Strain identifier

BacDive ID: 132548

Type strain: Yes

Species: Fictibacillus halophilus

Strain history: <- R. Lal, Univ. Delhi, Dept. Botany, India; AS8 <- A. Sharma

NCBI tax ID(s): 1610490 (species)

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General

@ref: 24337

BacDive-ID: 132548

DSM-Number: 100124

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Fictibacillus halophilus DSM 100124 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from microbial mat of a hot spring deposited around and above the hot water discharge.

NCBI tax id

  • NCBI tax id: 1610490
  • Matching level: species

strain history

  • @ref: 24337
  • history: <- R. Lal, Univ. Delhi, Dept. Botany, India; AS8 <- A. Sharma

doi: 10.13145/bacdive132548.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Fictibacillus
  • species: Fictibacillus halophilus
  • full scientific name: Fictibacillus halophilus Sharma et al. 2016

@ref: 24337

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Fictibacillus

species: Fictibacillus halophilus

full scientific name: Fictibacillus halophilus Sharma et al. 2016

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43636positive1.4-1.5 µm0.6-0.7 µmrod-shapedyes
69480yes97.051
69480positive100

colony morphology

  • @ref: 43636
  • colony color: Pale peach
  • colony shape: circular
  • incubation period: 1 day
  • medium used: Luria Bertani agar

Culture and growth conditions

culture medium

  • @ref: 43636
  • name: LB (Luria-Bertani) MEDIUM
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43636positivegrowth28-45
43636positiveoptimum37mesophilic

culture pH

@refabilitytypepH
43636positivegrowth6-8
43636positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43636
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
43636oval; in subterminal, swollen sporangiasporeyes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
43636NaClpositivegrowth0-12 %(w/v)
43636NaClpositiveoptimum5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4363617128adipate-assimilation
4363616947citrate-assimilation
4363612936D-galactose-assimilation
4363616024D-mannose-assimilation
4363617924D-sorbitol-assimilation
4363617268myo-inositol-assimilation
4363626546rhamnose-assimilation
4363616899D-mannitol-builds acid from
4363616024D-mannose-builds acid from
4363665327D-xylose-builds acid from
4363616813galactitol-builds acid from
4363617716lactose-builds acid from
4363628053melibiose-builds acid from
4363617268myo-inositol-builds acid from
4363616634raffinose-builds acid from
4363653424tween 20-hydrolysis
4363653423tween 40-hydrolysis
4363653425tween 60-hydrolysis
4363653426tween 80-hydrolysis
4363616199urea-hydrolysis
4363617632nitrate-reduction
4363616301nitrite-reduction
4363618305arbutin+assimilation
4363617057cellobiose+assimilation
4363615824D-fructose+assimilation
4363617634D-glucose+assimilation
4363616988D-ribose+assimilation
4363617306maltose+assimilation
43636506227N-acetylglucosamine+assimilation
4363617814salicin+assimilation
4363617992sucrose+assimilation
4363627082trehalose+assimilation
4363618305arbutin+builds acid from
4363617634D-glucose+builds acid from
4363616988D-ribose+builds acid from
4363628757fructose+builds acid from
4363630849L-arabinose+builds acid from
4363617306maltose+builds acid from
43636506227N-acetylglucosamine+builds acid from
4363617814salicin+builds acid from
4363617992sucrose+builds acid from
4363627082trehalose+builds acid from
4363617057cellobiose+carbon source
4363616988D-ribose+carbon source
4363628757fructose+carbon source
4363617234glucose+carbon source
4363617306maltose+carbon source
4363617814salicin+carbon source
4363617992sucrose+carbon source
4363627082trehalose+carbon source
43636casein+hydrolysis
436364853esculin+hydrolysis
436365291gelatin+hydrolysis
4363617368hypoxanthine+hydrolysis
4363628017starch+hydrolysis
4363615318xanthine+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4363628077rifampicinyesyes5 µg (disc)
4363617833gentamicinyesyes10 µg (disc)
4363627701oxytetracyclineyesyes30 µg (disc)
436362637amikacinyesyes30 µg (disc)
4363617698chloramphenicolyesyes30 µg (disc)
4363628001vancomycinyesyes30 µg (disc)
43636100241ciprofloxacinyesyes5 µg (disc)
436368309polymyxin byesyes300 µg (disc)
43636100147nalidixic acidyesyes30 µg (disc)
436366104kanamycinyesyes30 µg (disc)
4363627902tetracyclineyesyes30 µg (disc)
4363628971ampicillinyesyes10 µg (disc)
4363617334penicillinyesyes10 Unit (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4363616136hydrogen sulfideno
4363635581indoleno

enzymes

@refvalueactivityec
43636catalase+1.11.1.6
43636cytochrome oxidase-1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43636C15:0 anteiso20.5
    43636C14:01.6
    43636C14:0 3OH / C16:1 iso I1.9
    43636C16:01.8
    43636C16:0 2OH2.1
    43636C16:0 3OH3.4
    43636C16:1ω5c18.9
    43636C16:1ω7c / C16:1ω6c13.9
    43636C18:1ω7c / C18:1ω6c1
    43636C14:0 iso1.2
    43636C15:0 iso23.6
    43636C15:0 iso 3OH1.1
    43636C17:0 iso1
    43636iso-C17:1 / anteiso-C17:13.7
  • type of FA analysis: whole cell analysis
  • incubation medium: LB agar plates
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 1
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.0B
  • library/peak naming table: TSBA60
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
24337microbial mat of a hot spring deposited around and above the hot water dischargeHimachal Pradesh, Kullu District, Manikaran (32° 01' 34.8'' N 77° 20' 50.3'' E)IndiaINDAsia32.026377.3473
43636Microbial mats of hot springs at ManikaranManikaran, Himachal PradeshIndiaINDAsia

isolation source categories

Cat1Cat2Cat3
#Condition#Thermophilic (>45°C)
#Environmental#Aquatic#Thermal spring
#Environmental#Microbial community#Microbial mat

taxonmaps

  • @ref: 69479
  • File name: preview.99_3603.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1870;97_2247;98_2758;99_3603&stattab=map
  • Last taxonomy: Fictibacillus
  • 16S sequence: KP265300
  • Sequence Identity:
  • Total samples: 23867
  • soil counts: 12226
  • aquatic counts: 5486
  • animal counts: 4217
  • plant counts: 1938

Sequence information

16S sequences

  • @ref: 24337
  • description: Fictibacillus halophilus strain AS8 16S ribosomal RNA gene, partial sequence
  • accession: KP265300
  • length: 1446
  • database: ena
  • NCBI tax ID: 1610490

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Fictibacillus halophilus KCTC 33758GCA_016401385contigncbi1610490
66792Fictibacillus halophilus strain KCTC 337581610490.3wgspatric1610490
66792Fictibacillus halophilus DSM 1001242928578658draftimg1610490

GC content

@refGC-contentmethod
4363646.9real time PCR (RTD-PCR)
2433746.9fluorimetric

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes90.253yes
gram-positiveyes91.168yes
anaerobicno98.108yes
aerobicyes91.064yes
halophileno52.909no
spore-formingyes96.272yes
thermophileno96.866yes
glucose-utilyes88.985yes
flagellatedyes77.463no
glucose-fermentno93.986no

External links

@ref: 24337

culture collection no.: DSM 100124, KCTC 33758, MCC 2765, AS 8

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27031366Fictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range.Sharma A, Kohli P, Singh Y, Schumann P, Lal RInt J Syst Evol Microbiol10.1099/ijsem.0.0010512016Bacillaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA Gyrase/genetics, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny29802421Fictibacillus iocasae sp. nov., isolated from the deep-sea sediment in Pacmanus, Manus Basin.Wang HL, Zhang J, Sun LArch Microbiol10.1007/s00203-018-1527-x2018Bacillaceae/classification/genetics/*isolation & purification/metabolism, Base Composition, Cell Wall/metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/*microbiology, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/classification/genetics/metabolismMetabolism

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24337Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100124Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100124)
43636Anukriti Sharma, Puneet Kohli, Yogendra Singh, Peter Schumann, Rup LalFictibacillus halophilus sp. nov., from a microbial mat of a hot spring atop the Himalayan Range10.1099/ijsem.0.001051IJSEM 66: 2409-2416 201627031366
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1