Aquaspirillum serpens (DSM 9156, ATCC 27641, IFO 15465)

Name: Aquaspirillum serpens (DSM 9156, ATCC 27641, IFO 15465)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10436

Type strain

Strain Designation Kh-4

Culture col. no. DSM 9156 ATCC 27641 IFO 15465 NBRC 15465 LMG 6234 4 more

NCBI tax ID(s) 190

Special Collections DSMZ JCM CIP

History DSMZ <- ATCC <- A. Banerjee, Kh-4 JCM <-- IAM 14967 <-- IFO 15465 <-- ATCC 27641 <-- A. Banerjee Kh-4. CIP <- 1998, ATCC <- A. Banerjee: strain Kh-4

Links

Aquaspirillum serpens Kh-4 is a Gram-negative, motile, rod-shaped bacterium that was isolated from freshwater pond.

Gram-negative motile rod-shaped 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13576

LMG 6234,ATCC 27641,CIP 105799,DSM 9156,IFO 15465,IAM 14967,NBRC 15465,DSM 91565,JCM 21439

@ref 20215

Last LPSN update 2026-05-29 08:27:20

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Neisseriales

Family Aquaspirillaceae

Genus Aquaspirillum

Species Aquaspirillum serpens

Full scientific name Aquaspirillum serpens (Müller 1786) Hylemon et al. 1973 (Approved Lists 1980)

Synonyms (1)

"Vibrio serpens"

BacDive ID	Other strains from **Aquaspirillum serpens** (1)	Type strain
10435	A. serpens DSM 68, ATCC 12638, CCUG 2092, NCTC 10593, ... (type strain)

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
122573	negative	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
39360	MEDIUM 14 - Williams and Rittenberg medium for Aquaspirillum genus		Distilled water make up to (1000.000 ml);Agar (13.000 g);Yeast extract (3.000 g);Peptone (5.000 g);Beef extract (3.000 g)
3492	PEPTONE-SUCCINATE AGAR (DSMZ Medium 653)	Medium recipe at MediaDive	Name: PEPTONE-SUCCINATE AGAR (DSMZ Medium 653) Composition: Peptone 5.0 g/l Succinic acid 1.68 g/l Agar 1.5 g/l MgSO4 x 7 H2O 1.0 g/l (NH4)2SO4 1.0 g/l FeCl3 x 6 H2O 0.002 g/l MnSO4 x H2O 0.002 g/l Distilled water
122573	CIP Medium 14	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3492	positive	growth	30
39360	positive	growth	25
67770	positive	growth	30
122573	positive	growth	15-41
122573	negative	growth	5

Physiology and metabolism

Halophily

@ref	Salt	Growth	Tested relation	Concentration
122573	NaCl	positive	growth	0 %
122573	NaCl		growth	2 %
122573	NaCl		growth	4 %
122573	NaCl		growth	6 %
122573	NaCl		growth	8 %
122573	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
122573	16947 ChEBI	citrate	-	carbon source
122573	4853 ChEBI	esculin	-	hydrolysis
122573	17234 ChEBI	glucose	-	fermentation
122573	17234 ChEBI	glucose	-	degradation
122573	17716 ChEBI	lactose	-	fermentation
122573	17632 ChEBI	nitrate	-	reduction
122573	17632 ChEBI	nitrate	-	respiration
122573	16301 ChEBI	nitrite	-	reduction
122573	132112 ChEBI	sodium thiosulfate	-	builds gas from

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
122573	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122573	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
122573	15688 ChEBI	acetoin	-
122573	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122573	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122573	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
122573	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122573	gamma-glutamyltransferase	+	2.3.2.2
122573	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
122573	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122573	ornithine decarboxylase	-	4.1.1.17
122573	oxidase	+
122573	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
122573	tryptophan deaminase	-
122573	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122573	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122573	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Environmental	#Aquatic	#Pond (small)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3492	freshwater pond
67770	Freshwater pond	West Bengal	India	IND	Asia
122573	Environment, Freshwater pond	West Bengal	India	IND	Asia

Taxonmaps

69479

Global distribution of 16S sequence AB680863 (>99% sequence identity) for Aquaspirillum serpens subclade from Microbeatlas

Aquatic Samples	9687
Soil Samples	408
Animal Samples	1624
Plant Samples	108
Total Samples	11827

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3492	1	Risk group (German classification) According to TRBA 466
122573	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Aquaspirillum serpens gene for 16S rRNA, partial sequence, strain: NBRC 15465	AB680863	1461		190

GC content

@ref	GC-content (mol%)	Method
67770	51	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Title	Authors	Journal	DOI	Year
Biology of the chemoheterotrophic spirilla.	Krieg NR.	Bacteriol Rev	10.1128/br.40.1.55-115.1976	1976

References

#3492	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 9156
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39360	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122573	Collection of Institut Pasteur ; Curators of the CIP; CIP 105799
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Aquaspirillum serpens (DSM 9156, ATCC 27641, IFO 15465)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media