Name: Bombella apis JCM 31623
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140538

Type strain:

Species: Bombella apis

Culture col. no.: JCM 31623, KCTC 52452, MRM 1

Strain history: <- Jin-Woo Bae, Kyung Hee Univ.

NCBI tax ID(s): 1785988 (species)

Section

Bombella apis JCM 31623 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from midgut of the honey bee in South Korea.

colony-forming
Gram-negative
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Acetobacteraceae*
	Genus	*Bombella*
	Species	**Bombella apis**
	Full Scientific Name (LPSN)	Bombella apis Yun et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43324]	Gram stain	negative

[Ref.: #43324]	Cell length	1.0-2.5 µm

[Ref.: #43324]	Cell width	0.5-0.6 µm

[Ref.: #43324]	Cell shape	rod-shaped

[Ref.: #43324]	Motility	no

[Ref.: #43324]	Colony color	creamy beige
[Ref.: #43324]	Colony shape	circular
[Ref.: #43324]	Cultivation medium used	MRS agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

MRS agar

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Culture medium growth

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Culture medium

Mannitol agar

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Culture medium growth

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Culture medium

0,35% acetic acid agar

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Tryptic soy agar

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Culture medium growth

		Temperature
[Ref.: #43324]	optimum	30-37 °C
[Ref.: #67770]	growth	30 °C
[Ref.: #67771]	growth	30-37 °C

[Ref.: #43324]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43324]			optimum	7-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43324]	Oxygen tolerance	obligate aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43324]		NaCl		growth	0	%(w/v)

[Ref.: #43324]	Observation	growth in the presence of 30% (w/v) glucose
[Ref.: #43324]	Observation	ubiquinone Q-10
[Ref.: #67770]	Observation	quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43324]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #43324]	acetate ChEBI	+/-	oxidation
[Ref.: #43324]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #43324]	alpha-D-glucose ChEBI	+	energy source
[Ref.: #43324]	ammonium ChEBI	-	growth
[Ref.: #43324]	ammonium	-	nitrogen source
[Ref.: #43324]	D-arabinose ChEBI	-	builds acid from
[Ref.: #43324]	D-fructose ChEBI	+	builds acid from
[Ref.: #43324]	D-fructose ChEBI	+	carbon source
[Ref.: #43324]	D-fructose ChEBI	+	energy source
[Ref.: #43324]	D-fucose ChEBI	+	builds acid from
[Ref.: #43324]	D-galactose ChEBI	+	builds acid from
[Ref.: #43324]	D-glucose ChEBI	+	builds acid from
[Ref.: #43324]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43324]	D-mannose ChEBI	+	builds acid from
[Ref.: #43324]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43324]	D-xylose ChEBI	+	builds acid from
[Ref.: #43324]	ethanol ChEBI	-	oxidation
[Ref.: #43324]	ethanol ChEBI	-	builds acid from
[Ref.: #43324]	gentiobiose ChEBI	+	builds acid from
[Ref.: #43324]	glycerol ChEBI	+	builds acid from
[Ref.: #43324]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43324]	L-proline ChEBI	+	carbon source
[Ref.: #43324]	L-proline ChEBI	+	energy source
[Ref.: #43324]	lactate ChEBI	-	oxidation
[Ref.: #43324]	melibiose ChEBI	+	builds acid from
[Ref.: #43324]	methanol ChEBI	-	carbon source
[Ref.: #43324]	raffinose ChEBI	-	builds acid from
[Ref.: #43324]	sucrose ChEBI	+	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43324]	2-dehydro-D-gluconate ChEBI	yes
[Ref.: #43324]	5-dehydro-D-gluconate ChEBI	yes
[Ref.: #43324]	cellulose ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43324]	acid phosphatase	+	3.1.3.2
[Ref.: #43324]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43324]	catalase	+	1.11.1.6
[Ref.: #43324]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43324]	esterase (C 4)	+
[Ref.: #43324]	esterase Lipase (C 8)	+
[Ref.: #43324]	leucine arylamidase	+	3.4.11.1
[Ref.: #43324]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43324]	trypsin	+	3.4.21.4

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C19:0 cyclo ω8c	33.6
C16:0	22.2
C18:1ω7c	15.9
C14:0	12.5
C14:0 2OH	6.6
C16:0 2OH	3.8
C16:0 3OH	2.1
C18:0	1.2

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43324]	Sample type/isolated from	midgut of the honey bee (Apis mellifera) in South Korea
[Ref.: #43324]	Geographic location (country and/or sea, region)	South Korea
[Ref.: #43324]	Country	Republic of Korea
[Ref.: #43324]	Country ISO 3 Code	KOR
[Ref.: #43324]	Continent	Asia
[Ref.: #43324]	Enrichment culture	Lactobacilli MRS agar

[Ref.: #67770]	Sample type/isolated from	Midgut of a honey bee Apis mellifera
[Ref.: #67770]	Host species	Apis mellifera

[Ref.: #67771]	Sample type/isolated from	From Apis mellifera midgut
[Ref.: #67771]	Geographic location (country and/or sea, region)	Kyung Hee University
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43324]	16S rRNA gene sequence	KU534110		GenBank

[Ref.: #43324]	GC-content	59.5 mol%	Thermal denaturation, fluorometry
[Ref.: #67770]	GC-content	59.5 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

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Culture collection no.

JCM 31623, KCTC 52452, MRM 1

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation.	Harer L, Hilgarth M, Ehrmann MA	Microorganisms	10.3390/microorganisms10051058	2022	*
Phylogeny	Bombella apis sp. nov., an acetic acid bacterium isolated from the midgut of a honey bee.	Yun JH, Lee JY, Hyun DW, Jung MJ, Bae JW	Int J Syst Evol Microbiol	10.1099/ijsem.0.001921	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43324

Ji-Hyun Yun, June-Young Lee, Dong-Wook Hyun, Mi-Ja Jung, Jin-Woo Bae: Bombella apis sp. nov., an acetic acid bacterium isolated from the midgut of a honey bee. IJSEM 67: 2184 - 2188 2017 ( DOI 10.1099/ijsem.0.001921 , PubMed 28671528 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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