Klebsiella oxytoca (DSM 29614, BAS-10)

Name: Klebsiella oxytoca (DSM 29614, BAS-10)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130303

Type strain

Strain Designation BAS-10

Culture col. no. DSM 29614 BAS-10

NCBI tax ID(s) 571

Special Collections DSMZ

History DSMZ <- F. Baldi, Cà Foscari Univ., Venezia, Italy; BAS-10 <- F. Baldi, Univ. Siena, Italy

Links

Klebsiella oxytoca BAS-10 is a human pathogen that produces polysaccharides and was isolated from acid drainages of pyrite mines, under an iron mat.

polysaccharide production human pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398057

DSM 29614

@ref 20215

Last LPSN update 2026-05-29 08:37:20

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Enterobacterales

Family Enterobacteriaceae

Genus Klebsiella

Species Klebsiella oxytoca

Full scientific name Klebsiella oxytoca (Flügge 1886) Lautrop 1956 (Approved Lists 1980)

Synonyms (1)

"Bacillus oxytocus"

BacDive ID	Other strains from **Klebsiella oxytoca** (44)	Type strain
4941	K. oxytoca 479-2, DSM 5175, ATCC 13182, IAM 14201, JCM ... (type strain)
130956	K. oxytoca 1028, DSM 24121
4942	K. oxytoca LRA 006 01 73, CCUG 29298, DSM 6673, ATCC ...
4943	K. oxytoca M5a1, DSM 7342, DSM 3539, NCIB 12204
4944	K. oxytoca TauN1, DSM 16963
4945	K. oxytoca Colworth2703, DSM 25736, CIP 110043, NCIMB ...
134506	K. oxytoca KER, CIP 105873
134796	K. oxytoca Sobeaut, CIP 66.6
134797	K. oxytoca Belloni, CIP 66.7
134983	K. oxytoca U.910.295092, CIP 107218
135290	K. oxytoca AmMS 101, CIP 103569, ATCC 49131
135330	K. oxytoca AmMS 247, CIP 103663, ATCC 49473
136480	K. oxytoca SL911, CIP 106098
136481	K. oxytoca SL9111, CIP 106099
137289	K. oxytoca SL 7811, CIP 105096
137590	K. oxytoca CIP 79.32
139515	K. oxytoca SL 781, CIP 104963
141247	K. oxytoca CCUG 383
142930	K. oxytoca CCUG 15788
143417	K. oxytoca CCUG 19048
143547	K. oxytoca CCUG 20014
143868	K. oxytoca CCUG 21666
144225	K. oxytoca CCUG 23732
144234	K. oxytoca CCUG 23880
144772	K. oxytoca CCUG 26509
144859	K. oxytoca CCUG 26858
145816	K. oxytoca CCUG 29683 A
146943	K. oxytoca CCUG 32516
147262	K. oxytoca CCUG 33222
150581	K. oxytoca CCUG 41654
150684	K. oxytoca CCUG 41957
150983	K. oxytoca CCUG 42935
151279	K. oxytoca CCUG 43602
153718	K. oxytoca CCUG 50735
153933	K. oxytoca CCUG 51683
155829	K. oxytoca CCUG 59412
155992	K. oxytoca CCUG 59935
156818	K. oxytoca CCUG 65544
173354	K. oxytoca U12, CRBIP19.318
173355	K. oxytoca U10, CRBIP19.319
173356	K. oxytoca CIP 111713
178762	K. oxytoca Hoechst 1522E, ST033702
178763	K. oxytoca Wiedemann IV3-23, ST033703
178764	K. oxytoca Hoechst 1082E, ST033704

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	97.457
125438	negative	100

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21611	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)		Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
21611	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)		Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

Culture temp

@ref	Growth	Type	Temperature (°C)
21611	positive	growth	30

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125439		99.326

Compound production

21611

Compoundpalladium-exopolysaccharide

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	27613 ChEBI	amygdalin	+	fermentation	from API 20E
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68368	16947 ChEBI	citrate	+	assimilation	from API 20E
68368	17634 ChEBI	D-glucose	+	fermentation	from API 20E
68368	16899 ChEBI	D-mannitol	+	fermentation	from API 20E
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
68368	30849 ChEBI	L-arabinose	+	fermentation	from API 20E
68368	62345 ChEBI	L-rhamnose	+	fermentation	from API 20E
68368	28053 ChEBI	melibiose	+	fermentation	from API 20E
68368	17268 ChEBI	myo-inositol	+	fermentation	from API 20E
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68368	30911 ChEBI	sorbitol	+	fermentation	from API 20E
68368	17992 ChEBI	sucrose	+	fermentation	from API 20E
68368	27897 ChEBI	tryptophan	+	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		+	from API 20E

Enzymes

@ref	Value	Activity	Ec
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
68368	gelatinase	-		from API 20E
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
21611	+	-	-	-	+	-	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
21611	+	-	+	-	+	-	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Other	#Mine
#Environmental	#Aquatic	-
#Environmental	#Biofilm	#Iron mat
#Condition	#Acidic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
21611	acid drainages of pyrite mines, under an iron mat	Southern Tuscany, Colline Metallifere	Italy	ITA	Europe	43.1833	10.9333 43.1833/10.9333

Taxonmaps

69479

Global distribution of 16S sequence KM881701 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	233
Soil Samples	121
Animal Samples	3440
Plant Samples	216
Total Samples	4010

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
21611			2	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM208010v1 assembly for Klebsiella oxytoca DSM 29614	scaffold	GCA_002080105	571.218	571	19.4

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21611	Klebsiella oxytoca strain BAS-10 16S ribosomal RNA gene, partial sequence	KM881701	1447		571

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM208010v1 assembly for Klebsiella oxytoca DSM 29614
NCBI: GCA_002080105

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	86.60	no
125439	gram_stain	BacteriaNetⓘ	negative	97.46	no
125439	motility	BacteriaNetⓘ	yes	69.54	no
125439	spore_formation	BacteriaNetⓘ	no	99.33	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Klebsiella oxytoca DSM 29614
NCBI: GCA_002080105.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	100.00	no
125438	anaerobic	anaerobicⓘ	no	96.75	no
125438	aerobic	aerobicⓘ	yes	51.44	no
125438	spore-forming	spore-formingⓘ	no	88.75	no
125438	thermophilic	thermophileⓘ	no	99.25	yes
125438	flagellated	motile2+ⓘ	no	75.52	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Genetic manipulations of nonmodel gut microbes.	Jin WB, Guo CJ.	Imeta	10.1002/imt2.216	2024
	Role of inorganic nanoparticle degradation in cancer therapy.	Maksoudian C, Saffarzadeh N, Hesemans E, Dekoning N, Buttiens K, Soenen SJ.	Nanoscale Adv	10.1039/d0na00286k	2020
	Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications.	Bhatnagar S, Kobori T, Ganesh D, Ogawa K, Aoyagi H.	Nanomaterials (Basel)	10.3390/nano9071042	2019
	Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation.	Banerjee A, Sarkar S, Govil T, Gonzalez-Faune P, Cabrera-Barjas G, Bandopadhyay R, Salem DR, Sani RK.	Front Microbiol	10.3389/fmicb.2021.721365	2021
Pathogenicity	A Current Overview of the Biological and Cellular Effects of Nanosilver.	Cameron SJ, Hosseinian F, Willmore WG.	Int J Mol Sci	10.3390/ijms19072030	2018
Metabolism	Biogenic iron-silver nanoparticles inhibit bacterial biofilm formation due to Ag(+) release as determined by a novel phycoerythrin-based assay.	Cusimano MG, Ardizzone F, Nasillo G, Gallo M, Sfriso A, Martino-Chillura D, Schillaci D, Baldi F, Gallo G	Appl Microbiol Biotechnol	10.1007/s00253-020-10686-w	2020
	Effects of biogenerated ferric hydroxides nanoparticles on truffle mycorrhized plants.	Leonardi P, Lugli F, Iotti M, Puliga F, Piana F, Gallo M, Baldi F, Vittori Antisari L, Zambonelli A, Chiarantini L	Mycorrhiza	10.1007/s00572-020-00947-x	2020
Genetics	Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages.	Gallo G, Presta L, Perrin E, Gallo M, Marchetto D, Puglia AM, Fani R, Baldi F	BMC Microbiol	10.1186/s12866-018-1330-5	2018
	Anticancer activity of biogenerated silver nanoparticles: an integrated proteomic investigation.	Buttacavoli M, Albanese NN, Di Cara G, Alduina R, Faleri C, Gallo M, Pizzolanti G, Gallo G, Feo S, Baldi F, Cancemi P	Oncotarget	10.18632/oncotarget.23859	2017
Pathogenicity	Bacteria-produced ferric exopolysaccharide nanoparticles as iron delivery system for truffles (Tuber borchii).	Picceri GG, Leonardi P, Iotti M, Gallo M, Baldi F, Zambonelli A, Amicucci A, Vallorani L, Piccoli G, Ciccimarra G, Arshakyan M, Burattini S, Falcieri E, Chiarantini L	Appl Microbiol Biotechnol	10.1007/s00253-017-8615-8	2017
Metabolism	An extracellular polymeric substance quickly chelates mercury(II) with N-heterocyclic groups.	Baldi F, Gallo M, Daniele S, Battistel D, Faleri C, Kodre A, Arcon I	Chemosphere	10.1016/j.chemosphere.2017.02.093	2017
Metabolism	Polysaccharide-based silver nanoparticles synthesized by Klebsiella oxytoca DSM 29614 cause DNA fragmentation in E. coli cells.	Baldi F, Daniele S, Gallo M, Paganelli S, Battistel D, Piccolo O, Faleri C, Puglia AM, Gallo G	Biometals	10.1007/s10534-016-9918-4	2016
Metabolism	XAS analysis of iron and palladium bonded to a polysaccharide produced anaerobically by a strain of Klebsiella oxytoca.	Arcon I, Paganelli S, Piccolo O, Gallo M, Vogel-Mikus K, Baldi F	J Synchrotron Radiat	10.1107/S1600577515010371	2015
	Aqueous biphasic treatment of some nitrocompounds with hydrogen in the presence of a biogenerated Pd-polysaccharide.	Paganelli S, Tassini R, La Sorella G, Piccolo O, Baldi F, Rathod VD	N Biotechnol	10.1016/j.nbt.2015.01.010	2015
Metabolism	Characterisation of biosynthesised silver nanoparticles by scanning electrochemical microscopy (SECM) and voltammetry.	Battistel D, Baldi F, Gallo M, Faleri C, Daniele S	Talanta	10.1016/j.talanta.2014.09.023	2014
Phylogeny	Adaptative biochemical pathways and regulatory networks in Klebsiella oxytoca BAS-10 producing a biotechnologically relevant exopolysaccharide during Fe(III)-citrate fermentation.	Gallo G, Baldi F, Renzone G, Gallo M, Cordaro A, Scaloni A, Puglia AM	Microb Cell Fact	10.1186/1475-2859-11-152	2012
Metabolism	Bio-generated metal binding polysaccharides as catalysts for synthetic applications and organic pollutant transformations.	Baldi F, Marchetto D, Paganelli S, Piccolo O	N Biotechnol	10.1016/j.nbt.2011.04.012	2011
Metabolism	Iron-binding characterization and polysaccharide production by Klebsiella oxytoca strain isolated from mine acid drainage.	Baldi F, Marchetto D, Battistel D, Daniele S, Faleri C, De Castro C, Lanzetta R	J Appl Microbiol	10.1111/j.1365-2672.2009.04302.x	2009
Enzymology	Leaching and microbial treatment of a soil contaminated by sulphide ore ashes and aromatic hydrocarbons.	D'Annibale A, Leonardi V, Federici E, Baldi F, Zecchini F, Petruccioli M	Appl Microbiol Biotechnol	10.1007/s00253-006-0749-z	2006
	Gel sequestration of heavy metals by Klebsiella oxytoca isolated from iron mat.	Baldi F, Minacci A, Pepi M, Scozzafava A	FEMS Microbiol Ecol	10.1111/j.1574-6941.2001.tb00837.x	2001

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21611	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29614
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68368	Automatically annotated from API 20E .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Klebsiella oxytoca (DSM 29614, BAS-10)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Compound production

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002080105

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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