Strain identifier

BacDive ID: 1362

Type strain: Yes

Species: Lentibacillus halophilus

Strain Designation: PS11-2

Strain history: CIP <- 2006, JCM <- 2003, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain PS11-2

NCBI tax ID(s): 295065 (species)

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General

@ref: 7922

BacDive-ID: 1362

DSM-Number: 19123

keywords: 16S sequence, Bacteria, obligate aerobe, extremely halophilic, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Lentibacillus halophilus PS11-2 is an obligate aerobe, extremely halophilic, spore-forming bacterium that forms circular colonies and was isolated from fish-sauce fermentation.

NCBI tax id

  • NCBI tax id: 295065
  • Matching level: species

strain history

@refhistory
7922<- JCM <- S. Tanasupawat, PS11-2
67770S. Tanasupawat PS11-2.
119815CIP <- 2006, JCM <- 2003, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain PS11-2

doi: 10.13145/bacdive1362.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lentibacillus
  • species: Lentibacillus halophilus
  • full scientific name: Lentibacillus halophilus Tanasupawat et al. 2006

@ref: 7922

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Lentibacillus

species: Lentibacillus halophilus

full scientific name: Lentibacillus halophilus Tanasupawat et al. 2006

strain designation: PS11-2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
22999positive1.0-3.0 µm0.4-0.6 µmrod-shapedyes
119815positiverod-shapedno

colony morphology

  • @ref: 22999
  • colony size: 0.1-0.8 mm
  • colony color: white to cream
  • colony shape: circular

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7922HALOBACTERIA MEDIUM (DSMZ Medium 372)yeshttps://mediadive.dsmz.de/medium/372Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
37521MEDIUM 204 - for Halorubrum, Marinococcus and NatrialbayesDistilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate
119815CIP Medium 204yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=204

culture temp

@refgrowthtypetemperature
7922positivegrowth30
22999positivegrowth15.0-42.0
22999nogrowth10.0
22999nogrowth45.0
22999nogrowth50.0
22999positiveoptimum30.0-37.0
37521positivegrowth37
67770positivegrowth37

culture pH

@refabilitytypepH
22999positivegrowth6.0-8.0
22999positiveoptimum7.0-7.5
22999nogrowth5.0
22999nogrowth9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 22999
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 22999
  • spore description: spherical, terminal,in swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refhalophily levelsaltgrowthtested relationconcentration
22999extremely halophilicNaClpositivegrowth12.0-30.0 %(w/v)
22999NaClnogrowth<10.0 %(w/v)
22999NaCloptimum20.0-26.0 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2299917057cellobiose-builds acid from
2299915824D-fructose-builds acid from
2299912936D-galactose-builds acid from
2299917634D-glucose-builds acid from
2299916899D-mannitol-builds acid from
2299916024D-mannose-builds acid from
229996731melezitose-builds acid from
2299928053melibiose-builds acid from
2299916988D-ribose-builds acid from
2299916551D-trehalose-builds acid from
2299965327D-xylose-builds acid from
2299917754glycerol-builds acid from
2299930849L-arabinose-builds acid from
2299962345L-rhamnose-builds acid from
2299917716lactose-builds acid from
2299917306maltose-builds acid from
2299917268myo-inositol-builds acid from
2299916634raffinose-builds acid from
2299917814salicin-builds acid from
2299930911sorbitol-builds acid from
2299917992sucrose-builds acid from
2299929016arginine-hydrolysis
22999casein-hydrolysis
229994853esculin-hydrolysis
229995291gelatin-hydrolysis
2299917368hypoxanthine-hydrolysis
2299928044phenylalanine-hydrolysis
2299928017starch-hydrolysis
2299953426tween 80-hydrolysis
2299918186tyrosine-hydrolysis
2299915318xanthine-hydrolysis
11981517632nitrate+reduction
11981516301nitrite-reduction

metabolite production

  • @ref: 119815
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
22999catalase+1.11.1.6
22999cytochrome oxidase+1.9.3.1
22999urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
119815oxidase+
119815catalase+1.11.1.6
119815urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119815--+--+-----+--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
22999fish-sauce fermentationThailandTHAAsiaagar plates of JCM medium no.1687 days37.0
7922fish sauceThailandTHAAsia
67770Fish-sauce fermentation in Thailand
119815Food, Fish, sauceThailandTHAAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Seafood
#Engineered#Food production#Fermented

taxonmaps

  • @ref: 69479
  • File name: preview.99_39827.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_13854;97_16868;98_29103;99_39827&stattab=map
  • Last taxonomy: Lentibacillus halophilus subclade
  • 16S sequence: AB191345
  • Sequence Identity:
  • Total samples: 4
  • soil counts: 1
  • aquatic counts: 1
  • animal counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
79221Risk group (German classification)
1198151Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7922
  • description: Lentibacillus halophilus gene for 16S rRNA
  • accession: AB191345
  • length: 1410
  • database: nuccore
  • NCBI tax ID: 295065

GC content

@refGC-contentmethod
792242.4
2299942.4high performance liquid chromatography (HPLC)

External links

@ref: 7922

culture collection no.: DSM 19123, CIP 109343, JCM 12149, PCU 240, TISTR 1549, NCIMB 14363

straininfo link

  • @ref: 71013
  • straininfo: 297251

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny16902021Lentibacillus halophilus sp. nov., from fish sauce in Thailand.Tanasupawat S, Pakdeeto A, Namwong S, Thawai C, Kudo T, Itoh TInt J Syst Evol Microbiol10.1099/ijs.0.63997-02006Animals, Bacillaceae/chemistry/*classification/genetics/growth & development/isolation & purification, Culture Media, Cyanoacrylates/analysis, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Fermentation, Fish Products/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride, Species Specificity, Thailand, Vitamin K 2/analogs & derivativesCultivation
Phylogeny31424384Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).Booncharoen A, Visessanguan W, Kuncharoen N, Yiamsombut S, Santiyanont P, Mhuantong W, Charoensri S, Rojsitthisak P, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0036582019Animals, Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Crustacea/microbiology, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Food Microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood/*microbiology, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryBiotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7922Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19123)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19123
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
22999Somboon Tanasupawat,Amnat Pakdeeto,Sirilak Namwong,Chitti Thawai,Takuji Kudo,Takashi Itoh10.1099/ijs.0.63997-0Lentibacillus halophilus sp. nov., from fish sauce in ThailandIJSEM 56: 1859-1863 200616902021
37521Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7074
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71013Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297251.1StrainInfo: A central database for resolving microbial strain identifiers
119815Curators of the CIPCollection of Institut Pasteur (CIP 109343)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109343