Name: Bacillus mycoides DSM 11821
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1153

Type strain:

Species: Bacillus mycoides

Culture col. no.: DSM 11821, CCM 4872, CCUG 58725, LMG 18989, CIP 105772, KCTC 3975, WSBC 10204, WDCM 00222, WS 2480

Strain history: CIP <- 1998, R. Mayr, FML Techn. Munich Univ., Freising, Germany, Bacillus weihenstephanensis

NCBI tax ID(s): 1405 (species)

SI-ID 14497

LMG 18989, DSM 11821, CIP 105772, CCM 4872, CECT 5894, NRRL B-23307, KCTC 3975, NBRC 101238, BCRC 17605, CCRC 17605, VTT E-001721, CCUG 58725

Other strains from Bacillus mycoides

Section

Bacillus mycoides DSM 11821 is a facultative anaerobe, spore-forming, psychrophilic bacterium that was isolated from pasteurized milk.

Gram-positive
rod-shaped
spore-forming
facultative anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus mycoides**
	Full Scientific Name (LPSN)	Bacillus mycoides Flügge 1886 (Approved Lists 1980)

Synonym

Bacillus weihenstephanensis

Information on morphological and physiological properties Morphology

[Ref.: #23304]	Gram stain	positive
[Ref.: #121209]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #23304]	Cell shape	rod-shaped
[Ref.: #121209]	Cell shape	rod-shaped

[Ref.: #121209]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %93.312

[Ref.: #61746]

Incubation period

1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #38580]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #38580]

Culture medium growth

[Ref.: #38580]

Culture medium composition

expand / minimize

[Ref.: #4502]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #4502]

Culture medium growth

[Ref.: #4502]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4502]

Culture medium composition

expand / minimize

[Ref.: #4502]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #4502]

Culture medium growth

[Ref.: #4502]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4502]

Culture medium composition

expand / minimize

[Ref.: #121209]

Culture medium

CIP Medium 3

[Ref.: #121209]

Culture medium growth

[Ref.: #121209]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4502]		growth	30 °C
[Ref.: #23304]	no	growth	7 °C
[Ref.: #23304]	no	growth	40 °C
[Ref.: #23304]		growth	10 °C
[Ref.: #23304]		growth	4 °C
[Ref.: #23304]	no	growth	43 °C
[Ref.: #38580]		growth	30 °C
[Ref.: #61746]		growth	30-37 °C
[Ref.: #121209]		growth	10-37 °C
[Ref.: #121209]	no	growth	45 °C
[Ref.: #121209]	no	growth	55 °C

[Ref.: #4502]	Temperature range	mesophilic
[Ref.: #23304]	Temperature range	psychrophilic
[Ref.: #23304]	Temperature range	thermophilic
[Ref.: #23304]	Temperature range	psychrophilic
[Ref.: #23304]	Temperature range	thermophilic
[Ref.: #38580]	Temperature range	mesophilic
[Ref.: #61746]	Temperature range	mesophilic
[Ref.: #121209]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #121209]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23304]	Oxygen tolerance	facultative anaerobe
[Ref.: #23304]	Oxygen tolerance	aerobe
[Ref.: #61746]	Oxygen tolerance	aerobe
[Ref.: #121209]	Oxygen tolerance	facultative anaerobe

[Ref.: #23304]	Ability of spore formation	yes
[Ref.: #121209]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #121209]	NaCl		growth	0-4	%
[Ref.: #121209]	NaCl	no	growth	6	%
[Ref.: #121209]	NaCl	no	growth	8	%
[Ref.: #121209]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #121209]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121209]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #121209]	hippurate ChEBI	-	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #121209]	nitrate ChEBI	+	reduction
[Ref.: #121209]	nitrate ChEBI	+	respiration
[Ref.: #121209]	nitrite ChEBI	+	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #121209]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #121209]	acetoin ChEBI	+
[Ref.: #121209]	glucose ChEBI		+
[Ref.: #68368]	acetoin ChEBI	+			* from API 20E
[Ref.: #68368]	indole ChEBI			-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121209]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121209]	amylase	+
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #121209]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121209]	caseinase	+	3.4.21.50
[Ref.: #121209]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68368]	cytochrome oxidase	-	1.9.3.1	* from API 20E
[Ref.: #121209]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121209]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121209]	gelatinase	+
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #121209]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121209]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121209]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121209]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #121209]	oxidase	-
[Ref.: #121209]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121209]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #121209]	tween esterase	+
[Ref.: #121209]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #61746]	5869	-	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #61746]	3297	-	-	-	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+	-	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #121209]	10229	not determinedn.d.	+	-	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	+/-	+	+	+	+/-	+	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121209]	1364	not determinedn.d.	+	+	-	+	-	-	-	+	-	+	+	-	-	-	-	+	-	+	+	+	-	-	+	-	-	-	-	-	-	-	-	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	+	+	-	-	-	-	+	-	+	+	+	+	-	+	+	-	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121209]	8984	-	+	+	+	-	+	+	-	-	+	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4502]	Sample type/isolated from	pasteurized milk
[Ref.: #4502]	Geographic location (country and/or sea, region)	Bawaria
[Ref.: #4502]	Country	Germany
[Ref.: #4502]	Country ISO 3 Code	DEU
[Ref.: #4502]	Continent	Europe

[Ref.: #61746]	Sample type/isolated from	Pasteurized milk
[Ref.: #61746]	Sampling date	1992
[Ref.: #61746]	Country	Germany
[Ref.: #61746]	Country ISO 3 Code	DEU
[Ref.: #61746]	Continent	Europe

[Ref.: #121209]	Sample type/isolated from	Food, Pasteurized milk
[Ref.: #121209]	Country	Germany
[Ref.: #121209]	Country ISO 3 Code	DEU
[Ref.: #121209]	Continent	Europe
[Ref.: #121209]	Isolation date	1992
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Dairy product
#Engineered	#Treatment	#Preserved
#Engineered	#Treatment	#Sterilized (Desinfected)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM747230 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	446
Soil Samples	1920
Animal Samples	1524
Plant Samples	1079
Total Samples	4969

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4502]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121209]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4502]	Bacillus weihenstephanensis gene for 16S ribosomal RNA	AB021199	1531	ENA	1405 tax ID
[Ref.: #20218]	Bacillus weihenstephanensis partial 16S rRNA gene and ITS1, strain DSM 11821	AJ841876	543	ENA	86662 tax ID	*
[Ref.: #20218]	Bacillus weihenstephanensis clone DSMZ1182R 16S ribosomal RNA gene, partial sequence	DQ290004	1418	ENA	86662 tax ID	*
[Ref.: #20218]	Bacillus weihenstephanensis gene for 16S rRNA, partial sequence, strain: NBRC 101238	AB681419	1477	ENA	86662 tax ID	*
[Ref.: #20218]	Bacillus weihenstephanensis partial 16S rRNA gene and ITS1, strain WSBC 10204	AM747230	1663	ENA	86662 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus mycoides NBRC 101238 = DSM 11821	GCA_000513155	contig	GenBank	1220585 tax ID	*
[Ref.: #66792]	Bacillus weihenstephanensis NBRC 101238 = DSM 11821	1220585.4	wgs	PATRIC	1220585 tax ID	*
[Ref.: #66792]	Bacillus weihenstephanensis strain WSBC 10204	86662.17	complete	PATRIC	1405 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus weihenstephanensis strain WSBC 10204 PATRIC: 86662.17
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus weihenstephanensis strain WSBC 10204 PATRIC: 86662.17
[Ref.: #69480]	motile	yes	61.833	no
[Ref.: #69480]	flagellated	yes	77.345	no
[Ref.: #69480]	gram-positive	yes	89.371	yes
[Ref.: #69480]	anaerobic	no	98.592	yes
[Ref.: #69480]	halophile	no	94.183	no
[Ref.: #69480]	spore-forming	yes	93.43	yes
[Ref.: #69480]	glucose-util	yes	90.209	no
[Ref.: #69480]	aerobic	yes	76.164	no
[Ref.: #69480]	thermophile	no	99.328	no
[Ref.: #69480]	glucose-ferment	no	90.917	yes

Availability in culture collections External links

[Ref.: #4502]

Culture collection no.

DSM 11821, CCM 4872, CCUG 58725, LMG 18989, CIP 105772, KCTC 3975, WSBC 10204, WDCM 00222, WS 2480

[Ref.: #70824]

SI-ID 14497

Literature:

Topic	Title	Authors	Journal	DOI	Year
Stress	Bacillus weihenstephanensis can readily evolve for increased endospore heat resistance without compromising its thermotype.	Kim TD, Begyn K, Khanal S, Taghlaoui F, Heyndrickx M, Rajkovic A, Devlieghere F, Michiels C, Aertsen A	Int J Food Microbiol	10.1016/j.ijfoodmicro.2021.109072	2021	*
Metabolism	Temperature Dependence of the Proteome Profile of the Psychrotolerant Pathogenic Food Spoiler Bacillus weihenstephanensis Type Strain WSBC 10204.	Stelder SK, Mahmud SA, Dekker HL, de Koning LJ, Brul S, de Koster CG	J Proteome Res	10.1021/pr501307t	2015	*
Phylogeny	Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group.	Lechner S, Mayr R, Francis KP, Pruss BM, Kaplan T, Wiessner-Gunkel E, Stewart GS, Scherer S	Int J Syst Bacteriol	10.1099/00207713-48-4-1373	1998	*

References

#4502

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11821

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23304

SABINE LECHNER, RALF MAYR, KEVIN P. FRANCIS, BIRGIT M. PRüß, THOMAS KAPLAN, ELKE WIEßNER-GUNKEL, GORDON S. A. B. STEWART, SIEGFRIED SCHERER: Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. IJSEM 48: 1373 - 1382 1998 ( DOI 10.1099/00207713-48-4-1373 , PubMed 9828439 )

#38580 ; Curators of the CIP;
#61746 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 58725

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68368

Automatically annotated from API 20E .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70824

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID14497.1 )

#121209 Collection of Institut Pasteur ; Curators of the CIP; CIP 105772
* These data were automatically processed and therefore are not curated

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Bacillus weihenstephanensis strain WSBC 10204

Bacillus weihenstephanensis strain WSBC 10204

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