Strain identifier

BacDive ID: 10473

Type strain: Yes

Species: Neisseria meningitidis

Strain Designation: 21, M 1027

Strain history: CIP <- 1973, ATCC <- S.E. Branham, Maryland, USA: strain M 1027 <- C. Miller, Chicago, USA: strain 21

NCBI tax ID(s): 487 (species)

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General

@ref: 3758

BacDive-ID: 10473

DSM-Number: 10036

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, coccus-shaped

description: Neisseria meningitidis 21 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from spinal fluid from fatal case of meningitis.

NCBI tax id

  • NCBI tax id: 487
  • Matching level: species

strain history

@refhistory
3758<- ATCC <- S. E. Branham, M 1027 <- C. Miller, 21
67770CIP 73.10 <-- ATCC 13077 <-- S. E. Branham; USA; M 1027 <-- C. Miller; USA; No. 21.
120893CIP <- 1973, ATCC <- S.E. Branham, Maryland, USA: strain M 1027 <- C. Miller, Chicago, USA: strain 21

doi: 10.13145/bacdive10473.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Neisseriales
  • family: Neisseriaceae
  • genus: Neisseria
  • species: Neisseria meningitidis
  • full scientific name: Neisseria meningitidis (Albrecht and Ghon 1901) Murray 1929 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Micrococcus meningitidis

@ref: 3758

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Neisseriales

family: Neisseriaceae

genus: Neisseria

species: Neisseria meningitidis

full scientific name: Neisseria meningitidis (Albrecht and Ghon 1901) Murray 1929

strain designation: 21, M 1027

type strain: yes

Morphology

cell morphology

  • @ref: 120893
  • gram stain: negative
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 3758
  • type of hemolysis: gamma
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3758COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
37616MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
120893CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
3758positivegrowth37mesophilic
37616positivegrowth37mesophilic
44624positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44624microaerophile
120893obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12089317632nitrate-reduction
12089316301nitrite+reduction
6837718257ornithine-degradation
6837716199urea-hydrolysis
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
6836935581indoleno
12089335581indoleno

metabolite tests

@refChebi-IDmetaboliteindole test
6837735581indole-
6836935581indole-

enzymes

@refvalueactivityec
3758catalase+1.11.1.6
3758cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377lipase-
68377urease-3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
120893oxidase+
120893catalase+1.11.1.6
120893urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120893--+--+----+---------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
3758------+/--------------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3758-+-+-------+-
3758+-+-------+--
3758+-+-------+--
3758+-+-------+--
3758+-+/--------+--
3758+---------+/---
3758+/--+/-+/-------+--
3758-+-+-------+-
3758-+-+-------+-
3758-+-+/--------+-
3758-+---------+/--
3758-+-+-------+-
3758-+/--+/-+/-------+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateisolation date
3758spinal fluid from fatal case of meningitisChicagoUSAUSANorth America41.8818-87.6232
44624Human cerebrospinal fluid,fatal meningitisChicagoUSAUSANorth America1937
67770Spinal fluid from fatal case of meningitisChicago, ILUSAUSANorth America
120893Cerebrospinal fluidChicago, IllinoisUnited States of AmericaUSANorth America1937

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Meningitis
#Host Body Product#Fluids#Cerebrospinal fluid

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
37582Risk group (German classification)
1208932Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Neisseria meningitidis 16S rRNA gene, partialAJ247244457ena487
3758N.meningitideis gene for 16S rRNAX74900716ena487

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Neisseria meningitidis NCTC10025GCA_900638555completencbi487
66792Neisseria meningitidis strain NCTC10025487.2792completepatric487

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno91.421no
gram-positiveno98.429no
anaerobicno97.716no
aerobicno93.843yes
halophileno60.356no
spore-formingno97.378no
thermophileno98.838no
glucose-utilyes64.703no
motileno84.437no
glucose-fermentno89.273yes

External links

@ref: 3758

culture collection no.: DSM 10036, ATCC 13077, NCTC 10025, CCUG 3269, CIP 73.10, JCM 33544

straininfo link

  • @ref: 79750
  • straininfo: 35730

literature

topicPubmed-IDtitleauthorsjournalyearmeshDOItopic2
Pathogenicity7824314[Value of the E-test for the determination of the susceptibility to antibiotics of Neisseria meningitidis].Koeck JL, Cavallo JD, Fabre R, Crenn Y, Chapalain JC, Meyran MPathol Biol (Paris)1994Anti-Bacterial Agents/*pharmacology, Bacteriological Techniques, In Vitro Techniques, Neisseria meningitidis/*drug effects
Phylogeny9067649Standardization and a multilaboratory comparison of Neisseria meningitidis serogroup A and C serum bactericidal assays. The Multilaboratory Study Group.Maslanka SE, Gheesling LL, Libutti DE, Donaldson KB, Harakeh HS, Dykes JK, Arhin FF, Devi SJ, Frasch CE, Huang JC, Kriz-Kuzemenska P, Lemmon RD, Lorange M, Peeters CC, Quataert S, Tai JY, Carlone GMClin Diagn Lab Immunol1997Adult, Antibodies, Bacterial/blood, Bacterial Vaccines/immunology, Blood Bactericidal Activity/*immunology, Child, Preschool, Complement System Proteins/immunology, Humans, Immunosorbent Techniques, Infant, Laboratories, Meningococcal Infections/immunology/prevention & control, Middle Aged, Neisseria meningitidis/classification/*immunology, Reference Standards, Reproducibility of Results, Serotyping, Species Specificity10.1128/cdli.4.2.156-167.1997Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3758Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10036)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10036
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37616Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10662
44624Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 3269)https://www.ccug.se/strain?id=3269
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68377Automatically annotated from API NH
68382Automatically annotated from API zym
79750Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35730.1StrainInfo: A central database for resolving microbial strain identifiers
120893Curators of the CIPCollection of Institut Pasteur (CIP 73.10)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2073.10