Name: Bacillus mycoides DSM 11821
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1153

Type strain:

Species: Bacillus mycoides

Culture col. no.: DSM 11821, CCM 4872, CCUG 58725, LMG 18989, CIP 105772, KCTC 3975, WSBC 10204

Strain history: <- R. Mayr

NCBI tax ID(s): 1405 (species)

Other strains from Bacillus mycoides

Section

Bacillus mycoides DSM 11821 is a facultative anaerobe, spore-forming, psychrophilic bacterium that was isolated from pasteurized milk.

Gram-positive
rod-shaped
spore-forming
facultative anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus mycoides**
	Full Scientific Name (PNU)	Bacillus mycoides Flügge 1886 (Approved Lists 1980)

Synonym

Bacillus weihenstephanensis

Information on morphological and physiological properties Morphology

[Ref.: #23304]	Gram stain	positive
[Ref.: #23304]	Cell shape	rod-shaped

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %93.312

[Ref.: #61746]

Incubation period

1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #38580]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38580]

Culture medium growth

[Ref.: #38580]

Culture medium composition

expand / minimize

[Ref.: #4502]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #4502]

Culture medium growth

[Ref.: #4502]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4502]

Culture medium composition

expand / minimize

[Ref.: #4502]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #4502]

Culture medium growth

[Ref.: #4502]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #4502]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #4502]		growth	30 °C
[Ref.: #23304]	no	growth	7 °C
[Ref.: #23304]	no	growth	43 °C
[Ref.: #23304]		growth	4 °C
[Ref.: #23304]		growth	10 °C
[Ref.: #23304]	no	growth	40 °C
[Ref.: #38580]		growth	30 °C
[Ref.: #61746]		growth	30-37 °C

[Ref.: #4502]	Temperature range	mesophilic
[Ref.: #23304]	Temperature range	psychrophilic
[Ref.: #23304]	Temperature range	thermophilic
[Ref.: #23304]	Temperature range	psychrophilic
[Ref.: #23304]	Temperature range	thermophilic
[Ref.: #38580]	Temperature range	mesophilic
[Ref.: #61746]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23304]	Oxygen tolerance	facultative anaerobe
[Ref.: #23304]	Oxygen tolerance	aerobe
[Ref.: #61746]	Oxygen tolerance	aerobe

[Ref.: #23304]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite
[Ref.: #68368]	acetoin ChEBI	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	* from API 20E
[Ref.: #68368]	indole ChEBI	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #61746]	5869	-	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #61746]	3297	-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4502]	Sample type/isolated from	pasteurized milk
[Ref.: #4502]	Geographic location (country and/or sea, region)	Bawaria
[Ref.: #4502]	Country	Germany
[Ref.: #4502]	Country ISO 3 Code	DEU
[Ref.: #4502]	Continent	Europe

[Ref.: #61746]	Sample type/isolated from	Pasteurized milk
[Ref.: #61746]	Sampling date	1992
[Ref.: #61746]	Country	Germany
[Ref.: #61746]	Country ISO 3 Code	DEU
[Ref.: #61746]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Dairy product
#Engineered	#Treatment	#Preserved
#Engineered	#Treatment	#Sterilized (Desinfected)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM747230 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	446
Soil Samples	1920
Animal Samples	1524
Plant Samples	1079
Total Samples	4969

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4502]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4502]	Bacillus weihenstephanensis gene for 16S ribosomal RNA	AB021199	1531	ENA	1405 tax ID
[Ref.: #20218]	Bacillus weihenstephanensis partial 16S rRNA gene and ITS1, strain DSM 11821	AJ841876	543	ENA	86662 tax ID	*
[Ref.: #20218]	Bacillus weihenstephanensis clone DSMZ1182R 16S ribosomal RNA gene, partial sequence	DQ290004	1418	ENA	86662 tax ID	*
[Ref.: #20218]	Bacillus weihenstephanensis gene for 16S rRNA, partial sequence, strain: NBRC 101238	AB681419	1477	ENA	86662 tax ID	*
[Ref.: #20218]	Bacillus weihenstephanensis partial 16S rRNA gene and ITS1, strain WSBC 10204	AM747230	1663	ENA	86662 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus mycoides NBRC 101238 = DSM 11821 NBRC 101238	GCA_000513155	contig	GenBank	1220585 tax ID	*
[Ref.: #66792]	Bacillus weihenstephanensis NBRC 101238 = DSM 11821	1220585.4	wgs	PATRIC	1220585 tax ID	*
[Ref.: #66792]	Bacillus weihenstephanensis strain WSBC 10204	86662.17	complete	PATRIC	1405 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus weihenstephanensis strain WSBC 10204 PATRIC: 86662.17
[Ref.: #69480]	gram-positive	yes	100	no
[Ref.: #69480]	anaerobic	no	98.934	no
[Ref.: #69480]	spore-forming	yes	100	yes
[Ref.: #69480]	thermophile	no	99.686	no
[Ref.: #69480]	motile	yes	93.312	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus weihenstephanensis strain WSBC 10204 PATRIC: 86662.17
[Ref.: #69481]	spore-forming	yes	100	no

Availability in culture collections External links

[Ref.: #4502]

Culture collection no.

DSM 11821, CCM 4872, CCUG 58725, LMG 18989, CIP 105772, KCTC 3975, WSBC 10204

[Ref.: #20218]

Associated Passport(s) in StrainInfo

48746, 162892

Literature:

Topic	Title	Authors	Journal	DOI	Year
Stress	Bacillus weihenstephanensis can readily evolve for increased endospore heat resistance without compromising its thermotype.	Kim TD, Begyn K, Khanal S, Taghlaoui F, Heyndrickx M, Rajkovic A, Devlieghere F, Michiels C, Aertsen A	Int J Food Microbiol	10.1016/j.ijfoodmicro.2021.109072	2021	*
Metabolism	Temperature Dependence of the Proteome Profile of the Psychrotolerant Pathogenic Food Spoiler Bacillus weihenstephanensis Type Strain WSBC 10204.	Stelder SK, Mahmud SA, Dekker HL, de Koning LJ, Brul S, de Koster CG	J Proteome Res	10.1021/pr501307t	2015	*
Phylogeny	Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group.	Lechner S, Mayr R, Francis KP, Pruss BM, Kaplan T, Wiessner-Gunkel E, Stewart GS, Scherer S	Int J Syst Bacteriol	10.1099/00207713-48-4-1373	1998	*

References

#4502

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11821

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23304

SABINE LECHNER, RALF MAYR, KEVIN P. FRANCIS, BIRGIT M. PRüß, THOMAS KAPLAN, ELKE WIEßNER-GUNKEL, GORDON S. A. B. STEWART, SIEGFRIED SCHERER: Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. IJSEM 48: 1373 - 1382 1998 ( DOI 10.1099/00207713-48-4-1373 , PubMed 9828439 )

#38580 ; Curators of the CIP;
#61746 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58725

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Bacillus weihenstephanensis strain WSBC 10204

Bacillus weihenstephanensis strain WSBC 10204

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