Name: Klebsiella electrica 1GB
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 132164

Type strain:

Species: Klebsiella electrica

Strain Designation: 1GB

Culture col. no.: DSM 102253, KCTC 32430, NBRC 109676

Strain history: <- NBRC <- S. Okabe, Division of Environmental Engineering, Hokkaido University, Sapporo, Japan; 1GB

NCBI tax ID(s): 1259973 (species)

SI-ID 401807

DSM 102253

Section

Klebsiella electrica 1GB is a mesophilic, Gram-negative bacterium that was isolated from anodic biofilm of glucose-fed microbial fuel cell originally inoculated with sewage sludge.

Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Klebsiella*
	Species	**Klebsiella electrica**
	Full Scientific Name (LPSN)	Klebsiella electrica (Kimura et al. 2014) Ma et al. 2022

Synonym

Raoultella electrica

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.888

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23953]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #23953]

Culture medium growth

[Ref.: #23953]

Culture medium link

Medium recipe at MediaDive

[Ref.: #23953]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #23953]			growth	28 °C

[Ref.: #23953]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.784

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #23953]	16883	+	-	+	-	+	-	+	-	-	+	-	+	+	+	+	-	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23953]	Sample type/isolated from	anodic biofilm of glucose-fed microbial fuel cell originally inoculated with sewage sludge
[Ref.: #23953]	Country	Japan
[Ref.: #23953]	Country ISO 3 Code	JPN
[Ref.: #23953]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Laboratory	#Lab enrichment
#Engineered	#Waste	#Sewage sludge
#Environmental	#Biofilm	-
#Environmental	#Microbial community	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB762091 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	20
Soil Samples	22
Animal Samples	107
Plant Samples	10
Total Samples	159

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #23953]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23953]	Raoultella electrica gene for 16S ribosomal RNA, partial sequence	AB762091	1452	ENA	1259973 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Raoultella electrica DSM 102253	GCA_006711645	complete	GenBank	1259973 tax ID	*
[Ref.: #66792]	Raoultella electrica strain DSM 102253	1259973.4	complete	PATRIC	1259973 tax ID	*
[Ref.: #66792]	Raoultella electrica DSM 102253	2887857370	complete	JGI IMG/M	1259973 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Raoultella electrica DSM 102253 NCBI: GCA_006711645.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Raoultella electrica DSM 102253 NCBI: GCA_006711645.1
[Ref.: #69480]	motile	no	80.859	no
[Ref.: #69480]	flagellated	no	93.45	no
[Ref.: #69480]	gram-positive	no	97.501	no
[Ref.: #69480]	anaerobic	no	98.082	no
[Ref.: #69480]	aerobic	yes	75.77	no
[Ref.: #69480]	halophile	no	91.699	no
[Ref.: #69480]	spore-forming	no	93.973	no
[Ref.: #69480]	thermophile	no	99.545	yes
[Ref.: #69480]	glucose-util	yes	93.655	no
[Ref.: #69480]	glucose-ferment	yes	87.915	yes

Availability in culture collections External links

[Ref.: #23953]

Culture collection no.

DSM 102253, KCTC 32430, NBRC 109676

[Ref.: #90520]

SI-ID 401807

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Raoultella electrica 1GB (DSM 102253(T)), Isolated from Anodic Biofilms of a Glucose-Fed Microbial Fuel Cell.	Thiel S, Bunk B, Sproer C, Overmann J, Jahn D, Biedendieck R	Microbiol Resour Announc	10.1128/MRA.00800-19	2019	*
Phylogeny	First report of isolation and antibiotic susceptibility pattern of Raoultella electrica from table eggs in Jaipur, India.	Jain AK, Yadav R	New Microbes New Infect	10.1016/j.nmni.2017.11.004	2017	*
Phylogeny	Raoultella electrica sp. nov., isolated from anodic biofilms of a glucose-fed microbial fuel cell.	Kimura ZI, Chung KM, Itoh H, Hiraishi A, Okabe S	Int J Syst Evol Microbiol	10.1099/ijs.0.058826-0	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23953

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 102253

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#90520

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401807.1 )

* These data were automatically processed and therefore are not curated

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Raoultella electrica DSM 102253

Raoultella electrica DSM 102253

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