Saccharopolyspora flava (DSM 44771, AS 4.1520, IFO 16345)

Name: Saccharopolyspora flava (DSM 44771, AS 4.1520, IFO 16345)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13458

Type strain

Strain Designation 07

Culture col. no. DSM 44771 AS 4.1520 IFO 16345 JCM 10665 KCTC 19905 3 more

NCBI tax ID(s) 95161

Special Collections DSMZ JCM KCTC

History DSMZ <- CGMCC <- Z. Liu; 07 JCM <-- Z. Liu 07. KCTC <- JCM

Links

Saccharopolyspora flava 07 is an aerobe, Gram-positive bacterium that was isolated from garden soil.

Gram-positive aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 100940

AS4.1520,IFO 16345,JCM 10665,KCTC 19905,NBRC 16345,DSM 44771,CGMCC 4.1520

@ref 20215

Last LPSN update 2026-05-29 08:28:03

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Saccharopolyspora

Species Saccharopolyspora flava

Full scientific name Saccharopolyspora flava Lu et al. 2001

Morphology

Cell morphology

@ref	Gram stain	Confidence
67771	positive
125438	positive	90.171
125439	positive	99.127

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
12076	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
12076	positive	growth	28
67770	positive	growth	28
67771	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
67771	aerobe
125438	aerobe	90.621
125439	obligate aerobe	99.578

Observation

67770

Observationquinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	esterase (C 4)	-		from API zym
68369	gelatinase	+		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68369	urease	+	3.5.1.5	from API 20NE
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	enterobactin biosynthesis	100	3 of 3
66794	aminopropanol phosphate biosynthesis	100	2 of 2
66794	kanosamine biosynthesis II	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	ppGpp biosynthesis	100	4 of 4
66794	pentose phosphate pathway	100	11 of 11
66794	ethanol fermentation	100	2 of 2
66794	biotin biosynthesis	100	4 of 4
66794	glycogen metabolism	100	5 of 5
66794	L-lactaldehyde degradation	100	3 of 3
66794	Entner Doudoroff pathway	100	10 of 10
66794	cardiolipin biosynthesis	100	7 of 7
66794	gallate degradation	100	5 of 5
66794	palmitate biosynthesis	100	22 of 22
66794	adipate degradation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	threonine metabolism	100	10 of 10
66794	ribulose monophosphate pathway	100	2 of 2
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	lipoate biosynthesis	100	5 of 5
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	taurine degradation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	valine metabolism	100	9 of 9
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	cyanate degradation	100	3 of 3
66794	butanoate fermentation	100	4 of 4
66794	resorcinol degradation	100	2 of 2
66794	vitamin K metabolism	100	5 of 5
66794	glycine betaine biosynthesis	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	citric acid cycle	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	myo-inositol biosynthesis	90	9 of 10
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	allantoin degradation	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	degradation of sugar acids	88	22 of 25
66794	ketogluconate metabolism	87.5	7 of 8
66794	dTDPLrhamnose biosynthesis	87.5	7 of 8
66794	pyrimidine metabolism	86.67	39 of 45
66794	propanol degradation	85.71	6 of 7
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	phenol degradation	85	17 of 20
66794	purine metabolism	84.04	79 of 94
66794	degradation of aromatic, nitrogen containing compounds	83.33	10 of 12
66794	NAD metabolism	83.33	15 of 18
66794	alanine metabolism	82.76	24 of 29
66794	glycolysis	82.35	14 of 17
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	proline metabolism	81.82	9 of 11
66794	tryptophan metabolism	81.58	31 of 38
66794	methionine metabolism	80.77	21 of 26
66794	factor 420 biosynthesis	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	flavin biosynthesis	80	12 of 15
66794	3-chlorocatechol degradation	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	cellulose degradation	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glutathione metabolism	78.57	11 of 14
66794	degradation of pentoses	78.57	22 of 28
66794	photosynthesis	78.57	11 of 14
66794	heme metabolism	78.57	11 of 14
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	urea cycle	76.92	10 of 13
66794	vitamin B12 metabolism	76.47	26 of 34
66794	non-pathway related	76.32	29 of 38
66794	sulfopterin metabolism	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	cyclohexanol degradation	75	3 of 4
66794	toluene degradation	75	3 of 4
66794	gluconeogenesis	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	3-phenylpropionate degradation	73.33	11 of 15
66794	degradation of hexoses	72.22	13 of 18
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	4-hydroxyphenylacetate degradation	70	7 of 10
66794	oxidative phosphorylation	69.23	63 of 91
66794	degradation of sugar alcohols	68.75	11 of 16
66794	androgen and estrogen metabolism	68.75	11 of 16
66794	lipid metabolism	67.74	21 of 31
66794	acetoin degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	sulfoquinovose degradation	66.67	2 of 3
66794	arginine metabolism	66.67	16 of 24
66794	cysteine metabolism	66.67	12 of 18
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	IAA biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	histidine metabolism	65.52	19 of 29
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	polyamine pathway	65.22	15 of 23
66794	cholesterol biosynthesis	63.64	7 of 11
66794	ascorbate metabolism	63.64	14 of 22
66794	metabolism of disaccharids	63.64	7 of 11
66794	vitamin B6 metabolism	63.64	7 of 11
66794	d-xylose degradation	63.64	7 of 11
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	lysine metabolism	61.9	26 of 42
66794	starch degradation	60	6 of 10
66794	glycine metabolism	60	6 of 10
66794	bile acid biosynthesis, neutral pathway	58.82	10 of 17
66794	tyrosine metabolism	57.14	8 of 14
66794	carotenoid biosynthesis	54.55	12 of 22
66794	sphingosine metabolism	50	3 of 6
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	carnitine metabolism	50	4 of 8
66794	phenylmercury acetate degradation	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	coenzyme M biosynthesis	50	5 of 10
66794	quinate degradation	50	1 of 2
66794	phosphatidylethanolamine bioynthesis	46.15	6 of 13
66794	nitrate assimilation	44.44	4 of 9
66794	lipid A biosynthesis	44.44	4 of 9
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	arachidonate biosynthesis	40	2 of 5
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	hydrogen production	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	sulfate reduction	38.46	5 of 13
66794	arachidonic acid metabolism	33.33	6 of 18
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	mevalonate metabolism	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	vitamin E metabolism	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	methanogenesis from CO2	25	3 of 12
66794	catecholamine biosynthesis	25	1 of 4
66794	daunorubicin biosynthesis	22.22	2 of 9
66794	chlorophyll metabolism	22.22	4 of 18

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
12076	-	+	-	+/-	-	+	+	+/-	+/-	-	+	+	-	-	-	+	+	+	+/-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
12076	-	-	-	-	+	+	+	+	+	+/-	+	+	+	+	+	-	+/-	+	+	+	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12076	garden soil	Beijing, Xishan Mountain	China	CHN	Asia
67770	Garden soil	Xishan Mountain, Beijing	China	CHN	Asia
67771	From grassland soil		China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AF154128 (>99% sequence identity) for Saccharopolyspora flava subclade from Microbeatlas

Aquatic Samples	35
Soil Samples	142
Animal Samples	136
Plant Samples	14
Total Samples	327

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12076	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2599185150 annotated assembly for Saccharopolyspora flava DSM 44771	scaffold	GCA_900116135	95161.3	2599185150	95161	74.4

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Saccharopolyspora flava 16S ribosomal RNA, partial sequence	AF154128	1443		95161

GC content

@ref	GC-content (mol%)	Method
67770	73.1	thermal denaturation, midpoint method (Tm)
67770	70.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: IMG-taxon 2599185150 annotated assembly for Saccharopolyspora flava DSM 44771
NCBI: GCA_900116135

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.58	no
125439	motility	BacteriaNetⓘ	no	88.70	no
125439	gram_stain	BacteriaNetⓘ	positive	99.13	no
125439	spore_formation	BacteriaNetⓘ	yes	60.49	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Saccharopolyspora flava strain DSM 44771
PATRIC: 95161.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.17	yes
125438	anaerobic	anaerobicⓘ	no	94.61	yes
125438	aerobic	aerobicⓘ	yes	90.62	yes
125438	spore-forming	spore-formingⓘ	yes	83.23	no
125438	thermophilic	thermophileⓘ	no	95.19	yes
125438	flagellated	motile2+ⓘ	no	87.59	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	A phylogenetic and evolutionary analysis of antimycin biosynthesis.	Joynt R, Seipke RF.	Microbiology (Reading)	10.1099/mic.0.000572	2018
	Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus.	Baranova AA, Chistov AA, Tyurin AP, Prokhorenko IA, Korshun VA, Biryukov MV, Alferova VA, Zakalyukina YV.	Microorganisms	10.3390/microorganisms8121948	2020
Genetics	New Glycosylated Polyene Macrolides: Refining the Ore from Genome Mining.	Caffrey P, Hogan M, Song Y.	Antibiotics (Basel)	10.3390/antibiotics11030334	2022
	Psi-Footprinting approach for the identification of protein synthesis inhibitor producers.	Handel F, Kulik A, Wex KW, Berscheid A, Saur JS, Winkler A, Wibberg D, Kalinowski J, Brotz-Oesterhelt H, Mast Y	NAR Genom Bioinform	10.1093/nargab/lqac055	2022
Phylogeny	Saccharopolyspora rhizosphaerae sp. nov., an actinomycete isolated from rhizosphere soil in Thailand.	Intra B, Euanorasetr J, Take A, Inahashi Y, Mori M, Panbangred W, Matsumoto A	Int J Syst Evol Microbiol	10.1099/ijsem.0.003307	2019
Phylogeny	Saccharopolyspora tripterygii sp. nov., an endophytic actinomycete isolated from the stem of Tripterygium hypoglaucum.	Li J, Zhao GZ, Qin S, Huang HY, Zhu WY, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.011734-0	2009
Phylogeny	Saccharopolyspora endophytica sp. nov., an endophytic actinomycete isolated from the root of Maytenus austroyunnanensis.	Qin S, Li J, Zhao GZ, Chen HH, Xu LH, Li WJ	Syst Appl Microbiol	10.1016/j.syapm.2008.08.001	2008
Phylogeny	Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp.nov., novel actinomycetes from soil.	Lu Z, Liu Z, Wang L, Zhang Y, Qi W, Goodfellow M	Int J Syst Evol Microbiol	10.1099/00207713-51-2-319	2001

References

#12076	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44771
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Saccharopolyspora flava (DSM 44771, AS 4.1520, IFO 16345)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900116135

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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