Name: Lentibacillus halophilus PS11-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1362

Type strain:

Species: Lentibacillus halophilus

Strain Designation: PS11-2

Culture col. no.: DSM 19123, CIP 109343, JCM 12149, PCU 240, TISTR 1549, NCIMB 14363

Strain history: CIP <- 2006, JCM <- 2003, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain PS11-2

NCBI tax ID(s): 295065 (species)

SI-ID 297251

JCM 12149, TISTR 1549, CIP 109343, DSM 19123

Other strains from Lentibacillus halophilus

L. halophilus JCM 12150, TISTR 1550

Section

Lentibacillus halophilus PS11-2 is an obligate aerobe, extremely halophilic, spore-forming bacterium that forms circular colonies and was isolated from fish-sauce fermentation.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
extremely halophilic
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Lentibacillus*
	Species	**Lentibacillus halophilus**
	Full Scientific Name (LPSN)	Lentibacillus halophilus Tanasupawat et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #22999]	Gram stain	positive
[Ref.: #119815]	Gram stain	positive

[Ref.: #22999]	Cell length	1.0-3.0 µm

[Ref.: #22999]	Cell width	0.4-0.6 µm

[Ref.: #22999]	Cell shape	rod-shaped
[Ref.: #119815]	Cell shape	rod-shaped

[Ref.: #22999]	Motility	yes
[Ref.: #119815]	Motility	no

[Ref.: #22999]	Colony size	0.1-0.8 mm
[Ref.: #22999]	Colony color	white to cream
[Ref.: #22999]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7922]

Culture medium

HALOBACTERIA MEDIUM (DSMZ Medium 372)

[Ref.: #7922]

Culture medium growth

[Ref.: #7922]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7922]

Culture medium composition

expand / minimize

[Ref.: #37521]

Culture medium

MEDIUM 204 - for Halorubrum, Marinococcus and Natrialba

[Ref.: #37521]

Culture medium growth

[Ref.: #37521]

Culture medium composition

expand / minimize

[Ref.: #119815]

Culture medium

CIP Medium 204

[Ref.: #119815]

Culture medium growth

[Ref.: #119815]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7922]		growth	30 °C
[Ref.: #22999]		growth	15.0-42.0 °C
[Ref.: #22999]	no	growth	10.0 °C
[Ref.: #22999]	no	growth	45.0 °C
[Ref.: #22999]	no	growth	50.0 °C
[Ref.: #22999]		optimum	30.0-37.0 °C
[Ref.: #37521]		growth	37 °C
[Ref.: #67770]		growth	37 °C

[Ref.: #7922]	Temperature range	mesophilic
[Ref.: #22999]	Temperature range	psychrophilic
[Ref.: #22999]	Temperature range	thermophilic
[Ref.: #22999]	Temperature range	mesophilic
[Ref.: #37521]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	Kind of pH		pH
[Ref.: #22999]		optimum	7.0-7.5
[Ref.: #22999]		growth	6.0-8.0
[Ref.: #22999]	no	growth	5.0
[Ref.: #22999]	no	growth	9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22999]

Oxygen tolerance

obligate aerobe

[Ref.: #22999]	Spore description	spherical, terminal,in swollen sporangia
[Ref.: #22999]	Type of spore	endospore
[Ref.: #22999]	Ability of spore formation	yes

[Ref.: #22999]

Halophily / tolerance level

extremely halophilic

	Salt	Tested relation		Salt conc.
[Ref.: #22999]	NaCl		growth	12.0-30.0	%(w/v)
[Ref.: #22999]	NaCl	no	growth	<10.0	%(w/v)
[Ref.: #22999]	NaCl		optimum	20.0-26.0	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22999]	arginine ChEBI	-	hydrolysis
[Ref.: #22999]	casein	-	hydrolysis
[Ref.: #22999]	cellobiose ChEBI	-	builds acid from
[Ref.: #22999]	D-fructose ChEBI	-	builds acid from
[Ref.: #22999]	D-galactose ChEBI	-	builds acid from
[Ref.: #22999]	D-glucose ChEBI	-	builds acid from
[Ref.: #22999]	D-mannitol ChEBI	-	builds acid from
[Ref.: #22999]	D-mannose ChEBI	-	builds acid from
[Ref.: #22999]	D-ribose ChEBI	-	builds acid from
[Ref.: #22999]	D-trehalose ChEBI	-	builds acid from
[Ref.: #22999]	D-xylose ChEBI	-	builds acid from
[Ref.: #22999]	esculin ChEBI	-	hydrolysis
[Ref.: #22999]	gelatin ChEBI	-	hydrolysis
[Ref.: #22999]	glycerol ChEBI	-	builds acid from
[Ref.: #22999]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #22999]	L-arabinose ChEBI	-	builds acid from
[Ref.: #22999]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #22999]	lactose ChEBI	-	builds acid from
[Ref.: #22999]	maltose ChEBI	-	builds acid from
[Ref.: #22999]	melezitose ChEBI	-	builds acid from
[Ref.: #22999]	melibiose ChEBI	-	builds acid from
[Ref.: #22999]	myo-inositol ChEBI	-	builds acid from
[Ref.: #119815]	nitrate ChEBI	+	reduction
[Ref.: #119815]	nitrite ChEBI	-	reduction
[Ref.: #22999]	phenylalanine ChEBI	-	hydrolysis
[Ref.: #22999]	raffinose ChEBI	-	builds acid from
[Ref.: #22999]	salicin ChEBI	-	builds acid from
[Ref.: #22999]	sorbitol ChEBI	-	builds acid from
[Ref.: #22999]	starch ChEBI	-	hydrolysis
[Ref.: #22999]	sucrose ChEBI	-	builds acid from
[Ref.: #22999]	tween 80 ChEBI	-	hydrolysis
[Ref.: #22999]	tyrosine ChEBI	-	hydrolysis
[Ref.: #22999]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119815]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #22999]	catalase	+	1.11.1.6
[Ref.: #119815]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #22999]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119815]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #22999]	urease	-	3.5.1.5
[Ref.: #119815]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119815]	11801	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22999]	Sample type/isolated from	fish-sauce fermentation
[Ref.: #22999]	Country	Thailand
[Ref.: #22999]	Country ISO 3 Code	THA
[Ref.: #22999]	Continent	Asia
[Ref.: #22999]	Enrichment culture	agar plates of JCM medium no.168
[Ref.: #22999]	Enrichment culture duration	7 days
[Ref.: #22999]	Enrichment culture temperature	37.0 ̊C

[Ref.: #7922]	Sample type/isolated from	fish sauce
[Ref.: #7922]	Country	Thailand
[Ref.: #7922]	Country ISO 3 Code	THA
[Ref.: #7922]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Fish-sauce fermentation in Thailand

[Ref.: #119815]	Sample type/isolated from	Food, Fish, sauce
[Ref.: #119815]	Country	Thailand
[Ref.: #119815]	Country ISO 3 Code	THA
[Ref.: #119815]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Seafood
#Engineered	#Food production	#Fermented

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB191345 (>99% sequence identity) for Lentibacillus halophilus subclade from Microbeatlas

Aquatic Samples	1
Soil Samples	1
Animal Samples	2
Plant Samples
Total Samples	4

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7922]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119815]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7922]	Lentibacillus halophilus gene for 16S rRNA	AB191345	1410	ENA	295065 tax ID

[Ref.: #7922]	GC-content	42.4 mol%
[Ref.: #22999]	GC-content	42.4 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #7922]

Culture collection no.

DSM 19123, CIP 109343, JCM 12149, PCU 240, TISTR 1549, NCIMB 14363

[Ref.: #71013]

SI-ID 297251

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).	Booncharoen A, Visessanguan W, Kuncharoen N, Yiamsombut S, Santiyanont P, Mhuantong W, Charoensri S, Rojsitthisak P, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.003658	2019	*
Phylogeny	Lentibacillus halophilus sp. nov., from fish sauce in Thailand.	Tanasupawat S, Pakdeeto A, Namwong S, Thawai C, Kudo T, Itoh T	Int J Syst Evol Microbiol	10.1099/ijs.0.63997-0	2006	*

References

#7922

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19123

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22999

Somboon Tanasupawat,Amnat Pakdeeto,Sirilak Namwong,Chitti Thawai,Takuji Kudo,Takashi Itoh: Lentibacillus halophilus sp. nov., from fish sauce in Thailand. IJSEM 56: 1859 - 1863 2006 ( DOI 10.1099/ijs.0.63997-0 , PubMed 16902021 )

#37521 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71013

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297251.1 )

#119815 Collection of Institut Pasteur ; Curators of the CIP; CIP 109343
* These data were automatically processed and therefore are not curated

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