Name: Neisseria meningitidis 21
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 10473

Type strain:

Species: Neisseria meningitidis

Strain Designation: 21, M 1027

Culture col. no.: DSM 10036, ATCC 13077, NCTC 10025, CCUG 3269, CIP 73.10, JCM 33544

Strain history: CIP <- 1973, ATCC <- S.E. Branham, Maryland, USA: strain M 1027 <- C. Miller, Chicago, USA: strain 21

NCBI tax ID(s): 487 (species)

SI-ID 35730

ATCC 13077, NCTC 10025, ATCC 53417, CCUG 3269, DSM 10036, CCM 4634, CNCTC 6942, CIP 73.10

Special Collections

Other strains from Neisseria meningitidis

Section

When using BacDive for research please cite our paper

Neisseria meningitidis 21 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from spinal fluid from fatal case of meningitis.

coccus-shaped
Gram-negative
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Neisseriales*
	Family	*Neisseriaceae*
	Genus	*Neisseria*
	Species	**Neisseria meningitidis**
	Full Scientific Name (LPSN)	Neisseria meningitidis (Albrecht and Ghon 1901) Murray 1929 (Approved Lists 1980)

Synonym

"Micrococcus meningitidis"

Information on morphological and physiological properties Morphology

[Ref.: #120893]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %99.5

[Ref.: #120893]	Cell shape	coccus-shaped

[Ref.: #120893]	Motility	no

[Ref.: #3758]	Type of hemolysis	gamma
[Ref.: #3758]	Incubation period	2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3758]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3758]

Culture medium growth

[Ref.: #3758]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3758]

Culture medium composition

expand / minimize

[Ref.: #37616]

Culture medium

MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria

[Ref.: #37616]

Culture medium growth

[Ref.: #37616]

Culture medium composition

expand / minimize

[Ref.: #120893]

Culture medium

CIP Medium 10

[Ref.: #120893]

Culture medium growth

[Ref.: #120893]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3758]	growth	37 °C
[Ref.: #37616]	growth	37 °C
[Ref.: #44624]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44624]	Oxygen tolerance	microaerophile
[Ref.: #120893]	Oxygen tolerance	obligate aerobe
[Ref.: #125439]	Oxygen tolerance	microaerophile Confidence in %96

[Ref.: #125439]	Ability of spore formation	no Confidence in %98.5
[Ref.: #125438]	Ability of spore formation	no Confidence in %94.411

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #120893]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #120893]	nitrite ChEBI	+	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH, API 20NE
[Ref.: #68377]	urea ChEBI	-	hydrolysis	* from API NH, API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120893]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH, API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH, API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API NH
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API NH
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #3758]	catalase	+	1.11.1.6
[Ref.: #120893]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #3758]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #120893]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #120893]	urease	-	3.5.1.5
[Ref.: #68377]	urease	-	3.5.1.5	* from API NH, API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #3758]	26346	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #3758]	6911	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	6486	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	6487	-	+	-	-	-	-	-	-	-	-	-	+/-	-
[Ref.: #3758]	6488	-	+	-	+/-	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	6489	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	6490	-	+	-	+	-	-	-	-	-	-	-	+	-
[Ref.: #3758]	6697	+/-	-	+/-	+/-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6698	+	-	-	-	-	-	-	-	-	-	+/-	-	-
[Ref.: #3758]	6699	+	-	+/-	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6700	+	-	+	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6701	+	-	+	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6702	+	-	+	-	-	-	-	-	-	-	+	-	-
[Ref.: #3758]	6485	-	+/-	-	+/-	+/-	-	-	-	-	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120893]	13248	-	-	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3758]	Sample type/isolated from	spinal fluid from fatal case of meningitis
[Ref.: #3758]	Geographic location (country and/or sea, region)	Chicago
[Ref.: #3758]	Country	USA
[Ref.: #3758]	Country ISO 3 Code	USA
[Ref.: #3758]	Continent	North America
[Ref.: #3758]	Geographic location	41.8818°/-87.6232°

[Ref.: #44624]	Sample type/isolated from	Human cerebrospinal fluid,fatal meningitis
[Ref.: #44624]	Sampling date	1937
[Ref.: #44624]	Geographic location (country and/or sea, region)	Chicago
[Ref.: #44624]	Country	USA
[Ref.: #44624]	Country ISO 3 Code	USA
[Ref.: #44624]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Spinal fluid from fatal case of meningitis
[Ref.: #67770]	Geographic location (country and/or sea, region)	Chicago, IL
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #120893]	Sample type/isolated from	Cerebrospinal fluid
[Ref.: #120893]	Geographic location (country and/or sea, region)	Chicago, Illinois
[Ref.: #120893]	Country	United States of America
[Ref.: #120893]	Country ISO 3 Code	USA
[Ref.: #120893]	Continent	North America
[Ref.: #120893]	Isolation date	1937
* marker position based on {}

Isolation sources categories

#Infection	#Disease	#Meningitis
#Host Body Product	#Fluids	#Cerebrospinal fluid

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3758]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120893]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3758]	N.meningitideis gene for 16S rRNA	X74900	716	GenBank ENA	487 tax ID
[Ref.: #20218]	Neisseria meningitidis 16S rRNA gene, partial	AJ247244	457	GenBank ENA	487 tax ID	*
[Ref.: #124043]	Neisseria meningitidis strain CIP 73.10 16S ribosomal RNA gene, complete sequence.	JN175351	1415	GenBank ENA	487 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Neisseria meningitidis NCTC10025	GCA_900638555	complete	GenBank ENA	487 tax ID	*
[Ref.: #66792]	Neisseria meningitidis strain NCTC10025	487.2792	complete	PATRIC	487 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Neisseria meningitidis NCTC10025 NCBI: GCA_900638555.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	99.5	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	95.064	yes
[Ref.: #125438]	aerobic	aerobicⓘ	no	61.374	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	94.411	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	97.5	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	88.441	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Neisseria meningitidis strain NCTC10025 PATRIC: 487.2792
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	98.5
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	78.6
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	83.4
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	microaerophile	96

Availability in culture collections External links

[Ref.: #3758]

Culture collection no.

DSM 10036, ATCC 13077, NCTC 10025, CCUG 3269, CIP 73.10, JCM 33544

[Ref.: #79750]

SI-ID 35730

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Standardization and a multilaboratory comparison of Neisseria meningitidis serogroup A and C serum bactericidal assays. The Multilaboratory Study Group.	Maslanka SE, Gheesling LL, Libutti DE, Donaldson KB, Harakeh HS, Dykes JK, Arhin FF, Devi SJ, Frasch CE, Huang JC, Kriz-Kuzemenska P, Lemmon RD, Lorange M, Peeters CC, Quataert S, Tai JY, Carlone GM	Clin Diagn Lab Immunol	10.1128/cdli.4.2.156-167.1997	1997	*
Pathogenicity	[Value of the E-test for the determination of the susceptibility to antibiotics of Neisseria meningitidis].	Koeck JL, Cavallo JD, Fabre R, Crenn Y, Chapalain JC, Meyran M	Pathol Biol (Paris)		1994	*

References

#3758

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10036

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37616 ; Curators of the CIP;
#44624 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 3269

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#79750

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35730.1 )

#120893 Collection of Institut Pasteur ; Curators of the CIP; CIP 73.10

#124043

Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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Neisseria meningitidis NCTC10025

Neisseria meningitidis strain NCTC10025

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