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Strain identifier

BacDive ID: 13466

Type strain: Yes

Species: Saccharopolyspora qijiaojingensis

Strain history: <- SK Tang, Yunnan Inst. Microbiol., China

NCBI tax ID(s): 453831 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 12330

BacDive-ID: 13466

DSM-Number: 45088

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Saccharopolyspora qijiaojingensis DSM 45088 is an aerobe, spore-forming, mesophilic bacterium that was isolated from salt lake.

NCBI tax id

  • NCBI tax id: 453831
  • Matching level: species

strain history

doi: 10.13145/bacdive13466.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Saccharopolyspora
  • species: Saccharopolyspora qijiaojingensis
  • full scientific name: Saccharopolyspora qijiaojingensis Tang et al. 2009

@ref: 12330

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Saccharopolyspora

species: Saccharopolyspora qijiaojingensis

full scientific name: Saccharopolyspora qijiaojingensis Tang et al. 2009

type strain: yes

Morphology

cell morphology

@refgram staincell shape
29265positiverod-shaped
67771positive

colony morphology

@refcolony colorincubation periodmedium used
19915Oyster white10-14 daysISP 2
19915Oyster white10-14 daysISP 3
19915Light ivory10-14 daysISP 4
19915Oyster white10-14 daysISP 5
19915Oyster white10-14 daysISP 6
19915Oyster white10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19915noISP 2
19915noISP 3
19915noISP 4
19915noISP 5
19915noISP 6
19915noISP 7

multimedia

  • @ref: 12330
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45088.jpg
  • caption: Medium 1240 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12330STARCH-MINERAL SALT-AGAR (STMS) + 10%NACL (DSMZ Medium 1240)yeshttps://mediadive.dsmz.de/medium/1240Name: STARCH-MINERAL SALT-AGAR + 10% NACL (DSMZ Medium 1240) Composition: NaCl 100.0 g/l Agar 15.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
19915ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19915ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19915ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19915ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19915ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19915ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
12330positivegrowth37mesophilic
19915positiveoptimum37mesophilic
29265positivegrowth20-40
29265positiveoptimum28mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepH
29265positivegrowth05-08
29265positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29265aerobe
67771aerobe

spore formation

  • @ref: 29265
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29265NaClpositivegrowth06-22 %
29265NaClpositiveoptimum13 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2926528260galactose+carbon source
2926517716lactose+carbon source
2926517306maltose+carbon source
2926529864mannitol+carbon source
2926537684mannose+carbon source
2926526546rhamnose+carbon source
2926527082trehalose+carbon source

enzymes

@refvalueactivityec
29265gelatinase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19915+++-+----++---+-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12330salt lakeXinjiang provinceChinaCHNAsia
67771From salt lakeXinjiang ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_47006.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_21370;97_26668;98_33929;99_47006&stattab=map
  • Last taxonomy: Saccharopolyspora qijiaojingensis subclade
  • 16S sequence: EF687715
  • Sequence Identity:
  • Total samples: 461
  • soil counts: 172
  • aquatic counts: 57
  • animal counts: 221
  • plant counts: 11

Safety information

risk assessment

  • @ref: 12330
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 12330
  • description: Saccharopolyspora qijiaojingensis strain YIM 91168 16S ribosomal RNA gene, partial sequence
  • accession: EF687715
  • length: 1462
  • database: ena
  • NCBI tax ID: 453831

GC content

@refGC-contentmethod
1233070.1high performance liquid chromatography (HPLC)
2926570.1

External links

@ref: 12330

culture collection no.: DSM 45088, KCTC 19235, YIM 91168, CCTCC AB 206032

straininfo link

@refpassport
20218http://www.straininfo.net/strains/844753
20218http://www.straininfo.net/strains/844754
20218http://www.straininfo.net/strains/844752

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19605720Saccharopolyspora qijiaojingensis sp. nov., a halophilic actinomycete isolated from a salt lake.Tang SK, Wang Y, Wu JY, Cao LL, Lou K, Xu LH, Jiang CL, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.009860-02009Arabinose/analysis, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Galactose/analysis, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/chemistry/*classification/genetics/*isolation & purification, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Vitamin K 2/analysis, *Water MicrobiologyGenetics
Phylogeny21461999Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China.Guan TW, Wu N, Xia ZF, Ruan JS, Zhang XP, Huang Y, Zhang LLExtremophiles10.1007/s00792-011-0369-02011China, DNA, Bacterial/isolation & purification, Energy Metabolism, Geologic Sediments/microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Saccharopolyspora/*classification/genetics/isolation & purification/metabolism, Salinity, *Water MicrobiologyEnzymology
Phylogeny25186951Mzabimyces algeriensis gen. nov., sp. nov., a halophilic filamentous actinobacterium isolated from a Saharan soil, and proposal of Mzabimycetaceae fam. nov.Saker R, Bouras N, Zitouni A, Ghoul M, Rohde M, Schumann P, Sproer C, Sabaou N, Klenk HPAntonie Van Leeuwenhoek10.1007/s10482-014-0271-82014Actinobacteria/*classification/cytology/genetics/*isolation & purification, Africa, Northern, Algeria, Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Microscopy, Electron, Scanning, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analysisGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
12330Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45088)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45088
19915Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM45088.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2568310.1099/ijs.0.009860-019605720
29265Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125683
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta