Name: Oceanobacillus chironomi T3944 D
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1375

Type strain:

Species: Oceanobacillus chironomi

Strain Designation: T3944 D, T3944D

Culture col. no.: DSM 18262, CIP 109536, KCTC 13626, LMG 23627

Strain history: CIP <- 2007, DSMZ <- M. Halpern, Haifa Univ., Tivon, Israel: strain T3944D

NCBI tax ID(s): 381706 (species)

SI-ID 291182

LMG 23627, DSM 18262, CIP 109536

Section

Oceanobacillus chironomi T3944 D is an obligate aerobe, halotolerant, spore-forming bacterium that forms circular colonies and was isolated from chironomid egg mass from a waste-stabilization pond.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
halotolerant
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Oceanobacillus*
	Species	**Oceanobacillus chironomi**
	Full Scientific Name (LPSN)	Oceanobacillus chironomi Raats and Halpern 2007

Information on morphological and physiological properties Morphology

[Ref.: #23009]	Gram stain	positive
[Ref.: #118234]	Gram stain	positive

[Ref.: #23009]	Cell length	1.3-3.0 µm

[Ref.: #23009]	Cell width	0.8-1.0 µm

[Ref.: #23009]	Cell shape	rod-shaped
[Ref.: #118234]	Cell shape	rod-shaped

[Ref.: #23009]	Motility	yes
[Ref.: #118234]	Motility	yes

[Ref.: #23009]	Flagellum arrangement	peritrichous

[Ref.: #23009]	Colony color	cream-beige
[Ref.: #23009]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7464]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #7464]

Culture medium growth

[Ref.: #7464]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7464]

Culture medium composition

expand / minimize

[Ref.: #33306]

Culture medium

Marine agar (MA)

[Ref.: #33306]

Culture medium growth

[Ref.: #33306]

Culture medium composition

expand / minimize

[Ref.: #118234]

Culture medium

CIP Medium 13

[Ref.: #118234]

Culture medium growth

[Ref.: #118234]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7464]	growth	30 °C
[Ref.: #23009]	growth	12.0-46.0 °C
[Ref.: #23009]	optimum	37.0 °C
[Ref.: #33306]	growth	37 °C

[Ref.: #7464]	Temperature range	mesophilic
[Ref.: #23009]	Temperature range	mesophilic
[Ref.: #33306]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23009]			optimum	8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23009]	Oxygen tolerance	obligate aerobe
[Ref.: #118234]	Oxygen tolerance	obligate aerobe

[Ref.: #23009]	Spore description	ellipsoidal,terminal or subterminal,in swollen sporangia
[Ref.: #23009]	Type of spore	endospore
[Ref.: #23009]	Ability of spore formation	yes

[Ref.: #23009]

Halophily / tolerance level

halotolerant

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23009]		NaCl		growth	0.0-11.0	%
[Ref.: #23009]		NaCl		optimum	1.0-3.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23009]	2,3-butanediol ChEBI	+	respiration
[Ref.: #23009]	2-oxoglutarate ChEBI	+	respiration
[Ref.: #23009]	2-oxopentanoate ChEBI	+	respiration
[Ref.: #23009]	adenosine ChEBI	+	respiration
[Ref.: #23009]	alpha-D-glucose ChEBI	+	respiration
[Ref.: #23009]	alpha-lactose ChEBI	+	respiration
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	casein	-	hydrolysis
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	D-fructose ChEBI	+	respiration
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	D-galactose ChEBI	+	respiration
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23009]	D-mannose ChEBI	+	respiration
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23009]	D-ribose ChEBI	+	respiration
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	dextrin ChEBI	+	respiration
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	gelatin ChEBI	+	hydrolysis
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	glycerol ChEBI	+	respiration
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	L-alaninamide ChEBI	+	respiration
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23009]	maltose ChEBI	+	respiration
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23009]	maltotriose ChEBI	+	respiration
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	melibiose ChEBI	+	respiration
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #23009]	N-acetylglucosamine ChEBI	+	respiration
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #118234]	nitrate ChEBI	+	reduction
[Ref.: #23009]	nitrite ChEBI	+	reduction
[Ref.: #118234]	nitrite ChEBI	+	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #23009]	tween 60 ChEBI	-	hydrolysis
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23009]	ampicillin ChEBI	yes
[Ref.: #23009]	bacitracin ChEBI	yes
[Ref.: #23009]	chloramphenicol ChEBI	yes
[Ref.: #23009]	gentamicin ChEBI	yes
[Ref.: #23009]	kanamycin ChEBI	yes
[Ref.: #23009]	neomycin ChEBI	yes
[Ref.: #23009]	novobiocin ChEBI	yes
[Ref.: #23009]	penicillin g ChEBI	yes
[Ref.: #23009]	streptomycin ChEBI	yes
[Ref.: #23009]	tetracycline ChEBI		yes
[Ref.: #23009]	vancomycin ChEBI	yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23009]		indole ChEBI	no
[Ref.: #118234]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #23009]	catalase	+	1.11.1.6
[Ref.: #118234]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #23009]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118234]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118234]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #118234]	11584	not determinedn.d.	+/-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	+	-	-	-	+	-	-	-	-	-	+	+	+	+	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118234]	11938	-	+	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23009]	Sample type/isolated from	chironomid egg mass from a waste-stabilization pond
[Ref.: #23009]	Geographic location (country and/or sea, region)	near Haifa
[Ref.: #23009]	Country	Israel
[Ref.: #23009]	Country ISO 3 Code	ISR
[Ref.: #23009]	Continent	Asia
[Ref.: #23009]	Enrichment culture	LB agar

[Ref.: #7464]	Sample type/isolated from	chironomid egg mass
[Ref.: #7464]	Country	Israel
[Ref.: #7464]	Country ISO 3 Code	ISR
[Ref.: #7464]	Continent	Asia

[Ref.: #118234]	Sample type/isolated from	Egg mass, chironomid
[Ref.: #118234]	Country	Israel
[Ref.: #118234]	Country ISO 3 Code	ISR
[Ref.: #118234]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Pond (small)
#Host	#Arthropoda	#Insecta
#Host Body Product	#Urogenital tract	#Egg

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence DQ298074 (>99% sequence identity) for Oceanobacillus chironomi subclade from Microbeatlas

Aquatic Samples	55
Soil Samples	90
Animal Samples	199
Plant Samples	19
Total Samples	363

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7464]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118234]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7464]	Oceanobacillus chironomi strain T3944D 16S ribosomal RNA gene, partial sequence	DQ298074	1471	ENA	381706 tax ID

[Ref.: #7464]	GC-content	38.1 mol%
[Ref.: #23009]	GC-content	38.1 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #7464]

Culture collection no.

DSM 18262, CIP 109536, KCTC 13626, LMG 23627

[Ref.: #71026]

SI-ID 291182

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanobacillus luteolus sp. nov., isolated from soil.	Wu M, Yang G, Yu Z, Zhuang L, Jin Y, Zhou S	Int J Syst Evol Microbiol	10.1099/ijs.0.057869-0	2014	*
Phylogeny	Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass.	Raats D, Halpern M	Int J Syst Evol Microbiol	10.1099/ijs.0.64502-0	2007	*

References

#7464

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18262

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23009

Dina Raats,Malka Halpern: Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. IJSEM 57: 255 - 259 2007 ( DOI 10.1099/ijs.0.64502-0 , PubMed 17267960 )

#33306 ; Curators of the CIP;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71026

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID291182.1 )

#118234 Collection of Institut Pasteur ; Curators of the CIP; CIP 109536
* These data were automatically processed and therefore are not curated

Change proposal

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