Name: Bacillus spizizenii 2A2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 138329

Type strain:

Species: Bacillus spizizenii

Strain Designation: 2A2

Culture col. no.: CIP 106095, NRRL B-14820, WB 672

Strain history: CIP <- 1999, L.K. Nakamura, NCAUR, Illinois, USA <- BGSC: strain 2A2 <- Nester. E.W.: strain WB672

NCBI tax ID(s): 1423 (species)

SI-ID 13134

LMG 19155, NRRL B-14820, CIP 106095, NBRC 101241

Other strains from Bacillus spizizenii

B. spizizenii TU-B-10, DSM 15029, NRRL B-23049, ... (type strain)
B. spizizenii 231, NRS-231, DSM 347, ATCC 6633, ...
B. spizizenii DSM 618, CIP 107063
B. spizizenii W 23, DSM 1087
B. spizizenii W23 2A2, 2A2, DSM 6395, BGSC 2A2, ...
B. spizizenii SB 623, DSM 6399, BGSC 2A1
B. spizizenii W23 SR, DSM 6405, BGSC 2A3
B. spizizenii W23, DSM 8439, CCM 2268, IAM 12021, ...

Section

Bacillus spizizenii 2A2 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from Prototroph, revertant of BG23, strain W23.

Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus spizizenii**
	Full Scientific Name (LPSN)	Bacillus spizizenii (Nakamura et al. 1999) Dunlap et al. 2020

Synonym

Bacillus subtilis subsp. spizizenii

Information on morphological and physiological properties Morphology

[Ref.: #39464]	Gram stain	positive

[Ref.: #39464]	Cell shape	rod-shaped

[Ref.: #39464]	Motility	yes

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Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

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Culture medium

MEDIUM 3 - Columbia agar

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

CIP Medium 316

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

CIP Medium 72

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

CIP Medium 3

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #39464]		growth	30 °C
[Ref.: #39464]		growth	22-37 °C
[Ref.: #39464]	no	growth	10 °C
[Ref.: #39464]	no	growth	45 °C
[Ref.: #39464]	no	growth	55 °C

[Ref.: #39464]	Temperature range	mesophilic
[Ref.: #39464]	Temperature range	psychrophilic
[Ref.: #39464]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #39464]			growth	6

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

facultative anaerobe

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Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #39464]		NaCl		growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #39464]	citrate ChEBI	-	carbon source
[Ref.: #39464]	esculin ChEBI	+	hydrolysis
[Ref.: #39464]	hippurate ChEBI	-	hydrolysis
[Ref.: #39464]	nitrate ChEBI	+	reduction
[Ref.: #39464]	nitrate ChEBI	+	respiration
[Ref.: #39464]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #39464]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #39464]		acetoin ChEBI	+
[Ref.: #39464]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #39464]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #39464]	amylase	+
[Ref.: #39464]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #39464]	caseinase	+	3.4.21.50
[Ref.: #39464]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #39464]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #39464]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #39464]	gelatinase	+
[Ref.: #39464]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #39464]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #39464]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #39464]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #39464]	oxidase	+
[Ref.: #39464]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #39464]	tween esterase	+
[Ref.: #39464]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #39464]	1533	not determinedn.d.	+	+	+	+	+	-	+	+	+	+	+	-	-	-	-	+	+	+	+	+	+	+	+	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	-	+	-	+	+	-	-	-	-	+	+	-	-	+	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	+	-	-	-	+	+	+	+	+	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #39464]	9245	-	+	+	+	-	-	-	-	-	-	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #39464]	Sample type/isolated from	Prototroph, revertant of BG23, strain W23
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #39464]

Biosafety level

Risk group (French classification)

Availability in culture collections External links

[Ref.: #39464]

Culture collection no.

CIP 106095, NRRL B-14820, WB 672

[Ref.: #95086]

SI-ID 13134

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39464 Collection of Institut Pasteur ; Curators of the CIP; CIP 106095

#68382

Automatically annotated from API zym .

#95086

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13134.1 )

* These data were automatically processed and therefore are not curated

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