Name: Streptomyces hygroscopicus 325-15
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 136922

Type strain:

Species: Streptomyces hygroscopicus

Strain Designation: 325-15

Culture col. no.: CIP 108010, KCTC 9840, JCM 4427, IFO 16552, NBRC 16552

Strain history: CIP <- 2003, KCTC <- JCM <- KCC S-0427 <- T. Misato: strain 325-15

NCBI tax ID(s): 1912 (species)

SI-ID 64364

JCM 4427, KCTC 9840, CIP 108010, IFO 16552, NBRC 16552

Other strains from Streptomyces hygroscopicus

S. hygroscopicus B-5050, DSM 41526, ATCC 21582, ...
S. hygroscopicus SF-1293, DSM 41527, ATCC 21705, ...
S. hygroscopicus ST007039(HKI), Tü18(10112),
S. hygroscopicus ST003936(HKI),
S. hygroscopicus CIP 106841, IFO 13946, JCM 4683, ...
S. hygroscopicus CIP 107355, JCM 5049, IFM 1236, ...
S. hygroscopicus CIP 106839, IFO 13815, ATCC 21449, ...

Section

Streptomyces hygroscopicus 325-15 is an obligate aerobe, mesophilic, Gram-positive bacterium of the family Streptomycetaceae.

Gram-positive
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces hygroscopicus**
	Full Scientific Name (LPSN)	Streptomyces hygroscopicus (Jensen 1931) Yüntsen et al. 1956 (Approved Lists 1980)

	Synonym	"Actinomyces* hygroscopicus"*
	Synonym	Streptomyces hygroscopicus subsp. sporocinereus
	Synonym	Streptomyces sporocinereus
	Synonym	Streptomyces endus

Information on morphological and physiological properties Morphology

[Ref.: #36676]	Gram stain	positive

[Ref.: #36676]	Cell shape	rod-shaped

[Ref.: #36676]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #36676]

Culture medium

MEDIUM 122 - for Streptomyces

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Culture medium growth

[Ref.: #36676]

Culture medium composition

expand / minimize

[Ref.: #36676]

Culture medium

CIP Medium 122

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Culture medium growth

[Ref.: #36676]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #36676]		growth	30 °C
[Ref.: #36676]		growth	25-41 °C
[Ref.: #36676]	no	growth	10 °C
[Ref.: #36676]	no	growth	45 °C
[Ref.: #67770]		growth	28 °C

[Ref.: #36676]	Temperature range	mesophilic
[Ref.: #36676]	Temperature range	psychrophilic
[Ref.: #36676]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #36676]

Oxygen tolerance

obligate aerobe

[Ref.: #67770]

Name of produced compound

Nigericin

	Salt	Tested relation		Salt conc.
[Ref.: #36676]	NaCl		growth	0	%
[Ref.: #36676]	NaCl	no	growth	2	%
[Ref.: #36676]	NaCl	no	growth	4	%
[Ref.: #36676]	NaCl	no	growth	6	%
[Ref.: #36676]	NaCl	no	growth	8	%
[Ref.: #36676]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #36676]	citrate ChEBI	-	carbon source
[Ref.: #36676]	esculin ChEBI	-	hydrolysis
[Ref.: #36676]	hippurate ChEBI	-	hydrolysis
[Ref.: #36676]	nitrate ChEBI	-	reduction
[Ref.: #36676]	nitrate ChEBI	-	respiration
[Ref.: #36676]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #36676]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite	Production
[Ref.: #67770]	geldanamycin ChEBI	yes
[Ref.: #36676]	indole ChEBI	no
[Ref.: #67770]	nigericin ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #36676]		acetoin ChEBI	-
[Ref.: #36676]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #36676]	amylase	+
[Ref.: #36676]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #36676]	caseinase	-	3.4.21.50
[Ref.: #36676]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #36676]	DNase	+
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #36676]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #36676]	gelatinase	+
[Ref.: #36676]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #36676]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #36676]	oxidase	-
[Ref.: #36676]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #36676]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #36676]	tryptophan deaminase	-
[Ref.: #36676]	tween esterase	-
[Ref.: #36676]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #36676]	2539	not determinedn.d.	+	+	+	+	+	-	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+	-	+	+	+	-	+	-	-	-	-	+	+	+	+	-	-	-	-	-	+	+	-	+	+	-	-	+	+	+	+	+	-	+	+	+	+	+	-	+	+	-	+	-	-	-	-	-	-	-	+	-	+	+	-	+	-	-	-	+	+	-	-	-	+	-	+	-	+	+	+	+	+	+	+	-	+	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #36676]	10818	-	+	-	+	-	+	-	-	-	+	+	+	+	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Peaty soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Bibai City, Hokkaido
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #36676]	Sample type/isolated from	Environment, Peart soil
[Ref.: #36676]	Geographic location (country and/or sea, region)	Bibai, Hokkaido
[Ref.: #36676]	Country	Japan
[Ref.: #36676]	Country ISO 3 Code	JPN
[Ref.: #36676]	Continent	Asia

[Ref.: #36676]	Geographic location (country and/or sea, region)	Bibai city, Hokkaido
[Ref.: #36676]	Country	Japan
[Ref.: #36676]	Country ISO 3 Code	JPN
[Ref.: #36676]	Continent	Asia
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #36676]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Streptomyces hygroscopicus subsp. duamyceticus gene for 16S rRNA, partial sequence, strain: NBRC 16552	AB184724	1473	ENA	285527 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Streptomyces hygroscopicus duamyceticus JCM4427	651716954	draft	JGI IMG/M	285527 tax ID	*
[Ref.: #66792]	Streptomyces hygroscopicus duamyceticus JCM4427	651716955	draft	JGI IMG/M	285527 tax ID	*

Availability in culture collections External links

[Ref.: #36676]

Culture collection no.

CIP 108010, KCTC 9840, JCM 4427, IFO 16552, NBRC 16552

[Ref.: #93936]

SI-ID 64364

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Heterologous expression of a putative K+/H+ antiporter of S. coelicolor A3(2) enhances K+, acidic-pH shock tolerances, and geldanamycin secretion.	Song JY, Seo YB, Hong SK, Chang YK	J Microbiol Biotechnol	10.4014/jmb.1207.07030	2013	*
Metabolism	New geldanamycin analogs from Streptomyces hygroscopicus.	Wu CZ, Jang JH, Ahn JS, Hong YS	J Microbiol Biotechnol	10.4014/jmb.1206.06026	2012	*
Metabolism	Identification of three positive regulators in the geldanamycin PKS gene cluster of Streptomyces hygroscopicus JCM4427.	Kim W, Lee JJ, Paik SG, Hong YS	J Microbiol Biotechnol	10.4014/jmb.1005.05040	2010	*
Metabolism	Enhancement of geldanamycin production by pH shock in batch culture of Streptomyces hygroscopicus subsp. duamyceticus.	Song JY, Kim YJ, Hong YS, Chang YK	J Microbiol Biotechnol	7362	2008	*
Metabolism	Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427.	Shin JC, Na Z, Lee DH, Kim WC, Lee K, Shen YM, Paik SG, Hong YS, Lee JJ	J Microbiol Biotechnol	7436	2008	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36676 Collection of Institut Pasteur ; Curators of the CIP; CIP 108010

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#93936

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID64364.1 )

* These data were automatically processed and therefore are not curated

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