Name: Halobacillus dabanensis D-8
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1348

Type strain:

Species: Halobacillus dabanensis

Strain Designation: D-8

Culture col. no.: DSM 18199, CGMCC 1.3704, JCM 12772, CIP 109012

Strain history: CIP <- 2005, JCM <- 2004, W.Y. Liu, China Agric. Univ., Beijing, China

NCBI tax ID(s): 240302 (species)

SI-ID 265604

CGMCC 1.3704, JCM 12772, DSM 18199, CIP 109012

Special Collections

Section

Halobacillus dabanensis D-8 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and has a orange pigmentation.

colony-forming
Gram-positive
motile
pigmented
rod-shaped
spore-forming
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halobacillus*
	Species	**Halobacillus dabanensis**
	Full Scientific Name (LPSN)	Halobacillus dabanensis Liu et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #22987]	Gram stain	positive
[Ref.: #118838]	Gram stain	positive

[Ref.: #22987]	Cell length	2.2-4.2 µm

[Ref.: #22987]	Cell width	0.6-0.9 µm

[Ref.: #22987]	Cell shape	rod-shaped
[Ref.: #118838]	Cell shape	rod-shaped

[Ref.: #22987]	Motility	yes
[Ref.: #118838]	Motility	yes

[Ref.: #22987]	Flagellum arrangement	peritrichous

[Ref.: #22987]	Colony size	3.0-4.0 mm
[Ref.: #22987]	Colony color	cream
[Ref.: #22987]	Colony shape	circular
[Ref.: #22987]	Incubation period	3 days

[Ref.: #22987]	Pigment production	yes
[Ref.: #22987]	Pigmentation color	orange

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7423]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #7423]

Culture medium growth

[Ref.: #7423]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7423]

Culture medium composition

expand / minimize

[Ref.: #40089]

Culture medium

MEDIUM 665 -for Halobacillus aidigensis

[Ref.: #40089]

Culture medium growth

[Ref.: #40089]

Culture medium composition

expand / minimize

[Ref.: #118838]

Culture medium

CIP Medium 413

[Ref.: #118838]

Culture medium growth

[Ref.: #118838]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7423]	growth	30 °C
[Ref.: #22987]	growth	15.0-50.0 °C
[Ref.: #22987]	optimum	35.0 °C
[Ref.: #40089]	growth	30 °C
[Ref.: #67770]	growth	35 °C

	pH	Kind of pH		pH
[Ref.: #22987]			optimum	7.5
[Ref.: #22987]			growth	5.0-11.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118838]

Oxygen tolerance

facultative anaerobe

[Ref.: #22987]	Spore description	ellipsoidal,central or subterminal position
[Ref.: #22987]	Type of spore	endospore
[Ref.: #22987]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %93.75
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #22987]		NaCl		growth	0.5-25.0	%(w/v)
[Ref.: #22987]		NaCl		optimum	10.0	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	casein	+	hydrolysis
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	D-fructose ChEBI	+	builds acid from
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	D-galactose ChEBI	-	builds acid from
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	D-glucose ChEBI	+	builds acid from
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	D-mannitol ChEBI	+	builds acid from
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	D-trehalose ChEBI	+	builds acid from
[Ref.: #22987]	D-xylose ChEBI	+	builds acid from
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	gelatin ChEBI	-	hydrolysis
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	maltose ChEBI	+	builds acid from
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22987]	nitrate ChEBI	-	reduction
[Ref.: #118838]	nitrate ChEBI	-	reduction
[Ref.: #118838]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22987]	starch ChEBI	+	hydrolysis
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #22987]	sucrose ChEBI	+	builds acid from
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #22987]	tween 80 ChEBI	-	hydrolysis
[Ref.: #22987]	tyrosine ChEBI	-	hydrolysis
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #22987]		acetoin ChEBI	no
[Ref.: #118838]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #22987]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118838]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #22987]	catalase	+	1.11.1.6
[Ref.: #118838]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #22987]	cytochrome oxidase	+	1.9.3.1
[Ref.: #22987]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118838]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #118838]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #118838]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #22987]	urease	-	3.5.1.5
[Ref.: #118838]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #118838]	11449	not determinedn.d.	+	-	-	-	+/-	+/-	-	-	+/-	-	+	+	+	-	-	-	-	+/-	-	-	-	+	-	-	+	+	-	+	-	-	+	+	+/-	+/-	-	-	-	-	-	+/-	-	-	-	-	+/-	-	+/-	-	+/-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118838]	11614	-	+	+	+	-	+	-	-	-	-	+	-	+	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7423]	Sample type/isolated from	Daban salt lake
[Ref.: #7423]	Geographic location (country and/or sea, region)	Xinjiang Provence
[Ref.: #7423]	Country	China
[Ref.: #7423]	Country ISO 3 Code	CHN
[Ref.: #7423]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Daban salt lake in Xinjiang region
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #118838]	Sample type/isolated from	Environment, Salt lake
[Ref.: #118838]	Geographic location (country and/or sea, region)	Daban, Xinjiang
[Ref.: #118838]	Country	China
[Ref.: #118838]	Country ISO 3 Code	CHN
[Ref.: #118838]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY351395 (>99% sequence identity) for Halobacillus from Microbeatlas

Aquatic Samples	2677
Soil Samples	2306
Animal Samples	590
Plant Samples	407
Total Samples	5980

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7423]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118838]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7423]	Halobacillus dabanensis strain D-8 16S ribosomal RNA gene, partial sequence	AY351395	1459	GenBank ENA	240302 tax ID
[Ref.: #20218]	Halobacillus dabanensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 569	AB367148	569	GenBank ENA	240302 tax ID	*
[Ref.: #20218]	Halobacillus dabanensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 450	AB367149	450	GenBank ENA	240302 tax ID	*
[Ref.: #20218]	Halobacillus dabanensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 449	AB367150	449	GenBank ENA	240302 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Halobacillus dabanensis CGMCC 1.3704	GCA_900114165	scaffold	GenBank ENA	240302 tax ID
[Ref.: #66792]	Halobacillus dabanensis strain CGMCC 1.3704	240302.16	wgs	PATRIC	240302 tax ID	*
[Ref.: #66792]	Halobacillus dabanensis CGMCC 1.3704	2617270759	draft	JGI IMG/M	240302 tax ID	*

[Ref.: #7423]	GC-content	41.4 mol%
[Ref.: #67770]	GC-content	41.4 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Halobacillus dabanensis CGMCC 1.3704 NCBI: GCA_900114165.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halobacillus dabanensis strain CGMCC 1.3704 PATRIC: 240302.16
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	84.126	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.841	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	93.75	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	72.081	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.853	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.396	no

Availability in culture collections External links

[Ref.: #7423]

Culture collection no.

DSM 18199, CGMCC 1.3704, JCM 12772, CIP 109012

[Ref.: #70998]

SI-ID 265604

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halobacillus andaensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soil.	Wang K, Zhang L, Yang Y, Pan Y, Meng L, Liu H, Hong S, Huang H, Jiang J	Int J Syst Evol Microbiol	10.1099/ijs.0.000198	2015	*
Phylogeny	Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans.	Soto-Ramirez N, Sanchez-Porro C, Rosas-Padilla S, Almodovar K, Jimenez G, Machado-Rodriguez M, Zapata M, Ventosa A, Montalvo-Rodriguez R	Int J Syst Evol Microbiol	10.1099/ijs.0.65008-0	2008	*
Phylogeny	Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China.	Liu WY, Zeng J, Wang L, Dou YT, Yang SS	Int J Syst Evol Microbiol	10.1099/ijs.0.63787-0	2005	*

References

#7423

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18199

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22987

W. Y. Liu,J. Zeng,L. Wang,Y. T. Dou,S. S. Yang: Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. IJSEM 55: 1991 - 1996 2005 ( DOI 10.1099/ijs.0.63787-0 , PubMed 16166700 )

#40089 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70998

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID265604.1 )

#118838 Collection of Institut Pasteur ; Curators of the CIP; CIP 109012
* These data were automatically processed and therefore are not curated

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Halobacillus dabanensis CGMCC 1.3704

Halobacillus dabanensis strain CGMCC 1.3704

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Change proposal