Name: Bacillus mycoides 273
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 745

Type strain:

Species: Bacillus mycoides

Strain Designation: 273, NRS-273

Culture col. no.: DSM 2048, ATCC 6462, CCUG 26678, NRRL B-14799, NCTC 12974, LMG 7128, CIP 103472, NRRL B-14811

Strain history: CIP <- 1991, DSMZ <- ATCC <- N.R. Smith: strain NRS-273

NCBI tax ID(s): 526997 (strain), 1405 (species)

SI-ID 1157

Special Collections

Other strains from Bacillus mycoides

Section

When using BacDive for research please cite our paper

Bacillus mycoides 273 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

Gram-positive
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus mycoides**
	Full Scientific Name (LPSN)	Bacillus mycoides Flügge 1886 (Approved Lists 1980)

Synonym

Bacillus weihenstephanensis

Information on morphological and physiological properties Morphology

[Ref.: #121210]	Gram stain	positive

[Ref.: #121210]	Cell shape	rod-shaped

[Ref.: #121210]	Motility	no

Multimedia content

[Ref.: #689]

Caption:

DSM 2048 Bacillus mycoides on Columbia Blood Agar

[Ref.: #689]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #689]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #689]

Culture medium growth

[Ref.: #689]

Culture medium link

Medium recipe at MediaDive

[Ref.: #689]

Culture medium composition

expand / minimize

[Ref.: #121210]

Culture medium

CIP Medium 3

[Ref.: #121210]

Culture medium growth

[Ref.: #121210]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121210]

Culture medium

CIP Medium 72

[Ref.: #121210]

Culture medium growth

[Ref.: #121210]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #689]			growth	25 °C
[Ref.: #48546]			growth	30-37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #48546]	Oxygen tolerance	aerobe
[Ref.: #125439]	Oxygen tolerance	facultative anaerobe Confidence in %96.7

[Ref.: #121210]	Ability of spore formation	yes
[Ref.: #125438]	Ability of spore formation	yes Confidence in %90.341
[Ref.: #125439]	Ability of spore formation	yes Confidence in %95.1

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121210]	425	not determinedn.d.	+	+	+	+	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	-	+	+	-	+	+	-	+	-	+	+	+	+	-	+	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #689]	Sample type/isolated from	soil

[Ref.: #48546]	Sample type/isolated from	Soil

[Ref.: #121210]	Sample type/isolated from	Environment, Soil
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB021192 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	446
Soil Samples	1920
Animal Samples	1524
Plant Samples	1079
Total Samples	4969

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #689]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121210]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus mycoides gene for 16S ribosomal RNA	AB021192	1513	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	Bacillus mycoides strain 6462 16S ribosomal RNA gene, partial sequence	AF155956	1508	GenBank ENA	1405 tax ID	*
[Ref.: #124043]	Bacillus mycoides partial 16S rRNA gene and ITS1, strain CIP 103472	AM747229	1663	GenBank ENA	1405 tax ID	*
[Ref.: #124043]	Bacillus mycoides strain DSM 2048 16S ribosomal RNA gene, partial sequence.	ON872258	555	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	Bacillus mycoides clone DSMZ2048R 16S ribosomal RNA gene, partial sequence	DQ290003	1411	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	B.mycoides 16S ribosomal RNA	X55061	1450	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	Bacillus mycoides strain ATCC6462 16S-23S ribosomal RNA intergenic spacer, partial sequence	AF267906	144	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	Bacillus mycoides 16S rRNA gene, strain DSM 2048T	Z84592	832	GenBank ENA	1396 tax ID	*
[Ref.: #20218]	Bacillus mycoides partial 16S rRNA gene and ITS1, strain DSM 2048	AJ841874	543	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	Bacillus mycoides gene for 16S rRNA, partial sequence	AB006931	277	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	Bacillus mycoides 16S-23S rRNA intergenic spacer, DNA sequence	L37577	144	GenBank ENA	1405 tax ID	*
[Ref.: #20218]	Bacillus mycoides gene for 16S rRNA, partial sequence, strain: GTC 01994	AB592538	1430	GenBank ENA	1405 tax ID	*
[Ref.: #124043]	Bacillus mycoides DSM 2048 partial 16S rRNA gene, strain JC1004	LS998020	892	GenBank ENA	526997 tax ID	*
[Ref.: #20218]	Bacillus mycoides strain ATCC 6462 16S ribosomal RNA gene, partial sequence	EF210295	1461	GenBank ENA	1405 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus mycoides DSM 2048	GCA_022630575	complete	GenBank ENA	1405 tax ID	*
[Ref.: #66792]	Bacillus mycoides DSM 2048	GCA_000003925	chromosome	GenBank ENA	526997 tax ID	*
[Ref.: #66792]	Bacillus mycoides ATCC 6462	1405.10	complete	PATRIC	1405 tax ID	*
[Ref.: #66792]	Bacillus mycoides DSM 2048	526997.3	wgs	PATRIC	526997 tax ID	*
[Ref.: #66792]	Bacillus mycoides strain ATCC 6462	1405.80	plasmid	PATRIC	1405 tax ID	*
[Ref.: #66792]	Bacillus mycoides strain ATCC 6462	1405.82	plasmid	PATRIC	1405 tax ID	*

[Ref.: #689]	GC-content	34.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #689]	GC-content	34.1 mol%	Buoyant density centrifugation (BD)

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bacillus mycoides DSM 2048 DSM 2048 NCBI: GCA_000003925.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	yes	77.373	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	93.858	no
[Ref.: #125438]	aerobic	aerobicⓘ	yes	63.065	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	yes	90.341	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	94.847	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	yes	84.431	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Bacillus mycoides ATCC 6462 NCBI: GCA_000832605
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	yes	95.1
[Ref.: #125439]	BacteriaNet	motilityⓘ	yes	89.7
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	positive	69.7
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	facultative anaerobe	96.7

Availability in culture collections External links

[Ref.: #689]

Culture collection no.

DSM 2048, ATCC 6462, CCUG 26678, NRRL B-14799, NCTC 12974, LMG 7128, CIP 103472, NRRL B-14811

[Ref.: #70419]

SI-ID 1157

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice.	Yamada S, Ohashi E, Agata N, Venkateswaran K	Appl Environ Microbiol	10.1128/AEM.65.4.1483-1490.1999	1999	*

References

#689	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2048

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#48546 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 26678

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#70419

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1157.1 )

#121210 Collection of Institut Pasteur ; Curators of the CIP; CIP 103472

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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Bacillus mycoides DSM 2048 DSM 2048

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