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Strain identifier

BacDive ID: 133009

Type strain: Yes

Species: Balneicella halophila

Strain Designation: KHALHBd91

Strain history: M.-L. Fardeau; IRD-MIO, Aix-Marseille Univ., France; KHALBd91.

NCBI tax ID(s): 1537566 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 24797

BacDive-ID: 133009

DSM-Number: 28579

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Balneicella halophila KHALHBd91 is an anaerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from water from a mesothermic spring.

NCBI tax id

  • NCBI tax id: 1537566
  • Matching level: species

strain history

doi: 10.13145/bacdive133009.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Balneicellaceae
  • genus: Balneicella
  • species: Balneicella halophila
  • full scientific name: Balneicella halophila Fadhlaoui et al. 2016

@ref: 24797

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Balneicellaceae

genus: Balneicella

species: Balneicella halophila

full scientific name: Balneicella halophila Fadhlaoui et al. 2016

strain designation: KHALHBd91

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
24999negative0.5-2.0 µm0.5-1.0 µmrod-shapedno
69480negative99.658

colony morphology

  • @ref: 24999
  • colony size: 0.5-1.0 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 2 days

Culture and growth conditions

culture medium

  • @ref: 24797
  • name: MARINIFILUM MEDIUM (IRD MEDIUM) (DSMZ Medium 1533)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1533
  • composition: Name: MARINIFILUM MEDIUM (IRD MEDIUM) (DSMZ Medium 1533) Composition: NaCl 25.0 g/l Glucose 3.6032 g/l MgCl2 x 6 H2O 3.0 g/l NaHCO3 2.0 g/l Yeast extract 1.0 g/l NH4Cl 1.0 g/l Cysteine HCl 0.5 g/l Na2S x 9 H2O 0.4 g/l K2HPO4 0.3 g/l KH2PO4 0.3 g/l CaCl2 x 2 H2O 0.1 g/l KCl 0.1 g/l HCl 0.001625 g/l FeCl2 x 7 H2O 0.0015 g/l Resazurin 0.001 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l H3BO3 6.2e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l CuCl2 x 2 H2O 1.7e-05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
24999positiveoptimum37mesophilic
24999positivegrowth20.0-50.0
24797positivegrowth35mesophilic
67770positivegrowth35mesophilic

culture pH

@refabilitytypepH
24999positivegrowth5.5-7.8
24999positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
24999anaerobe
69480anaerobe79.161

spore formation

@refspore formationconfidence
24999no
69480no98.968

halophily

@refsaltgrowthtested relationconcentrationhalophily levelconfidence
24999NaClpositivegrowth0.5-8.5 %
24999NaCloptimum2.5 %
69480non-halophilic95.909

observation

  • @ref: 67770
  • observation: quinones: MK-6

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
24999casamino acids-electron donor
24999elemental sulfur-electron acceptor33403
24999fructose-electron donor28757
24999glycerol-electron donor17754
24999lactose-electron donor17716
24999mannitol-electron donor29864
24999mannose-electron donor37684
24999melibiose-electron donor28053
24999methanol-electron donor17790
24999sodium nitrate-electron acceptor63005
24999sodium nitrite-electron acceptor78870
24999sodium sulfate-electron acceptor32149
24999sodium sulfite-electron acceptor86477
24999sodium thiosulfate-electron acceptor132112
24999starch-electron donor28017
24999succinate-electron donor30031
24999xylose-electron donor18222
24999fumarate+electron donor29806
24999galactose+electron donor28260
24999glucose+electron donor17234
24999glucose+fermentation17234
24999lactate+electron donor24996
24999maltose+electron donor17306
24999pyruvate+electron donor15361
24999yeast extract+electron donor

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture temperature
24797water from a mesothermic (45°C) springSiliana, region of El Krib, Hammam Biadha (35.77524° N 9.26674° E)TunisiaTUNAfrica35.77529.2668
24999basal culture medium (BM)containing (g l-1): NH4Cl (1), K2HPO4 (0.3), KH2PO4 (0.3), KCl (0.1), NaCl (20), Yeast extract (1), CaCl2 , 2H2O (0.1), Cysteine-HCl (0.5), Widdel trace element solution (1 ml) (Widdel & Pfennig, 1981) and 1 ml 0.1 % resazurin37
67770Moderately hot spring of Hammam BiadhaTunisiaTUNAfrica

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Thermal spring

Safety information

risk assessment

  • @ref: 24797
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24797
  • description: Bacteroides sp. KhalHBD91 partial 16S rRNA gene, strain KhalHBD91
  • accession: LM999900
  • length: 1505
  • database: ena
  • NCBI tax ID: 1537566

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Balneicella halophila DSM 28579GCA_003096835scaffoldncbi1537566
66792Balneicella halophila strain DSM 285791537566.3wgspatric1537566
66792Balneicella halophila DSM 285792756170310draftimg1537566
66792Klebsiella pneumoniae Bio45GCA_022699345completepatric573

GC content

@refGC-contentmethod
2499935high performance liquid chromatography (HPLC)
2479735.0high performance liquid chromatography (HPLC)

External links

@ref: 24797

culture collection no.: DSM 28579, JCM 19909

literature

  • topic: Phylogeny
  • Pubmed-ID: 27604340
  • title: Balneicella halophila gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of Balneicellaceae fam. nov.
  • authors: Fadhlaoui K, Ben Hania W, Postec A, Hamdi M, Ollivier B, Fardeau ML
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001412
  • year: 2016
  • mesh: Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hot Springs/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tunisia, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24797Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28579Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28579)
24999K. B. H. Fadhlaoui, Wajdi,Postec, Anne,Hamdi, Moktar,Ollivier, Bernard,Fardeau, Marie-LaureBalneicella halophila gen. nov., sp. nov., an anaerobic bacterium, isolated from a thermal spring and description of Balneicellaceae fam. nov10.1099/ijsem.0.001412IJSEM 66: 4692-4696 201627604340
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes