Actinosynnema pretiosum subsp. auranticum (DSM 44131, ATCC 31309, FERM P-4130)

Name: Actinosynnema pretiosum subsp. auranticum (DSM 44131, ATCC 31309, FERM P-4130)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 13212

Type strain

Culture col. no. DSM 44131 ATCC 31309 FERM P-4130 IFO 13725 JCM 7343 13 more

NCBI tax ID(s) 42198

Special Collections DSMZ JCM KCTC Wink compendium JMRC

History DSMZ <- JCM <- ATCC <- RTCI C-14482 (N-1001) JCM <-- ATCC 31309 <-- RTCI C-14482 (N-1001).

Links

Actinosynnema pretiosum subsp. auranticum DSM 44131 is a bacterium that builds an aerial mycelium and was isolated from surface of blade of carex species.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 44893

ATCC 31309,IFO 13725,IFO 15620,DSM 44131,JCM 7343,NRRL B-16078,VKM Ac-1961,KCTC 9498,NBRC 13725,NBRC 15620

@ref 20215

Last LPSN update 2026-05-29 08:27:59

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Pseudonocardiales

Family Pseudonocardiaceae

Genus Actinosynnema

Species Actinosynnema pretiosum subsp. auranticum

Full scientific name Actinosynnema pretiosum subsp. auranticum Hasegawa et al. 1983

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125439	positive	99.693
125439		94.194

Colony morphology

@ref	Colony color	Medium used
69230	Daffodil yellow (1007), yellow orange (2000)	ISP 3
69230	Daffodil yellow (1007), yellow orange (2000)	ISP 5
69230	Daffodil yellow (1007), yellow orange (2000)	ISP 7
69230	Daffodil yellow (1007)	ISP 2
69230	Daffodil yellow (1007)	ISP 4
69230	Daffodil yellow (1007)	ISP 6
69230	Ochre brown (8001)	suter without tyrosine
69230	Ochre brown (8001), mahogany brown (8016)	suter with tyrosine

Multicellular morphology

@ref	Complex name	Complex color	Medium name
69230	Aerial mycelium		ISP 2
69230	Aerial mycelium	Cream (9001)	ISP 3
69230	Aerial mycelium	Cream (9001)	ISP 4
69230	Aerial mycelium		ISP 5
69230	Aerial mycelium		ISP 6
69230	Aerial mycelium	Cream (9001)	ISP 7
69230	Aerial mycelium		suter with tyrosine
69230	Aerial mycelium		suter without tyrosine

Pigmentation

@ref	Production	Color	Name
69230			melanin pigment
69230		Daffodil yellow (1007), ochre brown (8001)	soluble pigment

Multimedia

@ref:	11490
multimedia content:	DSM_44131.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44131.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	69230
multimedia content:	DSM_44131_image3.jpeg
multimedia.multimedia content:	DSM_44131_image3.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

@ref:	69230
multimedia content:	DSM_44131_image4.jpeg
multimedia.multimedia content:	DSM_44131_image4.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
11490	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
11490	positive	growth	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.095

Spore formation

@ref	Spore formation	Confidence
125439		97.316

Compound production

@ref	Compound
11490	antibiotic C-14482 A
11490	antibiotic C-14482 B₁
11490	antibiotic C-14482 B₂
11490	antibiotic C-14482 B₃
20216	Dnacin A1
20216	Dnacin B1
20216	Vitamin B1
20216	Vitamin B2
20216	Vitamin B3

Halophily

@ref	Salt	Growth	Tested relation	Concentration
69230	NaCl	positive	growth	2.5-5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
69230	22599 ChEBI	arabinose	+/-	growth
69230	62968 ChEBI	cellulose	-	growth
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
69230	28757 ChEBI	fructose	+	growth
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
69230	17234 ChEBI	glucose	+	growth
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
69230	37684 ChEBI	mannose	+	growth
69230	17268 ChEBI	myo-inositol	-	growth
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
69230	16634 ChEBI	raffinose	+/-	growth
69230	26546 ChEBI	rhamnose	+	growth
69230	17992 ChEBI	sucrose	+	growth
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
69230	18222 ChEBI	xylose	+	growth

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68379	gelatinase	+		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69230	+	+	+	+	-	+	+	+	+	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69230	not determinedn.d.	+	+	+/-	-	+	+	-	+/-	+	+	+	+	+	-	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
11490	surface of blade of carex species		Shiga Pref.	Japan	JPN	Asia
67770	Surface of blade of Carex species	Carex	Shiga Pref.	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB303364 (>99% sequence identity) for Actinosynnema from Microbeatlas

Aquatic Samples	2
Soil Samples	121
Animal Samples	30
Plant Samples	35
Total Samples	188

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11490	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1338728v1 assembly for Actinosynnema pretiosum subsp. auranticum DSM 44131	contig	GCA_013387285	42198.10	8118075156	42198	76.02
67770	ASM71670v1 assembly for Actinosynnema pretiosum subsp. auranticum NRRL B-16078	contig	GCA_000716705	42198.3	2814123198	42198	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Actinosynnema pretiosum subsp. auranticum gene for 16S rRNA, partial sequence	AB122775	564		42198
20218	Actinosynnema pretiosum subsp. auranticum gene for 16S rRNA, partial sequence	AB303364	1472		42198

GC content

@ref	GC-content (mol%)
11490	71
67770	70-72

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1338728v1 assembly for Actinosynnema pretiosum subsp. auranticum DSM 44131
NCBI: GCA_013387285

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	positive	99.69	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.10	no
125439	motility	BacteriaNetⓘ	no	94.19	no
125439	spore_formation	BacteriaNetⓘ	yes	97.32	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinosynnema pretiosum subsp. auranticum strain DSM 44131
PATRIC: 42198.10

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	85.70	no
125438	anaerobic	anaerobicⓘ	no	96.38	no
125438	spore-forming	spore-formingⓘ	yes	87.25	no
125438	aerobic	aerobicⓘ	yes	88.15	no
125438	thermophilic	thermophileⓘ	no	95.40	yes
125438	flagellated	motile2+ⓘ	no	60.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Discovery and Characterization of Actinosynnelassin: An Anti-Pseudomonas fluorescens Lasso Peptide Derived from a Large Precursor Open Reading Frame.	Wen Y, Li Z, Ye J, Wang X, Jiang M, Deng Z, Cao C, He X.	J Nat Prod	10.1021/acs.jnatprod.5c00312	2025
	Increased yield of AP-3 by inactivation of asm25 in Actinosynnema pretiosum ssp. auranticum ATCC 31565.	Cheng H, Xiong G, Li Y, Zhu J, Xiong X, Wang Q, Zhang L, Dong H, Zhu C, Liu G, Chen H.	PLoS One	10.1371/journal.pone.0265517	2022
	Endowing homodimeric carbamoyltransferase GdmN with iterative functions through structural characterization and mechanistic studies.	Wei J, Zhang X, Zhou Y, Cheng X, Lin Z, Tang M, Zheng J, Wang B, Kang Q, Bai L.	Nat Commun	10.1038/s41467-022-34387-2	2022
Metabolism	The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum.	Yu TW, Bai L, Clade D, Hoffmann D, Toelzer S, Trinh KQ, Xu J, Moss SJ, Leistner E, Floss HG.	Proc Natl Acad Sci U S A	10.1073/pnas.092697199	2002
Metabolism	Efficient soluble expression of active recombinant human cyclin A2 mediated by E. coli molecular chaperones.	Grigoroudis AI, McInnes C, Premnath PN, Kontopidis G.	Protein Expr Purif	10.1016/j.pep.2015.01.013	2015
Metabolism	p-Aminophenylalanine Involved in the Biosynthesis of Antitumor Dnacin B1 for Quinone Moiety Formation.	Hu X, Li X, Sheng Y, Wang H, Li X, Ou Y, Deng Z, Bai L, Kang Q	Molecules	10.3390/molecules25184186	2020

References

#11490	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44131
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69230	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Actinosynnema pretiosum subsp. auranticum (DSM 44131, ATCC 31309, FERM P-4130)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_013387285

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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