Strain identifier

BacDive ID: 745

Type strain: Yes

Species: Bacillus mycoides

Strain Designation: 273, NRS-273

Strain history: CIP <- 1991, DSMZ <- ATCC <- N.R. Smith: strain NRS-273

NCBI tax ID(s): 526997 (strain), 1405 (species)

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General

@ref: 689

BacDive-ID: 745

DSM-Number: 2048

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, motile

description: Bacillus mycoides 273 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
526997strain
1405species

strain history

@refhistory
689<- ATCC <- N.R. Smith, 273
121210CIP <- 1991, DSMZ <- ATCC <- N.R. Smith: strain NRS-273

doi: 10.13145/bacdive745.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus mycoides
  • full scientific name: Bacillus mycoides Flügge 1886 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus weihenstephanensis

@ref: 689

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus mycoides

full scientific name: Bacillus mycoides Flügge 1886 emend. Liu et al. 2018

strain designation: 273, NRS-273

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes92.146
69480100positive
121210nopositiverod-shaped

multimedia

  • @ref: 689
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_2048.jpg
  • caption: DSM 2048 Bacillus mycoides on Columbia Blood Agar
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
689NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
121210CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
121210CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
689positivegrowth25mesophilic
48546positivegrowth30-37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 48546
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481yes100
69480yes100
121210yes

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121210++++--++++++++-+++++-----------+-----------------++--+-----++---------++++++----++-++-+-++++-++++++

Isolation, sampling and environmental information

isolation

@refsample type
689soil
48546Soil
121210Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_26.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_21;97_22;98_25;99_26&stattab=map
  • Last taxonomy: Bacillus
  • 16S sequence: AB021192
  • Sequence Identity:
  • Total samples: 4969
  • soil counts: 1920
  • aquatic counts: 446
  • animal counts: 1524
  • plant counts: 1079

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
6892Risk group (German classification)
1212101Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bacillus mycoides gene for 16S rRNA, partial sequenceAB006931277ena1405
20218Bacillus mycoides gene for 16S ribosomal RNAAB0211921513ena1405
20218Bacillus mycoides gene for 16S rRNA, partial sequence, strain: GTC 01994AB5925381430ena1405
20218Bacillus mycoides strain 6462 16S ribosomal RNA gene, partial sequenceAF1559561508ena1405
20218Bacillus mycoides strain ATCC6462 16S-23S ribosomal RNA intergenic spacer, partial sequenceAF267906144ena1405
20218Bacillus mycoides partial 16S rRNA gene and ITS1, strain DSM 2048AJ841874543ena1405
20218Bacillus mycoides clone DSMZ2048R 16S ribosomal RNA gene, partial sequenceDQ2900031411ena1405
20218Bacillus mycoides strain ATCC 6462 16S ribosomal RNA gene, partial sequenceEF2102951461ena1405
20218Bacillus mycoides 16S-23S rRNA intergenic spacer, DNA sequenceL37577144ena1405
20218B.mycoides 16S ribosomal RNAX550611450ena1405
20218Bacillus mycoides 16S rRNA gene, strain DSM 2048TZ84592832ena1396

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus mycoides ATCC 6462GCA_000832605completencbi1405
66792Bacillus mycoides DSM 2048GCA_000003925chromosomencbi526997
66792Bacillus mycoides ATCC 64621405.10completepatric1405
66792Bacillus mycoides DSM 2048526997.3wgspatric526997
66792Bacillus mycoides strain ATCC 64621405.80plasmidpatric1405
66792Bacillus mycoides strain ATCC 64621405.82plasmidpatric1405
66792Bacillus mycoides strain ATCC 64621405.81plasmidpatric1405
66792Bacillus mycoides ATCC 64622623621012completeimg1405
66792Bacillus mycoides DSM 2048643886175draftimg526997
66792Bacillus mycoides DSM 2048GCA_022630575completencbi1405

GC content

@refGC-contentmethod
68934.2thermal denaturation, midpoint method (Tm)
68934.1Buoyant density centrifugation (BD)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno65.411no
flagellatedyes61.819no
gram-positiveyes90.913no
anaerobicno98.941no
halophileno91.178no
spore-formingyes91.719no
thermophileno99.69no
glucose-utilyes88.959no
aerobicyes87.542yes
glucose-fermentno89.966no

External links

@ref: 689

culture collection no.: DSM 2048, ATCC 6462, CCUG 26678, NRRL B-14799, NCTC 12974, LMG 7128, CIP 103472, NRRL B-14811

straininfo link

  • @ref: 70419
  • straininfo: 1157

literature

  • topic: Phylogeny
  • Pubmed-ID: 10103241
  • title: Cloning and nucleotide sequence analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and their application to the detection of B. cereus in rice.
  • authors: Yamada S, Ohashi E, Agata N, Venkateswaran K
  • journal: Appl Environ Microbiol
  • DOI: 10.1128/AEM.65.4.1483-1490.1999
  • year: 1999
  • mesh: Amino Acid Sequence, Bacillus/*classification/*genetics, Bacillus cereus/genetics/*isolation & purification, Bacterial Typing Techniques, Base Sequence, Cloning, Molecular, DNA Gyrase, DNA Topoisomerases, Type II/*genetics, DNA, Ribosomal, Molecular Sequence Data, Nucleic Acid Hybridization, Oryza/*microbiology, Polymerase Chain Reaction/methods, RNA, Ribosomal, 16S/genetics, Sequence Alignment, Sequence Analysis, DNA
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
689Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2048)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2048
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
48546Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 26678)https://www.ccug.se/strain?id=26678
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70419Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1157.1StrainInfo: A central database for resolving microbial strain identifiers
121210Curators of the CIPCollection of Institut Pasteur (CIP 103472)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103472