Strain identifier

BacDive ID: 1374

Type strain: Yes

Species: Oceanobacillus oncorhynchi subsp. incaldanensis

Strain Designation: 20AG

Culture col. no.: DSM 16557, ATCC BAA 954, CIP 109235

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General

@ref: 6509

BacDive-ID: 1374

DSM-Number: 16557

keywords: motile, gram-positive, mesophilic, moderately halophilic, obligate aerobe, Bacteria, 16S sequence, rod-shaped, colony-forming

description: Oceanobacillus oncorhynchi subsp. incaldanensis 20AG is an obligate aerobe, moderately halophilic, mesophilic bacterium that forms circular colonies and was isolated from algal mat collected from a sulfurous spring in the Santa Maria Incaldana site.

strain history: <- I. Romano; 20AG

doi: 10.13145/bacdive1374.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Oceanobacillus
  • species: Oceanobacillus oncorhynchi subsp. incaldanensis
  • full scientific name: Oceanobacillus oncorhynchi subsp. incaldanensis Romano et al. 2006

@ref: 6509

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Oceanobacillus

species: Oceanobacillus oncorhynchi subsp. incaldanensis

full scientific name: Oceanobacillus oncorhynchi subsp. incaldanensis Romano et al. 2006

strain designation: 20AG

type strain: yes

Morphology

cell morphology

  • @ref: 23031
  • gram stain: positive
  • cell length: 1.2-2.0 µm
  • cell width: 0.5-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 23031
  • colony color: beige
  • colony shape: circular

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6509CASO AGAR (MERCK 105458) (DSMZ Medium 220), + MnSO4yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf
37959MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
6509positivegrowth30mesophilic
23031positivegrowth10.0-40.0
23031positiveoptimum37.0mesophilic
23031mesophilic
37959positivegrowth37mesophilic

culture pH

@refabilitytypepH
23031positivegrowth6.5-9.5
23031positiveoptimum9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 23031
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 23031
  • spore formation: no

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23031moderately halophilic
23031NaClpositivegrowth5.0-20.0 %(w/v)
23031NaCloptimum10.0 %(w/v)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
23031casein-hydrolysis
23031gelatin-hydrolysis5291
23031starch-hydrolysis28017
23031cellobiose+carbon source17057
23031D-arabinose+carbon source17108
23031D-fructose+carbon source15824
23031D-galactose+carbon source12936
23031D-glucose+carbon source17634
23031lactose+carbon source17716
23031D-mannose+carbon source16024
23031D-ribose+carbon source16988
23031D-sorbose+carbon source17317
23031D-xylose+carbon source65327
23031glycerol+carbon source17754
23031maltose+carbon source17306
23031sodium acetate+carbon source32954
23031sodium citrate+carbon source53258
23031sucrose+carbon source17992
23031trehalose+carbon source27082
23031nitrate+reduction17632

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistantresistance conc.sensitivity conc.
230316104kanamycinyesnoyes30 µg
2303127902tetracyclineyesnoyes30 µg
2303128669bacitracinyesyes10 µg
2303117698chloramphenicolyesyes10 µg
230316472lincomycinyesyes15 µg
2303118208penicillin gyesyes2 µg
2303128971ampicillinyesyes25 µg
2303117076streptomycinyesyes25 µg
2303128368novobiocinyesyes30 µg
2303128001vancomycinyesyes30 µg
2303148923erythromycinyesyes5 µg
2303127902tetracyclineyesyes50 µg

metabolite production

  • @ref: 23031
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
23031catalase+1.11.1.6
23031cytochrome oxidase+1.9.3.1
23031urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture composition
23031algal mat collected from a sulfurous spring in the Santa Maria Incaldana siteMondragone, Caserta Province, CampaniaItalyITAEuropeenrichment mediumcontaining (l-1): Na2CO3 , 3.0 g; KCl, 2.0 g; MgSO4.7H2O, 1.0 g; NaCl, 100 g, trisodium citrate, 3.0 g; yeast extract, 10.0 g; MnCl2.4H2O, 0.36 mg; FeSO4 , 50 mg
6509algal matCampaniaItalyITAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Spring
#Environmental#Microbial community#Microbial mat
#Host#Algae
#Condition#Sulfuric

Safety information

risk assessment

  • @ref: 6509
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6509
  • description: Oceanobacillus oncorhynchi subsp. incaldanensis partial 16S rRNA gene, type strain 20AGT
  • accession: AJ640134
  • length: 1513
  • database: ena
  • NCBI tax ID: 272775

GC content

  • @ref: 23031
  • GC-content: 40.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 6509

culture collection no.: DSM 16557, ATCC BAA 954, CIP 109235

straininfo link

@refpassport
20218http://www.straininfo.net/strains/677444
20218http://www.straininfo.net/strains/677443
20218http://www.straininfo.net/strains/691318

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6509Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16557)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16557
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23031Ida Romano,Licia Lama,Barbara Nicolaus,Annarita Poli,Agata Gambacorta,Assunta Giordano10.1099/ijs.0.63575-0Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchiIJSEM 56: 805-810 2006
37959Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6954