Name: Actinosynnema pretiosum subsp. auranticum DSM 44131
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 13212

Type strain:

Species: Actinosynnema pretiosum subsp. auranticum

Culture col. no.: DSM 44131, ATCC 31309, FERM P-4130, IFO 13725, JCM 7343, NBRC 13725, RTCI C-14482, IFO 15620, IMSNU 21345, KCTC 9498, NBRC 15620, NCIMB 14387, NRRL B-16078, VKM Ac-1961

Strain history: ATCC 31309 <-- RTCI C-14482 (N-1001).

NCBI tax ID(s): 42198 (subspecies)

SI-ID 44893

ATCC 31309, IFO 13725, IFO 15620, DSM 44131, JCM 7343, NRRL B-16078, VKM Ac-1961, KCTC 9498, NBRC 13725, NBRC 15620

Special Collections

Section

Actinosynnema pretiosum subsp. auranticum DSM 44131 is a spore-forming bacterium that builds an aerial mycelium and was isolated from surface of blade of carex species.

spore-forming
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Actinosynnema*
	Species	**Actinosynnema pretiosum subsp. auranticum**
	Full Scientific Name (LPSN)	Actinosynnema pretiosum subsp. auranticum Hasegawa et al. 1983

Information on morphological and physiological properties Morphology

[Ref.: #69230]	Colony color	Daffodil yellow (1007), yellow orange (2000)
[Ref.: #69230]	Cultivation medium used	ISP 3

[Ref.: #69230]	Colony color	Daffodil yellow (1007), yellow orange (2000)
[Ref.: #69230]	Cultivation medium used	ISP 5

[Ref.: #69230]	Colony color	Daffodil yellow (1007), yellow orange (2000)
[Ref.: #69230]	Cultivation medium used	ISP 7

[Ref.: #69230]	Colony color	Daffodil yellow (1007)
[Ref.: #69230]	Cultivation medium used	ISP 2

[Ref.: #69230]	Colony color	Daffodil yellow (1007)
[Ref.: #69230]	Cultivation medium used	ISP 4

[Ref.: #69230]	Colony color	Daffodil yellow (1007)
[Ref.: #69230]	Cultivation medium used	ISP 6

[Ref.: #69230]	Colony color	Ochre brown (8001)
[Ref.: #69230]	Cultivation medium used	suter without tyrosine

[Ref.: #69230]	Colony color	Ochre brown (8001), mahogany brown (8016)
[Ref.: #69230]	Cultivation medium used	suter with tyrosine

[Ref.: #69230]	Pigment production	yes
[Ref.: #69230]	Pigment name	Melanin
[Ref.: #69230]	Pigment production	yes
[Ref.: #69230]	Pigmentation color	Daffodil yellow (1007), ochre brown (8001)
[Ref.: #69230]	Pigment name	soluble pigment

[Ref.: #69230]	Multicellular complex forming ability	no
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Medium Name (multicellularity)	ISP 2

[Ref.: #69230]	Multicellular complex forming ability	yes
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Multicellular complex color	Cream (9001)
[Ref.: #69230]	Medium Name (multicellularity)	ISP 3

[Ref.: #69230]	Multicellular complex forming ability	yes
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Multicellular complex color	Cream (9001)
[Ref.: #69230]	Medium Name (multicellularity)	ISP 4

[Ref.: #69230]	Multicellular complex forming ability	no
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Medium Name (multicellularity)	ISP 5

[Ref.: #69230]	Multicellular complex forming ability	no
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Medium Name (multicellularity)	ISP 6

[Ref.: #69230]	Multicellular complex forming ability	yes
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Multicellular complex color	Cream (9001)
[Ref.: #69230]	Medium Name (multicellularity)	ISP 7

[Ref.: #69230]	Multicellular complex forming ability	no
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69230]	Multicellular complex forming ability	no
[Ref.: #69230]	Multicellular complex name	Aerial mycelium
[Ref.: #69230]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #11490]

Caption:

Medium 65 28°C

[Ref.: #11490]

Intellectual property rights:

[Ref.: #69230]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69230]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69230]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69230]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11490]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11490]

Culture medium growth

[Ref.: #11490]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11490]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #11490]			growth	28 °C
[Ref.: #67770]			growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]

Ability of spore formation

yes Confidence in %100

[Ref.: #11490]	Name of produced compound	antibiotic C-14482 A
[Ref.: #11490]	Name of produced compound	antibiotic C-14482 B₁
[Ref.: #11490]	Name of produced compound	antibiotic C-14482 B₂
[Ref.: #11490]	Name of produced compound	antibiotic C-14482 B₃
[Ref.: #20216]	Name of produced compound	Dnacin A1
[Ref.: #20216]	Name of produced compound	Dnacin B1
[Ref.: #20216]	Name of produced compound	Vitamin B1
[Ref.: #20216]	Name of produced compound	Vitamin B2
[Ref.: #20216]	Name of produced compound	Vitamin B3

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69230]		NaCl		growth	2.5-5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69230]	arabinose ChEBI	+/-	growth
[Ref.: #69230]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69230]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69230]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69230]	inositol ChEBI	-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69230]	mannose ChEBI	+	growth
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #69230]	raffinose ChEBI	+/-	growth
[Ref.: #69230]	rhamnose ChEBI	+	growth
[Ref.: #69230]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69230]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69230]	3724	+	+	+	+	-	+	+	+	+	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69230]	5908	not determinedn.d.	+	+	+/-	-	+	+	-	+/-	+	+	+	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11490]	Sample type/isolated from	surface of blade of carex species
[Ref.: #11490]	Geographic location (country and/or sea, region)	Shiga Pref.
[Ref.: #11490]	Country	Japan
[Ref.: #11490]	Country ISO 3 Code	JPN
[Ref.: #11490]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Surface of blade of Carex species
[Ref.: #67770]	Host species	Carex
[Ref.: #67770]	Geographic location (country and/or sea, region)	Shiga Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB303364 (>99% sequence identity) for Actinosynnema from Microbeatlas

Aquatic Samples	2
Soil Samples	121
Animal Samples	30
Plant Samples	35
Total Samples	188

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11490]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinosynnema pretiosum subsp. auranticum gene for 16S rRNA, partial sequence	AB122775	564	GenBank ENA	42198 tax ID	*
[Ref.: #20218]	Actinosynnema pretiosum subsp. auranticum gene for 16S rRNA, partial sequence	AB303364	1472	GenBank ENA	42198 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinosynnema pretiosum subsp. auranticum NRRL B-16078	GCA_000716705	contig	GenBank ENA	42198 tax ID
[Ref.: #66792]	Actinosynnema pretiosum subsp. auranticum DSM 44131	GCA_013387285	contig	GenBank ENA	42198 tax ID	*
[Ref.: #66792]	Actinosynnema pretiosum subsp. auranticum strain DSM 44131	42198.10	wgs	PATRIC	42198 tax ID	*

[Ref.: #11490]	GC-content	71 mol%
[Ref.: #67770]	GC-content	70-72 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinosynnema pretiosum subsp. auranticum strain DSM 44131 PATRIC: 42198.10
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinosynnema pretiosum subsp. auranticum strain DSM 44131 PATRIC: 42198.10
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	85.704	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.383	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	87.254	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	88.148	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.4	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	60.5	no

Availability in culture collections External links

[Ref.: #11490]

Culture collection no.

DSM 44131, ATCC 31309, FERM P-4130, IFO 13725, JCM 7343, NBRC 13725, RTCI C-14482, IFO 15620, IMSNU 21345, KCTC 9498, NBRC 15620, NCIMB 14387, NRRL B-16078, VKM Ac-1961

[Ref.: #82420]

SI-ID 44893

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	p-Aminophenylalanine Involved in the Biosynthesis of Antitumor Dnacin B1 for Quinone Moiety Formation.	Hu X, Li X, Sheng Y, Wang H, Li X, Ou Y, Deng Z, Bai L, Kang Q	Molecules	10.3390/molecules25184186	2020	*

References

#11490

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44131

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69230

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82420

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44893.1 )

* These data were automatically processed and therefore are not curated

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Actinosynnema pretiosum subsp. auranticum strain DSM 44131

Actinosynnema pretiosum subsp. auranticum strain DSM 44131

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