Strain identifier
BacDive ID: 132164
Type strain: 
Species: Klebsiella electrica
Strain Designation: 1GB
Strain history: <- NBRC <- S. Okabe, Division of Environmental Engineering, Hokkaido University, Sapporo, Japan; 1GB
NCBI tax ID(s): 1259973 (species)
version 8.1 (current version)
General
@ref: 23953
BacDive-ID: 132164
DSM-Number: 102253
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative
description: Klebsiella electrica 1GB is a mesophilic, Gram-negative bacterium that was isolated from anodic biofilm of glucose-fed microbial fuel cell originally inoculated with sewage sludge.
NCBI tax id
- NCBI tax id: 1259973
- Matching level: species
strain history
- @ref: 23953
- history: <- NBRC <- S. Okabe, Division of Environmental Engineering, Hokkaido University, Sapporo, Japan; 1GB
doi: 10.13145/bacdive132164.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Enterobacterales
- family: Enterobacteriaceae
- genus: Klebsiella
- species: Klebsiella electrica
- full scientific name: Klebsiella electrica (Kimura et al. 2014) Ma et al. 2022
synonyms
- @ref: 20215
- synonym: Raoultella electrica
@ref: 23953
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Enterobacteriales, not validated
family: Enterobacteriaceae
genus: Raoultella
species: Raoultella electrica
full scientific name: Raoultella electrica Kimura et al. 2014
strain designation: 1GB
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.888
Culture and growth conditions
culture medium
- @ref: 23953
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
- @ref: 23953
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.784 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68368 | 30849 | L-arabinose | + | fermentation |
| 68368 | 27613 | amygdalin | + | fermentation |
| 68368 | 28053 | melibiose | + | fermentation |
| 68368 | 17992 | sucrose | + | fermentation |
| 68368 | 62345 | L-rhamnose | - | fermentation |
| 68368 | 30911 | sorbitol | + | fermentation |
| 68368 | 17268 | myo-inositol | + | fermentation |
| 68368 | 16899 | D-mannitol | + | fermentation |
| 68368 | 17634 | D-glucose | + | fermentation |
| 68368 | 5291 | gelatin | - | hydrolysis |
| 68368 | 27897 | tryptophan | - | energy source |
| 68368 | 16199 | urea | + | hydrolysis |
| 68368 | 16947 | citrate | + | assimilation |
| 68368 | 18257 | ornithine | - | degradation |
| 68368 | 25094 | lysine | + | degradation |
| 68368 | 29016 | arginine | - | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 68368 | 15688 | acetoin | yes |
| 68368 | 35581 | indole | no |
| 68368 | 16136 | hydrogen sulfide | no |
metabolite tests
| @ref | Chebi-ID | metabolite | voges-proskauer-test | indole test |
|---|---|---|---|---|
| 68368 | 15688 | acetoin | + | |
| 68368 | 35581 | indole | - |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68368 | cytochrome oxidase | - | 1.9.3.1 |
| 68368 | gelatinase | - | |
| 68368 | tryptophan deaminase | - | 4.1.99.1 |
| 68368 | urease | + | 3.5.1.5 |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 |
| 68368 | lysine decarboxylase | + | 4.1.1.18 |
| 68368 | arginine dihydrolase | - | 3.5.3.6 |
| 68368 | beta-galactosidase | + | 3.2.1.23 |
API 20E
| @ref | ONPG | ADH Arg | LDC Lys | ODC | CIT | H2S | URE | TDA Trp | IND | VP | GEL | GLU | MAN | INO | Sor | RHA | SAC | MEL | AMY | ARA | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 23953 | + | - | + | - | + | - | + | - | - | + | - | + | + | + | + | - | + | + | + | + | - |
Isolation, sampling and environmental information
isolation
- @ref: 23953
- sample type: anodic biofilm of glucose-fed microbial fuel cell originally inoculated with sewage sludge
- country: Japan
- origin.country: JPN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Laboratory | #Lab enrichment |
| #Engineered | #Waste | #Sewage sludge |
| #Environmental | #Biofilm | |
| #Environmental | #Microbial community |
taxonmaps
- @ref: 69479
- File name: preview.99_873.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_6;96_7;97_7;98_78;99_873&stattab=map
- Last taxonomy: Enterobacteriaceae
- 16S sequence: AB762091
- Sequence Identity:
- Total samples: 159
- soil counts: 22
- aquatic counts: 20
- animal counts: 107
- plant counts: 10
Safety information
risk assessment
- @ref: 23953
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 23953
- description: Raoultella electrica gene for 16S ribosomal RNA, partial sequence
- accession: AB762091
- length: 1452
- database: ena
- NCBI tax ID: 1259973
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Raoultella electrica DSM 102253 | GCA_006711645 | complete | ncbi | 1259973 |
| 66792 | Raoultella electrica strain DSM 102253 | 1259973.4 | complete | patric | 1259973 |
| 66792 | Raoultella electrica DSM 102253 | 2887857370 | complete | img | 1259973 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 80.859 | no |
| flagellated | no | 93.45 | no |
| gram-positive | no | 97.501 | no |
| anaerobic | no | 98.082 | no |
| aerobic | yes | 75.77 | no |
| halophile | no | 91.699 | no |
| spore-forming | no | 93.973 | no |
| thermophile | no | 99.545 | yes |
| glucose-util | yes | 93.655 | no |
| glucose-ferment | yes | 87.915 | yes |
External links
@ref: 23953
culture collection no.: DSM 102253, KCTC 32430, NBRC 109676
straininfo link
- @ref: 90520
- straininfo: 401807
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24449794 | Raoultella electrica sp. nov., isolated from anodic biofilms of a glucose-fed microbial fuel cell. | Kimura ZI, Chung KM, Itoh H, Hiraishi A, Okabe S | Int J Syst Evol Microbiol | 10.1099/ijs.0.058826-0 | 2014 | Bacterial Typing Techniques, Base Composition, Bioelectric Energy Sources/*microbiology, *Biofilms, Enterobacteriaceae/*classification/genetics/isolation & purification, Fatty Acids/chemistry, Genes, Bacterial, Glucose/metabolism, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry | Genetics |
| Phylogeny | 29379605 | First report of isolation and antibiotic susceptibility pattern of Raoultella electrica from table eggs in Jaipur, India. | Jain AK, Yadav R | New Microbes New Infect | 10.1016/j.nmni.2017.11.004 | 2017 | ||
| Genetics | 31649093 | Complete Genome Sequence of Raoultella electrica 1GB (DSM 102253(T)), Isolated from Anodic Biofilms of a Glucose-Fed Microbial Fuel Cell. | Thiel S, Bunk B, Sproer C, Overmann J, Jahn D, Biedendieck R | Microbiol Resour Announc | 10.1128/MRA.00800-19 | 2019 | Phylogeny |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 23953 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-102253 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102253) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 68368 | Automatically annotated from API 20E | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 90520 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID401807.1 |