Name: Actinophytocola algeriensis MB20
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 132566

Type strain:

Species: Actinophytocola algeriensis

Strain Designation: MB20

Culture col. no.: DSM 46746, CECT 8960

Strain history: <- N. Sabaou, Alger, Algeria; MB20

NCBI tax ID(s): 1768010 (species)

SI-ID 404241

DSM 46746

Special Collections

Section

Actinophytocola algeriensis MB20 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from soil from a palm grove.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinophytocola*
	Species	**Actinophytocola algeriensis**
	Full Scientific Name (LPSN)	Actinophytocola algeriensis Bouznada et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43602]	Gram stain	positive

[Ref.: #43602]	Motility	no

[Ref.: #43602]	Colony color	White
[Ref.: #43602]	Cultivation medium used	ISP 2

Multimedia content

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Caption:

Medium 535 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #24355]		growth	28 °C
[Ref.: #43602]		growth	20-40 °C
[Ref.: #43602]	no	growth	15 °C
[Ref.: #43602]	no	growth	45 °C

	pH	Kind of pH		pH
[Ref.: #43602]			growth	6-9

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #69481]

Ability of spore formation

yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43602]		NaCl		growth	0-4	%(w/v)
[Ref.: #43602]		NaCl		optimum	0-2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43602]	casein	+	hydrolysis
[Ref.: #43602]	cellobiose ChEBI	+	carbon source
[Ref.: #43602]	D-fructose ChEBI	+	carbon source
[Ref.: #43602]	D-galactose ChEBI	+	carbon source
[Ref.: #43602]	D-glucose ChEBI	-	carbon source
[Ref.: #43602]	D-mannitol ChEBI	+	carbon source
[Ref.: #43602]	D-ribose ChEBI	+	carbon source
[Ref.: #43602]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43602]	D-xylose ChEBI	+	carbon source
[Ref.: #43602]	gelatin ChEBI	+	hydrolysis
[Ref.: #43602]	guanine ChEBI	-	hydrolysis
[Ref.: #43602]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #43602]	L-arabinose ChEBI	-	carbon source
[Ref.: #43602]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43602]	lactose ChEBI	-	carbon source
[Ref.: #43602]	maltose ChEBI	+	carbon source
[Ref.: #43602]	melibiose ChEBI	+	carbon source
[Ref.: #43602]	methyl D-glucoside ChEBI	-	carbon source
[Ref.: #43602]	myo-inositol ChEBI	-	carbon source
[Ref.: #43602]	raffinose ChEBI	-	carbon source
[Ref.: #43602]	sucrose ChEBI	+	carbon source
[Ref.: #43602]	trehalose ChEBI	+	carbon source
[Ref.: #43602]	tyrosine ChEBI	-	hydrolysis
[Ref.: #43602]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43602]		catalase	+	1.11.1.6
[Ref.: #43602]		nitrate reductase	-	1.7.99.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24355]	Sample type/isolated from	soil from a palm grove
[Ref.: #24355]	Geographic location (country and/or sea, region)	Ghardaia, Berriane (32° 29' N 3° 41' E)
[Ref.: #24355]	Country	Algeria
[Ref.: #24355]	Country ISO 3 Code	DZA
[Ref.: #24355]	Continent	Africa
[Ref.: #24355]	Geographic location	32.4833°/3.6833°

[Ref.: #43602]	Sample type/isolated from	Saharan soil from Berriane palm grove
[Ref.: #43602]	Geographic location (country and/or sea, region)	Ghardaia, Mzab region
[Ref.: #43602]	Country	Algeria
[Ref.: #43602]	Country ISO 3 Code	DZA
[Ref.: #43602]	Continent	Africa
[Ref.: #43602]	Geographic location	32.4830°/3.6830°
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Grove (Orchard)
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Tree

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KU242749 (>99% sequence identity) for Actinophytocola algeriensis from Microbeatlas

Aquatic Samples	6
Soil Samples	70
Animal Samples	5
Plant Samples	4
Total Samples	85

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24355]	Actinophytocola algeriensis strain MB20 16S ribosomal RNA gene, partial sequence	KU242749	1440	GenBank ENA	1768010 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinophytocola algeriensis DSM 46746	GCA_014874055	contig	GenBank ENA	1768010 tax ID	*
[Ref.: #66792]	Actinophytocola algeriensis CECT 8960	GCA_014203735	scaffold	GenBank ENA	1768010 tax ID	*
[Ref.: #66792]	Actinophytocola algeriensis strain CECT 8960	1768010.3	wgs	PATRIC	1768010 tax ID	*
[Ref.: #66792]	Actinophytocola algeriensis strain DSM 46746	1768010.4	wgs	PATRIC	1768010 tax ID	*
[Ref.: #66792]	Actinophytocola algeriensis CECT 8960	2863340477	draft	JGI IMG/M	1768010 tax ID	*
[Ref.: #66792]	Actinophytocola algeriensis DSM 46746	2870820038	draft	JGI IMG/M	1768010 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinophytocola algeriensis strain DSM 46746 PATRIC: 1768010.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinophytocola algeriensis CECT 8960 NCBI: GCA_014203735.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.411	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.169	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	88.135	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	86.559	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.994	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	88	no

Availability in culture collections External links

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Culture collection no.

DSM 46746, CECT 8960

[Ref.: #90883]

SI-ID 404241

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinophytocola algeriensis sp. nov., an actinobacterium isolated from Saharan soil.	Bouznada K, Bouras N, Schumann P, Sproer C, Sabaou N, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijsem.0.001136	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24355

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46746

#43602

Khaoula Bouznada, Noureddine Bouras, Peter Schumann, Cathrin Spröer, Nasserdine Sabaou, Hans-Peter Klenk: Actinophytocola algeriensis sp. nov., an actinobacterium isolated from Saharan soil. IJSEM 66: 2760 - 2765 2016 ( DOI 10.1099/ijsem.0.001136 , PubMed 27151064 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90883

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404241.1 )

* These data were automatically processed and therefore are not curated

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Actinophytocola algeriensis strain DSM 46746

Actinophytocola algeriensis CECT 8960

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