Strain identifier

BacDive ID: 11852

Type strain: Yes

Species: Anaerosphaera aminiphila

Strain Designation: WN036

Strain history: A. Ueki WN036.

NCBI tax ID(s): 1120995 (strain), 1120994 (species)

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General

@ref: 15559

BacDive-ID: 11852

DSM-Number: 21120

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, Gram-positive, coccus-shaped

description: Anaerosphaera aminiphila WN036 is an anaerobe, spore-forming, Gram-positive bacterium that was isolated from Methanogenic fermenter of cattle waste.

NCBI tax id

NCBI tax idMatching level
1120994species
1120995strain

strain history

@refhistory
15559<- A. Ueki, Yamagata Univ., Tsuruoka, Japan; WN036 <- A. Ueki {2005}
67770A. Ueki WN036.

doi: 10.13145/bacdive11852.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Anaerosphaera
  • species: Anaerosphaera aminiphila
  • full scientific name: Anaerosphaera aminiphila Ueki et al. 2009

@ref: 15559

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptoniphilaceae

genus: Anaerosphaera

species: Anaerosphaera aminiphila

full scientific name: Anaerosphaera aminiphila Ueki et al. 2009

strain designation: WN036

type strain: yes

Morphology

cell morphology

  • @ref: 29377
  • gram stain: positive
  • cell width: 0.8 µm
  • cell shape: coccus-shaped
  • motility: no

Culture and growth conditions

culture medium

  • @ref: 15559
  • name: PY + X MEDIUM (DSMZ Medium 104b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104b
  • composition: Name: PY + X MEDIUM (DSMZ Medium 104b; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na glutamate 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Pyridoxine hydrochloride 0.0003 g/l Nicotinic acid 0.0002 g/l Thiamine-HCl x 2 H2O 0.0002 g/l Vitamin B12 0.0001 g/l Calcium pantothenate 0.0001 g/l p-Aminobenzoic acid 8e-05 g/l D-(+)-biotin 2e-05 g/l Distilled water

culture temp

@refgrowthtypetemperature
15559positivegrowth30
29377positivegrowth10-37
29377positiveoptimum35-37
67770positivegrowth30

culture pH

@refabilitytypepH
29377positivegrowth5.8-7.8
29377positiveoptimum6.7

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
15559anaerobe
29377anaerobe
69480anaerobe91.209

spore formation

@refspore formationconfidence
29377yes
69481yes100

halophily

  • @ref: 29377
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-4 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2937730089acetate+carbon source
2937716449alanine+carbon source
2937729016arginine+carbon source
2937735391aspartate+carbon source
2937729987glutamate+carbon source
2937715428glycine+carbon source
2937727570histidine+carbon source
2937717272propionate+carbon source
293774853esculin+hydrolysis

metabolite production

  • @ref: 29377
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15559Methanogenic fermenter of cattle wasteHokkaidoJapanJPNAsia
67770Methanogenic reactor treating cattle waste

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Gastrointestinal tract#Feces (Stool)
#Condition#Anoxic (anaerobic)

taxonmaps

  • @ref: 69479
  • File name: preview.99_150220.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_38;96_57083;97_73791;98_99287;99_150220&stattab=map
  • Last taxonomy: Anaerosphaera aminiphila subclade
  • 16S sequence: AB298735
  • Sequence Identity:
  • Total samples: 190
  • soil counts: 24
  • aquatic counts: 13
  • animal counts: 145
  • plant counts: 8

Safety information

risk assessment

  • @ref: 15559
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15559
  • description: Anaerosphaera aminiphila DSM 21120 gene for 16S rRNA, partial sequence
  • accession: AB298735
  • length: 1472
  • database: nuccore
  • NCBI tax ID: 1120995

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerosphaera aminiphila DSM 211201120995.4wgspatric1120995
66792Anaerosphaera aminiphila DSM 211202585428066draftimg1120995
67770Anaerosphaera aminiphila DSM 21120GCA_900129925contigncbi1120995

GC content

@refGC-contentmethod
1555932.5high performance liquid chromatography (HPLC)
2937732.5

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes82.079no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes91.209yes
69480spore-formingspore-formingAbility to form endo- or exosporesno65.126no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.054yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno89.307no
69480flagellatedmotile2+Ability to perform flagellated movementno86.643yes

External links

@ref: 15559

culture collection no.: DSM 21120, JCM 15094, KCTC 15145

straininfo link

  • @ref: 81070
  • straininfo: 404547

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19643871Anaerosphaera aminiphila gen. nov., sp. nov., a glutamate-degrading, Gram-positive anaerobic coccus isolated from a methanogenic reactor treating cattle waste.Ueki A, Abe K, Suzuki D, Kaku N, Watanabe K, Ueki KInt J Syst Evol Microbiol10.1099/ijs.0.011858-02009Anaerobiosis, Animals, Biodegradation, Environmental, Bioreactors/*microbiology, Cattle, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/chemistry/metabolism, Feces/microbiology, Glutamic Acid/*metabolism, Methanol/*metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Veillonellaceae/*classification/genetics/*isolation & purification/metabolism, Waste Products/*analysisMetabolism
Genetics31023818Draft Genome Sequence of Anaerosphaera sp. Strain GS7-6-2, a Coccal Bacterium Isolated from a Biogas-Related Environment.Rettenmaier R, Neuhaus K, Liebl W, Zverlov VVMicrobiol Resour Announc10.1128/MRA.00205-192019Phylogeny

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
15559Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21120)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21120
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29377Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2578528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
81070Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404547.1StrainInfo: A central database for resolving microbial strain identifiers