Strain identifier

BacDive ID: 1374

Type strain: Yes

Species: Oceanobacillus oncorhynchi subsp. incaldanensis

Strain Designation: 20AG

Strain history: CIP <- 2006, DSMZ <- I. Romano, ICB Istituto Chimica Biomolecolare, Naples, Italy: strain 20AG

NCBI tax ID(s): 272775 (subspecies)

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General

@ref: 6509

BacDive-ID: 1374

DSM-Number: 16557

keywords: 16S sequence, Bacteria, obligate aerobe, moderately halophilic, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Oceanobacillus oncorhynchi subsp. incaldanensis 20AG is an obligate aerobe, moderately halophilic, mesophilic bacterium that forms circular colonies and was isolated from algal mat collected from a sulfurous spring in the Santa Maria Incaldana site.

NCBI tax id

  • NCBI tax id: 272775
  • Matching level: subspecies

strain history

@refhistory
6509<- I. Romano; 20AG
121420CIP <- 2006, DSMZ <- I. Romano, ICB Istituto Chimica Biomolecolare, Naples, Italy: strain 20AG

doi: 10.13145/bacdive1374.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Oceanobacillus
  • species: Oceanobacillus oncorhynchi subsp. incaldanensis
  • full scientific name: Oceanobacillus oncorhynchi subsp. incaldanensis Romano et al. 2006

@ref: 6509

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Oceanobacillus

species: Oceanobacillus oncorhynchi subsp. incaldanensis

full scientific name: Oceanobacillus oncorhynchi subsp. incaldanensis Romano et al. 2006

strain designation: 20AG

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23031positive1.2-2.0 µm0.5-0.8 µmrod-shapedyesperitrichous
121420positiverod-shapedyes

colony morphology

  • @ref: 23031
  • colony color: beige
  • colony shape: circular

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6509CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
37959MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121420CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6509positivegrowth30mesophilic
23031positivegrowth10.0-40.0
23031positiveoptimum37.0mesophilic
23031mesophilic
37959positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
23031positivegrowth6.5-9.5alkaliphile
23031positiveoptimum9.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23031obligate aerobe
121420facultative anaerobe

spore formation

  • @ref: 23031
  • spore formation: no

halophily

@refhalophily levelsaltgrowthtested relationconcentration
23031moderately halophilic
23031NaClpositivegrowth5.0-20.0 %(w/v)
23031NaCloptimum10.0 %(w/v)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
23031casein-hydrolysis
23031gelatin-hydrolysis5291
23031starch-hydrolysis28017
23031cellobiose+carbon source17057
23031D-arabinose+carbon source17108
23031D-fructose+carbon source15824
23031D-galactose+carbon source12936
23031D-glucose+carbon source17634
23031lactose+carbon source17716
23031D-mannose+carbon source16024
23031D-ribose+carbon source16988
23031D-sorbose+carbon source17317
23031D-xylose+carbon source65327
23031glycerol+carbon source17754
23031maltose+carbon source17306
23031sodium acetate+carbon source32954
23031sodium citrate+carbon source53258
23031sucrose+carbon source17992
23031trehalose+carbon source27082
23031nitrate+reduction17632
68371Potassium 2-ketogluconate-builds acid from
68371L-arabitol-builds acid from18403
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371glycogen-builds acid from28087
68371raffinose+builds acid from16634
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside+builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-mannitol+builds acid from16899
68371galactitol+builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside+builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
121420nitrate+reduction17632
121420nitrite-reduction16301

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistantresistance conc.sensitivity conc.
230316104kanamycinyesnoyes30 µg
2303127902tetracyclineyesnoyes30 µg
2303128669bacitracinyesyes10 µg
2303117698chloramphenicolyesyes10 µg
230316472lincomycinyesyes15 µg
2303118208penicillin gyesyes2 µg
2303128971ampicillinyesyes25 µg
2303117076streptomycinyesyes25 µg
2303128368novobiocinyesyes30 µg
2303128001vancomycinyesyes30 µg
2303148923erythromycinyesyes5 µg
2303127902tetracyclineyesyes50 µg

metabolite production

@refChebi-IDmetaboliteproduction
2303135581indoleno
12142035581indoleno

enzymes

@refvalueactivityec
23031catalase+1.11.1.6
23031cytochrome oxidase+1.9.3.1
23031urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
121420oxidase+
121420catalase+1.11.1.6
121420urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121420--++-+---+++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121420+/---+++/---+++++--++/-++/--++++++++-+/-++-+/-++/--+/-++-+--+/--+/--+/-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture composition
23031algal mat collected from a sulfurous spring in the Santa Maria Incaldana siteMondragone, Caserta Province, CampaniaItalyITAEuropeenrichment mediumcontaining (l-1): Na2CO3 , 3.0 g; KCl, 2.0 g; MgSO4.7H2O, 1.0 g; NaCl, 100 g, trisodium citrate, 3.0 g; yeast extract, 10.0 g; MnCl2.4H2O, 0.36 mg; FeSO4 , 50 mg
6509algal matCampaniaItalyITAEurope
121420Algal matMondragone, CampaniaItalyITAEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Spring
#Environmental#Microbial community#Microbial mat
#Host#Algae
#Condition#Sulfuric

taxonmaps

  • @ref: 69479
  • File name: preview.99_6693.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_498;97_1273;98_1529;99_6693&stattab=map
  • Last taxonomy: Oceanobacillus
  • 16S sequence: AJ640134
  • Sequence Identity:
  • Total samples: 479
  • soil counts: 127
  • aquatic counts: 49
  • animal counts: 222
  • plant counts: 81

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
65091Risk group (German classification)
1214201Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6509
  • description: Oceanobacillus oncorhynchi subsp. incaldanensis partial 16S rRNA gene, type strain 20AGT
  • accession: AJ640134
  • length: 1513
  • database: ena
  • NCBI tax ID: 272775

GC content

  • @ref: 23031
  • GC-content: 40.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 6509

culture collection no.: DSM 16557, ATCC BAA 954, CIP 109235

straininfo link

  • @ref: 71025
  • straininfo: 290988

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16585699Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi.Romano I, Lama L, Nicolaus B, Poli A, Gambacorta A, Giordano AInt J Syst Evol Microbiol10.1099/ijs.0.63575-02006Bacillaceae/*classification/genetics/*isolation & purification, Eukaryota/growth & development/*microbiology, Fresh Water/chemistry/*microbiology, Lipids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/metabolism, Sulfur/*metabolismGenetics
Phylogeny17267960Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass.Raats D, Halpern MInt J Syst Evol Microbiol10.1099/ijs.0.64502-02007Animals, Bacillaceae/*classification/cytology/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Chironomidae/*microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis/chemistry, Genes, rRNA/genetics, Hydrogen-Ion Concentration, Israel, Molecular Sequence Data, Molecular Weight, Movement, Ovum/*microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sodium Chloride, TemperatureGenetics
Phylogeny19946056Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor.Yang JY, Huo YY, Xu XW, Meng FX, Wu M, Wang CSInt J Syst Evol Microbiol10.1099/ijs.0.016295-02009Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Esculin/metabolism, Fatty Acids/analysis, Hydrogen-Ion Concentration, Microscopy, Electron, Transmission, Molecular Sequence Data, Oxidoreductases/metabolism, Phylogeny, Pigments, Biological/biosynthesis, Polysorbates/metabolism, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Sodium Chloride/metabolismMetabolism
Phylogeny24595860Oceanobacillus aidingensis sp. nov., a moderately halophilic bacterium.Liu W, Yang SSAntonie Van Leeuwenhoek10.1007/s10482-014-0128-12014Amino Acids, Diamino/analysis, Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Lakes/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology, TemperatureGenetics
Phylogeny25079955Oceanobacillus gochujangensis sp. nov., isolated from gochujang a traditional Korean fermented food.Jang SJ, Kim YJ, Lee SH, Park YS, Park JM, Bai DHJ Microbiol10.1007/s12275-014-4220-z2014Bacillaceae/classification/*isolation & purification/*physiology/ultrastructure, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal, Fatty Acids/analysis, Fermentation, *Food Microbiology, Hydrogen-Ion Concentration, Lipids/analysis, Microscopy, Electron, Scanning, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Sodium Chloride, TemperatureBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6509Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16557)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16557
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23031Ida Romano,Licia Lama,Barbara Nicolaus,Annarita Poli,Agata Gambacorta,Assunta Giordano10.1099/ijs.0.63575-0Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov., an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchiIJSEM 56: 805-810 200616585699
37959Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6954
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71025Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID290988.1StrainInfo: A central database for resolving microbial strain identifiers
121420Curators of the CIPCollection of Institut Pasteur (CIP 109235)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109235