Name: Anaerosphaera aminiphila WN036
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 11852

Type strain:

Species: Anaerosphaera aminiphila

Strain Designation: WN036

Culture col. no.: DSM 21120, JCM 15094, KCTC 15145

Strain history: A. Ueki WN036.

NCBI tax ID(s): 1120995 (strain), 1120994 (species)

SI-ID 404547

DSM 21120

Special Collections

Section

Anaerosphaera aminiphila WN036 is an anaerobe, spore-forming, Gram-positive bacterium that was isolated from Methanogenic fermenter of cattle waste.

coccus-shaped
Gram-positive
spore-forming
anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Peptoniphilaceae*
	Genus	*Anaerosphaera*
	Species	**Anaerosphaera aminiphila**
	Full Scientific Name (LPSN)	Anaerosphaera aminiphila Ueki et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #29377]	Gram stain	positive

[Ref.: #29377]	Cell width	0.8 µm

[Ref.: #29377]	Cell shape	coccus-shaped

[Ref.: #29377]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15559]

Culture medium

PY + X MEDIUM (DSMZ Medium 104b)

[Ref.: #15559]

Culture medium growth

[Ref.: #15559]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15559]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15559]	growth	30 °C
[Ref.: #29377]	growth	10-37 °C
[Ref.: #29377]	optimum	35-37 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #29377]			optimum	6.7
[Ref.: #29377]			growth	5.8-7.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #15559]	Oxygen tolerance	anaerobe
[Ref.: #29377]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %91.209

[Ref.: #29377]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29377]		NaCl		growth	0-4	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29377]	acetate ChEBI	+	carbon source
[Ref.: #29377]	alanine ChEBI	+	carbon source
[Ref.: #29377]	arginine ChEBI	+	carbon source
[Ref.: #29377]	aspartate ChEBI	+	carbon source
[Ref.: #29377]	esculin ChEBI	+	hydrolysis
[Ref.: #29377]	glutamate ChEBI	+	carbon source
[Ref.: #29377]	glycine ChEBI	+	carbon source
[Ref.: #29377]	histidine ChEBI	+	carbon source
[Ref.: #29377]	propionate ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #29377]		hydrogen sulfide ChEBI	yes

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	threonine metabolism	100	10 of 10
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	palmitate biosynthesis	90.91	20 of 22
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	ethylmalonyl-CoA pathway	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	CO2 fixation in Crenarchaeota	77.78	7 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	chorismate metabolism	77.78	7 of 9
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	isoleucine metabolism	75	6 of 8
[Ref.: #66794]	gluconeogenesis	75	6 of 8
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	propanol degradation	71.43	5 of 7
[Ref.: #66794]	cardiolipin biosynthesis	71.43	5 of 7
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	leucine metabolism	69.23	9 of 13
[Ref.: #66794]	NAD metabolism	66.67	12 of 18
[Ref.: #66794]	molybdenum cofactor biosynthesis	66.67	6 of 9
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	pyrimidine metabolism	66.67	30 of 45
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	purine metabolism	65.96	62 of 94
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	62.5	5 of 8
[Ref.: #66794]	degradation of sugar alcohols	62.5	10 of 16
[Ref.: #66794]	alanine metabolism	62.07	18 of 29
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	phenylacetate degradation (aerobic)	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	methionine metabolism	57.69	15 of 26
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	glutathione metabolism	57.14	8 of 14
[Ref.: #66794]	cysteine metabolism	55.56	10 of 18
[Ref.: #66794]	histidine metabolism	55.17	16 of 29
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	urea cycle	53.85	7 of 13
[Ref.: #66794]	oxidative phosphorylation	53.85	49 of 91
[Ref.: #66794]	degradation of pentoses	50	14 of 28
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	starch degradation	50	5 of 10
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	non-pathway related	50	19 of 38
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	allantoin degradation	44.44	4 of 9
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	reductive acetyl coenzyme A pathway	42.86	3 of 7
[Ref.: #66794]	heme metabolism	42.86	6 of 14
[Ref.: #66794]	lysine metabolism	42.86	18 of 42
[Ref.: #66794]	tryptophan metabolism	42.11	16 of 38
[Ref.: #66794]	lipid metabolism	41.94	13 of 31
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	propionate fermentation	40	4 of 10
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	degradation of sugar acids	40	10 of 25
[Ref.: #66794]	phenylpropanoid biosynthesis	38.46	5 of 13
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	35.71	5 of 14
[Ref.: #66794]	isoprenoid biosynthesis	34.62	9 of 26
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	phenol degradation	30	6 of 20
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	ubiquinone biosynthesis	28.57	2 of 7
[Ref.: #66794]	metabolism of disaccharids	27.27	3 of 11
[Ref.: #66794]	3-phenylpropionate degradation	26.67	4 of 15
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	23.53	4 of 17
[Ref.: #66794]	ascorbate metabolism	22.73	5 of 22
[Ref.: #66794]	4-hydroxymandelate degradation	22.22	2 of 9
[Ref.: #66794]	lipid A biosynthesis	22.22	2 of 9
[Ref.: #66794]	polyamine pathway	21.74	5 of 23
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15559]	Sample type/isolated from	Methanogenic fermenter of cattle waste
[Ref.: #15559]	Geographic location (country and/or sea, region)	Hokkaido
[Ref.: #15559]	Country	Japan
[Ref.: #15559]	Country ISO 3 Code	JPN
[Ref.: #15559]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Methanogenic reactor treating cattle waste
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB298735 (>99% sequence identity) for Anaerosphaera aminiphila subclade from Microbeatlas

Aquatic Samples	13
Soil Samples	24
Animal Samples	145
Plant Samples	8
Total Samples	190

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15559]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15559]	Anaerosphaera aminiphila DSM 21120 gene for 16S rRNA, partial sequence	AB298735	1472	GenBank ENA	1120995 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Anaerosphaera aminiphila DSM 21120	GCA_900129925	contig	GenBank ENA	1120995 tax ID
[Ref.: #66792]	Anaerosphaera aminiphila DSM 21120	1120995.4	wgs	PATRIC	1120995 tax ID	*
[Ref.: #66792]	Anaerosphaera aminiphila DSM 21120	2585428066	draft	JGI IMG/M	1120995 tax ID	*

[Ref.: #15559]	GC-content	32.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #29377]	GC-content	32.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Anaerosphaera aminiphila DSM 21120 PATRIC: 1120995.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Anaerosphaera aminiphila DSM 21120 PATRIC: 1120995.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	82.079	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	91.209	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	65.126	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	97.054	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	89.307	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	86.643	yes

Availability in culture collections External links

[Ref.: #15559]

Culture collection no.

DSM 21120, JCM 15094, KCTC 15145

[Ref.: #81070]

SI-ID 404547

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequence of Anaerosphaera sp. Strain GS7-6-2, a Coccal Bacterium Isolated from a Biogas-Related Environment.	Rettenmaier R, Neuhaus K, Liebl W, Zverlov VV	Microbiol Resour Announc	10.1128/MRA.00205-19	2019	*
Phylogeny	Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste.	Ueki A, Abe K, Suzuki D, Kaku N, Watanabe K, Ueki K	Int J Syst Evol Microbiol	10.1099/ijs.0.011858-0	2009	*

References

#15559

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21120

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#29377

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25785 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#81070

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404547.1 )

#25785

IJSEM 3161 2009 ( DOI 10.1099/ijs.0.011858-0 , PubMed 19643871 )

* These data were automatically processed and therefore are not curated

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Anaerosphaera aminiphila DSM 21120

Anaerosphaera aminiphila DSM 21120

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