Name: Halobacillus kuroshimensis JS-Hb7
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1349

Type strain:

Species: Halobacillus kuroshimensis

Strain Designation: JS-Hb7, IS-Hb7

Culture col. no.: DSM 18393, JCM 14155, CIP 109650

Strain history: CIP <- 2007, JCM <- T. Naganuma, Hiroshima Univ., Hiroshima, Japan: strain IS-Hb7

NCBI tax ID(s): 1121938 (strain), 302481 (species)

SI-ID 309457

DSM 18393, JCM 14155, CIP 109650

Special Collections

Section

Halobacillus kuroshimensis JS-Hb7 is a spore-forming, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from carbonate rock collected from a 642 m-deep methane cold seep.

colony-forming
Gram-positive
rod-shaped
spore-forming
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halobacillus*
	Species	**Halobacillus kuroshimensis**
	Full Scientific Name (LPSN)	Halobacillus kuroshimensis Hua et al. 2007

Information on morphological and physiological properties Morphology

[Ref.: #22985]	Gram stain	positive
[Ref.: #120515]	Gram stain	positive

[Ref.: #22985]	Cell length	1.6-3.3 µm

[Ref.: #22985]	Cell width	0.5-1.0 µm

[Ref.: #22985]	Cell shape	rod-shaped
[Ref.: #120515]	Cell shape	ovoid-shaped

[Ref.: #22985]	Motility	no
[Ref.: #120515]	Motility	no

[Ref.: #22985]	Colony size	1.5-2.5 mm
[Ref.: #22985]	Colony color	yellow-orange
[Ref.: #22985]	Colony shape	circular
[Ref.: #22985]	Incubation period	3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7522]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #7522]

Culture medium growth

[Ref.: #7522]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7522]

Culture medium composition

expand / minimize

[Ref.: #37807]

Culture medium

Marine agar (MA)

[Ref.: #37807]

Culture medium growth

[Ref.: #37807]

Culture medium composition

expand / minimize

[Ref.: #120515]

Culture medium

CIP Medium 13

[Ref.: #120515]

Culture medium growth

[Ref.: #120515]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7522]	growth	30 °C
[Ref.: #22985]	growth	9.0-48.0 °C
[Ref.: #22985]	optimum	25.0 °C
[Ref.: #37807]	growth	30 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #22985]			optimum	7.5-8.0
[Ref.: #22985]			growth	5.5-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22985]	Spore description	spherical or ellipsoidal, terminal position
[Ref.: #22985]	Type of spore	endospore
[Ref.: #22985]	Ability of spore formation	yes
[Ref.: #120515]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	yes Confidence in %93.448
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #22985]		NaCl		growth	0.5-25.0	%(w/v)
[Ref.: #22985]		NaCl		optimum	6.0	%

[Ref.: #7522]	Murein short key	A21.04
[Ref.: #7522]	Murein types	A4ß L-Orn-D-Asp

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22985]	alpha-cyclodextrin ChEBI	-	hydrolysis
[Ref.: #22985]	alpha-D-glucose ChEBI	+	carbon source
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	casein	+	hydrolysis
[Ref.: #22985]	cellobiose ChEBI	+	builds acid from
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	D-fructose ChEBI	+	builds acid from
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	D-galactose ChEBI	-	builds acid from
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22985]	D-glucose ChEBI	+	builds acid from
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	D-mannitol ChEBI	-	builds acid from
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22985]	D-mannose ChEBI	+	carbon source
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	D-tagatose ChEBI	-	builds acid from
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	D-trehalose ChEBI	-	builds acid from
[Ref.: #22985]	D-xylose ChEBI	-	builds acid from
[Ref.: #22985]	D-xylose ChEBI	+	carbon source
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	dextrin ChEBI	-	hydrolysis
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	gelatin ChEBI	+	hydrolysis
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	glycerol ChEBI	+	builds acid from
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22985]	glycogen ChEBI	-	hydrolysis
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	maltose ChEBI	+	builds acid from
[Ref.: #22985]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22985]	mannose ChEBI	+	builds acid from
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	melibiose ChEBI	-	builds acid from
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	myo-inositol ChEBI	+	builds acid from
[Ref.: #22985]	myo-inositol ChEBI	+	carbon source
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22985]	nitrate ChEBI	-	reduction
[Ref.: #120515]	nitrate ChEBI	-	reduction
[Ref.: #120515]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	salicin ChEBI	-	builds acid from
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22985]	starch ChEBI	+	hydrolysis
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22985]	sucrose ChEBI	+	builds acid from
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22985]	tween 20 ChEBI	+	hydrolysis
[Ref.: #22985]	tween 40 ChEBI	+	carbon source
[Ref.: #22985]	tween 40 ChEBI	+	hydrolysis
[Ref.: #22985]	tween 80 ChEBI	+	carbon source
[Ref.: #22985]	tween 80 ChEBI	+	hydrolysis
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120515]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #22985]	catalase	+	1.11.1.6
[Ref.: #120515]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #22985]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120515]	oxidase	+
[Ref.: #22985]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #22985]	urease	-	3.5.1.5
[Ref.: #120515]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120515]	11625	not determinedn.d.	+	-	-	-	+	-	-	-	-	+	+	+	+	-	+/-	-	-	+	-	-	-	+	-	-	-	-	+	+	-	-	+	+	-	-	+	+	+	-	-	+	-	-	-	-	+/-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120515]	12030	-	+	+	+	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #22985]	Sample type/isolated from	carbonate rock collected from a 642 m-deep methane cold seep
[Ref.: #22985]	Geographic location (country and/or sea, region)	Kuroshima Knoll
[Ref.: #22985]	Country	Japan
[Ref.: #22985]	Country ISO 3 Code	JPN
[Ref.: #22985]	Continent	Asia
[Ref.: #22985]	Geographic location	24.1300°/124.1870°
[Ref.: #22985]	Enrichment culture	enrichment medium containing 0.5 % (w/v) Bacto-peptone (Difco),0.25 % yeast extract (Difco),0.1 % D-glucose (Wako) and 15 % NaCl

[Ref.: #7522]	Sample type/isolated from	deep-sea carbonate rock
[Ref.: #7522]	Geographic location (country and/or sea, region)	Kuroshima Knoll
[Ref.: #7522]	Country	Japan
[Ref.: #7522]	Country ISO 3 Code	JPN
[Ref.: #7522]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #120515]	Sample type/isolated from	Environment, Deep sea carbonate rock
[Ref.: #120515]	Geographic location (country and/or sea, region)	Carbonate rocks authentically formed in a methane seep of the Kuroshima Knoll, located at 642 m depth (Fujikuraet al., 2003, Inagakiet al., 2004), were collected with the remotely operated vehicle Dolphin 3K, operated by the Japan Agency for Marine Earth Science and Technology (JAMSTEC).
[Ref.: #120515]	Country	Japan
[Ref.: #120515]	Country ISO 3 Code	JPN
[Ref.: #120515]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Geologic
#Condition	#Psychrophilic (<10°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7522]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120515]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7522]	Halobacillus kuroshimensis gene for 16S rRNA, partial sequence	AB195680	1544	GenBank ENA	302481 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Halobacillus kuroshimensis DSM 18393 DSM 18393	GCA_000425705	scaffold	GenBank ENA	1121938 tax ID
[Ref.: #66792]	Halobacillus kuroshimensis DSM 18393	1121938.3	wgs	PATRIC	1121938 tax ID	*
[Ref.: #66792]	Halobacillus kuroshimensis DSM 18393	2523533634	draft	JGI IMG/M	1121938 tax ID	*

[Ref.: #7522]	GC-content	42.1 mol%
[Ref.: #67770]	GC-content	42.1 mol%	capillary zone electrophoresis (CZE)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Halobacillus kuroshimensis DSM 18393 DSM 18393 NCBI: GCA_000425705.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halobacillus kuroshimensis DSM 18393 PATRIC: 1121938.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	81.931	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.86	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	93.448	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	76.568	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.268	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.704	no

Availability in culture collections External links

[Ref.: #7522]

Culture collection no.

DSM 18393, JCM 14155, CIP 109650

[Ref.: #70999]

SI-ID 309457

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep.	Hua NP, Kanekiyo A, Fujikura K, Yasuda H, Naganuma T	Int J Syst Evol Microbiol	10.1099/ijs.0.64817-0	2007	*

References

#7522

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18393

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#22985

Ngoc-Phuc Hua,Atsuko Kanekiyo,Katsunori Fujikura,Hisato Yasuda,Takeshi Naganuma: Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. IJSEM 57: 1243 - 1249 2007 ( DOI 10.1099/ijs.0.64817-0 , PubMed 17551037 )

#37807 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70999

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID309457.1 )

#120515 Collection of Institut Pasteur ; Curators of the CIP; CIP 109650
* These data were automatically processed and therefore are not curated

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Halobacillus kuroshimensis DSM 18393 DSM 18393

Halobacillus kuroshimensis DSM 18393

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Change proposal