Name: Balneicella halophila KHALHBd91
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 133009

Type strain:

Species: Balneicella halophila

Strain Designation: KHALHBd91

Culture col. no.: DSM 28579, JCM 19909

Strain history: M.-L. Fardeau; IRD-MIO, Aix-Marseille Univ., France; KHALBd91.

NCBI tax ID(s): 1537566 (species)

SI-ID 405971

DSM 28579

Special Collections

Section

Balneicella halophila KHALHBd91 is an anaerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from water from a mesothermic spring.

colony-forming
Gram-negative
rod-shaped
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Bacteroidia*
	Order	*Bacteroidales*
	Family	*Balneicellaceae*
	Genus	*Balneicella*
	Species	**Balneicella halophila**
	Full Scientific Name (LPSN)	Balneicella halophila Fadhlaoui et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #24999]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %93.335

[Ref.: #24999]	Cell length	0.5-2.0 µm

[Ref.: #24999]	Cell width	0.5-1.0 µm

[Ref.: #24999]	Cell shape	rod-shaped

[Ref.: #24999]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94

[Ref.: #24999]	Colony size	0.5-1.0 mm
[Ref.: #24999]	Colony color	yellow
[Ref.: #24999]	Colony shape	circular
[Ref.: #24999]	Incubation period	2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24797]

Culture medium

MARINIFILUM MEDIUM (IRD MEDIUM) (DSMZ Medium 1533)

[Ref.: #24797]

Culture medium growth

[Ref.: #24797]

Culture medium link

Medium recipe at MediaDive

[Ref.: #24797]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #24797]	growth	35 °C
[Ref.: #24999]	optimum	37 °C
[Ref.: #24999]	growth	20.0-50.0 °C
[Ref.: #67770]	growth	35 °C

	pH	Kind of pH		pH
[Ref.: #24999]			optimum	7
[Ref.: #24999]			growth	5.5-7.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24999]

Oxygen tolerance

anaerobe

[Ref.: #24999]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #24999]		NaCl		growth	0.5-8.5	%
[Ref.: #24999]		NaCl		optimum	2.5	%

[Ref.: #67770]

Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24999]	casamino acids	-	electron donor
[Ref.: #24999]	elemental sulfur ChEBI	-	electron acceptor
[Ref.: #24999]	fructose ChEBI	-	electron donor
[Ref.: #24999]	fumarate ChEBI	+	electron donor
[Ref.: #24999]	galactose ChEBI	+	electron donor
[Ref.: #24999]	glucose ChEBI	+	electron donor
[Ref.: #24999]	glucose ChEBI	+	fermentation
[Ref.: #24999]	glycerol ChEBI	-	electron donor
[Ref.: #24999]	lactate ChEBI	+	electron donor
[Ref.: #24999]	lactose ChEBI	-	electron donor
[Ref.: #24999]	maltose ChEBI	+	electron donor
[Ref.: #24999]	mannitol ChEBI	-	electron donor
[Ref.: #24999]	mannose ChEBI	-	electron donor
[Ref.: #24999]	melibiose ChEBI	-	electron donor
[Ref.: #24999]	methanol ChEBI	-	electron donor
[Ref.: #24999]	pyruvate ChEBI	+	electron donor
[Ref.: #24999]	sodium nitrate ChEBI	-	electron acceptor
[Ref.: #24999]	sodium nitrite ChEBI	-	electron acceptor
[Ref.: #24999]	sodium sulfate ChEBI	-	electron acceptor
[Ref.: #24999]	sodium sulfite ChEBI	-	electron acceptor
[Ref.: #24999]	sodium thiosulfate ChEBI	-	electron acceptor
[Ref.: #24999]	starch ChEBI	-	electron donor
[Ref.: #24999]	succinate ChEBI	-	electron donor
[Ref.: #24999]	xylose ChEBI	-	electron donor
[Ref.: #24999]	yeast extract	+	electron donor
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24797]	Sample type/isolated from	water from a mesothermic (45°C) spring
[Ref.: #24797]	Geographic location (country and/or sea, region)	Siliana, region of El Krib, Hammam Biadha (35.77524° N 9.26674° E)
[Ref.: #24797]	Country	Tunisia
[Ref.: #24797]	Country ISO 3 Code	TUN
[Ref.: #24797]	Continent	Africa
[Ref.: #24797]	Geographic location	35.7752°/9.2668°

[Ref.: #67770]	Sample type/isolated from	Moderately hot spring of Hammam Biadha
[Ref.: #67770]	Country	Tunisia
[Ref.: #67770]	Country ISO 3 Code	TUN
[Ref.: #67770]	Continent	Africa

[Ref.: #24999]	Enrichment culture	basal culture medium (BM)
[Ref.: #24999]	Enrichment culture composition	containing (g l-1): NH4Cl (1), K2HPO4 (0.3), KH2PO4 (0.3), KCl (0.1), NaCl (20), Yeast extract (1), CaCl2 , 2H2O (0.1), Cysteine-HCl (0.5), Widdel trace element solution (1 ml) (Widdel & Pfennig, 1981) and 1 ml 0.1 % resazurin
[Ref.: #24999]	Enrichment culture temperature	37 ̊C
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Thermal spring

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #24797]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24797]	Bacteroides sp. KhalHBD91 partial 16S rRNA gene, strain KhalHBD91	LM999900	1505	GenBank ENA	1537566 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Balneicella halophila DSM 28579	GCA_003096835	scaffold	GenBank ENA	1537566 tax ID	*
[Ref.: #66792]	Balneicella halophila strain DSM 28579	1537566.3	wgs	PATRIC	1537566 tax ID	*
[Ref.: #66792]	Balneicella halophila DSM 28579	2756170310	draft	JGI IMG/M	1537566 tax ID	*

[Ref.: #24999]	GC-content	35 mol%	high performance liquid chromatography (HPLC)
[Ref.: #24797]	GC-content	35.0 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Balneicella halophila DSM 28579 NCBI: GCA_003096835.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Balneicella halophila DSM 28579 NCBI: GCA_003096835.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	93.335	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	64.584	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	78.84	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.4	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.165	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	94	yes

Availability in culture collections External links

[Ref.: #24797]

Culture collection no.

DSM 28579, JCM 19909

[Ref.: #91283]

SI-ID 405971

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Balneicella halophila gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of Balneicellaceae fam. nov.	Fadhlaoui K, Ben Hania W, Postec A, Hamdi M, Ollivier B, Fardeau ML	Int J Syst Evol Microbiol	10.1099/ijsem.0.001412	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24797

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28579

#24999

K. B. H. Fadhlaoui, Wajdi,Postec, Anne,Hamdi, Moktar,Ollivier, Bernard,Fardeau, Marie-Laure: Balneicella halophila gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of Balneicellaceae fam. nov. IJSEM 66: 4692 - 4696 2016 ( DOI 10.1099/ijsem.0.001412 , PubMed 27604340 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#91283

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405971.1 )

* These data were automatically processed and therefore are not curated

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Balneicella halophila DSM 28579

Balneicella halophila DSM 28579

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