Name: Nocardia wallacei N 44
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 10821

Type strain:

Species: Nocardia wallacei

Strain Designation: N 44, TDHR 168830

Culture col. no.: DSM 45136, ATCC 49873, JCM 16006

Strain history: DSM 45136 <-- P. S. Conville <-- R. J. Wallace N44 <-- Texas Dept. Health, USA, TDHR 168330.

NCBI tax ID(s): 480035 (species)

SI-ID 42728

ATCC 49873, DSM 45136, JCM 16006

Other strains from Nocardia wallacei

N. wallacei AB 137, DSM 45846
N. wallacei CCUG 45829

Section

Nocardia wallacei N 44 is a mesophilic bacterium that builds an aerial mycelium and was isolated from sputum.

mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Nocardiaceae*
	Genus	*Nocardia*
	Species	**Nocardia wallacei**
	Full Scientific Name (LPSN)	Nocardia wallacei Conville et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #69374]	Colony color	Brown beige (3012)
[Ref.: #69374]	Cultivation medium used	suter with tyrosine

[Ref.: #69374]	Colony color	Brown beige (1011)
[Ref.: #69374]	Cultivation medium used	suter without tyrosine

[Ref.: #69374]	Colony color	Beige red (3012)
[Ref.: #69374]	Cultivation medium used	ISP 7

[Ref.: #69374]	Colony color	Beige red (3012)
[Ref.: #69374]	Cultivation medium used	ISP 5

[Ref.: #69374]	Colony color	Pastel yellow (1034)
[Ref.: #69374]	Cultivation medium used	ISP 3

[Ref.: #69374]	Colony color	Pastel yellow (1034)
[Ref.: #69374]	Cultivation medium used	ISP 4

[Ref.: #69374]	Colony color	Pastel yellow (1034)
[Ref.: #69374]	Cultivation medium used	ISP 6

[Ref.: #69374]	Colony color	Yellow orange (2000)
[Ref.: #69374]	Cultivation medium used	ISP 2

[Ref.: #69374]	Pigment production	no
[Ref.: #69374]	Pigment name	Melanin
[Ref.: #69374]	Pigment production	no
[Ref.: #69374]	Pigment name	soluble pigment

[Ref.: #69374]	Multicellular complex forming ability	yes
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Multicellular complex color	Cream (9001)
[Ref.: #69374]	Medium Name (multicellularity)	ISP 2

[Ref.: #69374]	Multicellular complex forming ability	yes
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Multicellular complex color	Cream (9001)
[Ref.: #69374]	Medium Name (multicellularity)	ISP 3

[Ref.: #69374]	Multicellular complex forming ability	no
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Medium Name (multicellularity)	ISP 4

[Ref.: #69374]	Multicellular complex forming ability	yes
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Multicellular complex color	Light ivory 81015)
[Ref.: #69374]	Medium Name (multicellularity)	ISP 5

[Ref.: #69374]	Multicellular complex forming ability	no
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Medium Name (multicellularity)	ISP 6

[Ref.: #69374]	Multicellular complex forming ability	yes
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Multicellular complex color	Light ivory (1015)
[Ref.: #69374]	Medium Name (multicellularity)	ISP 7

[Ref.: #69374]	Multicellular complex forming ability	yes
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Multicellular complex color	Light ivory (1015)
[Ref.: #69374]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69374]	Multicellular complex forming ability	yes
[Ref.: #69374]	Multicellular complex name	Aerial mycelium
[Ref.: #69374]	Multicellular complex color	Light ivory (1015)
[Ref.: #69374]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #12381]

Caption:

Medium 535 28°C

[Ref.: #12381]

Intellectual property rights:

[Ref.: #69374]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69374]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69374]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69374]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #12381]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #12381]

Culture medium growth

[Ref.: #12381]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12381]

Culture medium composition

expand / minimize

[Ref.: #12381]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #12381]

Culture medium growth

[Ref.: #12381]

Culture medium link

Medium recipe at MediaDive

[Ref.: #12381]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #12381]			growth	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #12381]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69374]		NaCl		growth	0-5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69374]	arabinose ChEBI	-	growth
[Ref.: #69374]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69374]	fructose ChEBI	-	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69374]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69374]	inositol ChEBI	-	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69374]	mannose ChEBI	-	growth
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #69374]	raffinose ChEBI	-	growth
[Ref.: #69374]	rhamnose ChEBI	-	growth
[Ref.: #69374]	sucrose ChEBI	-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69374]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68379]	beta-glucosidase	+	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69374]	3867	+	-	-	+	-	-	+	-	+	+	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69374]	6051	not determinedn.d.	+	+	+	+/-	+/-	+/-	+/-	-	-	+	+/-	-	+/-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12381]	Sample type/isolated from	sputum
[Ref.: #12381]	Geographic location (country and/or sea, region)	Texas
[Ref.: #12381]	Country	USA
[Ref.: #12381]	Country ISO 3 Code	USA
[Ref.: #12381]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Sputum
[Ref.: #67770]	Geographic location (country and/or sea, region)	TX
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host Body Product

#Fluids

#Sputum

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12381]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12381]	Nocardia wallacei strain ATCC 49873 16S ribosomal RNA gene, partial sequence	EU099357	1388	ENA	480035 tax ID
[Ref.: #20218]	Nocardia wallacei strain W8083 16S ribosomal RNA gene, partial sequence	GQ853074	1441	ENA	480035 tax ID	*
[Ref.: #20218]	N.asteroides ATCC 49873 DNA for 16S rRNA	Z82226	1318	ENA	480035 tax ID	*
[Ref.: #20218]	Nocardia wallacei strain DSM 45136 16S ribosomal RNA gene, partial sequence	JN041527	462	ENA	480035 tax ID	*

[Ref.: #12381]	GC-content	65.0 mol%
[Ref.: #67770]	GC-content	65 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #12381]

Culture collection no.

DSM 45136, ATCC 49873, JCM 16006

[Ref.: #80082]

SI-ID 42728

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the "Nocardia transvalensis Complex".	Conville PS, Brown JM, Steigerwalt AG, Brown-Elliott BA, Witebsky FG	J Clin Microbiol	10.1128/JCM.02011-07	2008	*

References

#12381

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45136

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69374

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#80082

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42728.1 )

* These data were automatically processed and therefore are not curated

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