Name: Klebsiella oxytoca BAS-10
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 130303

Type strain:

Species: Klebsiella oxytoca

Strain Designation: BAS-10

Culture col. no.: DSM 29614

Strain history: <- F. Baldi, Cà Foscari Univ., Venezia, Italy; BAS-10 <- F. Baldi, Univ. Siena, Italy

NCBI tax ID(s): 571 (species)

SI-ID 398057

DSM 29614

Other strains from Klebsiella oxytoca

K. oxytoca 479-2, DSM 5175, ATCC 13182, IAM ... (type strain)
K. oxytoca 1028, DSM 24121
K. oxytoca LRA 006 01 73, CCUG 29298, DSM ...
K. oxytoca M5a1, DSM 7342, DSM 3539, NCIB ...
K. oxytoca TauN1, DSM 16963
K. oxytoca Colworth2703, DSM 25736, CIP 110043, ...
K. oxytoca KER, CIP 105873
K. oxytoca Sobeaut, CIP 66.6
K. oxytoca Belloni, CIP 66.7
K. oxytoca U.910.295092, CIP 107218
K. oxytoca AmMS 101, CIP 103569, ATCC 49131
K. oxytoca AmMS 247, CIP 103663, ATCC 49473
K. oxytoca SL911, CIP 106098
K. oxytoca SL9111, CIP 106099
K. oxytoca SL 7811, CIP 105096
K. oxytoca CIP 79.32
K. oxytoca SL 781, CIP 104963
K. oxytoca CCUG 383
K. oxytoca CCUG 15788
K. oxytoca CCUG 19048
K. oxytoca CCUG 20014
K. oxytoca CCUG 21666
K. oxytoca CCUG 23732
K. oxytoca CCUG 23880
K. oxytoca CCUG 26509
K. oxytoca CCUG 26858
K. oxytoca CCUG 29683 A
K. oxytoca CCUG 32516
K. oxytoca CCUG 33222
K. oxytoca CCUG 41654
K. oxytoca CCUG 41957
K. oxytoca CCUG 42935
K. oxytoca CCUG 43602
K. oxytoca CCUG 50735
K. oxytoca CCUG 51683
K. oxytoca CCUG 59412
K. oxytoca CCUG 59935
K. oxytoca CCUG 65544
K. oxytoca U12, CRBIP19.318
K. oxytoca U10, CRBIP19.319
K. oxytoca CIP 111713

Section

Klebsiella oxytoca BAS-10 is a mesophilic, Gram-negative human pathogen that produces polysaccharides and was isolated from acid drainages of pyrite mines, under an iron mat.

polysaccharide production
Gram-negative
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Enterobacterales*
	Family	*Enterobacteriaceae*
	Genus	*Klebsiella*
	Species	**Klebsiella oxytoca**
	Full Scientific Name (LPSN)	Klebsiella oxytoca (Flügge 1886) Lautrop 1956 (Approved Lists 1980)

Synonym

"Bacillus oxytocus"

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.966

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21611]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #21611]

Culture medium growth

[Ref.: #21611]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #21611]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #21611]

Culture medium growth

[Ref.: #21611]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #21611]			growth	30 °C

[Ref.: #21611]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.741

[Ref.: #21611]

Name of produced compound

palladium-exopolysaccharide

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68368]	L-arabinose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	sorbitol ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	+	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	yes	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		+	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	+	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #21611]	18169	+	-	-	-	+	-	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.
[Ref.: #21611]	16730	+	-	+	-	+	-	-	-	+	+	-	+	+	+	+	+	+	+	+	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21611]	Sample type/isolated from	acid drainages of pyrite mines, under an iron mat
[Ref.: #21611]	Geographic location (country and/or sea, region)	Southern Tuscany, Colline Metallifere
[Ref.: #21611]	Country	Italy
[Ref.: #21611]	Country ISO 3 Code	ITA
[Ref.: #21611]	Continent	Europe
[Ref.: #21611]	Geographic location	43.1833°/10.9333°
* marker position based on {}

Isolation sources categories

#Engineered	#Other	#Mine
#Environmental	#Aquatic	-
#Environmental	#Biofilm	#Iron mat
#Condition	#Acidic	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KM881701 (>99% sequence identity) for Enterobacteriaceae from Microbeatlas

Aquatic Samples	233
Soil Samples	121
Animal Samples	3440
Plant Samples	216
Total Samples	4010

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21611]	Pathogenicity (human)	yes
[Ref.: #21611]	Pathogenicity (animal)	yes
[Ref.: #21611]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21611]	Klebsiella oxytoca strain BAS-10 16S ribosomal RNA gene, partial sequence	KM881701	1447	ENA	571 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Klebsiella oxytoca DSM 29614	GCA_002080105	scaffold	GenBank	571 tax ID	*
[Ref.: #66792]	Klebsiella oxytoca strain DSM 29614	571.218	wgs	PATRIC	571 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Klebsiella oxytoca strain DSM 29614 PATRIC: 571.218
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Klebsiella oxytoca DSM 29614 NCBI: GCA_002080105.1
[Ref.: #69480]	flagellated	no	93.949	no
[Ref.: #69480]	gram-positive	no	97.694	no
[Ref.: #69480]	anaerobic	no	96.403	no
[Ref.: #69480]	aerobic	yes	84.742	no
[Ref.: #69480]	halophile	no	89.352	no
[Ref.: #69480]	spore-forming	no	93.095	no
[Ref.: #69480]	glucose-util	yes	94.449	no
[Ref.: #69480]	thermophile	no	99.618	yes
[Ref.: #69480]	motile	no	81.099	no
[Ref.: #69480]	glucose-ferment	yes	87.047	yes

Availability in culture collections External links

[Ref.: #21611]

Culture collection no.

DSM 29614

[Ref.: #88838]

SI-ID 398057

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Biogenic iron-silver nanoparticles inhibit bacterial biofilm formation due to Ag(+) release as determined by a novel phycoerythrin-based assay.	Cusimano MG, Ardizzone F, Nasillo G, Gallo M, Sfriso A, Martino-Chillura D, Schillaci D, Baldi F, Gallo G	Appl Microbiol Biotechnol	10.1007/s00253-020-10686-w	2020	*
Genetics	Genomic traits of Klebsiella oxytoca DSM 29614, an uncommon metal-nanoparticle producer strain isolated from acid mine drainages.	Gallo G, Presta L, Perrin E, Gallo M, Marchetto D, Puglia AM, Fani R, Baldi F	BMC Microbiol	10.1186/s12866-018-1330-5	2018	*
Pathogenicity	Bacteria-produced ferric exopolysaccharide nanoparticles as iron delivery system for truffles (Tuber borchii).	Picceri GG, Leonardi P, Iotti M, Gallo M, Baldi F, Zambonelli A, Amicucci A, Vallorani L, Piccoli G, Ciccimarra G, Arshakyan M, Burattini S, Falcieri E, Chiarantini L	Appl Microbiol Biotechnol	10.1007/s00253-017-8615-8	2017	*
Metabolism	An extracellular polymeric substance quickly chelates mercury(II) with N-heterocyclic groups.	Baldi F, Gallo M, Daniele S, Battistel D, Faleri C, Kodre A, Arcon I	Chemosphere	10.1016/j.chemosphere.2017.02.093	2017	*
Metabolism	Polysaccharide-based silver nanoparticles synthesized by Klebsiella oxytoca DSM 29614 cause DNA fragmentation in E. coli cells.	Baldi F, Daniele S, Gallo M, Paganelli S, Battistel D, Piccolo O, Faleri C, Puglia AM, Gallo G	Biometals	10.1007/s10534-016-9918-4	2016	*
Metabolism	XAS analysis of iron and palladium bonded to a polysaccharide produced anaerobically by a strain of Klebsiella oxytoca.	Arcon I, Paganelli S, Piccolo O, Gallo M, Vogel-Mikus K, Baldi F	J Synchrotron Radiat	10.1107/S1600577515010371	2015	*
Metabolism	Characterisation of biosynthesised silver nanoparticles by scanning electrochemical microscopy (SECM) and voltammetry.	Battistel D, Baldi F, Gallo M, Faleri C, Daniele S	Talanta	10.1016/j.talanta.2014.09.023	2014	*
Phylogeny	Adaptative biochemical pathways and regulatory networks in Klebsiella oxytoca BAS-10 producing a biotechnologically relevant exopolysaccharide during Fe(III)-citrate fermentation.	Gallo G, Baldi F, Renzone G, Gallo M, Cordaro A, Scaloni A, Puglia AM	Microb Cell Fact	10.1186/1475-2859-11-152	2012	*
Metabolism	Bio-generated metal binding polysaccharides as catalysts for synthetic applications and organic pollutant transformations.	Baldi F, Marchetto D, Paganelli S, Piccolo O	N Biotechnol	10.1016/j.nbt.2011.04.012	2011	*
Metabolism	Iron-binding characterization and polysaccharide production by Klebsiella oxytoca strain isolated from mine acid drainage.	Baldi F, Marchetto D, Battistel D, Daniele S, Faleri C, De Castro C, Lanzetta R	J Appl Microbiol	10.1111/j.1365-2672.2009.04302.x	2009	*
Enzymology	Leaching and microbial treatment of a soil contaminated by sulphide ore ashes and aromatic hydrocarbons.	D'Annibale A, Leonardi V, Federici E, Baldi F, Zecchini F, Petruccioli M	Appl Microbiol Biotechnol	10.1007/s00253-006-0749-z	2006	*
	Effects of biogenerated ferric hydroxides nanoparticles on truffle mycorrhized plants.	Leonardi P, Lugli F, Iotti M, Puliga F, Piana F, Gallo M, Baldi F, Vittori Antisari L, Zambonelli A, Chiarantini L	Mycorrhiza	10.1007/s00572-020-00947-x	2020	*
	Anticancer activity of biogenerated silver nanoparticles: an integrated proteomic investigation.	Buttacavoli M, Albanese NN, Di Cara G, Alduina R, Faleri C, Gallo M, Pizzolanti G, Gallo G, Feo S, Baldi F, Cancemi P	Oncotarget	10.18632/oncotarget.23859	2017	*
	Aqueous biphasic treatment of some nitrocompounds with hydrogen in the presence of a biogenerated Pd-polysaccharide.	Paganelli S, Tassini R, La Sorella G, Piccolo O, Baldi F, Rathod VD	N Biotechnol	10.1016/j.nbt.2015.01.010	2015	*
	Gel sequestration of heavy metals by Klebsiella oxytoca isolated from iron mat.	Baldi F, Minacci A, Pepi M, Scozzafava A	FEMS Microbiol Ecol	10.1111/j.1574-6941.2001.tb00837.x	2001	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21611

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29614

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#88838

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398057.1 )

* These data were automatically processed and therefore are not curated

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Klebsiella oxytoca strain DSM 29614

Klebsiella oxytoca DSM 29614

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