nalidixic acidyesyes30 µg (disc)
432937507neomycinyesyes30 µg (disc)
4329328368novobiocinyesyes30 µg (disc)
4329317334penicillinyesyes10 µg (disc)
4329328077rifampicinyesyes10 µg (disc)
4329317076streptomycinyesyes10 µg (disc)
432939332sulfamethoxazoleyesyes30 µg (disc)
4329327902tetracyclineyesyes30 µg (disc)
4329345924trimethoprimyesyes30 µg (disc)26
432939332sulfamethoxazoleyesyes30 µg (disc)26

metabolite production

@refChebi-IDmetaboliteproduction
4329335581indoleno
4329315688acetoinno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
4329317234glucose-
4329315688acetoin-

enzymes

@refvalueactivityec
43293catalase+1.11.1.6
43293alkaline phosphatase+3.1.3.1
43293esterase (C 4)+
43293esterase Lipase (C 8)+
43293acid phosphatase+3.1.3.2
43293naphthol-AS-BI-phosphohydrolase+
43293alpha-galactosidase+3.2.1.22
43293beta-galactosidase+3.2.1.23
43293alpha-glucosidase+3.2.1.20
43293beta-glucosidase+3.2.1.21
43293N-acetyl-beta-glucosaminidase+3.2.1.52
43293trypsin+/-3.4.21.4
43293beta-glucuronidase+/-3.2.1.31
43293lipase (C 14)-
43293leucine arylamidase-3.4.11.1
43293valine arylamidase-
43293cystine arylamidase-3.4.11.3
43293alpha-chymotrypsin-3.4.21.1
43293alpha-mannosidase-3.2.1.24
43293alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment culture
43293oil-contaminated soil from Ulaanbaatar, MongoliaMongoliaMongoliaMNGAsiaR2A-Agar (MB Cell)
67770Oil-contaminated soil from UlaanbaatarMongoliaMNGAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination#Oil (Fuel)
#Environmental#Terrestrial#Soil

Sequence information

16S sequences

  • @ref: 43293
  • description: Flavobacterium sp. R-10-9 16S ribosomal RNA gene, partial sequence
  • accession: KX672808
  • length: 1445
  • database: nuccore
  • NCBI tax ID: 1886782

GC content

  • @ref: 43293
  • GC-content: 35.9
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43293

culture collection no.: JCM 31673, KACC 18997, KEMB 9005-447

literature

  • topic: Phylogeny
  • Pubmed-ID: 28699576
  • title: Flavobacterium olei sp. nov., a novel psychrotolerant bacterium isolated from oil-contaminated soil.
  • authors: Chaudhary DK, Kim J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001925
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Mongolia, Nucleic Acid Hybridization, *Petroleum Pollution, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43293Dhiraj Kumar Chaudhary and Jaisoo KimFlavobacterium olei sp. nov., a novel psychrotolerant bacterium isolated from oil-contaminated soil10.1099/ijsem.0.001925IJSEM 67: 2211-2218 201728699576
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/