Strain identifier

BacDive ID: 10822

Type strain: Yes

Species: Nocardia blacklockiae

Strain Designation: N444

Strain history: DSM 45135 <-- P. S. Conville <-- R. J. Wallace N444 <-- Louisiana State Univ. Med. Sch., USA.

NCBI tax ID(s): 480036 (species)

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General

@ref: 12382

BacDive-ID: 10822

DSM-Number: 45135

keywords: 16S sequence, Bacteria, mesophilic

description: Nocardia blacklockiae N444 is a mesophilic bacterium that builds an aerial mycelium and was isolated from human muscle abscess.

NCBI tax id

  • NCBI tax id: 480036
  • Matching level: species

strain history

@refhistory
12382<- P. S. Conville; ATCC 700035 <- R. J. Wallace <- Louisiana State Univ. Med. School
67770DSM 45135 <-- P. S. Conville <-- R. J. Wallace N444 <-- Louisiana State Univ. Med. Sch., USA.

doi: 10.13145/bacdive10822.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Nocardia
  • species: Nocardia blacklockiae
  • full scientific name: Nocardia blacklockiae Conville et al. 2009

@ref: 12382

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Nocardia

species: Nocardia blacklockiae

full scientific name: Nocardia blacklockiae Conville et al. 2009

strain designation: N444

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69373Dahlia yellow (1033)ISP 7
69373Daffodil yellow (1007)ISP 2
69373Daffodil yellow (1007)ISP 4
69373Daffodil yellow (1007)ISP 6
69373Daffodil yellow ( 1007)ISP 3
69373Maize yellow (1006)suter with tyrosine
69373Maize yellow (1006)suter without tyrosine
69373Pastel orange (2003)ISP 5

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69373yesAerial myceliumCream (9001)ISP 2
69373noAerial myceliumISP 3
69373noAerial myceliumISP 4
69373noAerial myceliumISP 5
69373yesAerial myceliumCream (9001)ISP 6
69373yesAerial myceliumLight ivory (1015)ISP 7
69373yesAerial myceliumBeige (1001)suter with tyrosine
69373yesAerial myceliumCream (9001)suter without tyrosine

pigmentation

@refproductionname
69373noMelanin
69373nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
69373DSM_45135_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69373DSM_45135_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

  • @ref: 12382
  • name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/65
  • composition: Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12382positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

halophily

  • @ref: 69373
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6937322599arabinose-growth
6937362968cellulose-growth
6937328757fructose+growth
6937317234glucose+growth
6937317268inositol-growth
6937337684mannose+/-growth
6937316634raffinose-growth
6937326546rhamnose-growth
6937317992sucrose-growth
6937318222xylose-growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69373---+--+/-------------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69373++/---+/-----++-+/--++/----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
12382human muscle abscessUSAUSANorth America
67770Human muscle abscessUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Other#Abscess

Safety information

risk assessment

  • @ref: 12382
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Nocardia blacklockiae 16S ribosomal RNA gene, partial sequenceGQ3761621441ena480036
12382Nocardia blacklockiae strain ATCC 700035 16S ribosomal RNA gene, partial sequenceEU0993601414ena480036

External links

@ref: 12382

culture collection no.: DSM 45135, ATCC 700035, JCM 16005

straininfo link

  • @ref: 80083
  • straininfo: 43740

literature

  • topic: Phylogeny
  • Pubmed-ID: 18256227
  • title: Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the "Nocardia transvalensis Complex".
  • authors: Conville PS, Brown JM, Steigerwalt AG, Brown-Elliott BA, Witebsky FG
  • journal: J Clin Microbiol
  • DOI: 10.1128/JCM.02011-07
  • year: 2008
  • mesh: Adult, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Heat-Shock Proteins/genetics, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Sequence Data, Nocardia/*classification/drug effects/genetics/physiology, Nocardia Infections/*microbiology, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12382Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45135)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45135
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69373Wink, J.https://cdn.dsmz.de/wink/DSM%2045135.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
80083Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID43740.1StrainInfo: A central database for resolving microbial strain identifiers