Name: Neisseria meningitidis 21
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 10473

Type strain:

Species: Neisseria meningitidis

Strain Designation: 21, M 1027

Culture col. no.: DSM 10036, ATCC 13077, NCTC 10025, CCUG 3269, CIP 73.10, JCM 33544

Strain history: CIP <- 1973, ATCC <- S.E. Branham, Maryland, USA: strain M 1027 <- C. Miller, Chicago, USA: strain 21

NCBI tax ID(s): 487 (species)

SI-ID 35730

ATCC 13077, NCTC 10025, ATCC 53417, CCUG 3269, DSM 10036, CCM 4634, CNCTC 6942, CIP 73.10

Special Collections

Other strains from Neisseria meningitidis

Section

Neisseria meningitidis 21 is a microaerophile, Gram-negative, coccus-shaped bacterium that was isolated from spinal fluid from fatal case of meningitis.

coccus-shaped
Gram-negative
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Neisseriales*
	Family	*Neisseriaceae*
	Genus	*Neisseria*
	Species	**Neisseria meningitidis**
	Full Scientific Name (LPSN)	Neisseria meningitidis (Albrecht and Ghon 1901) Murray 1929 (Approved Lists 1980)

Synonym

"Micrococcus meningitidis"

Information on morphological and physiological properties Morphology

[Ref.: #120893]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.5

[Ref.: #120893]	Cell shape	coccus-shaped

[Ref.: #120893]	Motility	no

[Ref.: #3758]	Type of hemolysis	gamma
[Ref.: #3758]	Incubation period	2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3758]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3758]

Culture medium growth

[Ref.: #3758]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3758]

Culture medium composition

expand / minimize

[Ref.: #37616]

Culture medium

MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria

[Ref.: #37616]

Culture medium growth

[Ref.: #37616]

Culture medium composition

expand / minimize

[Ref.: #120893]

Culture medium

CIP Medium 10

[Ref.: #120893]

Culture medium growth

[Ref.: #120893]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3758]	growth	37 °C
[Ref.: #37616]	growth	37 °C
[Ref.: #44624]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44624]	Oxygen tolerance	microaerophile
[Ref.: #120893]	Oxygen tolerance	obligate aerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %94.411

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #120893]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #120893]	nitrite ChEBI	+	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE, API NH
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE, API NH
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120893]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH, API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH, API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68377]	alkaline phosphatase	-	3.1.3.1	* from API NH, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68377]	beta-galactosidase	-	3.2.1.23	* from API NH, API zym
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #3758]	catalase	+	1.11.1.6
[Ref.: #120893]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #3758]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #120893]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #120893]	urease	-	3.5.1.5
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH, API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #3758]	23666	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #3758]	6254	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	5829	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	5830	-	+	-	-	-	-	-	-	-	-	-	+/-	-
[Ref.: #3758]	5831	-	+	-	+/-	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	5832	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	5833	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	6040	+/-	-	+/-	+/-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6041	+	-	-	-	-	-	-	-	-	-	+/-	-	-
[Ref.: #3758]	6042	+	-	+/-	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6043	+	-	+	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6044	+	-	+	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6045	+	-	+	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	5828	-	+/-	-	+/-	+/-	-	-	-	-	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120893]	13248	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3758]	Sample type/isolated from	spinal fluid from fatal case of meningitis
[Ref.: #3758]	Geographic location (country and/or sea, region)	Chicago
[Ref.: #3758]	Country	USA
[Ref.: #3758]	Country ISO 3 Code	USA
[Ref.: #3758]	Continent	North America
[Ref.: #3758]	Geographic location	41.8818°/-87.6232°

[Ref.: #44624]	Sample type/isolated from	Human cerebrospinal fluid,fatal meningitis
[Ref.: #44624]	Sampling date	1937
[Ref.: #44624]	Geographic location (country and/or sea, region)	Chicago
[Ref.: #44624]	Country	USA
[Ref.: #44624]	Country ISO 3 Code	USA
[Ref.: #44624]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Spinal fluid from fatal case of meningitis
[Ref.: #67770]	Geographic location (country and/or sea, region)	Chicago, IL
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #120893]	Sample type/isolated from	Cerebrospinal fluid
[Ref.: #120893]	Geographic location (country and/or sea, region)	Chicago, Illinois
[Ref.: #120893]	Country	United States of America
[Ref.: #120893]	Country ISO 3 Code	USA
[Ref.: #120893]	Continent	North America
[Ref.: #120893]	Isolation date	1937
* marker position based on {}

Isolation sources categories

#Infection	#Disease	#Meningitis
#Host Body Product	#Fluids	#Cerebrospinal fluid

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3758]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120893]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3758]	N.meningitideis gene for 16S rRNA	X74900	716	GenBank ENA	487 tax ID
[Ref.: #20218]	Neisseria meningitidis 16S rRNA gene, partial	AJ247244	457	GenBank ENA	487 tax ID	*
[Ref.: #124043]	Neisseria meningitidis strain CIP 73.10 16S ribosomal RNA gene, complete sequence.	JN175351	1415	GenBank ENA	487 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Neisseria meningitidis NCTC10025	GCA_900638555	complete	GenBank ENA	487 tax ID	*
[Ref.: #66792]	Neisseria meningitidis strain NCTC10025	487.2792	complete	PATRIC	487 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Neisseria meningitidis NCTC10025 NCBI: GCA_900638555.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99.5	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	95.064	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	61.374	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	94.411	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.5	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	88.441	no

Availability in culture collections External links

[Ref.: #3758]

Culture collection no.

DSM 10036, ATCC 13077, NCTC 10025, CCUG 3269, CIP 73.10, JCM 33544

[Ref.: #79750]

SI-ID 35730

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Standardization and a multilaboratory comparison of Neisseria meningitidis serogroup A and C serum bactericidal assays. The Multilaboratory Study Group.	Maslanka SE, Gheesling LL, Libutti DE, Donaldson KB, Harakeh HS, Dykes JK, Arhin FF, Devi SJ, Frasch CE, Huang JC, Kriz-Kuzemenska P, Lemmon RD, Lorange M, Peeters CC, Quataert S, Tai JY, Carlone GM	Clin Diagn Lab Immunol	10.1128/cdli.4.2.156-167.1997	1997	*
Pathogenicity	[Value of the E-test for the determination of the susceptibility to antibiotics of Neisseria meningitidis].	Koeck JL, Cavallo JD, Fabre R, Crenn Y, Chapalain JC, Meyran M	Pathol Biol (Paris)		1994	*

References

#3758

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10036

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37616 ; Curators of the CIP;
#44624 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 3269

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#79750

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35730.1 )

#120893 Collection of Institut Pasteur ; Curators of the CIP; CIP 73.10

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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Neisseria meningitidis NCTC10025

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