Name: Halobacillus aidingensis AD-6
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1347

Type strain:

Species: Halobacillus aidingensis

Strain Designation: AD-6

Culture col. no.: DSM 18198, CGMCC 1.3703, CIP 109013, JCM 12771

Strain history: CIP <- 2005, JCM <- 2004, W.Y. Liu, China Agric. Univ., Beijing, China: strain AD-6

NCBI tax ID(s): 240303 (species)

SI-ID 265603

CGMCC 1.3703, JCM 12771, DSM 18198, CIP 109013

Special Collections

Section

Halobacillus aidingensis AD-6 is a spore-forming, Gram-positive, motile bacterium that forms circular colonies and has a orange pigmentation.

colony-forming
Gram-positive
motile
pigmented
rod-shaped
spore-forming
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Halobacillus*
	Species	**Halobacillus aidingensis**
	Full Scientific Name (LPSN)	Halobacillus aidingensis Liu et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #22987]	Gram stain	positive
[Ref.: #116167]	Gram stain	positive

[Ref.: #22987]	Cell length	1.0-2.8 µm

[Ref.: #22987]	Cell width	0.6-1.2 µm

[Ref.: #22987]	Cell shape	rod-shaped
[Ref.: #116167]	Cell shape	rod-shaped

[Ref.: #22987]	Motility	yes
[Ref.: #116167]	Motility	yes

[Ref.: #22987]	Flagellum arrangement	peritrichous

[Ref.: #22987]	Colony size	1.0-2.0 mm
[Ref.: #22987]	Colony color	orange
[Ref.: #22987]	Colony shape	circular
[Ref.: #22987]	Incubation period	3 days

[Ref.: #22987]	Pigment production	yes
[Ref.: #22987]	Pigmentation color	orange

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7422]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #7422]

Culture medium growth

[Ref.: #7422]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7422]

Culture medium composition

expand / minimize

[Ref.: #40090]

Culture medium

MEDIUM 665 -for Halobacillus aidigensis

[Ref.: #40090]

Culture medium growth

[Ref.: #40090]

Culture medium composition

expand / minimize

[Ref.: #116167]

Culture medium

CIP Medium 413

[Ref.: #116167]

Culture medium growth

[Ref.: #116167]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7422]	growth	30 °C
[Ref.: #22987]	growth	15.0-40.0 °C
[Ref.: #22987]	optimum	32.0 °C
[Ref.: #40090]	growth	37 °C
[Ref.: #67770]	growth	35 °C

	pH	Kind of pH		pH
[Ref.: #22987]			optimum	7.5
[Ref.: #22987]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #22987]	Spore description	ellipsoidal,central or subterminal position
[Ref.: #22987]	Type of spore	endospore
[Ref.: #22987]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %95.895
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #22987]		NaCl		growth	0.5-20.0	%(w/v)
[Ref.: #22987]		NaCl		optimum	10.0

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #22987]	casein	+	hydrolysis
[Ref.: #22987]	D-fructose ChEBI	+	builds acid from
[Ref.: #22987]	D-galactose ChEBI	-	builds acid from
[Ref.: #22987]	D-glucose ChEBI	+	builds acid from
[Ref.: #22987]	D-mannitol ChEBI	+	builds acid from
[Ref.: #22987]	D-trehalose ChEBI	+	builds acid from
[Ref.: #22987]	D-xylose ChEBI	-	builds acid from
[Ref.: #22987]	esculin ChEBI	-	hydrolysis
[Ref.: #22987]	gelatin ChEBI	+	hydrolysis
[Ref.: #22987]	maltose ChEBI	+	builds acid from
[Ref.: #22987]	nitrate ChEBI	-	reduction
[Ref.: #116167]	nitrate ChEBI	-	reduction
[Ref.: #116167]	nitrite ChEBI	-	reduction
[Ref.: #22987]	starch ChEBI	+	hydrolysis
[Ref.: #22987]	sucrose ChEBI	+	builds acid from
[Ref.: #22987]	tween 80 ChEBI	-	hydrolysis
[Ref.: #22987]	tyrosine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #22987]		acetoin ChEBI	no
[Ref.: #116167]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #22987]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116167]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #22987]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #22987]	catalase	+	1.11.1.6
[Ref.: #116167]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #22987]	cytochrome oxidase	+	1.9.3.1
[Ref.: #22987]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #22987]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116167]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116167]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116167]	oxidase	+
[Ref.: #22987]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #22987]	phosphatase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #22987]	urease	-	3.5.1.5
[Ref.: #116167]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116167]	11615	-	+	+	+	-	+	-	-	-	-	+	-	+	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7422]	Sample type/isolated from	aiding salt lake
[Ref.: #7422]	Geographic location (country and/or sea, region)	Xinjiang region
[Ref.: #7422]	Country	China
[Ref.: #7422]	Country ISO 3 Code	CHN
[Ref.: #7422]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Aiding salt lake in Xinjiang region
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #116167]	Sample type/isolated from	Environment, Salt lake
[Ref.: #116167]	Geographic location (country and/or sea, region)	Aiding, Xinjiang
[Ref.: #116167]	Country	China
[Ref.: #116167]	Country ISO 3 Code	CHN
[Ref.: #116167]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7422]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116167]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7422]	Halobacillus aidingensis strain AD-6 16S ribosomal RNA gene, partial sequence	AY351389	1492	GenBank ENA	240303 tax ID
[Ref.: #67770]	Halobacillus aidingensis strain CGMCC 1.3703 genome assembly, contig: Ga0104470_145, whole genome shotgun sequence	FNIZ01000045	1484	GenBank ENA	240303 tax ID
[Ref.: #20218]	Halobacillus aidingensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: A450	AB367145	450	GenBank ENA	240303 tax ID	*
[Ref.: #20218]	Halobacillus aidingensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: 690	AB367146	690	GenBank ENA	240303 tax ID	*
[Ref.: #20218]	Halobacillus aidingensis genes for 16S ribosomal RNA, internal transcribed spacer 1 and 23S ribosomal RNA, partial and complete sequence, clone: B450	AB367147	450	GenBank ENA	240303 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Halobacillus aidingensis CGMCC 1.3703	GCA_900104185	scaffold	GenBank ENA	240303 tax ID
[Ref.: #66792]	Halobacillus aidingensis strain CGMCC 1.3703	240303.4	wgs	PATRIC	240303 tax ID	*
[Ref.: #66792]	Halobacillus aidingensis CGMCC 1.3703	2675903131	draft	JGI IMG/M	240303 tax ID	*

[Ref.: #7422]	GC-content	42.2 mol%
[Ref.: #67770]	GC-content	42.2 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Halobacillus aidingensis CGMCC 1.3703 NCBI: GCA_900104185.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halobacillus aidingensis strain CGMCC 1.3703 PATRIC: 240303.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	80.221	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.343	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	95.895	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	81.226	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.389	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.475	yes

Availability in culture collections External links

[Ref.: #7422]

Culture collection no.

DSM 18198, CGMCC 1.3703, CIP 109013, JCM 12771

[Ref.: #70997]

SI-ID 265603

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Screening a novel Na+/H+ antiporter gene from a metagenomic library of halophiles colonizing in the Dagong Ancient Brine Well in China.	Xiang W, Zhang J, Li L, Liang H, Luo H, Zhao J, Yang Z, Sun Q	FEMS Microbiol Lett	10.1111/j.1574-6968.2010.01929.x	2010	*
Metabolism	Cloning and characterization of a Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus aidingensis AD-6T.	Zou YJ, Yang LF, Wang L, Yang SS	J Microbiol	10.1007/s12275-008-0009-2	2008	*
Phylogeny	Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China.	Liu WY, Zeng J, Wang L, Dou YT, Yang SS	Int J Syst Evol Microbiol	10.1099/ijs.0.63787-0	2005	*

References

#7422

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18198

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#22987

W. Y. Liu,J. Zeng,L. Wang,Y. T. Dou,S. S. Yang: Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. IJSEM 55: 1991 - 1996 2005 ( DOI 10.1099/ijs.0.63787-0 , PubMed 16166700 )

#40090 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70997

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID265603.1 )

#116167 Collection of Institut Pasteur ; Curators of the CIP; CIP 109013
* These data were automatically processed and therefore are not curated

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Halobacillus aidingensis CGMCC 1.3703

Halobacillus aidingensis strain CGMCC 1.3703

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