Nocardia wallacei (DSM 45136, ATCC 49873, JCM 16006)

Name: Nocardia wallacei (DSM 45136, ATCC 49873, JCM 16006)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10821

Type strain

Strain Designation N 44, TDHR 168830

Culture col. no. DSM 45136 ATCC 49873 JCM 16006 N 44 TDHR 168830

NCBI tax ID(s) 480035

Special Collections DSMZ JCM Wink compendium

History <- P. S. Conville; ATCC 49873 <- R. J. Wallace <- Texas Dept. of Health, TDHR 168330 DSM 45136 <-- P. S. Conville <-- R. J. Wallace N44 <-- Texas Dept. Health, USA, TDHR 168330.

Links

Nocardia wallacei N 44 is a bacterium that builds an aerial mycelium and was isolated from sputum.

16S sequence Bacteria

Name and taxonomic classification

SI-ID 42728

ATCC 49873,DSM 45136,JCM 16006

@ref 20215

Last LPSN update 2026-02-09 11:20:21

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Nocardiaceae

Genus Nocardia

Species Nocardia wallacei

Full scientific name Nocardia wallacei Conville et al. 2009

BacDive ID	Other strains from **Nocardia wallacei** (2)	Type strain
23156	N. wallacei AB 137, DSM 45846
152116	N. wallacei CCUG 45829

Morphology

Colony morphology

@ref	Colony color	Medium used
69374	Brown beige (3012)	suter with tyrosine
69374	Brown beige (1011)	suter without tyrosine
69374	Beige red (3012)	ISP 7
69374	Beige red (3012)	ISP 5
69374	Pastel yellow (1034)	ISP 3
69374	Pastel yellow (1034)	ISP 4
69374	Pastel yellow (1034)	ISP 6
69374	Yellow orange (2000)	ISP 2

Multicellular morphology

@ref	Complex name	Complex color	Medium name
69374	Aerial mycelium	Cream (9001)	ISP 2
69374	Aerial mycelium	Cream (9001)	ISP 3
69374	Aerial mycelium		ISP 4
69374	Aerial mycelium	Light ivory 81015)	ISP 5
69374	Aerial mycelium		ISP 6
69374	Aerial mycelium	Light ivory (1015)	ISP 7
69374	Aerial mycelium	Light ivory (1015)	suter with tyrosine
69374	Aerial mycelium	Light ivory (1015)	suter without tyrosine

Pigmentation

@ref	Production	Name
69374		melanin pigment
69374		soluble pigment

Multimedia

@ref:	12381
multimedia content:	DSM_45136.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45136.jpg
caption:	Medium 535 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	69374
multimedia content:	DSM_45136_image3.jpeg
multimedia.multimedia content:	DSM_45136_image3.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

@ref:	69374
multimedia content:	DSM_45136_image4.jpeg
multimedia.multimedia content:	DSM_45136_image4.jpeg
caption:	Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)
intellectual property rights:	Helmholtz-Zentrum für Infektionsforschung GmbH
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
12381	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)		Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
12381	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
12381	positive	growth	28
67770	positive	growth	28

Physiology and metabolism

Halophily

@ref	Salt	Growth	Tested relation	Concentration
69374	NaCl	positive	growth	0-5 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
69374	22599 ChEBI	arabinose	-	growth
69374	62968 ChEBI	cellulose	-	growth
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
69374	28757 ChEBI	fructose	-	growth
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
69374	17234 ChEBI	glucose	+	growth
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
69374	17268 ChEBI	inositol	-	growth
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
69374	37684 ChEBI	mannose	-	growth
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
69374	16634 ChEBI	raffinose	-	growth
69374	26546 ChEBI	rhamnose	-	growth
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
69374	17992 ChEBI	sucrose	-	growth
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne
69374	18222 ChEBI	xylose	-	growth

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68379	urease	+	3.5.1.5	from API Coryne

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
69374	+	-	-	+	-	-	+	-	+	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
69374	not determinedn.d.	+	+	+	+/-	+/-	+/-	+/-	-	-	+	+/-	-	+/-	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12381	sputum	Texas	USA	USA	North America
67770	Sputum	TX	USA	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
12381	2	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Nocardia wallacei strain W8083 16S ribosomal RNA gene, partial sequence	GQ853074	1441	480035
20218	Nocardia wallacei strain DSM 45136 16S ribosomal RNA gene, partial sequence	JN041527	462	480035
20218	N.asteroides ATCC 49873 DNA for 16S rRNA	Z82226	1318	480035
12381	Nocardia wallacei strain ATCC 49873 16S ribosomal RNA gene, partial sequence	EU099357	1388	480035

GC content

@ref	GC-content (mol%)	Method
12381	65.0
67770	65	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Nocardia wallacei: A rare cause of actinomycetoma in an immunocompetent patient.	Chieosilapatham P, Duangsonk K, Kaweewan I, Tongjai S, Kanthawang T.	Eur J Microbiol Immunol (Bp)	10.1556/1886.2024.00110	2024
Enzymology	Evaluation of the integrated database network system (IDNS) SmartGene software for analysis of 16S rRNA gene sequences for identification of Nocardia species.	Conville PS, Murray PR, Zelazny AM.	J Clin Microbiol	10.1128/jcm.00681-10	2010
Phylogeny	Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the "Nocardia transvalensis Complex".	Conville PS, Brown JM, Steigerwalt AG, Brown-Elliott BA, Witebsky FG	J Clin Microbiol	10.1128/JCM.02011-07	2008

References

#12381	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45136
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69374	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nocardia wallacei (DSM 45136, ATCC 49873, JCM 16006)

Name and taxonomic classification

Morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Halophily

Metabolite utilization

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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