Name: Bacillus horti K 13
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1154

Type strain:

Species: Bacillus horti

Strain Designation: K 13

Culture col. no.: DSM 12751, JCM 9943, ATCC 700778, CGMCC 1.3650, CIP 105746, KCTC 3838, LMG 18497

Strain history: I. Yumoto K13.

NCBI tax ID(s): 77523 (species)

Other strains from Bacillus horti

B. horti K 38, DSM 12752

Section

Bacillus horti K 13 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from garden soil.

Gram-negative
motile
rod-shaped
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus horti**
	Full Scientific Name (PNU)	Bacillus horti Yumoto et al. 1998

Information on morphological and physiological properties Morphology

[Ref.: #23305]	Gram stain	negative
[Ref.: #23305]	Cell length	1.5-6.0 µm
[Ref.: #23305]	Cell width	0.6-0.8 µm
[Ref.: #23305]	Cell shape	rod-shaped
[Ref.: #23305]	Motility	yes
[Ref.: #23305]	Flagellum arrangement	peritrichous

[Ref.: #23305]

Colony color

white

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4802]

Culture medium

ALKALINE NUTRIENT AGAR (DSMZ Medium 31)

[Ref.: #4802]

Culture medium growth

[Ref.: #4802]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4802]

Culture medium composition

expand / minimize

[Ref.: #35192]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #35192]

Culture medium growth

[Ref.: #35192]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #4802]		growth	30 °C
[Ref.: #23305]	no	growth	55 °C
[Ref.: #23305]	no	growth	45 °C
[Ref.: #23305]		growth	15 °C
[Ref.: #23305]	no	growth	5 °C
[Ref.: #23305]	no	growth	50 °C
[Ref.: #23305]		growth	40 °C
[Ref.: #23305]	no	growth	10 °C
[Ref.: #35192]		growth	30 °C
[Ref.: #67770]		growth	30 °C

[Ref.: #4802]	Temperature range	mesophilic
[Ref.: #23305]	Temperature range	thermophilic
[Ref.: #23305]	Temperature range	psychrophilic
[Ref.: #23305]	Temperature range	thermophilic
[Ref.: #23305]	Temperature range	psychrophilic
[Ref.: #35192]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #23305]			optimum	8-10	alkaliphile
[Ref.: #23305]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23305]

Oxygen tolerance

obligate aerobe

[Ref.: #23305]	Spore description	subterminally located ellipsoidal spores
[Ref.: #23305]	Type of spore	endospore
[Ref.: #23305]	Ability of spore formation	yes

	Salt	Tested relation		Salt conc.
[Ref.: #23305]	NaCl		growth	10	%
[Ref.: #23305]	NaCl		growth	5	%
[Ref.: #23305]	NaCl		growth	3	%
[Ref.: #23305]	NaCl		growth	7	%
[Ref.: #23305]	NaCl		growth	9	%
[Ref.: #23305]	NaCl		growth	11	%
[Ref.: #23305]	NaCl	no	growth	12	%
[Ref.: #23305]	NaCl	no	growth	13	%
[Ref.: #23305]	NaCl	no	growth	14	%
[Ref.: #23305]	NaCl	no	growth	15	%
[Ref.: #23305]	NaCl	no	growth	16	%
[Ref.: #23305]	NaCl	no	growth	17	%
[Ref.: #23305]	NaCl	no	growth	18	%
[Ref.: #23305]	NaCl	no	growth	20	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23305]	arabinose ChEBI	-	builds acid from
[Ref.: #23305]	casein	+	hydrolysis
[Ref.: #23305]	D-fructose ChEBI	-	builds gas from
[Ref.: #23305]	D-fructose ChEBI	+	builds acid from
[Ref.: #23305]	D-galactose ChEBI	-	builds acid from
[Ref.: #23305]	D-glucose ChEBI	-	builds gas from
[Ref.: #23305]	D-glucose ChEBI	+	builds acid from
[Ref.: #23305]	D-ribose ChEBI	-	builds gas from
[Ref.: #23305]	D-ribose ChEBI	+	builds acid from
[Ref.: #23305]	D-xylose ChEBI	-	builds gas from
[Ref.: #23305]	D-xylose ChEBI	+	builds acid from
[Ref.: #23305]	dna ChEBI	+	hydrolysis
[Ref.: #23305]	erythritol ChEBI	-	builds acid from
[Ref.: #23305]	gelatin ChEBI	+	hydrolysis
[Ref.: #23305]	hippurate ChEBI	+	hydrolysis
[Ref.: #23305]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23305]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23305]	nitrate ChEBI	+	reduction
[Ref.: #23305]	phenylalanine ChEBI	-	assimilation
[Ref.: #23305]	starch ChEBI	+	hydrolysis
[Ref.: #23305]	sucrose ChEBI	-	builds acid from
[Ref.: #23305]	tween 20 ChEBI	-	hydrolysis
[Ref.: #23305]	tween 40 ChEBI	-	hydrolysis
[Ref.: #23305]	tween 60 ChEBI	-	hydrolysis
[Ref.: #23305]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23305]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #23305]		indole ChEBI

	Enzyme	Enzyme activity	EC number
[Ref.: #23305]	beta-galactosidase	+	3.2.1.23
[Ref.: #23305]	catalase	+	1.11.1.6
[Ref.: #23305]	cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4802]	Sample type/isolated from	garden soil
[Ref.: #4802]	Geographic location (country and/or sea, region)	Hokkaido, Astuma
[Ref.: #4802]	Country	Japan
[Ref.: #4802]	Country ISO 3 Code	JPN
[Ref.: #4802]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Atsuma, Hokkaido
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	#Garden

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4802]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23305]	Bacillus horti gene for 16S rRNA, partial sequence	D87035	1512	GenBank	77523 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus horti DSM 12751	2926514369	draft	JGI IMG/M	77523 tax ID	*

[Ref.: #23305]	GC-content	40.9 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	40 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #4802]

Culture collection no.

DSM 12751, JCM 9943, ATCC 700778, CGMCC 1.3650, CIP 105746, KCTC 3838, LMG 18497

[Ref.: #20218]

Associated Passport(s) in StrainInfo

46034, 46035

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus horti sp. nov., a new gram-negative alkaliphilic bacillus.	Yumoto I, Yamazaki K, Sawabe T, Nakano K, Kawasaki K, Ezura Y, Shinano H	Int J Syst Bacteriol	10.1099/00207713-48-2-565	1998	*

References

#4802

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 12751

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23305

Isao Yumoto, Koji Yamazaki, Tomoo Sawabe, Kazuaki Nakano, Kosei Kawasaki, Yoshio Ezura, Haruo Shinano: Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillus. IJSEM 48: 565 - 571 1998 ( DOI 10.1099/00207713-48-2-565 , PubMed 9731298 )

#35192 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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