Name: Fictibacillus halophilus DSM 100124
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132548

Type strain:

Species: Fictibacillus halophilus

Culture col. no.: DSM 100124, KCTC 33758, MCC 2765, AS 8

Strain history: <- R. Lal, Univ. Delhi, Dept. Botany, India; AS8 <- A. Sharma

NCBI tax ID(s): 1610490 (species)

Section

Fictibacillus halophilus DSM 100124 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from microbial mat of a hot spring deposited around and above the hot water discharge.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Fictibacillus*
	Species	**Fictibacillus halophilus**
	Full Scientific Name (LPSN)	Fictibacillus halophilus Sharma et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43636]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43636]	Cell length	1.4-1.5 µm

[Ref.: #43636]	Cell width	0.6-0.7 µm

[Ref.: #43636]	Cell shape	rod-shaped

[Ref.: #43636]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.051

[Ref.: #43636]	Colony color	Pale peach
[Ref.: #43636]	Colony shape	circular
[Ref.: #43636]	Incubation period	1 day
[Ref.: #43636]	Cultivation medium used	Luria Bertani agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43636]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #43636]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43636]			growth	28-45 °C
[Ref.: #43636]			optimum	37 °C

[Ref.: #43636]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43636]			optimum	7
[Ref.: #43636]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43636]

Oxygen tolerance

aerobe

[Ref.: #43636]	Spore description	oval; in subterminal, swollen sporangia
[Ref.: #43636]	Type of spore	spore
[Ref.: #43636]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43636]		NaCl		growth	0-12	%(w/v)
[Ref.: #43636]		NaCl		optimum	5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43636]	adipate ChEBI	-	assimilation
[Ref.: #43636]	arbutin ChEBI	+	assimilation
[Ref.: #43636]	arbutin ChEBI	+	builds acid from
[Ref.: #43636]	casein	+	hydrolysis
[Ref.: #43636]	cellobiose ChEBI	+	assimilation
[Ref.: #43636]	cellobiose ChEBI	+	carbon source
[Ref.: #43636]	citrate ChEBI	-	assimilation
[Ref.: #43636]	D-fructose ChEBI	+	assimilation
[Ref.: #43636]	D-galactose ChEBI	-	assimilation
[Ref.: #43636]	D-glucose ChEBI	+	assimilation
[Ref.: #43636]	D-glucose ChEBI	+	builds acid from
[Ref.: #43636]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43636]	D-mannose ChEBI	-	assimilation
[Ref.: #43636]	D-mannose ChEBI	-	builds acid from
[Ref.: #43636]	D-ribose ChEBI	+	assimilation
[Ref.: #43636]	D-ribose ChEBI	+	builds acid from
[Ref.: #43636]	D-ribose ChEBI	+	carbon source
[Ref.: #43636]	D-sorbitol ChEBI	-	assimilation
[Ref.: #43636]	D-xylose ChEBI	-	builds acid from
[Ref.: #43636]	esculin ChEBI	+	hydrolysis
[Ref.: #43636]	fructose ChEBI	+	builds acid from
[Ref.: #43636]	fructose ChEBI	+	carbon source
[Ref.: #43636]	galactitol ChEBI	-	builds acid from
[Ref.: #43636]	gelatin ChEBI	+	hydrolysis
[Ref.: #43636]	glucose ChEBI	+	carbon source
[Ref.: #43636]	hypoxanthine ChEBI	+	hydrolysis
[Ref.: #43636]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43636]	lactose ChEBI	-	builds acid from
[Ref.: #43636]	maltose ChEBI	+	assimilation
[Ref.: #43636]	maltose ChEBI	+	builds acid from
[Ref.: #43636]	maltose ChEBI	+	carbon source
[Ref.: #43636]	melibiose ChEBI	-	builds acid from
[Ref.: #43636]	myo-inositol ChEBI	-	assimilation
[Ref.: #43636]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43636]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43636]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #43636]	nitrate ChEBI	-	reduction
[Ref.: #43636]	nitrite ChEBI	-	reduction
[Ref.: #43636]	raffinose ChEBI	-	builds acid from
[Ref.: #43636]	rhamnose ChEBI	-	assimilation
[Ref.: #43636]	salicin ChEBI	+	assimilation
[Ref.: #43636]	salicin ChEBI	+	builds acid from
[Ref.: #43636]	salicin ChEBI	+	carbon source
[Ref.: #43636]	starch ChEBI	+	hydrolysis
[Ref.: #43636]	sucrose ChEBI	+	assimilation
[Ref.: #43636]	sucrose ChEBI	+	builds acid from
[Ref.: #43636]	sucrose ChEBI	+	carbon source
[Ref.: #43636]	trehalose ChEBI	+	assimilation
[Ref.: #43636]	trehalose ChEBI	+	builds acid from
[Ref.: #43636]	trehalose ChEBI	+	carbon source
[Ref.: #43636]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43636]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43636]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43636]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43636]	urea ChEBI	-	hydrolysis
[Ref.: #43636]	xanthine ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43636]	amikacin ChEBI	yes	30	µg (disc)
[Ref.: #43636]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #43636]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43636]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #43636]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43636]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #43636]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #43636]	oxytetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43636]	penicillin ChEBI				yes	10	Unit (disc)
[Ref.: #43636]	polymyxin b ChEBI	yes	300	µg (disc)
[Ref.: #43636]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #43636]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #43636]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43636]		hydrogen sulfide ChEBI	no
[Ref.: #43636]		indole ChEBI	no

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43636]		catalase	+	1.11.1.6
[Ref.: #43636]		cytochrome oxidase	-	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43636]

Fatty acid	Percentage
C15:0 iso	23.6
C15:0 anteiso	20.5
C16:1ω5c	18.9
C16:1ω7c / C16:1ω6c	13.9
iso-C17:1 / anteiso-C17:1	3.7
C16:0 3OH	3.4
C16:0 2OH	2.1
C14:0 3OH / C16:1 iso I	1.9
C16:0	1.8
C14:0	1.6
C14:0 iso	1.2
C15:0 iso 3OH	1.1
C18:1ω7c / C18:1ω6c	1
C17:0 iso	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24337]	Sample type/isolated from	microbial mat of a hot spring deposited around and above the hot water discharge
[Ref.: #24337]	Geographic location (country and/or sea, region)	Himachal Pradesh, Kullu District, Manikaran (32° 01' 34.8'' N 77° 20' 50.3'' E)
[Ref.: #24337]	Country	India
[Ref.: #24337]	Country ISO 3 Code	IND
[Ref.: #24337]	Continent	Asia
[Ref.: #24337]	Geographic location	32.0263°/77.3473°

[Ref.: #43636]	Sample type/isolated from	Microbial mats of hot springs at Manikaran
[Ref.: #43636]	Geographic location (country and/or sea, region)	Manikaran, Himachal Pradesh
[Ref.: #43636]	Country	India
[Ref.: #43636]	Country ISO 3 Code	IND
[Ref.: #43636]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Thermophilic (>45°C)	-
#Environmental	#Aquatic	#Thermal spring
#Environmental	#Microbial community	#Microbial mat

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KP265300 (>99% sequence identity) for Fictibacillus from Microbeatlas

Aquatic Samples	5486
Soil Samples	12226
Animal Samples	4217
Plant Samples	1938
Total Samples	23867

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #24337]	Fictibacillus halophilus strain AS8 16S ribosomal RNA gene, partial sequence	KP265300	1446	ENA	1610490 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Fictibacillus halophilus KCTC 33758	GCA_016401385	contig	GenBank	1610490 tax ID	*
[Ref.: #66792]	Fictibacillus halophilus strain KCTC 33758	1610490.3	wgs	PATRIC	1610490 tax ID	*
[Ref.: #66792]	Fictibacillus halophilus DSM 100124	2928578658	draft	JGI IMG/M	1610490 tax ID	*

[Ref.: #43636]	GC-content	46.9 mol%	real time PCR (RTD-PCR)
[Ref.: #24337]	GC-content	46.9 mol%	fluorimetric

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Fictibacillus halophilus strain KCTC 33758 PATRIC: 1610490.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Fictibacillus halophilus strain KCTC 33758 PATRIC: 1610490.3
[Ref.: #69480]	motile	yes	90.253	yes
[Ref.: #69480]	gram-positive	yes	91.168	yes
[Ref.: #69480]	anaerobic	no	98.108	yes
[Ref.: #69480]	aerobic	yes	91.064	yes
[Ref.: #69480]	halophile	no	52.909	no
[Ref.: #69480]	spore-forming	yes	96.272	yes
[Ref.: #69480]	thermophile	no	96.866	yes
[Ref.: #69480]	glucose-util	yes	88.985	yes
[Ref.: #69480]	flagellated	yes	77.463	no
[Ref.: #69480]	glucose-ferment	no	93.986	no

Availability in culture collections External links

[Ref.: #24337]

Culture collection no.

DSM 100124, KCTC 33758, MCC 2765, AS 8

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Fictibacillus iocasae sp. nov., isolated from the deep-sea sediment in Pacmanus, Manus Basin.	Wang HL, Zhang J, Sun L	Arch Microbiol	10.1007/s00203-018-1527-x	2018	*
Phylogeny	Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range.	Sharma A, Kohli P, Singh Y, Schumann P, Lal R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001051	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#24337

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100124

#43636

Anukriti Sharma, Puneet Kohli, Yogendra Singh, Peter Schumann, Rup Lal: Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range. IJSEM 66: 2409 - 2416 2016 ( DOI 10.1099/ijsem.0.001051 , PubMed 27031366 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Fictibacillus halophilus strain KCTC 33758

Fictibacillus halophilus strain KCTC 33758

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