Name: Nocardia blacklockiae N444
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 10822

Type strain:

Species: Nocardia blacklockiae

Strain Designation: N444

Culture col. no.: DSM 45135, ATCC 700035, JCM 16005

Strain history: DSM 45135 <-- P. S. Conville <-- R. J. Wallace N444 <-- Louisiana State Univ. Med. Sch., USA.

NCBI tax ID(s): 480036 (species)

SI-ID 43740

ATCC 700035, DSM 45135, JCM 16005

Special Collections

Section

Nocardia blacklockiae N444 is a bacterium that builds an aerial mycelium and was isolated from human muscle abscess.

16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Nocardiaceae*
	Genus	*Nocardia*
	Species	**Nocardia blacklockiae**
	Full Scientific Name (LPSN)	Nocardia blacklockiae Conville et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #69373]	Colony color	Dahlia yellow (1033)
[Ref.: #69373]	Cultivation medium used	ISP 7

[Ref.: #69373]	Colony color	Daffodil yellow (1007)
[Ref.: #69373]	Cultivation medium used	ISP 2

[Ref.: #69373]	Colony color	Daffodil yellow (1007)
[Ref.: #69373]	Cultivation medium used	ISP 4

[Ref.: #69373]	Colony color	Daffodil yellow (1007)
[Ref.: #69373]	Cultivation medium used	ISP 6

[Ref.: #69373]	Colony color	Daffodil yellow ( 1007)
[Ref.: #69373]	Cultivation medium used	ISP 3

[Ref.: #69373]	Colony color	Maize yellow (1006)
[Ref.: #69373]	Cultivation medium used	suter with tyrosine

[Ref.: #69373]	Colony color	Maize yellow (1006)
[Ref.: #69373]	Cultivation medium used	suter without tyrosine

[Ref.: #69373]	Colony color	Pastel orange (2003)
[Ref.: #69373]	Cultivation medium used	ISP 5

[Ref.: #69373]	Pigment production	no
[Ref.: #69373]	Pigment name	Melanin
[Ref.: #69373]	Pigment production	no
[Ref.: #69373]	Pigment name	soluble pigment

[Ref.: #69373]	Multicellular complex forming ability	yes
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Multicellular complex color	Cream (9001)
[Ref.: #69373]	Medium Name (multicellularity)	ISP 2

[Ref.: #69373]	Multicellular complex forming ability	no
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Medium Name (multicellularity)	ISP 3

[Ref.: #69373]	Multicellular complex forming ability	no
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Medium Name (multicellularity)	ISP 4

[Ref.: #69373]	Multicellular complex forming ability	no
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Medium Name (multicellularity)	ISP 5

[Ref.: #69373]	Multicellular complex forming ability	yes
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Multicellular complex color	Cream (9001)
[Ref.: #69373]	Medium Name (multicellularity)	ISP 6

[Ref.: #69373]	Multicellular complex forming ability	yes
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Multicellular complex color	Light ivory (1015)
[Ref.: #69373]	Medium Name (multicellularity)	ISP 7

[Ref.: #69373]	Multicellular complex forming ability	yes
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Multicellular complex color	Beige (1001)
[Ref.: #69373]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69373]	Multicellular complex forming ability	yes
[Ref.: #69373]	Multicellular complex name	Aerial mycelium
[Ref.: #69373]	Multicellular complex color	Cream (9001)
[Ref.: #69373]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69373]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69373]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69373]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69373]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12382]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #12382]

Culture medium growth

[Ref.: #12382]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12382]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #12382]			growth	28 °C
[Ref.: #67770]			growth	28 °C

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69373]		NaCl		growth	0-5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69373]	arabinose ChEBI	-	growth
[Ref.: #69373]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69373]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69373]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69373]	inositol ChEBI	-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69373]	mannose ChEBI	+/-	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69373]	raffinose ChEBI	-	growth
[Ref.: #69373]	rhamnose ChEBI	-	growth
[Ref.: #69373]	sucrose ChEBI	-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69373]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69373]	3866	-	-	-	+	-	-	+/-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69373]	6050	not determinedn.d.	+	+/-	-	-	+/-	-	-	-	-	+	+	-	+/-	-	+	+/-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12382]	Sample type/isolated from	human muscle abscess
[Ref.: #12382]	Country	USA
[Ref.: #12382]	Country ISO 3 Code	USA
[Ref.: #12382]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Human muscle abscess
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Other	#Abscess

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12382]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12382]	Nocardia blacklockiae strain ATCC 700035 16S ribosomal RNA gene, partial sequence	EU099360	1414	GenBank ENA	480036 tax ID
[Ref.: #20218]	Nocardia blacklockiae 16S ribosomal RNA gene, partial sequence	GQ376162	1441	GenBank ENA	480036 tax ID	*

Availability in culture collections External links

[Ref.: #12382]

Culture collection no.

DSM 45135, ATCC 700035, JCM 16005

[Ref.: #80083]

SI-ID 43740

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the "Nocardia transvalensis Complex".	Conville PS, Brown JM, Steigerwalt AG, Brown-Elliott BA, Witebsky FG	J Clin Microbiol	10.1128/JCM.02011-07	2008	*

References

#12382

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45135

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69373

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#80083

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43740.1 )

* These data were automatically processed and therefore are not curated

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