Amycolatopsis sulphurea (DSM 46092, ATCC 27624, IFO 13270)

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

• @ref:	12433
  multimedia content:	DSM_46092-1.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_46092-1.jpg
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

• @ref:	12433
  multimedia content:	DSM_46092.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_46092.jpg
  caption:	Medium 83 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
12433	CZAPEK PEPTONE AGAR (DSMZ Medium 83)		Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water
12433	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20189	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20189	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20189	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20189	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20189	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20189	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

Oxygen tolerance

Compound production

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20189	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	+	hydrolysis	from API 20E
20189	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
20189	28757 ChEBI	fructose	+
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
20189	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68368	25094 ChEBI	lysine	+	degradation	from API 20E
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
20189	29864 ChEBI	mannitol	-
20189	17268 ChEBI	myo-inositol	-
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
20189	16634 ChEBI	raffinose	-
20189	26546 ChEBI	rhamnose	-
20189	17992 ChEBI	sucrose	-
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
20189	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
67770		Cetocycline
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	-		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68368	arginine dihydrolase	+	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	+		from API Coryne
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68368	lysine decarboxylase	+	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68379	urease	-	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	suberin monomers biosynthesis	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	L-lactaldehyde degradation	100	3 of 3
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	adipate degradation	100	2 of 2
66794	aerobactin biosynthesis	100	1 of 1
66794	cardiolipin biosynthesis	100	7 of 7
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	Entner Doudoroff pathway	100	10 of 10
66794	glycogen metabolism	100	5 of 5
66794	taurine degradation	100	1 of 1
66794	ethanol fermentation	100	2 of 2
66794	biotin biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	resorcinol degradation	100	2 of 2
66794	ketogluconate metabolism	100	8 of 8
66794	palmitate biosynthesis	100	22 of 22
66794	lipoate biosynthesis	100	5 of 5
66794	acetate fermentation	100	4 of 4
66794	ppGpp biosynthesis	100	4 of 4
66794	octane oxidation	100	3 of 3
66794	starch degradation	100	10 of 10
66794	valine metabolism	100	9 of 9
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	cellulose degradation	100	5 of 5
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	coenzyme A metabolism	100	4 of 4
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	vitamin K metabolism	100	5 of 5
66794	folate polyglutamylation	100	1 of 1
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	proline metabolism	90.91	10 of 11
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	vitamin B12 metabolism	88.24	30 of 34
66794	peptidoglycan biosynthesis	86.67	13 of 15
66794	citric acid cycle	85.71	12 of 14
66794	heme metabolism	85.71	12 of 14
66794	purine metabolism	85.11	80 of 94
66794	alanine metabolism	82.76	24 of 29
66794	glycolysis	82.35	14 of 17
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	myo-inositol biosynthesis	80	8 of 10
66794	factor 420 biosynthesis	80	4 of 5
66794	3-chlorocatechol degradation	80	4 of 5
66794	pyrimidine metabolism	80	36 of 45
66794	flavin biosynthesis	80	12 of 15
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	photosynthesis	78.57	11 of 14
66794	glutathione metabolism	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	allantoin degradation	77.78	7 of 9
66794	urea cycle	76.92	10 of 13
66794	non-pathway related	76.32	29 of 38
66794	tryptophan metabolism	76.32	29 of 38
66794	degradation of sugar acids	76	19 of 25
66794	histidine metabolism	75.86	22 of 29
66794	cyclohexanol degradation	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	methionine metabolism	73.08	19 of 26
66794	metabolism of disaccharids	72.73	8 of 11
66794	cysteine metabolism	72.22	13 of 18
66794	tyrosine metabolism	71.43	10 of 14
66794	propanol degradation	71.43	5 of 7
66794	lipid metabolism	70.97	22 of 31
66794	glycine metabolism	70	7 of 10
66794	vitamin B1 metabolism	69.23	9 of 13
66794	degradation of sugar alcohols	68.75	11 of 16
66794	oxidative phosphorylation	68.13	62 of 91
66794	acetoin degradation	66.67	2 of 3
66794	arginine metabolism	66.67	16 of 24
66794	lysine metabolism	66.67	28 of 42
66794	enterobactin biosynthesis	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	degradation of aromatic, nitrogen containing compounds	66.67	8 of 12
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	phenol degradation	65	13 of 20
66794	phenylpropanoid biosynthesis	61.54	8 of 13
66794	degradation of pentoses	60.71	17 of 28
66794	creatinine degradation	60	3 of 5
66794	carotenoid biosynthesis	59.09	13 of 22
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	aclacinomycin biosynthesis	57.14	4 of 7
66794	daunorubicin biosynthesis	55.56	5 of 9
66794	nitrate assimilation	55.56	5 of 9
66794	cholesterol biosynthesis	54.55	6 of 11
66794	vitamin B6 metabolism	54.55	6 of 11
66794	d-xylose degradation	54.55	6 of 11
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	sulfate reduction	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	androgen and estrogen metabolism	50	8 of 16
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	butanoate fermentation	50	2 of 4
66794	toluene degradation	50	2 of 4
66794	ribulose monophosphate pathway	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	vitamin E metabolism	50	2 of 4
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	coenzyme M biosynthesis	50	5 of 10
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	selenocysteine biosynthesis	50	3 of 6
66794	polyamine pathway	47.83	11 of 23
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	lipid A biosynthesis	44.44	4 of 9
66794	4-hydroxymandelate degradation	44.44	4 of 9
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	ascorbate metabolism	40.91	9 of 22
66794	glycine betaine biosynthesis	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of hexoses	38.89	7 of 18
66794	carnitine metabolism	37.5	3 of 8
66794	arachidonic acid metabolism	33.33	6 of 18
66794	IAA biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	phosphatidylethanolamine bioynthesis	30.77	4 of 13
66794	mevalonate metabolism	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	methanogenesis from CO2	25	3 of 12
66794	catecholamine biosynthesis	25	1 of 4

API 20E

API coryne

API zym

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM256404v1 assembly for Amycolatopsis sulphurea DSM 46092	contig	GCA_002564045	76022.6	2630968289	76022	76.71

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Amycolatopsis sulphurea 16S ribosomal RNA gene, partial sequence	AF051343	1486		76022
20218	Amycolatopsis sulphurea partial 16S rRNA gene, strain IMSNU 20060T	AJ293756	1494		76022

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Identification of a novel mannose-capped lipoarabinomannan from Amycolatopsis sulphurea.	Gibson KJ, Gilleron M, Constant P, Puzo G, Nigou J, Besra GS.	Biochem J	10.1042/bj20030197	2003
	Surfactants tailored by the class Actinobacteria.	Kugler JH, Le Roes-Hill M, Syldatk C, Hausmann R.	Front Microbiol	10.3389/fmicb.2015.00212	2015
Enzymology	An Improved Transformation-Associated Recombination Cloning Approach for Direct Capturing of Natural Product Biosynthetic Gene Clusters.	Kurylenko O, Palusczak A, Luzhetskyy A, Rebets Y.	Microb Biotechnol	10.1111/1751-7915.70067	2024
	Heterologous expression of the atypical tetracycline chelocardin reveals the full set of genes required for its biosynthesis.	Lukezic T, Pikl S, Zaburannyi N, Remskar M, Petkovic H, Muller R.	Microb Cell Fact	10.1186/s12934-020-01495-x	2020
	Amidochelocardin Overcomes Resistance Mechanisms Exerted on Tetracyclines and Natural Chelocardin.	Hennessen F, Miethke M, Zaburannyi N, Loose M, Lukezic T, Bernecker S, Huttel S, Jansen R, Schmiedel J, Fritzenwanker M, Imirzalioglu C, Vogel J, Westermann AJ, Hesterkamp T, Stadler M, Wagenlehner F, Petkovic H, Herrmann J, Muller R.	Antibiotics (Basel)	10.3390/antibiotics9090619	2020
Enzymology	Heterologous Expression and Partial Characterization of a New Alanine Dehydrogenase from Amycolatopsis sulphurea.	Aktas F.	Protein J	10.1007/s10930-021-09982-9	2021
	Amycolatopsis sulphurea as the first reported agent of endophthalmitis in a patient after penetrating eye trauma with intraocular foreign body: A case report.	Yuce B, Dogan S, Ture G, Ozkalay N.	Indian J Ophthalmol	10.4103/ijo.ijo_399_20	2020
Metabolism	Engineering Atypical Tetracycline Formation in Amycolatopsis sulphurea for the Production of Modified Chelocardin Antibiotics.	Lukezic T, Fayad AA, Bader C, Harmrolfs K, Bartuli J, Gross S, Lesnik U, Hennessen F, Herrmann J, Pikl S, Petkovic H, Muller R.	ACS Chem Biol	10.1021/acschembio.8b01125	2019
Metabolism	Identification of the chelocardin biosynthetic gene cluster from Amycolatopsis sulphurea: a platform for producing novel tetracycline antibiotics.	Lukezic T, Lesnik U, Podgorsek A, Horvat J, Polak T, Sala M, Jenko B, Raspor P, Herron PR, Hunter IS, Petkovic H.	Microbiology (Reading)	10.1099/mic.0.070995-0	2013
	Rapid identification of atypical tetracyclines using tandem mass spectrometric fragmentation patterns.	Sala M, Kocar D, Lukezic T, Kosec G, Hodoscek M, Petkovic H.	Rapid Commun Mass Spectrom	10.1002/rcm.7252	2015
	In Vitro Activity of Two Novel Antimicrobial Compounds on MDR-Resistant Clinical Isolates.	Rima M, Pfennigwerth N, Cremanns M, Cirnski K, Oueslati S, Gatermann SG, d'Amelio N, Herrmann J, Muller R, Naas T.	Antibiotics (Basel)	10.3390/antibiotics12081265	2023
Phylogeny	Critical analysis of polycyclic tetramate macrolactam biosynthetic gene cluster phylogeny and functional diversity.	Harper CP, Day A, Tsingos M, Ding E, Zeng E, Stumpf SD, Qi Y, Robinson A, Greif J, Blodgett JAV.	Appl Environ Microbiol	10.1128/aem.00600-24	2024
	Metabolic engineering of a methyltransferase for production of drug precursors demecycline and demeclocycline in Streptomyces aureofaciens.	Yang W, Kong L, Wang Q, Deng Z, You D.	Synth Syst Biotechnol	10.1016/j.synbio.2020.06.001	2020
Biotechnology	Synthetic biology approaches to actinomycete strain improvement.	Breitling R, Avbelj M, Bilyk O, Carratore FD, Filisetti A, Hanko EKR, Iorio M, Redondo RP, Reyes F, Rudden M, Severi E, Slemc L, Schmidt K, Whittall DR, Donadio S, Garcia AR, Genilloud O, Kosec G, De Lucrezia D, Petkovic H, Thomas G, Takano E.	FEMS Microbiol Lett	10.1093/femsle/fnab060	2021
Enzymology	Epoxyquinomicins A, B, C and D, new antibiotics from Amycolatopsis. I. Taxonomy, fermentation, isolation and antimicrobial activities.	Matsumoto N, Tsuchida T, Umekita M, Kinoshita N, Iinuma H, Sawa T, Hamada M, Takeuchi T.	J Antibiot (Tokyo)	10.7164/antibiotics.50.900	1997
Phylogeny	Characterization of the ribosomal rrnD operon of the cephamycin-producer 'Nocardia lactamdurans' shows that this actinomycete belongs to the genus Amycolatopsis.	Barreiro C, Pisabarro A, Martin JF.	Syst Appl Microbiol	10.1016/s0723-2020(00)80041-7	2000
Metabolism	Azicemicins A and B, a new antimicrobial agent produced by Amycolatopsis. I. Taxonomy, fermentation, isolation, characterization and biological activities.	Tsuchida T, Inuma H, Kinoshita N, Ikeda T, Sawa T, Hamada M, Takeuchi T.	J Antibiot (Tokyo)	10.7164/antibiotics.48.217	1995
Enzymology	Characterization of a truncated lipoarabinomannan from the Actinomycete Turicella otitidis.	Gilleron M, Garton NJ, Nigou J, Brando T, Puzo G, Sutcliffe IC.	J Bacteriol	10.1128/jb.187.3.854-861.2005	2005
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Phylogeny	Amycolatopsis ponsaeliensis sp. nov., a novel endophytic actinobacterium isolated from the root nodules of Alnus glutinosa.	Thompson RM, Fox EM, Koutsidis G, Del Carmen Montero-Calasanz M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006810	2025
Phylogeny	Amycolatopsis ultiminotia sp. nov., isolated from rhizosphere soil, and emended description of the genus Amycolatopsis.	Lee SD.	Int J Syst Evol Microbiol	10.1099/ijs.0.006577-0	2009
Phylogeny	Amycolatopsis jejuensis sp. nov. and Amycolatopsis halotolerans sp. nov., novel actinomycetes isolated from a natural cave.	Lee SD.	Int J Syst Evol Microbiol	10.1099/ijs.0.63881-0	2006
Phylogeny	Amycolatopsis panacis sp. nov., isolated from Panax notoginseng rhizospheric soil.	Peng G, Xiong DS, Li LC, Hu JY, Bao S, Chen YW, Li YQ, Xu LH, Miao CP, Zhao LX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003202	2019
Phylogeny	Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine.	Tatar D, Sazak A, Guven K, Cetin D, Sahin N	Int J Syst Evol Microbiol	10.1099/ijs.0.050963-0	2013
Phylogeny	Amycolatopsis jiangsuensis sp. nov., a novel endophytic actinomycete isolated from a coastal plant in Jiangsu, China.	Xing K, Liu W, Zhang YJ, Bian GK, Zhang WD, Tamura T, Lee JS, Qin S, Jiang JH	Antonie Van Leeuwenhoek	10.1007/s10482-012-9823-y	2012