Nocardia sienata DSM 44766 is an obligate aerobe, Gram-positive, rod-shaped bacterium that builds an aerial mycelium and was isolated from human.
Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Nocardia |
| Species Nocardia sienata |
| Full scientific name Nocardia sienata corrig. Kageyama et al. 2004 |
| Synonyms (1) |
| @ref: | 12073 |
| multimedia content: | DSM_44766.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_44766.jpg |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 12073 | GPHF-MEDIUM (DSMZ Medium 553) | Medium recipe at MediaDive | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water | ||
| 12073 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | ||
| 20112 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 20112 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 20112 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 20112 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 20112 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 20112 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 37436 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 122602 | CIP Medium 72 | Medium recipe at CIP | |||
| 122602 | CIP Medium 116 | Medium recipe at CIP |
| @ref | Salt | Growth | Tested relation | Concentration | |
|---|---|---|---|---|---|
| 122602 | NaCl | positive | growth | 0-10 % |
| 67770 | Observationquinones: MK-8(H4, ω-cycl) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 20112 | 22599 ChEBI | arabinose | - | ||
| 20112 | 62968 ChEBI | cellulose | - | ||
| 122602 | 16947 ChEBI | citrate | - | carbon source | |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 122602 | 4853 ChEBI | esculin | + | hydrolysis | |
| 20112 | 28757 ChEBI | fructose | + | ||
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 20112 | 17234 ChEBI | glucose | + | ||
| 122602 | 606565 ChEBI | hippurate | + | hydrolysis | |
| 20112 | 29864 ChEBI | mannitol | + | ||
| 20112 | 17268 ChEBI | myo-inositol | + | ||
| 122602 | 17632 ChEBI | nitrate | + | reduction | |
| 122602 | 17632 ChEBI | nitrate | - | respiration | |
| 122602 | 16301 ChEBI | nitrite | - | reduction | |
| 20112 | 16634 ChEBI | raffinose | - | ||
| 20112 | 26546 ChEBI | rhamnose | - | ||
| 20112 | 17992 ChEBI | sucrose | + | ||
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| 20112 | 18222 ChEBI | xylose | - |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 122602 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 122602 | alcohol dehydrogenase | + | 1.1.1.1 | |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | - | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | - | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 122602 | amylase | - | ||
| 122602 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 122602 | caseinase | - | 3.4.21.50 | |
| 122602 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 122602 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 122602 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 122602 | gelatinase | +/- | ||
| 68379 | gelatinase | - | from API Coryne | |
| 122602 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 122602 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 122602 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 122602 | ornithine decarboxylase | - | 4.1.1.17 | |
| 122602 | oxidase | - | ||
| 122602 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 122602 | protease | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 122602 | tryptophan deaminase | - | ||
| 122602 | tween esterase | - | ||
| 122602 | urease | - | 3.5.1.5 | |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| 68382 | valine arylamidase | - | from API zym |
Global distribution of 16S sequence JF797316 (>99% sequence identity) for Nocardia from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM161320v1 assembly for Nocardia sienata NBRC 100364 | contig | 1210092 | 51.77 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Nocardia sienata strain DSM 44766 16S ribosomal RNA gene, partial sequence | JF797316 | 1412 | 248552 | ||
| 20218 | Nocardia sienata strain DSM 44766 16S ribosomal RNA gene, partial sequence | JN041594 | 462 | 248552 | ||
| 20218 | Nocardia sienata gene for 16S rRNA, partial sequence | AB121770 | 1415 | 248552 | ||
| 20218 | Nocardia sienata strain X0036 16S ribosomal RNA gene, partial sequence | GQ853066 | 1439 | 248552 | ||
| 20218 | Nocardia sienata gene for 16S rRNA, partial sequence | AB516654 | 1472 | 248552 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 67770 | 68.3 | high performance liquid chromatography (HPLC) |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | yes | 50.50 | no |
| 125439 | motility | BacteriaNetⓘ | no | 76.30 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 92.40 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 98.10 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 91.98 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 96.99 | yes |
| 125438 | spore-forming | spore-formingⓘ | yes | 57.04 | no |
| 125438 | aerobic | aerobicⓘ | yes | 90.28 | no |
| 125438 | thermophilic | thermophileⓘ | no | 97.40 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 91.00 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Nocardia sienata: a new causative species of infectious keratitis. | Mosenia A, Nguyen AH, Mandel MR, Seitzman GD. | BMJ Case Rep | 10.1136/bcr-2021-247850 | 2022 | ||
| Metabolism | Production of Biosurfactant Produced from Used Cooking Oil by Bacillus sp. HIP3 for Heavy Metals Removal. | Md Badrul Hisham NH, Ibrahim MF, Ramli N, Abd-Aziz S. | Molecules | 10.3390/molecules24142617 | 2019 | |
| Phylogeny | Nocardia speluncae sp. nov., isolated from a cave. | Seo JP, Yun YW, Lee SD. | Int J Syst Evol Microbiol | 10.1099/ijs.0.65085-0 | 2007 | |
| Phylogeny | Nocardia rhamnosiphila sp. nov., isolated from soil. | Everest GJ, Cook AE, le Roes-Hill M, Meyers PR | Syst Appl Microbiol | 10.1016/j.syapm.2011.03.006 | 2011 | |
| Phylogeny | Nocardia testaceus sp. nov. and Nocardia senatus sp. nov., isolated from patients in Japan. | Kageyama A, Yazawa K, Nishimura K, Mikami Y | Microbiol Immunol | 10.1111/j.1348-0421.2004.tb03523.x | 2004 |
| #12073 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44766 |
| #20112 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #37436 | ; Curators of the CIP; |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #122602 | Collection of Institut Pasteur ; Curators of the CIP; CIP 108510 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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