Name: Oceanobacillus oncorhynchi subsp. incaldanensis 20AG
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1374

Type strain:

Species: Oceanobacillus oncorhynchi subsp. incaldanensis

Strain Designation: 20AG

Culture col. no.: DSM 16557, ATCC BAA-954, CIP 109235

Strain history: CIP <- 2006, DSMZ <- I. Romano, ICB Istituto Chimica Biomolecolare, Naples, Italy: strain 20AG

NCBI tax ID(s): 272775 (subspecies)

SI-ID 290988

ATCC BAA-954, DSM 16557, CIP 109235, BCRC 17776

Section

Oceanobacillus oncorhynchi subsp. incaldanensis 20AG is an obligate aerobe, moderately halophilic, mesophilic bacterium that forms circular colonies and was isolated from algal mat collected from a sulfurous spring in the Santa Maria Incaldana site.

colony-forming
Gram-positive
motile
rod-shaped
mesophilic
moderately halophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Oceanobacillus*
	Species	**Oceanobacillus oncorhynchi subsp. incaldanensis**
	Full Scientific Name (LPSN)	Oceanobacillus oncorhynchi subsp. incaldanensis Romano et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #23031]	Gram stain	positive
[Ref.: #121420]	Gram stain	positive

[Ref.: #23031]	Cell length	1.2-2.0 µm

[Ref.: #23031]	Cell width	0.5-0.8 µm

[Ref.: #23031]	Cell shape	rod-shaped
[Ref.: #121420]	Cell shape	rod-shaped

[Ref.: #23031]	Motility	yes
[Ref.: #121420]	Motility	yes

[Ref.: #23031]	Flagellum arrangement	peritrichous

[Ref.: #23031]	Colony color	beige
[Ref.: #23031]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6509]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #6509]

Culture medium growth

[Ref.: #6509]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6509]

Culture medium composition

expand / minimize

[Ref.: #37959]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37959]

Culture medium growth

[Ref.: #37959]

Culture medium composition

expand / minimize

[Ref.: #121420]

Culture medium

CIP Medium 72

[Ref.: #121420]

Culture medium growth

[Ref.: #121420]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6509]	growth	30 °C
[Ref.: #23031]	growth	10.0-40.0 °C
[Ref.: #23031]	optimum	37.0 °C
[Ref.: #37959]	growth	37 °C

[Ref.: #6509]	Temperature range	mesophilic
[Ref.: #23031]	Temperature range	mesophilic
[Ref.: #37959]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23031]			optimum	9.0
[Ref.: #23031]			growth	6.5-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23031]	Oxygen tolerance	obligate aerobe
[Ref.: #121420]	Oxygen tolerance	facultative anaerobe

[Ref.: #23031]

Ability of spore formation

[Ref.: #23031]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23031]		NaCl		growth	5.0-20.0	%(w/v)
[Ref.: #23031]		NaCl		optimum	10.0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	casein	-	hydrolysis
[Ref.: #23031]	cellobiose ChEBI	+	carbon source
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-arabinose ChEBI	+	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-fructose ChEBI	+	carbon source
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-galactose ChEBI	+	carbon source
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-glucose ChEBI	+	carbon source
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-mannose ChEBI	+	carbon source
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-ribose ChEBI	+	carbon source
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-sorbose ChEBI	+	carbon source
[Ref.: #68371]	D-tagatose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	D-xylose ChEBI	+	carbon source
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	gelatin ChEBI	-	hydrolysis
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	glycerol ChEBI	+	carbon source
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23031]	lactose ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23031]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	nitrate ChEBI	+	reduction
[Ref.: #121420]	nitrate ChEBI	+	reduction
[Ref.: #121420]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	sodium acetate ChEBI	+	carbon source
[Ref.: #23031]	sodium citrate ChEBI	+	carbon source
[Ref.: #23031]	starch ChEBI	-	hydrolysis
[Ref.: #23031]	sucrose ChEBI	+	carbon source
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23031]	trehalose ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #23031]	ampicillin ChEBI	yes	25	µg
[Ref.: #23031]	bacitracin ChEBI	yes	10	µg
[Ref.: #23031]	chloramphenicol ChEBI	yes	10	µg
[Ref.: #23031]	erythromycin ChEBI	yes	5	µg
[Ref.: #23031]	kanamycin ChEBI	no			yes	30	µg
[Ref.: #23031]	lincomycin ChEBI	yes	15	µg
[Ref.: #23031]	novobiocin ChEBI	yes	30	µg
[Ref.: #23031]	penicillin g ChEBI	yes	2	µg
[Ref.: #23031]	streptomycin ChEBI	yes	25	µg
[Ref.: #23031]	tetracycline ChEBI	no			yes	30	µg
[Ref.: #23031]	tetracycline ChEBI	yes	50	µg
[Ref.: #23031]	vancomycin ChEBI	yes	30	µg
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23031]		indole ChEBI	no
[Ref.: #121420]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #23031]	catalase	+	1.11.1.6
[Ref.: #121420]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #23031]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121420]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #23031]	urease	-	3.5.1.5
[Ref.: #121420]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121420]	11501	not determinedn.d.	+/-	-	-	+	+	+/-	-	-	+	+	+	+	+	-	-	+	+/-	+	+/-	-	+	+	+	+	+	+	+	+	-	+/-	+	+	-	+/-	+	+/-	-	+/-	+	+	-	+	-	-	+/-	-	+/-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121420]	11736	-	-	+	+	-	+	-	-	-	+	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23031]	Sample type/isolated from	algal mat collected from a sulfurous spring in the Santa Maria Incaldana site
[Ref.: #23031]	Geographic location (country and/or sea, region)	Mondragone, Caserta Province, Campania
[Ref.: #23031]	Country	Italy
[Ref.: #23031]	Country ISO 3 Code	ITA
[Ref.: #23031]	Continent	Europe
[Ref.: #23031]	Enrichment culture	enrichment medium
[Ref.: #23031]	Enrichment culture composition	containing (l-1): Na2CO3 , 3.0 g; KCl, 2.0 g; MgSO4.7H2O, 1.0 g; NaCl, 100 g, trisodium citrate, 3.0 g; yeast extract, 10.0 g; MnCl2.4H2O, 0.36 mg; FeSO4 , 50 mg

[Ref.: #6509]	Sample type/isolated from	algal mat
[Ref.: #6509]	Geographic location (country and/or sea, region)	Campania
[Ref.: #6509]	Country	Italy
[Ref.: #6509]	Country ISO 3 Code	ITA
[Ref.: #6509]	Continent	Europe

[Ref.: #121420]	Sample type/isolated from	Algal mat
[Ref.: #121420]	Geographic location (country and/or sea, region)	Mondragone, Campania
[Ref.: #121420]	Country	Italy
[Ref.: #121420]	Country ISO 3 Code	ITA
[Ref.: #121420]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Spring
#Environmental	#Microbial community	#Microbial mat
#Host	#Algae	-
#Condition	#Sulfuric	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ640134 (>99% sequence identity) for Oceanobacillus from Microbeatlas

Aquatic Samples	49
Soil Samples	127
Animal Samples	222
Plant Samples	81
Total Samples	479

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6509]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121420]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6509]	Oceanobacillus oncorhynchi subsp. incaldanensis partial 16S rRNA gene, type strain 20AGT	AJ640134	1513	ENA	272775 tax ID

[Ref.: #23031]

GC-content

40.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #6509]

Culture collection no.

DSM 16557, ATCC BAA-954, CIP 109235

[Ref.: #71025]

SI-ID 290988

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanobacillus gochujangensis sp. nov., isolated from gochujang a traditional Korean fermented food.	Jang SJ, Kim YJ, Lee SH, Park YS, Park JM, Bai DH	J Microbiol	10.1007/s12275-014-4220-z	2014	*
Phylogeny	Oceanobacillus aidingensis sp. nov., a moderately halophilic bacterium.	Liu W, Yang SS	Antonie Van Leeuwenhoek	10.1007/s10482-014-0128-1	2014	*
Phylogeny	Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor.	Yang JY, Huo YY, Xu XW, Meng FX, Wu M, Wang CS	Int J Syst Evol Microbiol	10.1099/ijs.0.016295-0	2009	*
Phylogeny	Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass.	Raats D, Halpern M	Int J Syst Evol Microbiol	10.1099/ijs.0.64502-0	2007	*
Phylogeny	Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi.	Romano I, Lama L, Nicolaus B, Poli A, Gambacorta A, Giordano A	Int J Syst Evol Microbiol	10.1099/ijs.0.63575-0	2006	*

References

#6509

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16557

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23031

Ida Romano,Licia Lama,Barbara Nicolaus,Annarita Poli,Agata Gambacorta,Assunta Giordano: Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi. IJSEM 56: 805 - 810 2006 ( DOI 10.1099/ijs.0.63575-0 , PubMed 16585699 )

#37959 ; Curators of the CIP;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71025

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID290988.1 )

#121420 Collection of Institut Pasteur ; Curators of the CIP; CIP 109235
* These data were automatically processed and therefore are not curated

Change proposal

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