Bombella apis (JCM 31623, KCTC 52452, MRM 1)

Name: Bombella apis (JCM 31623, KCTC 52452, MRM 1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140538

Type strain

Culture col. no. JCM 31623 KCTC 52452 MRM 1

NCBI tax ID(s) 1785988

Special Collections JCM KCTC Literature strains

History J.-W. Bae; Dept. of Biol., Kyung Hee Univ., South Korea; MRM1. <- Jin-Woo Bae, Kyung Hee Univ.

Links

Bombella apis JCM 31623 is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from midgut of the honey bee in South Korea.

Gram-negative rod-shaped colony-forming obligate aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 272759

LMG 30666,KCTC 52452,JCM 31623

@ref 20215

Last LPSN update 2026-02-09 11:33:39

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodospirillales

Family Acetobacteraceae

Genus Bombella

Species Bombella apis

Full scientific name Bombella apis Yun et al. 2017

Synonyms (1)

"Parasaccharibacter apium"

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43324	negative	1.0-2.5 µm	0.5-0.6 µm	rod-shaped

Colony morphology

@ref	Colony color	Colony shape	Medium used
43324	creamy beige	circular	MRS agar

Culture and growth conditions

Culture medium

@ref	Name	Growth
43324	MRS agar
43324	Mannitol agar
43324	0,35% acetic acid agar
43324	LB (Luria-Bertani) MEDIUM
43324	Tryptic soy agar

Culture temp

@ref	Growth	Type	Temperature (°C)
43324	positive	optimum	30-37
67770	positive	growth	30
67771	positive	growth	30-37

Culture pH

@ref	Ability	Type	PH	PH range
43324	positive	optimum	7-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
43324	obligate aerobe
67771	aerobe

Spore formation

43324

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43324	NaCl	positive	growth	0 %(w/v)

Observation

@ref	Observation
43324	growth in the presence of 30% (w/v) glucose
43324	ubiquinone Q-10
67770	quinones: Q-10

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43324	58143 ChEBI	5-dehydro-D-gluconate	+	builds acid from
43324	30089 ChEBI	acetate	+/-	oxidation
43324	17925 ChEBI	alpha-D-glucose	+	carbon source
43324	17925 ChEBI	alpha-D-glucose	+	energy source
43324	28938 ChEBI	ammonium	-	growth
43324		ammonium	-	nitrogen source
43324	17108 ChEBI	D-arabinose	-	builds acid from
43324	15824 ChEBI	D-fructose	+	builds acid from
43324	15824 ChEBI	D-fructose	+	carbon source
43324	15824 ChEBI	D-fructose	+	energy source
43324	28847 ChEBI	D-fucose	+	builds acid from
43324	12936 ChEBI	D-galactose	+	builds acid from
43324	17634 ChEBI	D-glucose	+	builds acid from
43324	16899 ChEBI	D-mannitol	-	builds acid from
43324	16024 ChEBI	D-mannose	+	builds acid from
43324	17924 ChEBI	D-sorbitol	-	builds acid from
43324	65327 ChEBI	D-xylose	+	builds acid from
43324	16236 ChEBI	ethanol	-	oxidation
43324	16236 ChEBI	ethanol	-	builds acid from
43324	28066 ChEBI	gentiobiose	+	builds acid from
43324	17754 ChEBI	glycerol	+	builds acid from
43324	30849 ChEBI	L-arabinose	+	builds acid from
43324	17203 ChEBI	L-proline	+	carbon source
43324	17203 ChEBI	L-proline	+	energy source
43324	24996 ChEBI	lactate	-	oxidation
43324	28053 ChEBI	melibiose	+	builds acid from
43324	17790 ChEBI	methanol	-	carbon source
43324	16634 ChEBI	raffinose	-	builds acid from
43324	17992 ChEBI	sucrose	+	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite
43324	16808 ChEBI	2-dehydro-D-gluconate
43324	58143 ChEBI	5-dehydro-D-gluconate
43324	62968 ChEBI	cellulose

Enzymes

@ref	Value	Activity	Ec
43324	acid phosphatase	+	3.1.3.2
43324	alkaline phosphatase	+	3.1.3.1
43324	catalase	+	1.11.1.6
43324	cytochrome oxidase	-	1.9.3.1
43324	esterase (C 4)	+
43324	esterase Lipase (C 8)	+
43324	leucine arylamidase	+	3.4.11.1
43324	naphthol-AS-BI-phosphohydrolase	+
43324	trypsin	+	3.4.21.4

Fatty acid profile

Metadata FA analysis

@ref

43324

Fatty acid	Percentage
C19:0 cyclo ω8c	33.6
C16:0	22.2
C18:1ω7c	15.9
C14:0	12.5
C14:0 2OH	6.6
C16:0 2OH	3.8
C16:0 3OH	2.1
C18:0	1.2

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Host species
43324	midgut of the honey bee (Apis mellifera) in South Korea	South Korea	Republic of Korea	KOR	Asia	Lactobacilli MRS agar
67770	Midgut of a honey bee Apis mellifera						Apis mellifera
67771	From Apis mellifera midgut	Kyung Hee University	Republic of Korea	KOR	Asia

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Database
43324	Bombella apis strain MRM1 16S ribosomal RNA gene, partial sequence	KU534110

GC content

@ref	GC-content (mol%)	Method
43324	59.5	Thermal denaturation, fluorometry
67770	59.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation.	Harer L, Hilgarth M, Ehrmann MA	Microorganisms	10.3390/microorganisms10051058	2022
Phylogeny	Bombella apis sp. nov., an acetic acid bacterium isolated from the midgut of a honey bee.	Yun JH, Lee JY, Hyun DW, Jung MJ, Bae JW	Int J Syst Evol Microbiol	10.1099/ijsem.0.001921	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43324	Ji-Hyun Yun, June-Young Lee, Dong-Wook Hyun, Mi-Ja Jung, Jin-Woo Bae: Bombella apis sp. nov., an acetic acid bacterium isolated from the midgut of a honey bee. IJSEM 67: 2184 - 2188 2017 ( DOI 10.1099/ijsem.0.001921 , PubMed 28671528 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bombella apis (JCM 31623, KCTC 52452, MRM 1)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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