Anaerosphaera aminiphila (DSM 21120, JCM 15094, WN036)

Name: Anaerosphaera aminiphila (DSM 21120, JCM 15094, WN036)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 11852

Type strain

Strain Designation WN036

Culture col. no. DSM 21120 JCM 15094 WN036 KCTC 15145

NCBI tax ID(s) 1120995 1120994

Special Collections DSMZ JCM KCTC Literature strains

History <- A. Ueki, Yamagata Univ., Tsuruoka, Japan; WN036 <- A. Ueki {2005} A. Ueki WN036.

Links

Anaerosphaera aminiphila WN036 is an anaerobe, spore-forming, Gram-positive bacterium that was isolated from Methanogenic fermenter of cattle waste.

spore-forming Gram-positive coccus-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 404547

JCM 15094,DSM 21120

@ref 20215

Last LPSN update 2026-02-09 11:20:40

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Tissierellales

Family Peptoniphilaceae

Genus Anaerosphaera

Species Anaerosphaera aminiphila

Full scientific name Anaerosphaera aminiphila Ueki et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell width	Cell shape	Motility
29377	positive	0.8 µm	coccus-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
15559	PY + X MEDIUM (DSMZ Medium 104b)		Medium recipe at MediaDive	Name: PY + X MEDIUM (DSMZ Medium 104b; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na glutamate 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Pyridoxine hydrochloride 0.0003 g/l Nicotinic acid 0.0002 g/l Thiamine-HCl x 2 H2O 0.0002 g/l Vitamin B12 0.0001 g/l Calcium pantothenate 0.0001 g/l p-Aminobenzoic acid 8e-05 g/l D-(+)-biotin 2e-05 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
15559	positive	growth	30
29377	positive	growth	10-37
29377	positive	optimum	35-37
67770	positive	growth	30

Culture pH

@ref	Ability	Type	PH
29377	positive	optimum	6.7
29377	positive	growth	5.8-7.8

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
15559	anaerobe
29377	anaerobe
125438	anaerobe	91.209
125439	anaerobe	97.5

Spore formation

29377

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29377	NaCl	positive	growth	0-4 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29377	30089 ChEBI	acetate	+	carbon source
29377	16449 ChEBI	alanine	+	carbon source
29377	29016 ChEBI	arginine	+	carbon source
29377	35391 ChEBI	aspartate	+	carbon source
29377	4853 ChEBI	esculin	+	hydrolysis
29377	29987 ChEBI	glutamate	+	carbon source
29377	15428 ChEBI	glycine	+	carbon source
29377	27570 ChEBI	histidine	+	carbon source
29377	17272 ChEBI	propionate	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
29377	16136 ChEBI	hydrogen sulfide

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	coenzyme A metabolism	100	4 of 4
66794	threonine metabolism	100	10 of 10
66794	adipate degradation	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	palmitate biosynthesis	90.91	20 of 22
66794	peptidoglycan biosynthesis	80	12 of 15
66794	cellulose degradation	80	4 of 5
66794	Entner Doudoroff pathway	80	8 of 10
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	photosynthesis	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	chorismate metabolism	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	phenylalanine metabolism	76.92	10 of 13
66794	ppGpp biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	pentose phosphate pathway	72.73	8 of 11
66794	propanol degradation	71.43	5 of 7
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	leucine metabolism	69.23	9 of 13
66794	NAD metabolism	66.67	12 of 18
66794	molybdenum cofactor biosynthesis	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	pyrimidine metabolism	66.67	30 of 45
66794	acetoin degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	purine metabolism	65.96	62 of 94
66794	glutamate and glutamine metabolism	64.29	18 of 28
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	degradation of sugar alcohols	62.5	10 of 16
66794	alanine metabolism	62.07	18 of 29
66794	factor 420 biosynthesis	60	3 of 5
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	methylglyoxal degradation	60	3 of 5
66794	hydrogen production	60	3 of 5
66794	methionine metabolism	57.69	15 of 26
66794	citric acid cycle	57.14	8 of 14
66794	glutathione metabolism	57.14	8 of 14
66794	cysteine metabolism	55.56	10 of 18
66794	histidine metabolism	55.17	16 of 29
66794	proline metabolism	54.55	6 of 11
66794	urea cycle	53.85	7 of 13
66794	oxidative phosphorylation	53.85	49 of 91
66794	degradation of pentoses	50	14 of 28
66794	glycogen biosynthesis	50	2 of 4
66794	starch degradation	50	5 of 10
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	selenocysteine biosynthesis	50	3 of 6
66794	ethanol fermentation	50	1 of 2
66794	non-pathway related	50	19 of 38
66794	arginine metabolism	50	12 of 24
66794	ribulose monophosphate pathway	50	1 of 2
66794	ketogluconate metabolism	50	4 of 8
66794	quinate degradation	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	allantoin degradation	44.44	4 of 9
66794	d-mannose degradation	44.44	4 of 9
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	heme metabolism	42.86	6 of 14
66794	lysine metabolism	42.86	18 of 42
66794	tryptophan metabolism	42.11	16 of 38
66794	lipid metabolism	41.94	13 of 31
66794	myo-inositol biosynthesis	40	4 of 10
66794	glycine metabolism	40	4 of 10
66794	coenzyme M biosynthesis	40	4 of 10
66794	glycogen metabolism	40	2 of 5
66794	propionate fermentation	40	4 of 10
66794	arachidonate biosynthesis	40	2 of 5
66794	degradation of sugar acids	40	10 of 25
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	d-xylose degradation	36.36	4 of 11
66794	tyrosine metabolism	35.71	5 of 14
66794	tetrahydrofolate metabolism	35.71	5 of 14
66794	isoprenoid biosynthesis	34.62	9 of 26
66794	degradation of hexoses	33.33	6 of 18
66794	nitrate assimilation	33.33	3 of 9
66794	IAA biosynthesis	33.33	1 of 3
66794	enterobactin biosynthesis	33.33	1 of 3
66794	octane oxidation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	sulfate reduction	30.77	4 of 13
66794	phenol degradation	30	6 of 20
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	ubiquinone biosynthesis	28.57	2 of 7
66794	metabolism of disaccharids	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	biotin biosynthesis	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	bile acid biosynthesis, neutral pathway	23.53	4 of 17
66794	ascorbate metabolism	22.73	5 of 22
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	lipid A biosynthesis	22.22	2 of 9
66794	polyamine pathway	21.74	5 of 23

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15559	Methanogenic fermenter of cattle waste	Hokkaido	Japan	JPN	Asia
67770	Methanogenic reactor treating cattle waste

Taxonmaps

69479

Global distribution of 16S sequence AB298735 (>99% sequence identity) for Anaerosphaera aminiphila subclade from Microbeatlas

Aquatic Samples	13
Soil Samples	24
Animal Samples	145
Plant Samples	8
Total Samples	190

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15559	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2585428066 annotated assembly for Anaerosphaera aminiphila DSM 21120	contig	GCA_900129925	1120995.4	2585428066	1120995	72.52

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15559	Anaerosphaera aminiphila DSM 21120 gene for 16S rRNA, partial sequence	AB298735	1472		1120995

GC content

@ref	GC-content (mol%)	Method
15559	32.5	high performance liquid chromatography (HPLC)
29377	32.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Anaerosphaera aminiphila DSM 21120
NCBI: GCA_900129925

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	62.00	no
125439	motility	BacteriaNetⓘ	yes	73.80	no
125439	gram_stain	BacteriaNetⓘ	positive	69.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	97.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Anaerosphaera aminiphila DSM 21120
PATRIC: 1120995.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	82.08	no
125438	anaerobic	anaerobicⓘ	yes	91.21	yes
125438	spore-forming	spore-formingⓘ	no	65.13	no
125438	aerobic	aerobicⓘ	no	97.05	yes
125438	thermophilic	thermophileⓘ	no	89.31	no
125438	flagellated	motile2+ⓘ	no	86.64	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft genome sequences of two bacterial type strains isolated from a methanogenic bioreactor: Ruminiclostridium sufflavum DSM 19573^T and Anaerosphaera aminiphila DSM 21120^T.	Cusack J, Del Rio I, Spring S, Kyrpides N, Ivanova N, Seshadri R, Escobar M.	Microbiol Resour Announc	10.1128/mra.00578-25	2025
Genetics	Draft Genome Sequence of Anaerosphaera sp. Strain GS7-6-2, a Coccal Bacterium Isolated from a Biogas-Related Environment.	Rettenmaier R, Neuhaus K, Liebl W, Zverlov VV	Microbiol Resour Announc	10.1128/MRA.00205-19	2019
Phylogeny	Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste.	Ueki A, Abe K, Suzuki D, Kaku N, Watanabe K, Ueki K	Int J Syst Evol Microbiol	10.1099/ijs.0.011858-0	2009

References

#15559	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21120
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25785	IJSEM 3161 2009 ( DOI 10.1099/ijs.0.011858-0 , PubMed 19643871 )
#29377	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25785
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Anaerosphaera aminiphila (DSM 21120, JCM 15094, WN036)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900129925

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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