Name: Olleya algicola 3Alg 18
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140563

Type strain:

Species: Olleya algicola

Strain Designation: 3Alg 18

Culture col. no.: KCTC 22024, KMM 6133, DSM 109192

Strain history: <- OI Nedashkovskaya, Pacific Institute of Bioorganic Chemistry, Russia

NCBI tax ID(s): 2020714 (species)

SI-ID 405429

DSM 109192

Section

Olleya algicola 3Alg 18 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from isolated from the green alga Ulva fenestrata collected from Troitsa Bay, Gulf of Peter the Great, Sea of Japan.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Olleya*
	Species	**Olleya algicola**
	Full Scientific Name (LPSN)	Olleya algicola Nedashkovskaya et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43302]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #43302]	Cell length	1.0-2.7 µm

[Ref.: #43302]	Cell width	0.2-0.5 µm

[Ref.: #43302]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43302]	Motility	yes
[Ref.: #67771]	Motility	yes

[Ref.: #43302]	Flagellum arrangement	gliding
[Ref.: #67771]	Flagellum arrangement	gliding

[Ref.: #43302]	Colony size	2-3 mm
[Ref.: #43302]	Colony color	yellow
[Ref.: #43302]	Colony shape	circular
[Ref.: #43302]	Incubation period	2 days
[Ref.: #43302]	Cultivation medium used	MA (Difco)

[Ref.: #43302]	Pigment production	no
[Ref.: #43302]	Pigment name	Flexirubin-Type

Multimedia content

[Ref.: #64840]

Caption:

Iridescence (glitter-like) of DSM 109192 on medium 514f at 28 °C.

[Ref.: #64840]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43302]

Culture medium

marine agar (Difco)

[Ref.: #43302]

Culture medium growth

[Ref.: #64840]

Culture medium

MARINE BROTH (ROTH CP73) (DSMZ Medium 514f)

[Ref.: #64840]

Culture medium growth

[Ref.: #64840]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64840]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43302]	optimum	25-28 °C
[Ref.: #43302]	growth	4-37 °C
[Ref.: #64840]	growth	28 °C
[Ref.: #67771]	growth	25-28 °C

[Ref.: #43302]	Temperature range	mesophilic
[Ref.: #64840]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43302]			optimum	7.5
[Ref.: #43302]			growth	5.5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43302]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43302]		NaCl		growth	0.5-5	%(w/v)
[Ref.: #43302]		NaCl		optimum	3-4	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43302]	adipate ChEBI	+	assimilation
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43302]	agar ChEBI	-	hydrolysis
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #43302]	casein	+	hydrolysis
[Ref.: #43302]	cellobiose ChEBI	-	builds acid from
[Ref.: #43302]	cephalexin ChEBI	+
[Ref.: #43302]	chitin ChEBI	-	hydrolysis
[Ref.: #43302]	citrate ChEBI	-	builds acid from
[Ref.: #43302]	D-fructose ChEBI	-	builds acid from
[Ref.: #43302]	D-galactose ChEBI	-	builds acid from
[Ref.: #43302]	D-glucose ChEBI	+	builds acid from
[Ref.: #43302]	D-glucose ChEBI	+	assimilation
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #43302]	D-mannitol ChEBI	+	assimilation
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #43302]	D-mannose ChEBI	+	builds acid from
[Ref.: #43302]	D-mannose ChEBI	+	assimilation
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43302]	D-xylose ChEBI	-	builds acid from
[Ref.: #43302]	D-xylose ChEBI	+	assimilation
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43302]	dna ChEBI	-	hydrolysis
[Ref.: #43302]	esculin ChEBI	+	hydrolysis
[Ref.: #43302]	esculin ChEBI	+	assimilation
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #43302]	gelatin ChEBI	+	hydrolysis
[Ref.: #68369]	gelatin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #43302]	gluconate ChEBI	+	assimilation
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43302]	glycerol ChEBI	-	builds acid from
[Ref.: #43302]	glycogen ChEBI	+	assimilation
[Ref.: #43302]	hydrogen sulfide ChEBI	-
[Ref.: #43302]	L-arabinose ChEBI	-	builds acid from
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #43302]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43302]	lactose ChEBI	-	builds acid from
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #43302]	maltose ChEBI	+	builds acid from
[Ref.: #43302]	maltose ChEBI	+	assimilation
[Ref.: #43302]	maltose	+	assimilation
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #43302]	mannitol ChEBI	-	builds acid from
[Ref.: #43302]	melibiose ChEBI	-	builds acid from
[Ref.: #43302]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43302]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #43302]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #43302]	raffinose ChEBI	-	builds acid from
[Ref.: #43302]	ribose ChEBI	-	builds acid from
[Ref.: #43302]	sodium citrate ChEBI	+	assimilation
[Ref.: #43302]	sorbitol ChEBI	-	builds acid from
[Ref.: #43302]	starch ChEBI	+	hydrolysis
[Ref.: #43302]	sucrose ChEBI	-	builds acid from
[Ref.: #43302]	trehalose ChEBI	-	builds acid from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #43302]	tween 20 ChEBI	+	hydrolysis
[Ref.: #43302]	tween 40 ChEBI	+	hydrolysis
[Ref.: #43302]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43302]	urea ChEBI	-	hydrolysis
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43302]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #43302]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #43302]	cefazolin ChEBI	yes	30	µg (disc)
[Ref.: #43302]	cephalexin ChEBI	yes	30	µg (disc)
[Ref.: #43302]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43302]	doxycycline ChEBI	yes	10	µg (disc)
[Ref.: #43302]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #43302]	gentamicin ChEBI	no			no	10	µg (disc)
[Ref.: #43302]	kanamycin ChEBI				no	30	µg (disc)
[Ref.: #43302]	lincomycin ChEBI	yes	15	µg (disc)
[Ref.: #43302]	nalidixic acid ChEBI				yes	30	µg (disc)
[Ref.: #43302]	neomycin ChEBI				yes	30	µg (disc)
[Ref.: #43302]	ofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #43302]	oleandomycin ChEBI	yes	15	µg (disc)
[Ref.: #43302]	oxacillin ChEBI				yes	10	µg (disc)
[Ref.: #43302]	penicillin g ChEBI	yes	10	Unit
[Ref.: #43302]	polymyxin b ChEBI				yes	300	Unit
[Ref.: #43302]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #43302]	streptomycin ChEBI				yes	30	µg (disc)
[Ref.: #43302]	tetracycline ChEBI	yes	5	µg (disc)
[Ref.: #43302]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43302]	acetoin ChEBI	yes
[Ref.: #43302]	hydrogen sulfide ChEBI	no
[Ref.: #43302]	indole ChEBI	no
[Ref.: #68369]	indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #43302]		acetoin ChEBI	+
[Ref.: #68369]		indole ChEBI		-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #43302]	acid phosphatase	+	3.1.3.2
[Ref.: #43302]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43302]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43302]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43302]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43302]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43302]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #43302]	beta-glucosidase	+	3.2.1.21
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #43302]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43302]	cystine arylamidase	+
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #43302]	esterase (C 4)	-	3.1.1.1
[Ref.: #43302]	esterase Lipase (C 8)	+	3.1.1.3
[Ref.: #68369]	gelatinase	+		* from API 20NE
[Ref.: #43302]	leucine arylamidase	+
[Ref.: #43302]	lipase (C 14)	-	3.1.1.3
[Ref.: #43302]	lysine decarboxylase	-	4.1.1.18
[Ref.: #43302]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43302]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43302]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #43302]	trypsin	+	3.4.21.4
[Ref.: #43302]	tryptophan deaminase	-	4.1.99.1
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #43302]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43302]

Fatty acid	Percentage
C15:0 iso	17.2
C15:1 iso G	16.6
C16:0 iso 2OH	14.6
C15:0 iso 3OH	14.6
C17:0 iso 3OH	12.9
iso-15:0 2-OH / C16:1 ω7c	11.6
C16:0 iso 3OH	6.3
C15:0 anteiso	5.5
C15:0	5.2
C15:1 ω6c	3
C15:1 anteiso	1.4
C16:1 iso	1.1

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #64840]	24806	-	-	-	-	-	+	+	-	+	-	+	-	-	+	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43302]	Sample type/isolated from	isolated from the green alga Ulva fenestrata collected from Troitsa Bay, Gulf of Peter the Great, Sea of Japan
[Ref.: #43302]	Geographic location (country and/or sea, region)	Gulf of Peter the Great
[Ref.: #43302]	Country	Russia
[Ref.: #43302]	Country ISO 3 Code	RUS
[Ref.: #43302]	Continent	Asia
[Ref.: #43302]	Geographic location	42.0000°/131.0000°
[Ref.: #43302]	Enrichment culture	Marine broth (Difco)
[Ref.: #43302]	Enrichment culture duration	7 days
[Ref.: #43302]	Enrichment culture temperature	28 ̊C

[Ref.: #64840]	Sample type/isolated from	green alga Ulva fenestrata<\i>
[Ref.: #64840]	Geographic location (country and/or sea, region)	East Sea, Gulf of Peter the Great, Troitsa Bay (42° 37' 21'' N, 131° 08' 06'' E)
[Ref.: #64840]	Country	Russia
[Ref.: #64840]	Country ISO 3 Code	RUS
[Ref.: #64840]	Continent	Europe

[Ref.: #67771]	Sample type/isolated from	From green alga `Ulva fenestrata`
[Ref.: #67771]	Geographic location (country and/or sea, region)	Troitsa Bay, Gulf of Peter the Great, East Sea(Sea of Japa), Pacific Ocean
[Ref.: #67771]	Country	Russia
[Ref.: #67771]	Country ISO 3 Code	RUS
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Algae	#Green algae

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY341922 (>99% sequence identity) for Olleya from Microbeatlas

Aquatic Samples	889
Soil Samples	25
Animal Samples	57
Plant Samples	5
Total Samples	976

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64840]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64840]	Olleya algicola strain 3Alg 18 16S ribosomal RNA gene, partial sequence	KY341922	1470	ENA	2020714 tax ID

[Ref.: #67771]

GC-content

34.6 mol%

Availability in culture collections External links

[Ref.: #64840]

Culture collection no.

KCTC 22024, KMM 6133, DSM 109192

[Ref.: #96741]

SI-ID 405429

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Olleya algicola sp. nov., a marine bacterium isolated from the green alga Ulva fenestrata.	Nedashkovskaya OI, Kim SG, Zhukova NV, Mikhailov VV	Int J Syst Evol Microbiol	10.1099/ijsem.0.001926	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43302

Olga I. Nedashkovskaya, Song-Gun Kim,Natalia V. Zhukova and Valery V. Mikhailov: Olleya algicola sp. nov., a marine bacterium isolated from the green alga Ulva fenestrata. IJSEM 67: 2205 - 2210 2017 ( DOI 10.1099/ijsem.0.001926 , PubMed 28699861 )

#64840

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 109192

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96741

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405429.1 )

* These data were automatically processed and therefore are not curated

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