Strain identifier
BacDive ID: 132566
Type strain: 
Species: Actinophytocola algeriensis
Strain Designation: MB20
Strain history: <- N. Sabaou, Alger, Algeria; MB20
NCBI tax ID(s): 1768010 (species)
version 8.1 (current version)
General
@ref: 24355
BacDive-ID: 132566
DSM-Number: 46746
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive
description: Actinophytocola algeriensis MB20 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from soil from a palm grove.
NCBI tax id
- NCBI tax id: 1768010
- Matching level: species
strain history
- @ref: 24355
- history: <- N. Sabaou, Alger, Algeria; MB20
doi: 10.13145/bacdive132566.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Actinophytocola
- species: Actinophytocola algeriensis
- full scientific name: Actinophytocola algeriensis Bouznada et al. 2016
@ref: 24355
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Actinophytocola
species: Actinophytocola algeriensis
full scientific name: Actinophytocola algeriensis Bouznada et al. 2016
strain designation: MB20
type strain: yes
Morphology
cell morphology
| @ref | gram stain | motility | confidence |
|---|---|---|---|
| 43602 | positive | no | |
| 69480 | positive | 100 |
colony morphology
- @ref: 43602
- colony color: White
- medium used: ISP 2
multimedia
- @ref: 24355
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46746.jpg
- caption: Medium 535 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 43602 | ISP 2 | yes | ||
| 24355 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://mediadive.dsmz.de/medium/535 | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 43602 | positive | growth | 20-40 | |
| 43602 | no | growth | 15 | psychrophilic |
| 43602 | no | growth | 45 | thermophilic |
| 24355 | positive | growth | 28 | mesophilic |
culture pH
- @ref: 43602
- ability: positive
- type: growth
- pH: 6-9
- PH range: alkaliphile
Physiology and metabolism
oxygen tolerance
- @ref: 43602
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 43602 | NaCl | positive | growth | 0-4 %(w/v) |
| 43602 | NaCl | positive | optimum | 0-2 %(w/v) |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 43602 | 17634 | D-glucose | - | carbon source |
| 43602 | 30849 | L-arabinose | - | carbon source |
| 43602 | 17716 | lactose | - | carbon source |
| 43602 | 37657 | methyl D-glucoside | - | carbon source |
| 43602 | 17268 | myo-inositol | - | carbon source |
| 43602 | 16634 | raffinose | - | carbon source |
| 43602 | 16235 | guanine | - | hydrolysis |
| 43602 | 17368 | hypoxanthine | - | hydrolysis |
| 43602 | 18186 | tyrosine | - | hydrolysis |
| 43602 | 15318 | xanthine | - | hydrolysis |
| 43602 | 17057 | cellobiose | + | carbon source |
| 43602 | 15824 | D-fructose | + | carbon source |
| 43602 | 12936 | D-galactose | + | carbon source |
| 43602 | 16899 | D-mannitol | + | carbon source |
| 43602 | 16988 | D-ribose | + | carbon source |
| 43602 | 17924 | D-sorbitol | + | carbon source |
| 43602 | 65327 | D-xylose | + | carbon source |
| 43602 | 62345 | L-rhamnose | + | carbon source |
| 43602 | 17306 | maltose | + | carbon source |
| 43602 | 28053 | melibiose | + | carbon source |
| 43602 | 17992 | sucrose | + | carbon source |
| 43602 | 27082 | trehalose | + | carbon source |
| 43602 | casein | + | hydrolysis | |
| 43602 | 5291 | gelatin | + | hydrolysis |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 43602 | nitrate reductase | - | 1.7.99.4 |
| 43602 | catalase | + | 1.11.1.6 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 24355 | soil from a palm grove | Ghardaia, Berriane (32° 29' N 3° 41' E) | Algeria | DZA | Africa | 32.4833 | 3.6833 |
| 43602 | Saharan soil from Berriane palm grove | Ghardaia, Mzab region | Algeria | DZA | Africa | 32.483 | 3.683 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Agriculture | #Grove (Orchard) |
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants | #Tree |
taxonmaps
- @ref: 69479
- File name: preview.99_48928.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_13737;97_16724;98_30670;99_48928&stattab=map
- Last taxonomy: Actinophytocola algeriensis
- 16S sequence: KU242749
- Sequence Identity:
- Total samples: 85
- soil counts: 70
- aquatic counts: 6
- animal counts: 5
- plant counts: 4
Safety information
risk assessment
- @ref: 24355
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24355
- description: Actinophytocola algeriensis strain MB20 16S ribosomal RNA gene, partial sequence
- accession: KU242749
- length: 1440
- database: ena
- NCBI tax ID: 1768010
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Actinophytocola algeriensis DSM 46746 | GCA_014874055 | contig | ncbi | 1768010 |
| 66792 | Actinophytocola algeriensis CECT 8960 | GCA_014203735 | scaffold | ncbi | 1768010 |
| 66792 | Actinophytocola algeriensis strain CECT 8960 | 1768010.3 | wgs | patric | 1768010 |
| 66792 | Actinophytocola algeriensis strain DSM 46746 | 1768010.4 | wgs | patric | 1768010 |
| 66792 | Actinophytocola algeriensis CECT 8960 | 2863340477 | draft | img | 1768010 |
| 66792 | Actinophytocola algeriensis DSM 46746 | 2870820038 | draft | img | 1768010 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| flagellated | no | 97.177 | no |
| gram-positive | yes | 88.326 | yes |
| anaerobic | no | 99.273 | yes |
| aerobic | yes | 92.357 | yes |
| halophile | no | 88.978 | no |
| spore-forming | yes | 93.376 | no |
| glucose-util | yes | 83.702 | yes |
| thermophile | no | 99.19 | no |
| motile | no | 92.29 | yes |
| glucose-ferment | no | 91.305 | no |
External links
@ref: 24355
culture collection no.: DSM 46746, CECT 8960
straininfo link
- @ref: 90883
- straininfo: 404241
literature
- topic: Phylogeny
- Pubmed-ID: 27151064
- title: Actinophytocola algeriensis sp. nov., an actinobacterium isolated from Saharan soil.
- authors: Bouznada K, Bouras N, Schumann P, Sproer C, Sabaou N, Klenk HP
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001136
- year: 2016
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Africa, Northern, Algeria, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 24355 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-46746 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46746) | |||
| 43602 | Khaoula Bouznada, Noureddine Bouras, Peter Schumann, Cathrin Spröer, Nasserdine Sabaou, Hans-Peter Klenk | Actinophytocola algeriensis sp. nov., an actinobacterium isolated from Saharan soil | 10.1099/ijsem.0.001136 | IJSEM 66: 2760-2765 2016 | 27151064 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
| 90883 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID404241.1 |