Strain identifier

BacDive ID: 136922

Type strain: No

Species: Streptomyces hygroscopicus

Strain Designation: 325-15

Strain history: CIP <- 2003, KCTC <- JCM <- KCC S-0427 <- T. Misato: strain 325-15

NCBI tax ID(s): 1912 (species)

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General

@ref: 36676

BacDive-ID: 136922

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-positive, rod-shaped

description: Streptomyces hygroscopicus 325-15 is an obligate aerobe, Gram-positive, rod-shaped bacterium of the family Streptomycetaceae.

NCBI tax id

  • NCBI tax id: 1912
  • Matching level: species

strain history

@refhistory
67770KCC S-0427 <-- T. Misato 325-15.
36676CIP <- 2003, KCTC <- JCM <- KCC S-0427 <- T. Misato: strain 325-15

doi: 10.13145/bacdive136922.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces hygroscopicus
  • full scientific name: Streptomyces hygroscopicus (Jensen 1931) Yüntsen et al. 1956 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Actinomyces hygroscopicus
    20215Streptomyces hygroscopicus subsp. sporocinereus
    20215Streptomyces sporocinereus
    20215Streptomyces endus

@ref: 36676

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces hygroscopicus

strain designation: 325-15

type strain: no

Morphology

cell morphology

  • @ref: 36676
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 36676

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
36676MEDIUM 122 - for StreptomycesyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (2.000 g);Starch maize (10.000 g)
36676CIP Medium 122yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=122

culture temp

@refgrowthtypetemperature
36676positivegrowth30
67770positivegrowth28
36676positivegrowth25-41
36676nogrowth10
36676nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 36676
  • oxygen tolerance: obligate aerobe

compound production

  • @ref: 67770
  • compound: Nigericin

halophily

@refsaltgrowthtested relationconcentration
36676NaClpositivegrowth0 %
36676NaClnogrowth2 %
36676NaClnogrowth4 %
36676NaClnogrowth6 %
36676NaClnogrowth8 %
36676NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3667616947citrate-carbon source
366764853esculin-hydrolysis
36676606565hippurate-hydrolysis
3667617632nitrate-reduction
3667616301nitrite-reduction
3667617632nitrate-respiration

antibiotic resistance

  • @ref: 36676
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
677705292geldanamycinyes
677707569nigericinyes
3667635581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3667615688acetoin-
3667617234glucose-

enzymes

@refvalueactivityec
36676oxidase-
36676beta-galactosidase+3.2.1.23
36676gelatinase+
36676amylase+
36676DNase+
36676caseinase-3.4.21.50
36676catalase+1.11.1.6
36676tween esterase-
36676gamma-glutamyltransferase+2.3.2.2
36676lecithinase+
36676lipase-
36676phenylalanine ammonia-lyase-4.3.1.24
36676protease+
36676tryptophan deaminase-
36676urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
36676-+-+-+---++++----+--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
36676+++++-+-+++++++++++++-+++-+----++++-----++-++--+++++-+++++-++-+-------+-++-+---++---+-+-+++++++-++-

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample type
36676Bibai city, HokkaidoJapanJPNAsia
67770Bibai City, HokkaidoJapanJPNAsiaPeaty soil
36676Bibai, HokkaidoJapanJPNAsiaEnvironment, Peart soil

Safety information

risk assessment

  • @ref: 36676
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Streptomyces hygroscopicus subsp. duamyceticus gene for 16S rRNA, partial sequence, strain: NBRC 16552
  • accession: AB184724
  • length: 1473
  • database: nuccore
  • NCBI tax ID: 285527

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces hygroscopicus duamyceticus JCM4427651716954draftimg285527
66792Streptomyces hygroscopicus duamyceticus JCM4427651716955draftimg285527

External links

@ref: 36676

culture collection no.: CIP 108010, KCTC 9840, JCM 4427, IFO 16552, NBRC 16552

straininfo link

  • @ref: 93936
  • straininfo: 64364

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism18600054Characterization of tailoring genes involved in the modification of geldanamycin polyketide in Streptomyces hygroscopicus JCM4427.Shin JC, Na Z, Lee DH, Kim WC, Lee K, Shen YM, Paik SG, Hong YS, Lee JJJ Microbiol Biotechnol74362008Amino Acid Sequence, Bacterial Proteins/*metabolism, Benzoquinones/*metabolism, Cloning, Molecular, Genes, Bacterial, Genetic Complementation Test, Lactams, Macrocyclic/*metabolism, Molecular Sequence Data, *Multigene Family, Mutagenesis, Insertional, Polyketide Synthases/*metabolism, Streptomyces/*genetics/metabolismEnzymology
Metabolism18633288Enhancement of geldanamycin production by pH shock in batch culture of Streptomyces hygroscopicus subsp. duamyceticus.Song JY, Kim YJ, Hong YS, Chang YKJ Microbiol Biotechnol73622008Benzoquinones/*metabolism, Biomass, *Bioreactors, Hydrogen-Ion Concentration, *Industrial Microbiology, Lactams, Macrocyclic/*metabolism, Microbiological Techniques/*methods, Streptomyces/*metabolism, Time FactorsBiotechnology
Metabolism21124051Identification of three positive regulators in the geldanamycin PKS gene cluster of Streptomyces hygroscopicus JCM4427.Kim W, Lee JJ, Paik SG, Hong YSJ Microbiol Biotechnol10.4014/jmb.1005.050402010Amino Acid Sequence, Bacterial Proteins/chemistry/*genetics/metabolism, Benzoquinones/*metabolism, Gene Expression Regulation, Bacterial, *Genes, Regulator, Lactams, Macrocyclic/*metabolism, Molecular Sequence Data, *Multigene Family, Sequence Alignment, Streptomyces/chemistry/*genetics/metabolismGenetics
Metabolism23124337New geldanamycin analogs from Streptomyces hygroscopicus.Wu CZ, Jang JH, Ahn JS, Hong YSJ Microbiol Biotechnol10.4014/jmb.1206.060262012Benzoquinones/chemistry/*metabolism/pharmacology, HSP90 Heat-Shock Proteins/antagonists & inhibitors, Humans, Lactams, Macrocyclic/chemistry/*metabolism/pharmacology, Molecular Structure, Streptomyces/chemistry/*metabolismPathogenicity
Metabolism23412055Heterologous expression of a putative K+/H+ antiporter of S. coelicolor A3(2) enhances K+, acidic-pH shock tolerances, and geldanamycin secretion.Song JY, Seo YB, Hong SK, Chang YKJ Microbiol Biotechnol10.4014/jmb.1207.070302013Acids/*toxicity, Anti-Bacterial Agents/*metabolism, Benzoquinones/*metabolism, Escherichia coli/genetics/metabolism, Gene Expression, Genetic Complementation Test, Lactams, Macrocyclic/*metabolism, Potassium/*toxicity, Potassium-Hydrogen Antiporters/genetics/*metabolism, Streptomyces coelicolor/*enzymology/genetics, *Stress, PhysiologicalEnzymology

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
36676Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108010Collection of Institut Pasteur (CIP 108010)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
93936Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID64364.1