Strain identifier

BacDive ID: 1154

Type strain: Yes

Species: Bacillus horti

Strain Designation: K 13, K13

Strain history: CIP <- 1998, JCM <- I. Yumoto: strain K13

NCBI tax ID(s): 77523 (species)

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General

@ref: 4802

BacDive-ID: 1154

DSM-Number: 12751

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-negative, motile, rod-shaped

description: Bacillus horti K 13 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from garden soil.

NCBI tax id

  • NCBI tax id: 77523
  • Matching level: species

strain history

@refhistory
4802<- I. Yumoto, HNIRI, Japan; K 13
67770I. Yumoto K13.
119974CIP <- 1998, JCM <- I. Yumoto: strain K13

doi: 10.13145/bacdive1154.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus horti
  • full scientific name: Bacillus horti Yumoto et al. 1998

@ref: 4802

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus horti

full scientific name: Bacillus horti Yumoto et al. 1998

strain designation: K 13, K13

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
23305negative1.5-6.0 µm0.6-0.8 µmrod-shapedyesperitrichous
119974negativerod-shapedyes

colony morphology

@refcolony color
23305white
119974

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4802ALKALINE NUTRIENT AGAR (DSMZ Medium 31)yeshttps://mediadive.dsmz.de/medium/31Name: ALKALINE NUTRIENT AGAR (DSMZ Medium 31) Composition: Agar 15.0 g/l Na2CO3 anhydrous 5.3 g/l Peptone 5.0 g/l NaHCO3 4.2 g/l Meat extract 3.0 g/l Distilled water
35192MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119974CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
119974CIP Medium 291yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=291

culture temp

@refgrowthtypetemperaturerange
4802positivegrowth30mesophilic
23305nogrowth10psychrophilic
23305positivegrowth40thermophilic
23305nogrowth50thermophilic
23305nogrowth5psychrophilic
23305positivegrowth15psychrophilic
23305nogrowth45thermophilic
23305nogrowth55thermophilic
35192positivegrowth30mesophilic
67770positivegrowth30mesophilic
119974positivegrowth30-37mesophilic
119974nogrowth10psychrophilic
119974nogrowth45thermophilic
119974nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
23305positivegrowth7
23305positiveoptimum8-10alkaliphile
119974nogrowth6

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23305obligate aerobe
119974obligate aerobe

spore formation

  • @ref: 23305
  • spore description: subterminally located ellipsoidal spores
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
23305NaClpositivegrowth10 %
23305NaClpositivegrowth5 %
23305NaClpositivegrowth3 %
23305NaClpositivegrowth7 %
23305NaClpositivegrowth9 %
23305NaClpositivegrowth11 %
23305NaClnogrowth12 %
23305NaClnogrowth13 %
23305NaClnogrowth14 %
23305NaClnogrowth15 %
23305NaClnogrowth16 %
23305NaClnogrowth17 %
23305NaClnogrowth18 %
23305NaClnogrowth20 %
119974NaClpositivegrowth6-10 %
119974NaClnogrowth0 %
119974NaClnogrowth2 %
119974NaClnogrowth4 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2330528044phenylalanine-assimilation
2330522599arabinose-builds acid from
2330512936D-galactose-builds acid from
2330517113erythritol-builds acid from
2330562345L-rhamnose-builds acid from
2330517268myo-inositol-builds acid from
2330517992sucrose-builds acid from
2330515824D-fructose-builds gas from
2330517634D-glucose-builds gas from
2330516988D-ribose-builds gas from
2330565327D-xylose-builds gas from
2330553424tween 20-hydrolysis
2330553423tween 40-hydrolysis
2330553425tween 60-hydrolysis
2330553426tween 80-hydrolysis
2330516199urea-hydrolysis
2330515824D-fructose+builds acid from
2330517634D-glucose+builds acid from
2330516988D-ribose+builds acid from
2330565327D-xylose+builds acid from
23305casein+hydrolysis
2330516991dna+hydrolysis
233055291gelatin+hydrolysis
23305606565hippurate+hydrolysis
2330528017starch+hydrolysis
2330517632nitrate+reduction
11997416947citrate-carbon source
1199744853esculin-hydrolysis
119974606565hippurate+hydrolysis
11997417632nitrate+reduction
11997416301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2330535581indoleno
11997435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11997415688acetoin-
11997417234glucose-

enzymes

@refvalueactivityec
23305beta-galactosidase+3.2.1.23
23305catalase+1.11.1.6
23305cytochrome oxidase+1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119974oxidase+
119974beta-galactosidase+3.2.1.23
119974alcohol dehydrogenase-1.1.1.1
119974gelatinase+
119974amylase+
119974DNase+
119974caseinase+3.4.21.50
119974catalase+1.11.1.6
119974tween esterase-
119974gamma-glutamyltransferase-2.3.2.2
119974lecithinase-
119974lipase-
119974lysine decarboxylase-4.1.1.18
119974ornithine decarboxylase-4.1.1.17
119974protease-
119974tryptophan deaminase-
119974urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119974-+++-+---+++----+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119974----------+----------+-----------+------------------------------------------------+---------------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
4802garden soilHokkaido, AstumaJapanJPNAsia
67770SoilAtsuma, HokkaidoJapanJPNAsia
119974Environment, SoilJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Engineered#Agriculture#Garden

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
48021Risk group (German classification)
1199741Risk group (French classification)

Sequence information

16S sequences

  • @ref: 23305
  • description: Bacillus horti gene for 16S rRNA, partial sequence
  • accession: D87035
  • length: 1512
  • database: nuccore
  • NCBI tax ID: 77523

Genome sequences

  • @ref: 66792
  • description: Bacillus horti DSM 12751
  • accession: 2926514369
  • assembly level: draft
  • database: img
  • NCBI tax ID: 77523

GC content

@refGC-contentmethod
2330540.9high performance liquid chromatography (HPLC)
6777040high performance liquid chromatography (HPLC)

External links

@ref: 4802

culture collection no.: DSM 12751, JCM 9943, ATCC 700778, CGMCC 1.3650, CIP 105746, KCTC 3838, LMG 18497

straininfo link

  • @ref: 70825
  • straininfo: 12121

literature

  • topic: Phylogeny
  • Pubmed-ID: 9731298
  • title: Bacillus horti sp. nov., a new gram-negative alkaliphilic bacillus.
  • authors: Yumoto I, Yamazaki K, Sawabe T, Nakano K, Kawasaki K, Ezura Y, Shinano H
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-48-2-565
  • year: 1998
  • mesh: Bacillus/*classification/physiology/ultrastructure, Base Sequence, DNA, Bacterial, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/analysis, RNA, Ribosomal, 16S/analysis, Sequence Analysis, RNA
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4802Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12751)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12751
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23305Isao Yumoto, Koji Yamazaki, Tomoo Sawabe, Kazuaki Nakano, Kosei Kawasaki, Yoshio Ezura, Haruo Shinano10.1099/00207713-48-2-565Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillusIJSEM 48: 565-571 19989731298
35192Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17799
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
70825Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12121.1StrainInfo: A central database for resolving microbial strain identifiers
119974Curators of the CIPCollection of Institut Pasteur (CIP 105746)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105746