Saccharopolyspora qijiaojingensis (DSM 45088, KCTC 19235, YIM 91168)

Name: Saccharopolyspora qijiaojingensis (DSM 45088, KCTC 19235, YIM 91168)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13466

Type strain

Culture col. no. DSM 45088 KCTC 19235 YIM 91168 CCTCC AB 206032

NCBI tax ID(s) 453831

Special Collections DSMZ KCTC Wink compendium Literature strains

History <- S.-K. Tang; YIM 91168 <- SK Tang, Yunnan Inst. Microbiol., China

Links

Saccharopolyspora qijiaojingensis DSM 45088 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from salt lake.

spore-forming Gram-positive rod-shaped aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 404340

KCTC 19235,DSM 45088

@ref 20215

Last LPSN update 2026-02-09 11:21:09

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Saccharopolyspora

Species Saccharopolyspora qijiaojingensis

Full scientific name Saccharopolyspora qijiaojingensis Tang et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell shape
29265	positive	rod-shaped
67771	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
19915	Oyster white	10-14 days	ISP 2
19915	Oyster white	10-14 days	ISP 3
19915	Light ivory	10-14 days	ISP 4
19915	Oyster white	10-14 days	ISP 5
19915	Oyster white	10-14 days	ISP 6
19915	Oyster white	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19915		ISP 2
19915		ISP 3
19915		ISP 4
19915		ISP 5
19915		ISP 6
19915		ISP 7

Multimedia

@ref:	12330
multimedia content:	DSM_45088.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45088.jpg
caption:	Medium 1240 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12330	STARCH-MINERAL SALT-AGAR (STMS) + 10%NACL (DSMZ Medium 1240)	Medium recipe at MediaDive	Name: STARCH-MINERAL SALT-AGAR + 10% NACL (DSMZ Medium 1240) Composition: NaCl 100.0 g/l Agar 15.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
19915	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19915	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19915	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19915	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19915	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19915	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
12330	positive	growth	37
19915	positive	optimum	37
29265	positive	growth	20-40
29265	positive	optimum	28
67771	positive	growth	37

Culture pH

@ref	Ability	Type	PH
29265	positive	optimum	7
29265	positive	growth	05-08

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
29265	aerobe
67771	aerobe

Spore formation

29265

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29265	NaCl	positive	growth	06-22 %
29265	NaCl	positive	optimum	13 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29265	28260 ChEBI	galactose	+	carbon source
29265	17716 ChEBI	lactose	+	carbon source
29265	17306 ChEBI	maltose	+	carbon source
29265	29864 ChEBI	mannitol	+	carbon source
29265	37684 ChEBI	mannose	+	carbon source
29265	26546 ChEBI	rhamnose	+	carbon source
29265	27082 ChEBI	trehalose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
29265	gelatinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19915	not determinedn.d.	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Condition	#Saline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12330	salt lake	Xinjiang province	China	CHN	Asia
67771	From salt lake	Xinjiang Province	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence EF687715 (>99% sequence identity) for Saccharopolyspora qijiaojingensis subclade from Microbeatlas

Aquatic Samples	57
Soil Samples	172
Animal Samples	221
Plant Samples	11
Total Samples	461

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12330	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
12330	Saccharopolyspora qijiaojingensis strain YIM 91168 16S ribosomal RNA gene, partial sequence	EF687715	1462		453831

GC content

@ref	GC-content (mol%)	Method
12330	70.1	high performance liquid chromatography (HPLC)
29265	70.1

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mzabimyces algeriensis gen. nov., sp. nov., a halophilic filamentous actinobacterium isolated from a Saharan soil, and proposal of Mzabimycetaceae fam. nov.	Saker R, Bouras N, Zitouni A, Ghoul M, Rohde M, Schumann P, Sproer C, Sabaou N, Klenk HP	Antonie Van Leeuwenhoek	10.1007/s10482-014-0271-8	2014
Phylogeny	Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China.	Guan TW, Wu N, Xia ZF, Ruan JS, Zhang XP, Huang Y, Zhang LL	Extremophiles	10.1007/s00792-011-0369-0	2011
Phylogeny	Saccharopolyspora qijiaojingensis sp. nov., a halophilic actinomycete isolated from a salt lake.	Tang SK, Wang Y, Wu JY, Cao LL, Lou K, Xu LH, Jiang CL, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.009860-0	2009

References

#12330	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45088
#19915	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25683	IJSEM 2166 2009 ( DOI 10.1099/ijs.0.009860-0 , PubMed 19605720 )
#29265	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25683
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Saccharopolyspora qijiaojingensis (DSM 45088, KCTC 19235, YIM 91168)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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