Nocardia testacea (DSM 44765, IFM 0937, JCM 12235)

Name: Nocardia testacea (DSM 44765, IFM 0937, JCM 12235)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10790

Type strain

Culture col. no. DSM 44765 IFM 0937 JCM 12235 NBRC 100365 CIP 108506

NCBI tax ID(s) 1206739 248551

Special Collections DSMZ JCM CIP Wink compendium

History DSMZ <- Y. Mikami, IFM JCM <-- Y. Mikami IFM 0937. CIP <- 2004, JCM <- 2003, Y. Mikami, IFM, Japan

Links

Nocardia testacea DSM 44765 is an obligate aerobe, Gram-positive, rod-shaped bacterium that builds an aerial mycelium and was isolated from human.

Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 132792

DSM 44765,JCM 12235,NBRC 100365,CIP 108506

@ref 20215

Last LPSN update 2026-05-29 08:27:24

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Nocardiaceae

Genus Nocardia

Species Nocardia testacea

Full scientific name Nocardia testacea corrig. Kageyama et al. 2004

Synonyms (1)

Nocardia testaceus

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123482	positive	rod-shaped
125438			91.5
125438	positive		93.614

Colony morphology

@ref	Colony color	Incubation period	Medium used	Hemolysis ability
20111	Pastel orange (2003)	10-14 days	ISP 2
20111	Pastel orange (2003)	10-14 days	ISP 3
20111	Pastel orange (2003)	10-14 days	ISP 4
20111	Pastel orange (2003)	10-14 days	ISP 5
20111	Pastel orange (2003)	10-14 days	ISP 6
20111	Pastel orange (2003)	10-14 days	ISP 7
123482				0

Multicellular morphology

@ref	Complex name	Complex color	Medium name
20111	Aerial Mycelium	Beige red	ISP 2
20111			ISP 3
20111			ISP 4
20111			ISP 5
20111			ISP 6
20111			ISP 7

Multimedia

@ref:	12072
multimedia content:	DSM_44765.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44765.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12072	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
12072	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
20111	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20111	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20111	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20111	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20111	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20111	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
34959	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123482	CIP Medium 116	Medium recipe at CIP
123482	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
12072	positive	growth	28
20111	positive	optimum	28
34959	positive	growth	25
67770	positive	growth	28
123482	positive	growth	25-41
123482	negative	growth	10
123482	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
123482	obligate aerobe
125439	obligate aerobe	99.005

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123482	NaCl	positive	growth	0-4 %
123482	NaCl		growth	6 %
123482	NaCl		growth	8 %
123482	NaCl		growth	10 %

Observation

67770

Observationquinones: MK-8(H₄, ω-cycl)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
20111	22599 ChEBI	arabinose	-
20111	62968 ChEBI	cellulose	-
123482	16947 ChEBI	citrate	-	carbon source
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
123482	4853 ChEBI	esculin	-	hydrolysis
20111	28757 ChEBI	fructose	-
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
20111	17234 ChEBI	glucose	+
123482	606565 ChEBI	hippurate	-	hydrolysis
20111	29864 ChEBI	mannitol	-
20111	17268 ChEBI	myo-inositol	-
123482	17632 ChEBI	nitrate	+	reduction
123482	17632 ChEBI	nitrate	-	respiration
123482	16301 ChEBI	nitrite	-	reduction
20111	16634 ChEBI	raffinose	-
20111	26546 ChEBI	rhamnose	-
20111	17992 ChEBI	sucrose	-
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
20111	18222 ChEBI	xylose	-

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123482	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123482	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123482	15688 ChEBI	acetoin	-
123482	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123482	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123482	amylase	-
68382	beta-galactosidase	-	3.2.1.23	from API zym
123482	beta-galactosidase	-	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
123482	caseinase	-	3.4.21.50
123482	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123482	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123482	gamma-glutamyltransferase	-	2.3.2.2
123482	gelatinase	-
68379	gelatinase	-		from API Coryne
123482	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123482	lipase	-
68382	lipase (C 14)	-		from API zym
123482	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123482	ornithine decarboxylase	-	4.1.1.17
123482	oxidase	-
123482	phenylalanine ammonia-lyase	-	4.3.1.24
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
123482	tryptophan deaminase	-
123482	tween esterase	-
123482	urease	-	3.5.1.5
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
20111	not determinedn.d.	-	+	+	-	-	-	-	+	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	+	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20111	not determinedn.d.	+	+	+	-	+	+	-	+	-	+	+	-	-	-	-	+	-	-	-
123482	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host	#Human

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent
12072	human	Homo sapiens	Japan	JPN	Asia
67770	Sputum of a patient with nontuberculous mycobacterial infection	Homo sapiens	Japan	JPN	Asia
123482	Human, Sputum	Homo sapiens	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence JF797319 (>99% sequence identity) for Nocardia from Microbeatlas

Aquatic Samples	469
Soil Samples	8278
Animal Samples	943
Plant Samples	1687
Total Samples	11377

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
12072	yes, in single cases	1	Risk group (German classification) According to TRBA 466
20111		2	German classification According to TRBA 466
123482		2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM30877v1 assembly for Nocardia testacea NBRC 100365	contig	GCA_000308775	1206739.4	2551306161	1206739	23.05

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Nocardia testacea strain DSM 44765 isolate SVN09 16S ribosomal RNA gene, partial sequence	AY903612	606	248551
20218	Nocardia testacea strain DSM 44765 16S ribosomal RNA gene, partial sequence	JF797319	1412	248551
20218	Nocardia testacea gene for 16S rRNA, partial sequence	AB121769	1333	248551
20218	Nocardia testacea gene for 16S rRNA, partial sequence	AB192415	1487	248551

GC content

@ref	GC-content (mol%)	Method
67770	68.5	genome sequence analysis
67770	68.6	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM30877v1 assembly for Nocardia testacea NBRC 100365
BV-BRC: 1206739.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.01	no
125439	motility	BacteriaNetⓘ	no	89.12	no
125439	gram_stain	BacteriaNetⓘ	positive	85.06	no
125439	spore_formation	BacteriaNetⓘ	yes	70.37	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Nocardia testacea NBRC 100365
PATRIC: 1206739.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.61	no
125438	anaerobic	anaerobicⓘ	no	97.51	yes
125438	spore-forming	spore-formingⓘ	yes	59.87	no
125438	aerobic	aerobicⓘ	yes	89.51	no
125438	thermophilic	thermophileⓘ	no	97.00	yes
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Use of qPCR to monitor 2,4-dinitroanisole degrading bacteria in water and soil slurry cultures.	Waidner LA, Daniel CE, Kovar SE, Spain JC.	J Ind Microbiol Biotechnol	10.1093/jimb/kuae047	2024
Genetics	Actinomycetes as Producers of Biologically Active Terpenoids: Current Trends and Patents.	Tarasova EV, Luchnikova NA, Grishko VV, Ivshina IB.	Pharmaceuticals (Basel)	10.3390/ph16060872	2023
Phylogeny	Nocardia rhamnosiphila sp. nov., isolated from soil.	Everest GJ, Cook AE, le Roes-Hill M, Meyers PR	Syst Appl Microbiol	10.1016/j.syapm.2011.03.006	2011
Phylogeny	Nocardia testaceus sp. nov. and Nocardia senatus sp. nov., isolated from patients in Japan.	Kageyama A, Yazawa K, Nishimura K, Mikami Y	Microbiol Immunol	10.1111/j.1348-0421.2004.tb03523.x	2004

References

#12072	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44765
#20111	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34959	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123482	Collection of Institut Pasteur ; Curators of the CIP; CIP 108506
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Nocardia testacea (DSM 44765, IFM 0937, JCM 12235)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000308775

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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