Halobacillus profundi (DSM 18394, JCM 14154, JS-Hb4)

Name: Halobacillus profundi (DSM 18394, JCM 14154, JS-Hb4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1350

Type strain

Strain Designation JS-Hb4, IS-Hb4

Culture col. no. DSM 18394 JCM 14154 JS-Hb4 CIP 109643 IS-Hb4

NCBI tax ID(s) 291742

Special Collections DSMZ JCM CIP Literature strains

Links

Halobacillus profundi JS-Hb4 is an obligate aerobe, spore-forming, mesophilic prokaryote that forms circular colonies and was isolated from carbonate rock collected from a 642 m-deep methane cold seep.

spore-forming Gram-positive rod-shaped colony-forming obligate aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 309458

DSM 18394,JCM 14154,CIP 109643

@ref 20215

Last LPSN update 2025-12-16 09:47:05

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Halobacillus

Species Halobacillus profundi

Full scientific name Halobacillus profundi Hua et al. 2007

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
22985	positive	1.0-2.9 µm	0.5-1.0 µm	rod-shaped
121955	positive			rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period
22985	1.5-2.5 mm	pale yellow	circular	3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7523	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
37802	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
121955	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
7523	positive	growth	30	mesophilic
22985	positive	growth	9.0-47.0
22985	positive	optimum	25.0	mesophilic
37802	positive	growth	25	mesophilic
67770	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
22985	positive	optimum	7.5-8.0
22985	positive	growth	5.5-10.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

121955

Oxygen toleranceobligate aerobe

Spore formation

@ref	Spore description	Type of spore	Spore formation
22985	spherical or ellipsoidal,central position	endospore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
22985	NaCl	positive	growth	0.5-30.0 %(w/v)
22985	NaCl		optimum	10.0 %

Murein

@ref	Murein short key	Type
7523	A21.04	A4ß L-Orn-D-Asp

Observation

67770

Observationquinones: MK-7

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
22985	16763 ChEBI	2-oxobutanoate	+	carbon source
22985	30916 ChEBI	2-oxoglutarate	+	carbon source
22985	28644 ChEBI	2-oxopentanoate	+	carbon source
22985	40585 ChEBI	alpha-cyclodextrin	-	hydrolysis
22985	40585 ChEBI	alpha-cyclodextrin	+	carbon source
22985	36219 ChEBI	alpha-lactose	+	carbon source
22985		casein	+	hydrolysis
22985	17057 ChEBI	cellobiose	-	builds acid from
22985	15824 ChEBI	D-fructose	+	builds acid from
22985	12936 ChEBI	D-galactose	-	builds acid from
22985	30612 ChEBI	D-glucarate	+	carbon source
22985	17634 ChEBI	D-glucose	+	builds acid from
22985	16899 ChEBI	D-mannitol	+	builds acid from
22985	16899 ChEBI	D-mannitol	+	carbon source
22985	16523 ChEBI	D-serine	+	carbon source
22985	16443 ChEBI	D-tagatose	-	builds acid from
22985	65327 ChEBI	D-xylose	+	builds acid from
22985	23652 ChEBI	dextrin	-	hydrolysis
22985	17113 ChEBI	erythritol	+	carbon source
22985	4853 ChEBI	esculin	+	hydrolysis
22985	5291 ChEBI	gelatin	-	hydrolysis
22985	28087 ChEBI	glycogen	-	hydrolysis
22985	73804 ChEBI	glycyl L-aspartic acid	+	carbon source
22985	29985 ChEBI	L-glutamate	+	carbon source
22985	17306 ChEBI	maltose	+	builds acid from
22985	29864 ChEBI	mannitol	+	builds acid from
22985	37684 ChEBI	mannose	+	builds acid from
22985	28053 ChEBI	melibiose	-	builds acid from
22985	51850 ChEBI	methyl pyruvate	+	carbon source
22985	17268 ChEBI	myo-inositol	-	builds acid from
22985	506227 ChEBI	N-acetylglucosamine	+	builds acid from
22985	506227 ChEBI	N-acetylglucosamine	+	carbon source
22985	17632 ChEBI	nitrate	+/-	reduction
121955	17632 ChEBI	nitrate	-	reduction
121955	16301 ChEBI	nitrite	-	reduction
22985	16634 ChEBI	raffinose	+	builds acid from
22985	26546 ChEBI	rhamnose	+	builds acid from
22985	41865 ChEBI	sebacic acid	+	carbon source
22985	30911 ChEBI	sorbitol	+	builds acid from
22985	28017 ChEBI	starch	+	hydrolysis
22985	17992 ChEBI	sucrose	+	builds acid from
22985	27082 ChEBI	trehalose	+	builds acid from
22985	53424 ChEBI	tween 20	+	hydrolysis
22985	53423 ChEBI	tween 40	+	carbon source
22985	53423 ChEBI	tween 40	+	hydrolysis
22985	53426 ChEBI	tween 80	+	carbon source
22985	53426 ChEBI	tween 80	+	hydrolysis

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121955	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
22985	catalase	+	1.11.1.6
121955	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
22985	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	-	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
121955	oxidase	+
22985	phenylalanine deaminase	-	4.3.1.5
68382	trypsin	-	3.4.21.4	from API zym
22985	urease	-	3.5.1.5
121955	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121955	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Geologic
#Condition	#Psychrophilic (<10°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture
22985	carbonate rock collected from a 642 m-deep methane cold seep	Kuroshima Knoll	Japan	JPN	Asia	24.13	124.187 24.13/124.187	enrichment medium containing 0.5 % (w/v) Bacto-peptone (Difco),0.25 % yeast extract (Difco),0.1 % D-glucose (Wako) and 15 % NaCl
7523	deep-sea carbonate rock	Kuroshima Knoll	Japan	JPN	Asia
67770	Deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll		Japan	JPN	Asia
121955	Environment, Deep-sea carbonate rock	Kuroshima knoll	Japan	JPN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7523	1	Risk group (German classification) According to TRBA 466
121955	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7523	Halobacillus profundi gene for 16S rRNA, partial sequence	AB189298	1548		291742

GC content

@ref	GC-content (mol%)	Method
7523	43.3
67770	43.3	capillary zone electrophoresis (CZE)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep.	Hua NP, Kanekiyo A, Fujikura K, Yasuda H, Naganuma T	Int J Syst Evol Microbiol	10.1099/ijs.0.64817-0	2007

References

#7523	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18394
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22985	Ngoc-Phuc Hua,Atsuko Kanekiyo,Katsunori Fujikura,Hisato Yasuda,Takeshi Naganuma: Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. IJSEM 57: 1243 - 1249 2007 ( DOI 10.1099/ijs.0.64817-0 , PubMed 17551037 )
#37802	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#121955	Collection of Institut Pasteur ; Curators of the CIP; CIP 109643
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Halobacillus profundi (DSM 18394, JCM 14154, JS-Hb4)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Murein

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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