Strain identifier
BacDive ID: 13466
Type strain: 
Species: Saccharopolyspora qijiaojingensis
Strain history: <- SK Tang, Yunnan Inst. Microbiol., China
NCBI tax ID(s): 453831 (species)
version 8.1 (current version)
General
@ref: 12330
BacDive-ID: 13466
DSM-Number: 45088
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped
description: Saccharopolyspora qijiaojingensis DSM 45088 is an aerobe, spore-forming, mesophilic bacterium that was isolated from salt lake.
NCBI tax id
- NCBI tax id: 453831
- Matching level: species
strain history
| @ref | history |
|---|---|
| 12330 | <- S.-K. Tang; YIM 91168 |
| 67771 | <- SK Tang, Yunnan Inst. Microbiol., China |
doi: 10.13145/bacdive13466.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Saccharopolyspora
- species: Saccharopolyspora qijiaojingensis
- full scientific name: Saccharopolyspora qijiaojingensis Tang et al. 2009
@ref: 12330
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Saccharopolyspora
species: Saccharopolyspora qijiaojingensis
full scientific name: Saccharopolyspora qijiaojingensis Tang et al. 2009
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell shape |
|---|---|---|
| 29265 | positive | rod-shaped |
| 67771 | positive |
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19915 | Oyster white | 10-14 days | ISP 2 |
| 19915 | Oyster white | 10-14 days | ISP 3 |
| 19915 | Light ivory | 10-14 days | ISP 4 |
| 19915 | Oyster white | 10-14 days | ISP 5 |
| 19915 | Oyster white | 10-14 days | ISP 6 |
| 19915 | Oyster white | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | medium name |
|---|---|---|
| 19915 | no | ISP 2 |
| 19915 | no | ISP 3 |
| 19915 | no | ISP 4 |
| 19915 | no | ISP 5 |
| 19915 | no | ISP 6 |
| 19915 | no | ISP 7 |
multimedia
- @ref: 12330
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45088.jpg
- caption: Medium 1240 37°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 12330 | STARCH-MINERAL SALT-AGAR (STMS) + 10%NACL (DSMZ Medium 1240) | yes | https://mediadive.dsmz.de/medium/1240 | Name: STARCH-MINERAL SALT-AGAR + 10% NACL (DSMZ Medium 1240) Composition: NaCl 100.0 g/l Agar 15.0 g/l Starch 10.0 g/l (NH4)2SO4 2.0 g/l CaCO3 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water |
| 19915 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19915 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19915 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19915 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19915 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19915 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 12330 | positive | growth | 37 | mesophilic |
| 19915 | positive | optimum | 37 | mesophilic |
| 29265 | positive | growth | 20-40 | |
| 29265 | positive | optimum | 28 | mesophilic |
| 67771 | positive | growth | 37 | mesophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 29265 | positive | growth | 05-08 |
| 29265 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 29265 | aerobe |
| 67771 | aerobe |
spore formation
- @ref: 29265
- spore formation: yes
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 29265 | NaCl | positive | growth | 06-22 % |
| 29265 | NaCl | positive | optimum | 13 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29265 | 28260 | galactose | + | carbon source |
| 29265 | 17716 | lactose | + | carbon source |
| 29265 | 17306 | maltose | + | carbon source |
| 29265 | 29864 | mannitol | + | carbon source |
| 29265 | 37684 | mannose | + | carbon source |
| 29265 | 26546 | rhamnose | + | carbon source |
| 29265 | 27082 | trehalose | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 29265 | gelatinase | + | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | + | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | lipase (C 14) | - | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | - | |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | trypsin | - | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | - | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | - | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | - | 3.2.1.24 |
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 19915 | + | + | + | - | + | - | - | - | - | + | + | - | - | - | + | - | + | - | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 12330 | salt lake | Xinjiang province | China | CHN | Asia |
| 67771 | From salt lake | Xinjiang Province | China | CHN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_47006.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_21370;97_26668;98_33929;99_47006&stattab=map
- Last taxonomy: Saccharopolyspora qijiaojingensis subclade
- 16S sequence: EF687715
- Sequence Identity:
- Total samples: 461
- soil counts: 172
- aquatic counts: 57
- animal counts: 221
- plant counts: 11
Safety information
risk assessment
- @ref: 12330
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 12330
- description: Saccharopolyspora qijiaojingensis strain YIM 91168 16S ribosomal RNA gene, partial sequence
- accession: EF687715
- length: 1462
- database: ena
- NCBI tax ID: 453831
GC content
| @ref | GC-content | method |
|---|---|---|
| 12330 | 70.1 | high performance liquid chromatography (HPLC) |
| 29265 | 70.1 |
External links
@ref: 12330
culture collection no.: DSM 45088, KCTC 19235, YIM 91168, CCTCC AB 206032
straininfo link
- @ref: 82658
- straininfo: 404340
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19605720 | Saccharopolyspora qijiaojingensis sp. nov., a halophilic actinomycete isolated from a salt lake. | Tang SK, Wang Y, Wu JY, Cao LL, Lou K, Xu LH, Jiang CL, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijs.0.009860-0 | 2009 | Arabinose/analysis, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Galactose/analysis, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/chemistry/*classification/genetics/*isolation & purification, Sequence Analysis, DNA, Sodium Chloride/metabolism, Temperature, Vitamin K 2/analysis, *Water Microbiology | Genetics |
| Phylogeny | 21461999 | Saccharopolyspora lacisalsi sp. nov., a novel halophilic actinomycete isolated from a salt lake in Xinjiang, China. | Guan TW, Wu N, Xia ZF, Ruan JS, Zhang XP, Huang Y, Zhang LL | Extremophiles | 10.1007/s00792-011-0369-0 | 2011 | China, DNA, Bacterial/isolation & purification, Energy Metabolism, Geologic Sediments/microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribotyping, Saccharopolyspora/*classification/genetics/isolation & purification/metabolism, Salinity, *Water Microbiology | Enzymology |
| Phylogeny | 25186951 | Mzabimyces algeriensis gen. nov., sp. nov., a halophilic filamentous actinobacterium isolated from a Saharan soil, and proposal of Mzabimycetaceae fam. nov. | Saker R, Bouras N, Zitouni A, Ghoul M, Rohde M, Schumann P, Sproer C, Sabaou N, Klenk HP | Antonie Van Leeuwenhoek | 10.1007/s10482-014-0271-8 | 2014 | Actinobacteria/*classification/cytology/genetics/*isolation & purification, Africa, Northern, Algeria, Bacterial Typing Techniques, Base Composition, Carbohydrates/analysis, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Microscopy, Electron, Scanning, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analysis | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 12330 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45088) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45088 | |||
| 19915 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM45088.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 29265 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 25683 | 28776041 | |
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||||
| 68382 | Automatically annotated from API zym | |||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 82658 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID404340.1 | StrainInfo: A central database for resolving microbial strain identifiers |