Name: Myceligenerans crystallogenes CD12E2-27
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12589

Type strain:

Species: Myceligenerans crystallogenes

Strain Designation: CD12E2-27

Culture col. no.: DSM 17134, HKI 0369, NCIMB 14061, VTT E-032285, JCM 14326, NBRC 110697

Strain history: I. Groth CD12E2-27 (=HKI 0369).

NCBI tax ID(s): 316335 (species)

SI-ID 289865

DSM 17134, NCIMB 14061, VTT E-032285, JCM 14326

Special Collections

Section

Myceligenerans crystallogenes CD12E2-27 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from tufa, Catacomb Domitilla.

Gram-positive
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Promicromonosporaceae*
	Genus	*Myceligenerans*
	Species	**Myceligenerans crystallogenes**
	Full Scientific Name (LPSN)	Myceligenerans crystallogenes Groth et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #31535]	Gram stain	positive

[Ref.: #31535]	Cell width	0.5-0.7 µm

[Ref.: #31535]	Cell shape	rod-shaped

[Ref.: #31535]	Motility	no

[Ref.: #31535]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6781]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #6781]

Culture medium growth

[Ref.: #6781]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6781]

Culture medium composition

expand / minimize

[Ref.: #6781]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #6781]

Culture medium growth

[Ref.: #6781]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6781]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #6781]	growth	28 °C
[Ref.: #31535]	growth	10-40 °C
[Ref.: #31535]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #31535]			optimum	06-09
[Ref.: #31535]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31535]

Oxygen tolerance

aerobe

[Ref.: #31535]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31535]		NaCl		growth	0-5	%
[Ref.: #31535]		NaCl		optimum	0-5	%

[Ref.: #31535]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-9(H₈), MK-9(H₄), MK-9(H₆), MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31535]	galactose ChEBI	+	carbon source
[Ref.: #31535]	glucose ChEBI	+	carbon source
[Ref.: #31535]	mannose ChEBI	+	carbon source

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6781]	Sample type/isolated from	tufa, Catacomb Domitilla
[Ref.: #6781]	Geographic location (country and/or sea, region)	Rome
[Ref.: #6781]	Country	Italy
[Ref.: #6781]	Country ISO 3 Code	ITA
[Ref.: #6781]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Tufa from the catacomb of Domitilla
[Ref.: #67770]	Geographic location (country and/or sea, region)	Rome
[Ref.: #67770]	Country	Italy
[Ref.: #67770]	Country ISO 3 Code	ITA
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	-
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR733716 (>99% sequence identity) for Myceligenerans crystallogenes subclade from Microbeatlas

Aquatic Samples	8
Soil Samples	18
Animal Samples	40
Plant Samples	2
Total Samples	68

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6781]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6781]	Myceligenerans crystallogenes strain CD12E2-27 16S ribosomal RNA gene, partial sequence	AY928181	1044	GenBank ENA	316335 tax ID
[Ref.: #20218]	Myceligenerans crystallogenes partial 16S rRNA gene, type strain DSM17134T	FR733716	1469	GenBank ENA	316335 tax ID	*

[Ref.: #6781]	GC-content	72.3 mol%
[Ref.: #31535]	GC-content	72 mol%
[Ref.: #67770]	GC-content	72.3 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #6781]

Culture collection no.

DSM 17134, HKI 0369, NCIMB 14061, VTT E-032285, JCM 14326, NBRC 110697

[Ref.: #81803]

SI-ID 289865

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Antribacter gilvus gen. nov., sp. nov., a new member of the family Promicromonosporaceae from a karstic cavern.	Zhang LY, Fang BZ, Jiao JY, Zhang XT, Liu L, Meng XL, Ming H, Nie GX, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003682	2019	*
Phylogeny	Myceligenerans cantabricum sp. nov., a barotolerant actinobacterium isolated from a deep cold-water coral.	Sarmiento-Vizcaino A, Gonzalez V, Brana AF, Molina A, Acuna JL, Garcia LA, Blanco G	Int J Syst Evol Microbiol	10.1099/ijs.0.000107	2015	*
Phylogeny	Myceligenerans salitolerans sp. nov., a halotolerant actinomycete isolated from a salt lake in Xinjiang, China.	Guan TW, Wu N, Tang SK, Che ZM, Chen XG	Extremophiles	10.1007/s00792-012-0502-8	2012	*
Phylogeny	Myceligenerans halotolerans sp. nov., an actinomycete isolated from a salt lake, and emended description of the genus Myceligenerans.	Wang Y, Tang SK, Li Z, Lou K, Mao PH, Jin X, Klenk HP, Zhang LX, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.024091-0	2010	*
Phylogeny	Myceligenerans crystallogenes sp. nov., isolated from Roman catacombs.	Groth I, Schumann P, Schutze B, Gonzalez JM, Laiz L, Suihko ML, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.63756-0	2006	*

References

#6781

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17134

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#31535

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27828 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#81803

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID289865.1 )

#27828

IJSEM 283 2006 ( DOI 10.1099/ijs.0.63756-0 , PubMed 16403899 )

* These data were automatically processed and therefore are not curated

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