Strain identifier

BacDive ID: 10821

Type strain: Yes

Species: Nocardia wallacei

Strain Designation: N 44, TDHR 168830

Strain history: DSM 45136 <-- P. S. Conville <-- R. J. Wallace N44 <-- Texas Dept. Health, USA, TDHR 168330.

NCBI tax ID(s): 480035 (species)

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General

@ref: 12381

BacDive-ID: 10821

DSM-Number: 45136

keywords: 16S sequence, Bacteria, mesophilic

description: Nocardia wallacei N 44 is a mesophilic bacterium that builds an aerial mycelium and was isolated from sputum.

NCBI tax id

  • NCBI tax id: 480035
  • Matching level: species

strain history

@refhistory
12381<- P. S. Conville; ATCC 49873 <- R. J. Wallace <- Texas Dept. of Health, TDHR 168330
67770DSM 45136 <-- P. S. Conville <-- R. J. Wallace N44 <-- Texas Dept. Health, USA, TDHR 168330.

doi: 10.13145/bacdive10821.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Nocardiaceae
  • genus: Nocardia
  • species: Nocardia wallacei
  • full scientific name: Nocardia wallacei Conville et al. 2009

@ref: 12381

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Nocardiaceae

genus: Nocardia

species: Nocardia wallacei

full scientific name: Nocardia wallacei Conville et al. 2009

strain designation: N 44, TDHR 168830

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69374Brown beige (3012)suter with tyrosine
69374Brown beige (1011)suter without tyrosine
69374Beige red (3012)ISP 7
69374Beige red (3012)ISP 5
69374Pastel yellow (1034)ISP 3
69374Pastel yellow (1034)ISP 4
69374Pastel yellow (1034)ISP 6
69374Yellow orange (2000)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
69374yesAerial myceliumCream (9001)ISP 2
69374yesAerial myceliumCream (9001)ISP 3
69374noAerial myceliumISP 4
69374yesAerial myceliumLight ivory 81015)ISP 5
69374noAerial myceliumISP 6
69374yesAerial myceliumLight ivory (1015)ISP 7
69374yesAerial myceliumLight ivory (1015)suter with tyrosine
69374yesAerial myceliumLight ivory (1015)suter without tyrosine

pigmentation

@refproductionname
69374noMelanin
69374nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
12381https://www.dsmz.de/microorganisms/photos/DSM_45136.jpgMedium 535 28°C© Leibniz-Institut DSMZ
69374DSM_45136_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69374DSM_45136_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12381TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
12381GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12381positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

halophily

  • @ref: 69374
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6937422599arabinose-growth
6937462968cellulose-growth
6937428757fructose-growth
6937417234glucose+growth
6937417268inositol-growth
6937437684mannose-growth
6937416634raffinose-growth
6937426546rhamnose-growth
6937417992sucrose-growth
6937418222xylose-growth
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69374+--+--+-++---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69374++++/-+/-+/-+/---++/--+/--++---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
12381sputumTexasUSAUSANorth America
67770SputumTXUSAUSANorth America

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids
  • Cat3: #Sputum

Safety information

risk assessment

  • @ref: 12381
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Nocardia wallacei strain W8083 16S ribosomal RNA gene, partial sequenceGQ8530741441ena480035
20218Nocardia wallacei strain DSM 45136 16S ribosomal RNA gene, partial sequenceJN041527462ena480035
20218N.asteroides ATCC 49873 DNA for 16S rRNAZ822261318ena480035
12381Nocardia wallacei strain ATCC 49873 16S ribosomal RNA gene, partial sequenceEU0993571388ena480035

GC content

@refGC-contentmethod
1238165.0
6777065thermal denaturation, midpoint method (Tm)

External links

@ref: 12381

culture collection no.: DSM 45136, ATCC 49873, JCM 16006

straininfo link

  • @ref: 80082
  • straininfo: 42728

literature

  • topic: Phylogeny
  • Pubmed-ID: 18256227
  • title: Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the "Nocardia transvalensis Complex".
  • authors: Conville PS, Brown JM, Steigerwalt AG, Brown-Elliott BA, Witebsky FG
  • journal: J Clin Microbiol
  • DOI: 10.1128/JCM.02011-07
  • year: 2008
  • mesh: Adult, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, Heat-Shock Proteins/genetics, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Sequence Data, Nocardia/*classification/drug effects/genetics/physiology, Nocardia Infections/*microbiology, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12381Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45136)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45136
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69374Wink, J.https://cdn.dsmz.de/wink/DSM%2045136.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
80082Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42728.1StrainInfo: A central database for resolving microbial strain identifiers