Name: Lentibacillus lacisalsi BH260
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1361

Type strain:

Species: Lentibacillus lacisalsi

Strain Designation: BH260

Culture col. no.: DSM 16462, KCTC 3915

Strain history: <- C.-J. Kim <- J.-M. Lim <- D.-J. Park; BH260

NCBI tax ID(s): 284575 (species)

SI-ID 232135

KCTC 3915, DSM 16462

Special Collections

Section

Lentibacillus lacisalsi BH260 is an obligate aerobe, moderately halophilic, spore-forming bacterium that forms circular colonies and was isolated from soil sediment of a salt lake.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
moderately halophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Lentibacillus*
	Species	**Lentibacillus lacisalsi**
	Full Scientific Name (LPSN)	Lentibacillus lacisalsi Lim et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #22998]	Gram stain	positive

[Ref.: #22998]	Cell length	1.2-3.0 µm

[Ref.: #22998]	Cell width	0.4-0.6 µm

[Ref.: #22998]	Cell shape	rod-shaped

[Ref.: #22998]	Motility	yes

[Ref.: #22998]	Flagellum arrangement	peritrichous

[Ref.: #22998]	Colony color	cream
[Ref.: #22998]	Colony shape	circular
[Ref.: #22998]	Cultivation medium used	MA supplemented with 10 % (w/v) NaCl

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6453]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #6453]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #6453]

Culture medium composition

expand / minimize

[Ref.: #22998]

Culture medium

MA supplemented with 10 % (w/v) NaCl

[Ref.: #22998]

Culture medium growth

		Temperature
[Ref.: #6453]	growth	30 °C
[Ref.: #22998]	growth	15.0-40.0 °C
[Ref.: #22998]	optimum	30.0-32.0 °C

	pH	Kind of pH		pH
[Ref.: #22998]			optimum	8.0
[Ref.: #22998]			growth	7.0-9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22998]

Oxygen tolerance

obligate aerobe

[Ref.: #22998]	Spore description	spherical,terminal,in swollen sporangia
[Ref.: #22998]	Type of spore	endospore
[Ref.: #22998]	Ability of spore formation	yes

[Ref.: #22998]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #22998]		NaCl		growth	5.0-25.0	%(w/v)
[Ref.: #22998]		NaCl		optimum	12.0-15.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22998]	alpha-lactose ChEBI	-	builds acid from
[Ref.: #22998]	arbutin ChEBI	-	builds acid from
[Ref.: #22998]	casein	-	hydrolysis
[Ref.: #22998]	D-arabinose ChEBI	+	builds acid from
[Ref.: #22998]	D-fructose ChEBI	+	builds acid from
[Ref.: #22998]	D-glucose ChEBI	-	builds acid from
[Ref.: #22998]	D-mannitol ChEBI	-	builds acid from
[Ref.: #22998]	D-mannose ChEBI	-	builds acid from
[Ref.: #22998]	D-ribose ChEBI	+	builds acid from
[Ref.: #22998]	D-trehalose ChEBI	-	builds acid from
[Ref.: #22998]	D-xylose ChEBI	+	builds acid from
[Ref.: #22998]	esculin ChEBI	-	hydrolysis
[Ref.: #22998]	glycerol ChEBI	-	builds acid from
[Ref.: #22998]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #22998]	L-arabinose ChEBI	+	builds acid from
[Ref.: #22998]	L-tyrosine ChEBI	-	hydrolysis
[Ref.: #22998]	maltose ChEBI	-	builds acid from
[Ref.: #22998]	melibiose ChEBI	-	builds acid from
[Ref.: #22998]	nitrate ChEBI	+	reduction
[Ref.: #22998]	raffinose ChEBI	-	builds acid from
[Ref.: #22998]	rhamnose ChEBI	-	builds acid from
[Ref.: #22998]	ribitol ChEBI	-	builds acid from
[Ref.: #22998]	salicin ChEBI	-	builds acid from
[Ref.: #22998]	starch ChEBI	-	hydrolysis
[Ref.: #22998]	tween 80 ChEBI	-	hydrolysis
[Ref.: #22998]	urea ChEBI	-	hydrolysis
[Ref.: #22998]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #22998]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22998]	Sample type/isolated from	soil sediment of a salt lake
[Ref.: #22998]	Geographic location (country and/or sea, region)	Xin-Jiang Province
[Ref.: #22998]	Country	China
[Ref.: #22998]	Country ISO 3 Code	CHN
[Ref.: #22998]	Continent	Asia
[Ref.: #22998]	Enrichment culture	marine agar 2216 (MA;Difco) with the addition of 20 % (w/v) NaCl [final concentration, 21.94 % (w/v) NaCl]
[Ref.: #22998]	Enrichment culture duration	3 days
[Ref.: #22998]	Enrichment culture temperature	28.0 ̊C

[Ref.: #6453]	Sample type/isolated from	salt lake soil
[Ref.: #6453]	Country	China
[Ref.: #6453]	Country ISO 3 Code	CHN
[Ref.: #6453]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Environmental	#Terrestrial	#Sediment
#Environmental	#Terrestrial	#Soil
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY667497 (>99% sequence identity) for Lentibacillus lacisalsi subclade from Microbeatlas

Aquatic Samples	2
Soil Samples	11
Animal Samples	12
Plant Samples	1
Total Samples	26

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6453]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #22998]	Lentibacillus lacisalsi strain BH260 16S ribosomal RNA gene, partial sequence	AY667497	1365	GenBank ENA	284575 tax ID

[Ref.: #6453]	GC-content	44 mol%
[Ref.: #22998]	GC-content	44 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #6453]

Culture collection no.

DSM 16462, KCTC 3915

[Ref.: #71012]

SI-ID 232135

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lentibacillus salis sp. nov., a moderately halophilic bacterium isolated from a salt lake.	Lee JC, Li WJ, Xu LH, Jiang CL, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.65545-0	2008	*
Phylogeny	Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China.	Lim JM, Jeon CO, Song SM, Lee JC, Ju YJ, Xu LH, Jiang CL, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.63721-0	2005	*

References

#6453

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16462

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22998

Jee-Min Lim,Che Ok Jeon,Sung-Min Song,Jae-Chan Lee,Yoon Jung Ju,Li-Hua Xu,Cheng-Lin Jiang,Chang-Jin Kim: Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China. IJSEM 55: 1805 - 1809 2005 ( DOI 10.1099/ijs.0.63721-0 , PubMed 16166670 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71012

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID232135.1 )

* These data were automatically processed and therefore are not curated

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