Name: Bacillus horti K 13
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1154

Type strain:

Species: Bacillus horti

Strain Designation: K 13, K13

Culture col. no.: DSM 12751, JCM 9943, ATCC 700778, CGMCC 1.3650, CIP 105746, KCTC 3838, LMG 18497

Strain history: CIP <- 1998, JCM <- I. Yumoto: strain K13

NCBI tax ID(s): 77523 (species)

SI-ID 12121

LMG 18497, DSM 12751, JCM 9943, ATCC 700778, CIP 105746, CCM 4902, NCIMB 13846, KCTC 3838, NRRL B-23350, NRRL B-23381, CGMCC 1.3650

Special Collections

Other strains from Bacillus horti

Section

Bacillus horti K 13 is an obligate aerobe, spore-forming, Gram-negative bacterium that was isolated from garden soil.

Gram-negative
motile
rod-shaped
spore-forming
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus horti**
	Full Scientific Name (LPSN)	Bacillus horti Yumoto et al. 1998

Information on morphological and physiological properties Morphology

[Ref.: #23305]	Gram stain	negative
[Ref.: #119974]	Gram stain	negative

[Ref.: #23305]	Cell length	1.5-6.0 µm

[Ref.: #23305]	Cell width	0.6-0.8 µm

[Ref.: #23305]	Cell shape	rod-shaped
[Ref.: #119974]	Cell shape	rod-shaped

[Ref.: #23305]	Motility	yes
[Ref.: #119974]	Motility	yes

[Ref.: #23305]	Flagellum arrangement	peritrichous

[Ref.: #23305]

Colony color

white

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4802]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #4802]

Culture medium growth

[Ref.: #4802]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4802]

Culture medium composition

expand / minimize

[Ref.: #35192]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #35192]

Culture medium growth

[Ref.: #35192]

Culture medium composition

expand / minimize

[Ref.: #119974]

Culture medium

CIP Medium 6

[Ref.: #119974]

Culture medium growth

[Ref.: #119974]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119974]

Culture medium

CIP Medium 291

[Ref.: #119974]

Culture medium growth

[Ref.: #119974]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4802]		growth	30 °C
[Ref.: #23305]	no	growth	55 °C
[Ref.: #23305]	no	growth	45 °C
[Ref.: #23305]		growth	15 °C
[Ref.: #23305]	no	growth	5 °C
[Ref.: #23305]	no	growth	50 °C
[Ref.: #23305]		growth	40 °C
[Ref.: #23305]	no	growth	10 °C
[Ref.: #35192]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #119974]		growth	30-37 °C
[Ref.: #119974]	no	growth	10 °C
[Ref.: #119974]	no	growth	45 °C
[Ref.: #119974]	no	growth	55 °C

	Kind of pH		pH
[Ref.: #23305]		optimum	8-10
[Ref.: #23305]		growth	7
[Ref.: #119974]	no	growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23305]	Oxygen tolerance	obligate aerobe
[Ref.: #119974]	Oxygen tolerance	obligate aerobe

[Ref.: #23305]	Spore description	subterminally located ellipsoidal spores
[Ref.: #23305]	Type of spore	endospore
[Ref.: #23305]	Ability of spore formation	yes

	Salt	Tested relation		Salt conc.
[Ref.: #23305]	NaCl		growth	10	%
[Ref.: #119974]	NaCl	no	growth	2	%
[Ref.: #119974]	NaCl	no	growth	0	%
[Ref.: #119974]	NaCl		growth	6-10	%
[Ref.: #23305]	NaCl	no	growth	20	%
[Ref.: #23305]	NaCl	no	growth	18	%
[Ref.: #23305]	NaCl	no	growth	17	%
[Ref.: #23305]	NaCl	no	growth	16	%
[Ref.: #23305]	NaCl	no	growth	15	%
[Ref.: #23305]	NaCl	no	growth	14	%
[Ref.: #23305]	NaCl	no	growth	13	%
[Ref.: #23305]	NaCl	no	growth	12	%
[Ref.: #23305]	NaCl		growth	11	%
[Ref.: #23305]	NaCl		growth	9	%
[Ref.: #23305]	NaCl		growth	7	%
[Ref.: #23305]	NaCl		growth	3	%
[Ref.: #23305]	NaCl		growth	5	%
[Ref.: #119974]	NaCl	no	growth	4	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23305]	arabinose ChEBI	-	builds acid from
[Ref.: #23305]	casein	+	hydrolysis
[Ref.: #119974]	citrate ChEBI	-	carbon source
[Ref.: #23305]	D-fructose ChEBI	-	builds gas from
[Ref.: #23305]	D-fructose ChEBI	+	builds acid from
[Ref.: #23305]	D-galactose ChEBI	-	builds acid from
[Ref.: #23305]	D-glucose ChEBI	-	builds gas from
[Ref.: #23305]	D-glucose ChEBI	+	builds acid from
[Ref.: #23305]	D-ribose ChEBI	-	builds gas from
[Ref.: #23305]	D-ribose ChEBI	+	builds acid from
[Ref.: #23305]	D-xylose ChEBI	-	builds gas from
[Ref.: #23305]	D-xylose ChEBI	+	builds acid from
[Ref.: #23305]	dna ChEBI	+	hydrolysis
[Ref.: #23305]	erythritol ChEBI	-	builds acid from
[Ref.: #119974]	esculin ChEBI	-	hydrolysis
[Ref.: #23305]	gelatin ChEBI	+	hydrolysis
[Ref.: #23305]	hippurate ChEBI	+	hydrolysis
[Ref.: #119974]	hippurate ChEBI	+	hydrolysis
[Ref.: #23305]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23305]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23305]	nitrate ChEBI	+	reduction
[Ref.: #119974]	nitrate ChEBI	+	reduction
[Ref.: #119974]	nitrite ChEBI	-	reduction
[Ref.: #23305]	phenylalanine ChEBI	-	assimilation
[Ref.: #23305]	starch ChEBI	+	hydrolysis
[Ref.: #23305]	sucrose ChEBI	-	builds acid from
[Ref.: #23305]	tween 20 ChEBI	-	hydrolysis
[Ref.: #23305]	tween 40 ChEBI	-	hydrolysis
[Ref.: #23305]	tween 60 ChEBI	-	hydrolysis
[Ref.: #23305]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23305]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23305]		indole ChEBI	no
[Ref.: #119974]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #119974]		acetoin ChEBI	-
[Ref.: #119974]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #119974]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119974]	amylase	+
[Ref.: #23305]	beta-galactosidase	+	3.2.1.23
[Ref.: #119974]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119974]	caseinase	+	3.4.21.50
[Ref.: #23305]	catalase	+	1.11.1.6
[Ref.: #119974]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #23305]	cytochrome oxidase	+	1.9.3.1
[Ref.: #119974]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119974]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #119974]	gelatinase	+
[Ref.: #119974]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119974]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119974]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119974]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119974]	oxidase	+
[Ref.: #119974]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119974]	tryptophan deaminase	-
[Ref.: #119974]	tween esterase	-
[Ref.: #119974]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #119974]	1350	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119974]	8961	-	+	+	+	-	+	-	-	-	+	+	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4802]	Sample type/isolated from	garden soil
[Ref.: #4802]	Geographic location (country and/or sea, region)	Hokkaido, Astuma
[Ref.: #4802]	Country	Japan
[Ref.: #4802]	Country ISO 3 Code	JPN
[Ref.: #4802]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Atsuma, Hokkaido
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia

[Ref.: #119974]	Sample type/isolated from	Environment, Soil
[Ref.: #119974]	Country	Japan
[Ref.: #119974]	Country ISO 3 Code	JPN
[Ref.: #119974]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4802]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119974]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23305]	Bacillus horti gene for 16S rRNA, partial sequence	D87035	1512	GenBank ENA	77523 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus horti DSM 12751	2926514369	draft	JGI IMG/M	77523 tax ID	*

[Ref.: #23305]	GC-content	40.9 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	40 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #4802]

Culture collection no.

DSM 12751, JCM 9943, ATCC 700778, CGMCC 1.3650, CIP 105746, KCTC 3838, LMG 18497

[Ref.: #70825]

SI-ID 12121

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus horti sp. nov., a new gram-negative alkaliphilic bacillus.	Yumoto I, Yamazaki K, Sawabe T, Nakano K, Kawasaki K, Ezura Y, Shinano H	Int J Syst Bacteriol	10.1099/00207713-48-2-565	1998	*

References

#4802

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12751

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23305

Isao Yumoto, Koji Yamazaki, Tomoo Sawabe, Kazuaki Nakano, Kosei Kawasaki, Yoshio Ezura, Haruo Shinano: Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillus. IJSEM 48: 565 - 571 1998 ( DOI 10.1099/00207713-48-2-565 , PubMed 9731298 )

#35192 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#70825

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12121.1 )

#119974 Collection of Institut Pasteur ; Curators of the CIP; CIP 105746
* These data were automatically processed and therefore are not curated

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